Restriction map for
pFN21AE5841
(Circular) (Six-base) MAPSORT of: pFN21AE5841.seq Check: 392 from: 1 to: 10099
pFN21AE5841 10099 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2256 3655 3724 5330 6935 6962 7363 7400 2256
Size: 1399 69 1606 1605 27 401 37 4955
Fragments arranged by size:
4955 1606 1605 1399 401 69 37 27
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7909 278
Size: 53 83 186 936 6373 2468
Fragments arranged by size:
6373 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2758 3090 7442 1937
Size: 821 332 4352 4594
Fragments arranged by size:
4594 4352 821 332
Acc65I G'GTAC_C
Cuts at: 7420 7420
Size: 10099
AclI AA'CG_TT
Cuts at: 1563 8226 8599 1563
Size: 6663 373 3063
Fragments arranged by size:
6663 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2664 4103 4815 6630 8162 9546 9814 1632
Size: 1032 1439 712 1815 1532 1384 268 1917
Fragments arranged by size:
1917 1815 1532 1439 1384 1032 712 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9902
AflIII A'CryG_T
Cuts at: 1203 2763 2951 3331 8998 9975 1203
Size: 1560 188 380 5667 977 1327
Fragments arranged by size:
5667 1560 1327 977 380 188
AgeI A'CCGG_T
Cuts at: 8930 8930
Size: 10099
AhdI GACnn_n'nnGTC
Cuts at: 8828 8828
Size: 10099
AleI CACnn'nnGTG
Cuts at: 1166 5352 5464 6620 1166
Size: 4186 112 1156 4645
Fragments arranged by size:
4645 4186 1156 112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4032 4675 5442 7011 1184
Size: 2848 643 767 1569 4272
Fragments arranged by size:
4272 2848 1569 767 643
AlwNI CAG_nnn'CTG
Cuts at: 2044 2719 3440 3826 3944 4411 4530 5697 6667
Size: 675 721 386 118 467 119 1167 970
Cuts at: 6667 6763 6893 6919 7842 9414 2044
Size: 96 130 26 923 1572 2729
Fragments arranged by size:
2729 1572 1167 970 923 721 675 467
386 130 119 118 96 26
ApaI G_GGCC'C
Cuts at: 5225 6373 6478 5225
Size: 1148 105 8846
Fragments arranged by size:
8846 1148 105
ApaLI G'TGCA_C
Cuts at: 2880 2921 8156 9312 2880
Size: 41 5235 1156 3667
Fragments arranged by size:
5235 3667 1156 41
ApoI r'AATT_y
Cuts at: 1493 7408 7634 7786 9895 1493
Size: 5915 226 152 2109 1697
Fragments arranged by size:
5915 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8653 160
Size: 872 7621 1606
Fragments arranged by size:
7621 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10099
AvaI C'yCGr_G
Cuts at: 1870 1943 3726 3767 5840 7218 7424 1870
Size: 73 1783 41 2073 1378 206 4545
Fragments arranged by size:
4545 2073 1783 1378 206 73 41
BamHI G'GATC_C
Cuts at: 3211 3470 3595 4990 7429 7796 3211
Size: 259 125 1395 2439 367 5514
Fragments arranged by size:
5514 2439 1395 367 259 125
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2286 2896 3153 3976 4267
Size: 325 217 627 499 610 257 823 291
Cuts at: 4267 4340 4434 4568 4950 5678 5741 5987 6238
Size: 73 94 134 382 728 63 246 251
Cuts at: 6238 6278 6872 7274 7420 8875 618
Size: 40 594 402 146 1455 1842
Fragments arranged by size:
1842 1455 823 728 627 610 594 499
402 382 325 291 257 251 246 217
146 134 94 73 63 40
BanII G_rGCy'C
Cuts at: 1391 3168 3381 3891 5225 5397 6373 6478 6633
Size: 1777 213 510 1334 172 976 105 155
Cuts at: 6633 6968 6985 7418 1391
Size: 335 17 433 4072
Fragments arranged by size:
4072 1777 1334 976 510 433 335 213
