Restriction map for
pFN21AE5834
(Circular) (Six-base) MAPSORT of: pFN21AE5834.seq Check: 2393 from: 1 to: 6082
pFN21AE5834 6082 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3115 3152 3115
Size: 37 6045
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3892 278
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2968 3425 1937
Size: 1031 457 4594
Fragments arranged by size:
4594 1031 457
Acc65I G'GTAC_C
Cuts at: 3403 3403
Size: 6082
AclI AA'CG_TT
Cuts at: 1563 4209 4582 1563
Size: 2646 373 3063
Fragments arranged by size:
3063 2646 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2304 3059 4145 5529 5797 1632
Size: 672 755 1086 1384 268 1917
Fragments arranged by size:
1917 1384 1086 755 672 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5885
AflIII A'CryG_T
Cuts at: 1203 4981 5958 1203
Size: 3778 977 1327
Fragments arranged by size:
3778 1327 977
AgeI A'CCGG_T
Cuts at: 4913 4913
Size: 6082
AhdI GACnn_n'nnGTC
Cuts at: 4811 4811
Size: 6082
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6082
AlwNI CAG_nnn'CTG
Cuts at: 3825 5397 3825
Size: 1572 4510
ApaLI G'TGCA_C
Cuts at: 3099 4139 5295 3099
Size: 1040 1156 3886
Fragments arranged by size:
3886 1156 1040
ApoI r'AATT_y
Cuts at: 1493 3391 3617 3769 5878 1493
Size: 1898 226 152 2109 1697
Fragments arranged by size:
2109 1898 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4636 160
Size: 872 3604 1606
Fragments arranged by size:
3604 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
AvaI C'yCGr_G
Cuts at: 1870 1943 2790 2901 3199 3370 3407 1870
Size: 73 847 111 298 171 37 4545
Fragments arranged by size:
4545 847 298 171 111 73 37
BamHI G'GATC_C
Cuts at: 3412 3779 3412
Size: 367 5715
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2388 3403 4858 618
Size: 325 217 627 601 1015 1455 1842
Fragments arranged by size:
1842 1455 1015 627 601 325 217
BanII G_rGCy'C
Cuts at: 1391 3401 1391
Size: 2010 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4273 4307 1903
Size: 34 2336 34 3678
Fragments arranged by size:
3678 2336 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2399 2873 2936 3974 5184 2399
Size: 474 63 1038 1210 3297
Fragments arranged by size:
3297 1210 1038 474 63
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6082
BfrBI ATG'CAT
Cuts at: 2720 2831 2720
Size: 111 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4693 136
Size: 107 122 71 631 605 3021 1525
Fragments arranged by size:
3021 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2333 6077 2333
Size: 3744 2338
BlpI GC'TnA_GC
Cuts at: 3499 3499
Size: 6082
Bme1580I G_kGCm'C
Cuts at: 1790 2391 3103 4143 5299 1790
Size: 601 712 1040 1156 2573
Fragments arranged by size:
2573 1156 1040 712 601
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6082
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3813 4771 442
Size: 416 53 83 399 24 2396 958 1753
Fragments arranged by size:
2396 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6082
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3214 3214
Size: 6082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2632 4742 1239
Size: 1393 2110 2579
Fragments arranged by size:
2579 2110 1393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2405 3298 4207 5072 5370 2405
Size: 893 909 865 298 3117
Fragments arranged by size:
3117 909 893 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2281 4745 882
Size: 617 782 2464 2219
Fragments arranged by size:
2464 2219 782 617
BsaAI yAC'GTr
Cuts at: 493 1204 3873 493
Size: 711 2669 2702
Fragments arranged by size:
2702 2669 711
BsaBI GATnn'nnATC
Cuts at: 2664 6076 2664
Size: 3412 2670
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3889 4271 4906 275
Size: 53 83 186 936 2356 382 635 1451
Fragments arranged by size:
2356 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4515 4913 5187 5334 5745 4515
Size: 398 274 147 411 4852
Fragments arranged by size:
4852 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5857
Fragments arranged by size:
5857 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2478 1186
Size: 1292 4790
BseYI C'CCAG_C
Cuts at: 5285 5781 5285
Size: 496 5586
