Restriction map for
pFN21AE5830
(Circular) (Six-base) MAPSORT of: pFN21AE5830.seq Check: 9352 from: 1 to: 7882
pFN21AE5830 7882 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3429 3597 3881 3429
Size: 168 284 7430
Fragments arranged by size:
7430 284 168
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5692 278
Size: 53 83 186 936 4156 2468
Fragments arranged by size:
4156 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5225 1937
Size: 3288 4594
Acc65I G'GTAC_C
Cuts at: 5203 5203
Size: 7882
AclI AA'CG_TT
Cuts at: 1563 6009 6382 1563
Size: 4446 373 3063
Fragments arranged by size:
4446 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5945 7329 7597 1632
Size: 4313 1384 268 1917
Fragments arranged by size:
4313 1917 1384 268
AfeI AGC'GCT
Cuts at: 2312 3482 4372 2312
Size: 1170 890 5822
Fragments arranged by size:
5822 1170 890
AflII C'TTAA_G
Cuts at: 820 1017 2419 820
Size: 197 1402 6283
Fragments arranged by size:
6283 1402 197
AflIII A'CryG_T
Cuts at: 1203 3651 5175 6781 7758 1203
Size: 2448 1524 1606 977 1327
Fragments arranged by size:
2448 1606 1524 1327 977
AgeI A'CCGG_T
Cuts at: 6713 6713
Size: 7882
AhdI GACnn_n'nnGTC
Cuts at: 6611 6611
Size: 7882
AleI CACnn'nnGTG
Cuts at: 1166 4754 1166
Size: 3588 4294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2591 1184
Size: 1407 6475
AlwNI CAG_nnn'CTG
Cuts at: 2084 3971 4340 4952 5005 5096 5125 5625 7197
Size: 1887 369 612 53 91 29 500 1572
Cuts at: 7197 2084
Size: 2769
Fragments arranged by size:
2769 1887 1572 612 500 369 91 53
29
ApaI G_GGCC'C
Cuts at: 2961 4018 4286 5039 2961
Size: 1057 268 753 5804
Fragments arranged by size:
5804 1057 753 268
ApaLI G'TGCA_C
Cuts at: 2342 2510 2594 3512 3764 5171 5939 7095 2342
Size: 168 84 918 252 1407 768 1156 3129
Fragments arranged by size:
3129 1407 1156 918 768 252 168 84
ApoI r'AATT_y
Cuts at: 1493 5191 5417 5569 7678 1493
Size: 3698 226 152 2109 1697
Fragments arranged by size:
3698 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6436 160
Size: 872 5404 1606
Fragments arranged by size:
5404 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7882
AvaI C'yCGr_G
Cuts at: 1870 1943 2698 2733 2954 4073 4102 4721 5032
Size: 73 755 35 221 1119 29 619 311
Cuts at: 5032 5051 5207 1870
Size: 19 156 4545
Fragments arranged by size:
4545 1119 755 619 311 221 156 73
35 29 19
BamHI G'GATC_C
Cuts at: 5212 5579 5212
Size: 367 7515
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2496 2684 2702 2778
Size: 325 217 627 254 455 188 18 76
Cuts at: 2778 2839 2968 2986 2998 3249 3304 3921 4660
Size: 61 129 18 12 251 55 617 739
Cuts at: 4660 4985 5203 6658 618
Size: 325 218 1455 1842
Fragments arranged by size:
1842 1455 739 627 617 455 325 325
254 251 218 217 188 129 76 61
55 18 18 12
BanII G_rGCy'C
Cuts at: 1391 2742 2850 2961 3117 3401 4018 4286 5039
Size: 1351 108 111 156 284 617 268 753
Cuts at: 5039 5049 5059 5201 1391
Size: 10 10 142 4072
Fragments arranged by size:
4072 1351 753 617 284 268 156 142
111 108 10 10
BbeI G_GCGC'C
Cuts at: 2045 2500 2688 2972 3002 3253 3308 2045
Size: 455 188 284 30 251 55 6619
Fragments arranged by size:
6619 455 284 251 188 55 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2743 928