172 155 105 17
BbeI G_GCGC'C
Cuts at: 2290 3157 4438 2290
Size: 867 1281 7951
Fragments arranged by size:
7951 1281 867
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 9128
BbvCI CC'TCA_GC
Cuts at: 2910 3012 3717 5755 6052 6323 6425 6795 2910
Size: 102 705 2038 297 271 102 370 6214
Fragments arranged by size:
6214 2038 705 370 297 271 102 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8290 8324 1903
Size: 34 6353 34 3678
Fragments arranged by size:
6353 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2402 7991 9201 2402
Size: 5589 1210 3300
Fragments arranged by size:
5589 3300 1210
BclI T'GATC_A
Cuts at: 1548 4696 7126 1548
Size: 3148 2430 4521
Fragments arranged by size:
4521 3148 2430
BfrBI ATG'CAT
Cuts at: 5579 5579
Size: 10099
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2096 2217 2894
Size: 107 122 71 631 605 424 121 677
Cuts at: 2894 3366 3525 3534 4482 4602 4878 5358 6851
Size: 472 159 9 948 120 276 480 1493
Cuts at: 6851 7094 8710 136
Size: 243 1616 1525
Fragments arranged by size:
1616 1525 1493 948 677 631 605 480
472 424 276 243 159 122 121 120
107 71 9
BglII A'GATC_T
Cuts at: 7380 10094 7380
Size: 2714 7385
BlpI GC'TnA_GC
Cuts at: 2075 2497 3380 6651 7098 7155 7516 2075
Size: 422 883 3271 447 57 361 4658
Fragments arranged by size:
4658 3271 883 447 422 361 57
Bme1580I G_kGCm'C
Cuts at: 1790 2884 2899 2925 3060 3344 3434 3617 3981
Size: 1094 15 26 135 284 90 183 364
Cuts at: 3981 4111 4272 4345 4456 4519 4912 4920 5065
Size: 130 161 73 111 63 393 8 145
Cuts at: 5065 5138 5156 5201 5225 5245 5536 5746 6172
Size: 73 18 45 24 20 291 210 426
Cuts at: 6172 6241 6373 6403 6478 6856 6961 7078 7090
Size: 69 132 30 75 378 105 117 12
Cuts at: 7090 7300 8160 9316 1790
Size: 210 860 1156 2573
Fragments arranged by size:
2573 1156 1094 860 426 393 378 364
291 284 210 210 183 161 145 135
132 130 117 111 105 90 75 73
73 69 63 45 30 26 24 20
18 15 12 8
BmgBI CAC'GTC
Cuts at: 1318 3503 3580 1318
Size: 2185 77 7837
Fragments arranged by size:
7837 2185 77
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2072 2602 3305
Size: 416 53 83 399 24 655 530 703
Cuts at: 3305 3541 3595 5410 6371 7312 7830 8788 442
Size: 236 54 1815 961 941 518 958 1753
Fragments arranged by size:
1815 1753 961 958 941 703 655 530
518 416 399 236 83 54 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10099
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 10099
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 3848 4238 4443 5924 6857 7025 7322
Size: 1202 1407 390 205 1481 933 168 297
Cuts at: 7322 8759 1239
Size: 1437 2579
Fragments arranged by size:
2579 1481 1437 1407 1202 933 390 297
205 168
Bpu10I CC'TnA_GC
Cuts at: 2910 3012 3539 3717 3793 3900 5755 6052 6203
Size: 102 527 178 76 107 1855 297 151
Cuts at: 6203 6323 6425 6795 7239 2910
Size: 120 102 370 444 5770
Fragments arranged by size:
5770 1855 527 444 370 297 178 151
120 107 102 102 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2002 3240 4073 4350 8224 9089 9387 2002
Size: 1238 833 277 3874 865 298 2714
Fragments arranged by size:
3874 2714 1238 865 833 298 277
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3310 5400 8762 882
Size: 617 1811 2090 3362 2219
Fragments arranged by size:
3362 2219 2090 1811 617
BsaAI yAC'GTr
Cuts at: 493 1204 2843 2954 6728 7890 493
Size: 711 1639 111 3774 1162 2702
Fragments arranged by size:
3774 2702 1639 1162 711 111
BsaBI GATnn'nnATC
Cuts at: 2985 10093 2985
Size: 7108 2991
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2287 3004 3154 4435
Size: 53 83 186 936 754 717 150 1281
Cuts at: 4435 7906 8288 8923 275
Size: 3471 382 635 1451
Fragments arranged by size:
3471 1451 1281 936 754 717 635 382
186 150 83 53
BsaWI w'CCGG_w
Cuts at: 6693 8532 8930 9204 9351 9762 6693
Size: 1839 398 274 147 411 7030
Fragments arranged by size:
7030 1839 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2444 1178
Size: 30 165 30 1011 30 8833
Fragments arranged by size:
8833 1011 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4271 4298 5807 6854 1186
Size: 3085 27 1509 1047 4431
Fragments arranged by size:
4431 3085 1509 1047 27
BseYI C'CCAG_C
Cuts at: 2223 3149 4110 4271 4851 5131 5674 5716 6640
Size: 926 961 161 580 280 543 42 924
Cuts at: 6640 6789 6846 9302 9798 2223
Size: 149 57 2456 496 2524
Fragments arranged by size:
2524 2456 961 926 924 580 543 496
280 161 149 57 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3276 3950 4522 4987 5101 5627 5747
Size: 573 1540 674 572 465 114 526 120
Cuts at: 5747 5754 6969 7167 7397 1163
Size: 7 1215 198 230 3865
Fragments arranged by size:
3865 1540 1215 674 573 572 526 465
230 198 120 114 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7549 8310 9338 1540
Size: 275 186 5548 761 1028 2301
Fragments arranged by size:
5548 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2884 2925 3168 3449 3741 4716 5397 5829 6097
Size: 41 243 281 292 975 681 432 268
Cuts at: 6097 6633 6968 8160 8245 9316 2884
Size: 536 335 1192 85 1071 3667
Fragments arranged by size:
3667 1192 1071 975 681 536 432 335
292 281 268 243 85 41
BsmI GAATG_Cn'
Cuts at: 7610 7703 7610
Size: 93 10006
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2884 2899 2925 3060 3168 3344 3381
Size: 399 1094 15 26 135 108 176 37
Cuts at: 3381 3434 3449 3617 3741 3891 3981 4111 4272
Size: 53 15 168 124 150 90 130 161
Cuts at: 4272 4345 4456 4519 4716 4912 4920 5065 5138
Size: 73 111 63 197 196 8 145 73
Cuts at: 5138 5156 5201 5225 5245 5397 5536 5746 5829
Size: 18 45 24 20 152 139 210 83
Cuts at: 5829 6097 6172 6241 6373 6403 6478 6633 6856
Size: 268 75 69 132 30 75 155 223
Cuts at: 6856 6961 6968 6985 7078 7090 7300 7418 8160
Size: 105 7 17 93 12 210 118 742
Cuts at: 8160 8245 9316 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1094 1071 742 399 268 223 210
210 197 196 176 168 161 155 152
150 145 139 135 132 130 124 118
111 108 105 93 90 85 83 75
75 73 73 69 63 53 45 37
30 26 24 20 18 17 15 15
12 8 7
BspEI T'CCGG_A
Cuts at: 6693 9762 6693
Size: 3069 7030
BspHI T'CATG_A
Cuts at: 7988 9718 7988
Size: 1730 8369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 2256 3003 3213 3558 3655 3724 3879
Size: 1328 84 747 210 345 97 69 155
Cuts at: 3879 5136 5330 6935 6962 7363 7400 7454 844
Size: 1257 194 1605 27 401 37 54 3489
Fragments arranged by size:
3489 1605 1328 1257 747 401 345 210
194 155 97 84 69 54 37 27
BsrBI CCG'CTC
Cuts at: 1394 1823 3228 6955 7238 7294 7986 1394
Size: 429 1405 3727 283 56 692 3507
Fragments arranged by size:
3727 3507 1405 692 429 283 56
BsrDI GCAATG_nn'
Cuts at: 65 1248 3112 3362 3911 4556 4648 4728 5027
Size: 1183 1864 250 549 645 92 80 299
Cuts at: 5027 6337 8594 8768 65
Size: 1310 2257 174 1396
Fragments arranged by size:
2257 1864 1396 1310 1183 645 549 299
250 174 92 80
BsrFI r'CCGG_y
Cuts at: 1700 1810 3405 4626 4776 4947 5357 6281 8743
Size: 110 1595 1221 150 171 410 924 2462
Cuts at: 8743 8930 1700
Size: 187 2869
Fragments arranged by size:
2869 2462 1595 1221 924 410 187 171
150 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10099
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10099
BssSI C'ACGA_G
Cuts at: 4508 5445 8159 9171 4508
Size: 937 2714 1012 5436
Fragments arranged by size:
5436 2714 1012 937
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3348 3976 5026 5656 6257 6763 7073 794
Size: 2554 628 1050 630 601 506 310 3820
Fragments arranged by size:
3820 2554 1050 630 628 601 506 310
BstBI TT'CG_AA
Cuts at: 8903 8903
Size: 10099
BstEII G'GTnAC_C
Cuts at: 1184 2401 3760 1184
Size: 1217 1359 7523
Fragments arranged by size:
7523 1359 1217
BstXI CCAn_nnnn'nTGG
Cuts at: 2413 2488 2592 2413
Size: 75 104 9920
Fragments arranged by size:
9920 104 75
BstYI r'GATC_y
Cuts at: 1972 2799 3211 3470 3595 4990 6657 7380 7429
Size: 827 412 259 125 1395 1667 723 49
Cuts at: 7429 7796 8181 8198 9650 9736 9748 10094 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1667 1452 1395 827 723 412 385
367 346 259 125 86 49 17 12
BstZ17I GTA'TAC
Cuts at: 3091 3091
Size: 10099
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 2585 2789 3052 3246 4741
Size: 552 939 150 431 204 263 194 1495
Cuts at: 4741 4751 5083 5272 5536 513
Size: 10 332 189 264 5076
Fragments arranged by size:
5076 1495 939 552 431 332 264 263
204 194 189 150 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2306 3424 4909 487
Size: 683 56 1080 1118 1485 5677
Fragments arranged by size:
5677 1485 1118 1080 683 56
BtsI GCAGTG_nn'
Cuts at: 2978 3628 4867 4975 5065 5156 5194 5201 5347
Size: 650 1239 108 90 91 38 7 146
Cuts at: 5347 5348 5426 5485 5766 5933 6894 7078 7615
Size: 1 78 59 281 167 961 184 537
Cuts at: 7615 8409 8429 2978
Size: 794 20 4648
Fragments arranged by size:
4648 1239 961 794 650 537 281 184
167 146 108 91 90 78 59 38
20 7 1
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10099
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2935 2970 3094 3129 3689 3724 529
Size: 35 2371 35 124 35 560 35 6904
Fragments arranged by size:
6904 2371 560 124 35 35 35 35
DraI TTT'AAA
Cuts at: 7403 7403
Size: 10099
DraIII CAC_nnn'GTG
Cuts at: 5060 5897 6257 5060
Size: 837 360 8902
Fragments arranged by size:
8902 837 360
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5106 9106 809
Size: 507 3790 4000 1802
Fragments arranged by size:
4000 3790 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2509 3019 3084
Size: 54 1425 50 275 18 679 510 65
Cuts at: 3084 3310 3526 4603 4628 4945 5269 6156 6612
Size: 226 216 1077 25 317 324 887 456
Cuts at: 6612 7546 8435 10064 8
Size: 934 889 1629 43
Fragments arranged by size:
1629 1425 1077 934 889 887 679 510
456 324 317 275 226 216 65 54
50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 7546 1537
Size: 275 5734 4090
Fragments arranged by size:
5734 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 8029 1353
Size: 6676 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5386 8686 9060 9206 387
Size: 1064 138 3797 3300 374 146 1280
Fragments arranged by size:
3797 3300 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3166 5395 6631 6966 3166
Size: 2229 1236 335 6299
Fragments arranged by size:
6299 2229 1236 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 2664 3848 4103 4238 4443 4815
Size: 393 809 223 1184 255 135 205 372
Cuts at: 4815 5924 6630 6857 7025 7322 8162 8759 9546
Size: 1109 706 227 168 297 840 597 787
Cuts at: 9546 9814 1239
Size: 268 1524
Fragments arranged by size:
1524 1184 1109 840 809 787 706 597
393 372 297 268 255 227 223 205
168 135
EcoNI CCTnn'n_nnAGG
Cuts at: 6491 6575 6923 6491
Size: 84 348 9667
Fragments arranged by size:
9667 348 84
EcoO109I rG'GnC_Cy
Cuts at: 2104 3202 3535 3895 3941 4747 4799 4927 5221
Size: 1098 333 360 46 806 52 128 294
Cuts at: 5221 5222 5315 6319 7147 7222 2104
Size: 1 93 1004 828 75 4981
Fragments arranged by size:
4981 1098 1004 828 806 360 333 294
128 93 75 52 46 1
EcoRI G'AATT_C
Cuts at: 7408 7786 7408
Size: 378 9721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 5095 6154 2284
Size: 2811 1059 6229
Fragments arranged by size:
6229 2811 1059
FspI TGC'GCA
Cuts at: 4300 7346 8605 4300
Size: 3046 1259 5794
Fragments arranged by size:
5794 3046 1259
HaeII r_GCGC'y
Cuts at: 1678 2290 3157 3757 4225 4438 4680 5635 9246
Size: 612 867 600 468 213 242 955 3611
Cuts at: 9246 1678
Size: 2531
Fragments arranged by size:
3611 2531 955 867 612 600 468 242
213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2919 2951 5426 5458 6109
Size: 32 135 32 1347 32 2475 32 651
Cuts at: 6109 6141 8740 8772 8814 8846 1373
Size: 32 2599 32 42 32 2626
Fragments arranged by size:
2626 2599 2475 1347 651 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 7443 7689 669
Size: 988 281 5505 246 3079
Fragments arranged by size:
5505 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4902 748
Size: 4154 5945
HpaI GTT'AAC
Cuts at: 7689 7689
Size: 10099
KasI G'GCGC_C
Cuts at: 2286 3153 4434 2286
Size: 867 1281 7951
Fragments arranged by size:
7951 1281 867
KpnI G_GTAC'C
Cuts at: 7424 7424
Size: 10099
MfeI C'AATT_G
Cuts at: 7698 7698
Size: 10099
MluI A'CGCG_T
Cuts at: 9975 9975
Size: 10099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3897 6972 7302 7311 9213 9397 1305
Size: 2592 3075 330 9 1902 184 2007
Fragments arranged by size:
3075 2592 2007 1902 330 184 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3528 4605 5271 6158 6614
Size: 54 1425 343 1696 1077 666 887 456
Cuts at: 6614 10
Size: 3495
Fragments arranged by size:
3495 1696 1425 1077 887 666 456 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 2595 3027 4354 4653 4839 5352
Size: 648 1320 109 432 1327 299 186 513
Cuts at: 5352 5430 5464 6605 6620 8057 8416 8575 518
Size: 78 34 1141 15 1437 359 159 2042
Fragments arranged by size:
2042 1437 1327 1320 1141 648 513 432
359 299 186 159 109 78 34 15
MspA1I CmG'CkG
Cuts at: 1606 2359 2413 3069 3149 3516 4440 4593 5115
Size: 753 54 656 80 367 924 153 522
Cuts at: 5115 5238 5493 5644 6166 6327 6341 6397 6472
Size: 123 255 151 522 161 14 56 75
Cuts at: 6472 6650 6850 7105 7255 7515 8192 9340 9585
Size: 178 200 255 150 260 677 1148 245
Cuts at: 9585 1606
Size: 2120
Fragments arranged by size:
2120 1148 924 753 677 656 522 522
367 260 255 255 245 200 178 161
153 151 150 123 80 75 56 54
14
NaeI GCC'GGC
Cuts at: 3407 4628 4778 5359 6283 3407
Size: 1221 150 581 924 7223
Fragments arranged by size:
7223 1221 924 581 150
NarI GG'CG_CC
Cuts at: 2287 3154 4435 2287
Size: 867 1281 7951
Fragments arranged by size:
7951 1281 867
NcoI C'CATG_G
Cuts at: 513 1065 2004 3052 3246 5083 5272 513
Size: 552 939 1048 194 1837 189 5340
Fragments arranged by size:
5340 1837 1048 939 552 194 189
NdeI CA'TA_TG
Cuts at: 387 4762 387
Size: 4375 5724
NgoMIV G'CCGG_C
Cuts at: 3405 4626 4776 5357 6281 3405
Size: 1221 150 581 924 7223
Fragments arranged by size:
7223 1221 924 581 150
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10099
NotI GC'GGCC_GC
Cuts at: 7546 7546
Size: 10099
NsiI A_TGCA'T
Cuts at: 5581 5581
Size: 10099
NspI r_CATG'y
Cuts at: 1132 1229 2525 3335 4019 4652 5124 5583 5707
Size: 97 1296 810 684 633 472 459 124
Cuts at: 5707 6011 7457 9002 1132
Size: 304 1446 1545 2229
Fragments arranged by size:
2229 1545 1446 1296 810 684 633 472
459 304 124 97
PasI CC'CwG_GG
Cuts at: 1348 2049 2891 4802 4913 5411 5501 6868 7078
Size: 701 842 1911 111 498 90 1367 210
Cuts at: 7078 1348
Size: 4369
Fragments arranged by size:
4369 1911 1367 842 701 498 210 111
90
PciI A'CATG_T
Cuts at: 3331 8998 3331
Size: 5667 4432
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3643 5189 5253 6667 1108
Size: 2535 1546 64 1414 4540
Fragments arranged by size:
4540 2535 1546 1414 64
PfoI T'CCnGG_A
Cuts at: 3693 3782 7002 3693
Size: 89 3220 6790
Fragments arranged by size:
6790 3220 89
PmeI GTTT'AAAC
Cuts at: 7403 7403
Size: 10099
PmlI CAC'GTG
Cuts at: 2954 2954
Size: 10099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8168 8200 9681 9713 8168
Size: 32 1481 32 8554
Fragments arranged by size:
8554 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3202 3895 4747 4927 7222 3202
Size: 693 852 180 2295 6079
Fragments arranged by size:
6079 2295 852 693 180
PshAI GACnn'nnGTC
Cuts at: 1613 3264 1613
Size: 1651 8448
PsiI TTA'TAA
Cuts at: 7669 7669
Size: 10099
PspOMI G'GGCC_C
Cuts at: 5221 6369 6474 5221
Size: 1148 105 8846
Fragments arranged by size:
8846 1148 105
PspXI vC'TCGA_Gb
Cuts at: 5840 5840
Size: 10099
PstI C_TGCA'G
Cuts at: 830 2253 5492 6028 6396 6768 6973 7254 7451
Size: 1423 3239 536 368 372 205 281 197
Cuts at: 7451 830
Size: 3478
Fragments arranged by size:
3478 3239 1423 536 372 368 281 205
197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10099
PvuII CAG'CTG
Cuts at: 2413 3069 3149 5493 6327 6650 7105 2413
Size: 656 80 2344 834 323 455 5407
Fragments arranged by size:
5407 2344 834 656 455 323 80
SacI G_AGCT'C
Cuts at: 3168 5397 6633 6968 3168
Size: 2229 1236 335 6299
Fragments arranged by size:
6299 2229 1236 335
SalI G'TCGA_C
Cuts at: 1936 7441 1936
Size: 5505 4594
SanDI GG'GwC_CC
Cuts at: 4747 4927 4747
Size: 180 9919
SbfI CC_TGCA'GG
Cuts at: 2253 7451 2253
Size: 5198 4901
ScaI AGT'ACT
Cuts at: 2813 8347 10059 2813
Size: 5534 1712 2853