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5509
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3532 4293 5321 1540
Size: 275 186 1531 761 1028 2301
Fragments arranged by size:
2301 1531 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3103 3285 4143 4228 5299 3103
Size: 182 858 85 1071 3886
Fragments arranged by size:
3886 1071 858 182 85
BsmI GAATG_Cn'
Cuts at: 2197 3593 3686 2197
Size: 1396 93 4593
Fragments arranged by size:
4593 1396 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 3103 3285 3401 4143 4228 5299
Size: 399 601 712 182 116 742 85 1071
Cuts at: 5299 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 712 601 399 182 116
85
BspEI T'CCGG_A
Cuts at: 5745 5745
Size: 6082
BspHI T'CATG_A
Cuts at: 3001 3971 5701 3001
Size: 970 1730 3382
Fragments arranged by size:
3382 1730 970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3115 3152 3437 844
Size: 2271 37 285 3489
Fragments arranged by size:
3489 2271 285 37
BsrBI CCG'CTC
Cuts at: 1394 1823 3969 1394
Size: 429 2146 3507
Fragments arranged by size:
3507 2146 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4577 4751 65
Size: 1183 3329 174 1396
Fragments arranged by size:
3329 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4726 4913 1700
Size: 110 2916 187 2869
Fragments arranged by size:
2916 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6082
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6082
BssSI C'ACGA_G
Cuts at: 3165 3294 4142 5154 3165
Size: 129 848 1012 4093
Fragments arranged by size:
4093 1012 848 129
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3107 794
Size: 2313 3769
BstBI TT'CG_AA
Cuts at: 4886 4886
Size: 6082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6082
BstYI r'GATC_y
Cuts at: 1972 2333 3412 3779 4164 4181 5633 5719 5731
Size: 361 1079 367 385 17 1452 86 12
Cuts at: 5731 6077 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1079 385 367 361 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2350 2938 513
Size: 552 939 346 588 3657
Fragments arranged by size:
3657 939 588 552 346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5343
Fragments arranged by size:
5343 683 56
BtsI GCAGTG_nn'
Cuts at: 3598 4392 4412 3598
Size: 794 20 5268
Fragments arranged by size:
5268 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3303 3338 529
Size: 35 2739 35 3273
Fragments arranged by size:
3273 2739 35 35
DraI TTT'AAA
Cuts at: 3386 3386
Size: 6082
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5089 809
Size: 507 3773 1802
Fragments arranged by size:
3773 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3529 4418 6047
Size: 54 1425 50 275 18 1699 889 1629
Cuts at: 6047 8
Size: 43
Fragments arranged by size:
1699 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3529 1537
Size: 275 1717 4090
Fragments arranged by size:
4090 1717 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2560 4012 1353
Size: 1207 1452 3423
Fragments arranged by size:
3423 1452 1207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2299 3252 4669 5043 5189 387
Size: 1064 138 710 953 1417 374 146 1280
Fragments arranged by size:
1417 1280 1064 953 710 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2304 2632 3059 4145 4742 5529 5797
Size: 393 672 328 427 1086 597 787 268
Cuts at: 5797 1239
Size: 1524
Fragments arranged by size:
1524 1086 787 672 597 427 393 328
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3111 3111
Size: 6082
EcoO109I rG'GnC_Cy
Cuts at: 2483 2549 3019 3087 3116 2483
Size: 66 470 68 29 5449
Fragments arranged by size:
5449 470 68 66 29
EcoRI G'AATT_C
Cuts at: 3391 3769 3391
Size: 378 5704
FspI TGC'GCA
Cuts at: 4588 4588
Size: 6082
HaeII r_GCGC'y
Cuts at: 1678 5229 1678
Size: 3551 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 2605 2612 2637
Size: 32 135 32 541 32 460 7 25
Cuts at: 2637 2644 3338 3370 4723 4755 4797 4829 1373
Size: 7 694 32 1353 32 42 32 2626
Fragments arranged by size:
2626 1353 694 541 460 135 42 32
32 32 32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2631 2969 3426 3672 669
Size: 988 281 693 338 457 246 3079
Fragments arranged by size:
3079 988 693 457 338 281 246
HindIII A'AGCT_T