Size: 971 844 6067
Fragments arranged by size:
6067 971 844
BbvCI CC'TCA_GC
Cuts at: 3267 4695 3267
Size: 1428 6454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2734 2768 3709 3743 6073 6107 1903
Size: 34 797 34 941 34 2330 34 3678
Fragments arranged by size:
3678 2330 941 797 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5774 6984 5774
Size: 1210 6672
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7882
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 2265 2379
Size: 107 122 71 631 605 430 163 114
Cuts at: 2379 2714 3549 6493 136
Size: 335 835 2944 1525
Fragments arranged by size:
2944 1525 835 631 605 430 335 163
122 114 107 71
BglII A'GATC_T
Cuts at: 7877 7877
Size: 7882
BlpI GC'TnA_GC
Cuts at: 4820 5299 4820
Size: 479 7403
Bme1580I G_kGCm'C
Cuts at: 1790 2204 2288 2346 2456 2514 2598 2954 2961
Size: 414 84 58 110 58 84 356 7
Cuts at: 2961 3516 3768 4018 4091 4286 5039 5175 5943
Size: 555 252 250 73 195 753 136 768
Cuts at: 5943 7099 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 768 753 555 414 356 252
250 195 136 110 84 84 73 58
58 7
BmgBI CAC'GTC
Cuts at: 1318 3654 4042 1318
Size: 2336 388 5158
Fragments arranged by size:
5158 2336 388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2101 2722 5613
Size: 416 53 83 399 24 684 621 2891
Cuts at: 5613 6571 442
Size: 958 1753
Fragments arranged by size:
2891 1753 958 684 621 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2768 2957 4040 4151 4231 4448 4490 4523
Size: 1529 189 1083 111 80 217 42 33
Cuts at: 4523 4556 4589 4769 4998 5043 6542 1239
Size: 33 33 180 229 45 1499 2579
Fragments arranged by size:
2579 1529 1499 1083 229 217 189 180
111 80 45 42 33 33 33
Bpu10I CC'TnA_GC
Cuts at: 2844 3267 4695 2844
Size: 423 1428 6031
Fragments arranged by size:
6031 1428 423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6007 6872 7170 6007
Size: 865 298 6719
Fragments arranged by size:
6719 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2861 6545 882
Size: 617 1362 3684 2219
Fragments arranged by size:
3684 2219 1362 617
BsaAI yAC'GTr
Cuts at: 493 1204 2509 3229 3679 5673 493
Size: 711 1305 720 450 1994 2702
Fragments arranged by size:
2702 1994 1305 720 711 450
BsaBI GATnn'nnATC
Cuts at: 7876 7876
Size: 7882
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2497 2685 2763
Size: 53 83 186 936 509 455 188 78
Cuts at: 2763 2969 2999 3250 3305 5689 6071 6706 275
Size: 206 30 251 55 2384 382 635 1451
Fragments arranged by size:
2384 1451 936 635 509 455 382 251
206 188 186 83 78 55 53 30
BsaWI w'CCGG_w
Cuts at: 2139 3099 6315 6713 6987 7134 7545 2139
Size: 960 3216 398 274 147 411 2476
Fragments arranged by size:
3216 2476 960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2870 2900 4861 4891 4908
Size: 30 165 30 1467 30 1961 30 17
Cuts at: 4908 4938 1178
Size: 30 4122
Fragments arranged by size:
4122 1961 1467 165 30 30 30 30
30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2221 2305 2641 2939 3033 3248 3391 3475
Size: 1035 84 336 298 94 215 143 84
Cuts at: 3475 3811 4234 4712 4916 4995 1186
Size: 336 423 478 204 79 4073
Fragments arranged by size:
4073 1035 478 423 336 336 298 215
204 143 94 84 84 79
BseYI C'CCAG_C
Cuts at: 2973 3110 3956 3984 4178 4253 4259 4373 4945
Size: 137 846 28 194 75 6 114 572
Cuts at: 4945 5026 5096 7085 7581 2973
Size: 81 70 1989 496 3274
Fragments arranged by size:
3274 1989 846 572 496 194 137 114
81 75 70 28 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 2651 3581 3821 4208 4376 4478
Size: 573 408 507 930 240 387 168 102
Cuts at: 4478 1163
Size: 4567
Fragments arranged by size:
4567 930 573 507 408 387 240 168
102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3246 3889 4270 5332 6093 7121
Size: 275 186 1245 643 381 1062 761 1028
Cuts at: 7121 1540
Size: 2301
Fragments arranged by size:
2301 1245 1062 1028 761 643 381 275
186
BsiHKAI G_wGCw'C
Cuts at: 2346 2514 2570 2598 2850 2943 3516 3768 5175
Size: 168 56 28 252 93 573 252 1407
Cuts at: 5175 5943 6028 7099 2346
Size: 768 85 1071 3129
Fragments arranged by size:
3129 1407 1071 768 573 252 252 168
93 85 56 28
BsmI GAATG_Cn'
Cuts at: 5393 5486 5393
Size: 93 7789
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2288 2346 2456 2514 2570 2598
Size: 399 414 84 58 110 58 56 28
Cuts at: 2598 2742 2850 2943 2954 2961 3117 3401 3516
Size: 144 108 93 11 7 156 284 115
Cuts at: 3516 3768 4018 4091 4286 5039 5049 5059 5175
Size: 252 250 73 195 753 10 10 116
Cuts at: 5175 5201 5943 6028 7099 1391
Size: 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 753 742 414 399 284 252
250 195 156 144 116 115 110 108
93 85 84 73 58 58 56 28
26 11 10 10 7
BspEI T'CCGG_A
Cuts at: 3099 7545 3099
Size: 4446 3436
BspHI T'CATG_A
Cuts at: 5771 7501 5771
Size: 1730 6152
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2583 2667 2778 3293 3429 3597 3753 3837
Size: 1739 84 111 515 136 168 156 84
Cuts at: 3837 3881 4677 5237 844
Size: 44 796 560 3489
Fragments arranged by size:
3489 1739 796 560 515 168 156 136
111 84 84 44
BsrBI CCG'CTC
Cuts at: 1394 1823 2188 2272 2524 2608 2626 3358 3442
Size: 429 365 84 252 84 18 732 84
Cuts at: 3442 3610 3778 3796 4364 4686 4824 5769 1394
Size: 168 168 18 568 322 138 945 3507
Fragments arranged by size:
3507 945 732 568 429 365 322 252
168 168 138 84 84 84 18 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 6377 6551 65
Size: 1183 5129 174 1396
Fragments arranged by size:
5129 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2348 2516 3146 3977 4616 4904
Size: 110 234 304 168 630 831 639 288
Cuts at: 4904 6526 6713 1700
Size: 1622 187 2869
Fragments arranged by size:
2869 1622 831 639 630 304 288 234
187 168 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7882
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7882
BssSI C'ACGA_G
Cuts at: 5942 6954 5942
Size: 1012 6870
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4810 794
Size: 4016 3866
BstBI TT'CG_AA
Cuts at: 6686 6686
Size: 7882
BstEII G'GTnAC_C
Cuts at: 1184 3280 4522 4588 1184
Size: 2096 1242 66 4478
Fragments arranged by size:
4478 2096 1242 66
BstXI CCAn_nnnn'nTGG
Cuts at: 3208 4672 3208
Size: 1464 6418
BstYI r'GATC_y
Cuts at: 1972 2328 5212 5579 5964 5981 7433 7519 7531
Size: 356 2884 367 385 17 1452 86 12
Cuts at: 7531 7877 1972
Size: 346 1977
Fragments arranged by size:
2884 1977 1452 385 367 356 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3926 5118 3926
Size: 1192 6690
BtgI C'CryG_G
Cuts at: 513 1065 2712 2835 2871 3136 3240 4286 4494
Size: 552 1647 123 36 265 104 1046 208
Cuts at: 4494 4560 4593 4611 5040 513
Size: 66 33 18 429 3355
Fragments arranged by size:
3355 1647 1046 552 429 265 208 123
104 66 36 33 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2910 487
Size: 683 56 1684 5459
Fragments arranged by size:
5459 1684 683 56
BtsI GCAGTG_nn'
Cuts at: 3227 4299 5398 6192 6212 3227
Size: 1072 1099 794 20 4897
Fragments arranged by size:
4897 1099 1072 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7882
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4618 4653 529
Size: 35 4054 35 3758
Fragments arranged by size:
4054 3758 35 35
DraI TTT'AAA
Cuts at: 5186 5186
Size: 7882
DraIII CAC_nnn'GTG
Cuts at: 3657 3657
Size: 7882
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6889 809
Size: 507 5573 1802
Fragments arranged by size:
5573 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2371 3198 3243
Size: 54 1425 50 275 18 541 827 45
Cuts at: 3243 3495 3541 3903 3942 4267 4365 4614 4618
Size: 252 46 362 39 325 98 249 4
Cuts at: 4618 5329 6218 7847 8
Size: 711 889 1629 43
Fragments arranged by size:
1629 1425 889 827 711 541 362 325
275 252 249 98 54 50 46 45
43 39 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3243 4267 5329 1537
Size: 275 1431 1024 1062 4090
Fragments arranged by size:
4090 1431 1062 1024 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2546 5812 1353
Size: 1193 3266 3423
Fragments arranged by size:
3423 3266 1193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2879 3328 3872 4194 6469 6843
Size: 1064 138 1290 449 544 322 2275 374
Cuts at: 6843 6989 387
Size: 146 1280
Fragments arranged by size:
2275 1290 1280 1064 544 449 374 322
146 138
EcoICRI GAG'CTC
Cuts at: 2848 2848
Size: 7882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2768 2957 4040 4151 4231 4448 4490
Size: 393 1136 189 1083 111 80 217 42
Cuts at: 4490 4523 4556 4589 4769 4998 5043 5945 6542
Size: 33 33 33 180 229 45 902 597
Cuts at: 6542 7329 7597 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1136 1083 902 787 597 393 268
229 217 189 180 111 80 45 42
33 33 33
EcoO109I rG'GnC_Cy
Cuts at: 2081 3696 4015 4099 4162 4282 4337 4718 5036
Size: 1615 319 84 63 120 55 381 318
Cuts at: 5036 5122 2081
Size: 86 4841
Fragments arranged by size:
4841 1615 381 319 318 120 86 84
63 55
EcoRI G'AATT_C
Cuts at: 5191 5569 5191
Size: 378 7504
FseI GG_CCGG'CC
Cuts at: 4620 4620
Size: 7882
FspI TGC'GCA
Cuts at: 6388 6388
Size: 7882
HaeII r_GCGC'y
Cuts at: 1678 2045 2314 2443 2500 2688 2972 3002 3253
Size: 367 269 129 57 188 284 30 251
Cuts at: 3253 3308 3484 3865 4124 4374 4944 7029 1678
Size: 55 176 381 259 250 570 2085 2531
Fragments arranged by size:
2531 2085 570 381 367 284 269 259
251 250 188 176 129 57 55 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4908 4940 6523 6555 6597
Size: 32 135 32 3336 32 1583 32 42
Cuts at: 6597 6629 1373
Size: 32 2626
Fragments arranged by size:
3336 2626 1583 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5226 5472 669
Size: 988 281 3288 246 3079
Fragments arranged by size:
3288 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7882
HpaI GTT'AAC
Cuts at: 5472 5472
Size: 7882
KasI G'GCGC_C
Cuts at: 2041 2496 2684 2968 2998 3249 3304 2041
Size: 455 188 284 30 251 55 6619
Fragments arranged by size:
6619 455 284 251 188 55 30
KpnI G_GTAC'C
Cuts at: 5207 5207
Size: 7882
MfeI C'AATT_G
Cuts at: 5481 5481
Size: 7882
MluI A'CGCG_T
Cuts at: 7758 7758
Size: 7882
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2679 3765 3849 4498 6996 7180 1305
Size: 1374 1086 84 649 2498 184 2007
Fragments arranged by size:
2498 2007 1374 1086 649 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3200 3497 3543 10
Size: 54 1425 343 1368 297 46 4349
Fragments arranged by size:
4349 1425 1368 343 297 54 46
MslI CAynn'nnrTG
Cuts at: 518 1166 2140 4754 5840 6199 6358 518
Size: 648 974 2614 1086 359 159 2042
Fragments arranged by size:
2614 2042 1086 974 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2619 2714 2776 2892 3165 3174 3196 3242
Size: 1013 95 62 116 273 9 22 46
Cuts at: 3242 3301 3508 3789 3919 4171 4288 4383 4694
Size: 59 207 281 130 252 117 95 311
Cuts at: 4694 5042 5167 5298 5975 7123 7368 1606
Size: 348 125 131 677 1148 245 2120
Fragments arranged by size:
2120 1148 1013 677 348 311 281 273
252 245 207 131 130 125 117 116
95 95 62 59 46 22 9
NaeI GCC'GGC
Cuts at: 2046 3148 3979 4618 2046
Size: 1102 831 639 5310
Fragments arranged by size:
5310 1102 831 639
NarI GG'CG_CC
Cuts at: 2042 2497 2685 2969 2999 3250 3305 2042
Size: 455 188 284 30 251 55 6619
Fragments arranged by size:
6619 455 284 251 188 55 30
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7330
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7882
NgoMIV G'CCGG_C
Cuts at: 2044 3146 3977 4616 2044
Size: 1102 831 639 5310
Fragments arranged by size:
5310 1102 831 639
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7882
NotI GC'GGCC_GC
Cuts at: 5329 5329
Size: 7882
NspI r_CATG'y
Cuts at: 1132 1229 5240 6785 1132
Size: 97 4011 1545 2229
Fragments arranged by size:
4011 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4159 4340 1348
Size: 2811 181 4890
Fragments arranged by size:
4890 2811 181
PciI A'CATG_T
Cuts at: 6781 6781
Size: 7882
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4340 4612 1108
Size: 3232 272 4378
Fragments arranged by size:
4378 3232 272
PfoI T'CCnGG_A
Cuts at: 2934 4761 2934
Size: 1827 6055
PmeI GTTT'AAAC
Cuts at: 5186 5186
Size: 7882
PmlI CAC'GTG
Cuts at: 2509 3229 3679 2509
Size: 720 450 6712
Fragments arranged by size:
6712 720 450
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5951 5983 7464 7496 5951
Size: 32 1481 32 6337
Fragments arranged by size:
6337 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4529 4595 1613
Size: 2916 66 4900
Fragments arranged by size:
4900 2916 66
PsiI TTA'TAA
Cuts at: 5452 5452
Size: 7882
PspOMI G'GGCC_C
Cuts at: 2957 4014 4282 5035 2957
Size: 1057 268 753 5804
Fragments arranged by size:
5804 1057 753 268
PspXI vC'TCGA_Gb
Cuts at: 4073 4073
Size: 7882
PstI C_TGCA'G
Cuts at: 830 2328 2775 3918 4413 5001 5234 830
Size: 1498 447 1143 495 588 233 3478
Fragments arranged by size:
3478 1498 1143 588 495 447 233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7882
PvuII CAG'CTG
Cuts at: 3174 3196 4171 4694 5167 3174
Size: 22 975 523 473 5889
Fragments arranged by size:
5889 975 523 473 22
SacI G_AGCT'C
Cuts at: 2850 2850
Size: 7882
SacII CC_GC'GG
Cuts at: 2715 3243 4289 5043 2715
Size: 528 1046 754 5554
Fragments arranged by size:
5554 1046 754 528
SalI G'TCGA_C
Cuts at: 1936 5224 1936
Size: 3288 4594
SapI GCTCTTCn'nnn_
Cuts at: 2546 2546
Size: 7882
SbfI CC_TGCA'GG
Cuts at: 5234 5234
Size: 7882
ScaI AGT'ACT
Cuts at: 6130 7842 6130
Size: 1712 6170
SfcI C'TryA_G
Cuts at: 826 1045 2324 2771 3914 4409 4997 5230 6365
Size: 219 1279 447 1143 495 588 233 1135
Cuts at: 6365 7046 7237 826
Size: 681 191 1471
Fragments arranged by size:
1471 1279 1143 1135 681 588 495 447
233 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2379 3549 2379
Size: 1170 6712
SfoI GGC'GCC
Cuts at: 2043 2498 2686 2970 3000 3251 3306 2043
Size: 455 188 284 30 251 55 6619
Fragments arranged by size:
6619 455 284 251 188 55 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7882
SgrAI Cr'CCGG_yG
Cuts at: 1700 2516 1700
Size: 816 7066
SmaI CCC'GGG
Cuts at: 1872 2700 2735 2956 4723 5053 5209 1872
Size: 828 35 221 1767 330 156 4545
Fragments arranged by size:
4545 1767 828 330 221 156 35
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2419 4073 5986 6887 7149 820
Size: 197 926 476 1654 1913 901 262 1553
Fragments arranged by size:
1913 1654 1553 926 901 476 262 197
SnaBI TAC'GTA
Cuts at: 493 5673 493
Size: 5180 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7882
SphI G_CATG'C
Cuts at: 1132 5240 1132
Size: 4108 3774
SspI AAT'ATT
Cuts at: 5 52 5806 5
Size: 47 5754 2081
Fragments arranged by size:
5754 2081 47
StuI AGG'CCT
Cuts at: 1517 2303 2471 3473 3641 1517
Size: 786 168 1002 168 5758
Fragments arranged by size:
5758 1002 786 168 168
StyI C'CwwG_G
Cuts at: 513 1065 2214 2298 2466 3003 5321 513
Size: 552 1149 84 168 537 2318 3074
Fragments arranged by size:
3074 2318 1149 552 537 168 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2660 3830 4043 4678 5909 5926 6079
Size: 1255 1080 1170 213 635 1231 17 153
Cuts at: 6079 6264 6709 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1231 1170 1080 635 445 213
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6128 7840 96
Size: 274 80 33 51 1443 4151 1712 138
Fragments arranged by size:
4151 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7842 7874 7842
Size: 32 7850
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7882
XbaI T'CTAG_A
Cuts at: 5218 5218
Size: 7882
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2114 2878 3683 4753 4957 2114
Size: 764 805 1070 204 5039
Fragments arranged by size:
5039 1070 805 764 204
XhoI C'TCGA_G
Cuts at: 1943 4073 1943
Size: 2130 5752
XmaI C'CCGG_G
Cuts at: 1870 2698 2733 2954 4721 5051 5207 1870
Size: 828 35 221 1767 330 156 4545
Fragments arranged by size:
4545 1767 828 330 221 156 35
XmnI GAAnn'nnTTC
Cuts at: 2555 4872 6011 2555
Size: 2317 1139 4426
Fragments arranged by size:
4426 2317 1139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5690 276
Size: 53 83 186 936 4156 2468
Fragments arranged by size:
4156 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoNI EcoRV FalI FspAI NruI NsiI PacI
PpuMI PsrI RsrII SanDI SexAI SrfI SwaI