Fragments arranged by size:
5534 2853 1712
SexAI A'CCwGG_T
Cuts at: 6924 6924
Size: 10099
SfcI C'TryA_G
Cuts at: 826 1045 2249 2698 3034 3809 5488 6024 6345
Size: 219 1204 449 336 775 1679 536 321
Cuts at: 6345 6392 6732 6764 6969 7050 7250 7259 7447
Size: 47 340 32 205 81 200 9 188
Cuts at: 7447 8582 9263 9454 826
Size: 1135 681 191 1471
Fragments arranged by size:
1679 1471 1204 1135 775 681 536 449
340 336 321 219 205 200 191 188
81 47 32 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2217 3534 2217
Size: 1317 8782
SfoI GGC'GCC
Cuts at: 2288 3155 4436 2288
Size: 867 1281 7951
Fragments arranged by size:
7951 1281 867
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10099
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10099
SmaI CCC'GGG
Cuts at: 1872 3769 7426 1872
Size: 1897 3657 4545
Fragments arranged by size:
4545 3657 1897
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2017 3255 4052 4365 5840 8203
Size: 197 926 74 1238 797 313 1475 2363
Cuts at: 8203 9104 9366 820
Size: 901 262 1553
Fragments arranged by size:
2363 1553 1475 1238 926 901 797 313
262 197 74
SnaBI TAC'GTA
Cuts at: 493 7890 493
Size: 7397 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10099
SphI G_CATG'C
Cuts at: 1132 4652 5583 6011 7457 1132
Size: 3520 931 428 1446 3774
Fragments arranged by size:
3774 3520 1446 931 428
SspI AAT'ATT
Cuts at: 5 52 4847 8023 5
Size: 47 4795 3176 2081
Fragments arranged by size:
4795 3176 2081 47
StuI AGG'CCT
Cuts at: 1517 4295 1517
Size: 2778 7321
StyI C'CwwG_G
Cuts at: 513 1065 2004 3052 3198 3246 3981 3997 5083
Size: 552 939 1048 146 48 735 16 1086
Cuts at: 5083 5272 7168 7538 513
Size: 189 1896 370 3074
Fragments arranged by size:
3074 1896 1086 1048 939 735 552 370
189 146 48 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3077 3572 4682 5313 5613 5666 5975
Size: 1255 1497 495 1110 631 300 53 309
Cuts at: 5975 7112 8126 8143 8296 8481 8926 325
Size: 1137 1014 17 153 185 445 1498
Fragments arranged by size:
1498 1497 1255 1137 1110 1014 631 495
445 309 300 185 153 53 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2811 6823 8345
Size: 274 80 33 51 1443 834 4012 1522
Cuts at: 8345 10057 96
Size: 1712 138
Fragments arranged by size:
4012 1712 1522 1443 834 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3587 3619 5035 5067 6931 6963 10059 10091 3587
Size: 32 1416 32 1864 32 3096 32 3595
Fragments arranged by size:
3595 3096 1864 1416 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10099
XbaI T'CTAG_A
Cuts at: 7435 7435
Size: 10099
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3644 3962 5072 5534 5894 6170 6514 3644
Size: 318 1110 462 360 276 344 7229
Fragments arranged by size:
7229 1110 462 360 344 318 276
XhoI C'TCGA_G
Cuts at: 1943 5840 1943
Size: 3897 6202
XmaI C'CCGG_G
Cuts at: 1870 3767 7424 1870
Size: 1897 3657 4545
Fragments arranged by size:
4545 3657 1897
XmnI GAAnn'nnTTC
Cuts at: 2267 6566 8228 2267
Size: 4299 1662 4138
Fragments arranged by size:
4299 4138 1662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7907 276
Size: 53 83 186 936 6373 2468
Fragments arranged by size:
6373 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BsmBI Bsu36I
EcoRV FseI FspAI NruI PacI PsrI RsrII SacII
SapI SrfI SwaI