Cuts at: 748 2030 748
Size: 1282 4800
HpaI GTT'AAC
Cuts at: 3672 3672
Size: 6082
KpnI G_GTAC'C
Cuts at: 3407 3407
Size: 6082
MfeI C'AATT_G
Cuts at: 3681 3681
Size: 6082
MluI A'CGCG_T
Cuts at: 5958 5958
Size: 6082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2513 5196 5380 1305
Size: 1208 2683 184 2007
Fragments arranged by size:
2683 2007 1208 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4260
Fragments arranged by size:
4260 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2394 3048 3222 4040 4399 4558 518
Size: 648 1228 654 174 818 359 159 2042
Fragments arranged by size:
2042 1228 818 654 648 359 174 159
MspA1I CmG'CkG
Cuts at: 1606 3498 4175 5323 5568 1606
Size: 1892 677 1148 245 2120
Fragments arranged by size:
2120 1892 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2938 513
Size: 552 939 934 3657
Fragments arranged by size:
3657 939 934 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6082
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6082
NotI GC'GGCC_GC
Cuts at: 3529 3529
Size: 6082
NsiI A_TGCA'T
Cuts at: 2722 2833 2722
Size: 111 5971
NspI r_CATG'y
Cuts at: 1132 1229 2629 3239 3306 3440 4985 1132
Size: 97 1400 610 67 134 1545 2229
Fragments arranged by size:
2229 1545 1400 610 134 97 67
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6082
PciI A'CATG_T
Cuts at: 4981 4981
Size: 6082
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6082
PfoI T'CCnGG_A
Cuts at: 2609 2609
Size: 6082
PmeI GTTT'AAAC
Cuts at: 3386 3386
Size: 6082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4151 4183 5664 5696 4151
Size: 32 1481 32 4537
Fragments arranged by size:
4537 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2483 2549 3087 2483
Size: 66 538 5478
Fragments arranged by size:
5478 538 66
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6082
PsiI TTA'TAA
Cuts at: 3652 3652
Size: 6082
PspXI vC'TCGA_Gb
Cuts at: 3199 3370 3199
Size: 171 5911
PstI C_TGCA'G
Cuts at: 830 2182 3434 830
Size: 1352 1252 3478
Fragments arranged by size:
3478 1352 1252
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6082
SalI G'TCGA_C
Cuts at: 1936 2967 3424 1936
Size: 1031 457 4594
Fragments arranged by size:
4594 1031 457
SbfI CC_TGCA'GG
Cuts at: 3434 3434
Size: 6082
ScaI AGT'ACT
Cuts at: 4330 6042 4330
Size: 1712 4370
SfcI C'TryA_G
Cuts at: 826 1045 2178 3330 3430 4565 5246 5437 826
Size: 219 1133 1152 100 1135 681 191 1471
Fragments arranged by size:
1471 1152 1135 1133 681 219 191 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6082
SmaI CCC'GGG
Cuts at: 1872 3409 1872
Size: 1537 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 3199 3313 3370 4186 5087
Size: 197 926 477 779 114 57 816 901
Cuts at: 5087 5349 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 816 779 477 262 197
114 57
SnaBI TAC'GTA
Cuts at: 493 3873 493
Size: 3380 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6082
SphI G_CATG'C
Cuts at: 1132 3440 1132
Size: 2308 3774
SspI AAT'ATT
Cuts at: 5 52 4006 5
Size: 47 3954 2081
Fragments arranged by size:
3954 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6082
StyI C'CwwG_G
Cuts at: 513 1065 2004 2545 2938 3022 3521 513
Size: 552 939 541 393 84 499 3074
Fragments arranged by size:
3074 939 552 541 499 393 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4109 4126 4279 4464 4909 325
Size: 1255 2529 17 153 185 445 1498
Fragments arranged by size:
2529 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4328 6040 96
Size: 274 80 33 51 1443 2351 1712 138
Fragments arranged by size:
2351 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6042 6074 6042
Size: 32 6050
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6082
XbaI T'CTAG_A
Cuts at: 2708 2762 2819 2873 2930 2984 3418 2708
Size: 54 57 54 57 54 434 5372
Fragments arranged by size:
5372 434 57 57 54 54 54
XhoI C'TCGA_G
Cuts at: 1943 3199 3370 1943
Size: 1256 171 4655
Fragments arranged by size:
4655 1256 171
XmaI C'CCGG_G
Cuts at: 1870 3407 1870
Size: 1537 4545
XmnI GAAnn'nnTTC
Cuts at: 2317 4211 2317
Size: 1894 4188
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3890 276
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI