Restriction map for
pFN21AE5819
(Circular) (Six-base) MAPSORT of: pFN21AE5819.seq Check: 4362 from: 1 to: 7894
pFN21AE5819 7894 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5704 278
Size: 53 83 186 936 4168 2468
Fragments arranged by size:
4168 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5237 1937
Size: 3300 4594
Acc65I G'GTAC_C
Cuts at: 3799 5215 3799
Size: 1416 6478
AclI AA'CG_TT
Cuts at: 1563 4452 6021 6394 1563
Size: 2889 1569 373 3063
Fragments arranged by size:
3063 2889 1569 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2727 2997 3033 3601 4211 4764 5957
Size: 557 538 270 36 568 610 553 1193
Cuts at: 5957 7341 7609 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1193 610 568 557 553 538
270 268 36
AflII C'TTAA_G
Cuts at: 820 1017 4589 820
Size: 197 3572 4125
Fragments arranged by size:
4125 3572 197
AflIII A'CryG_T
Cuts at: 1203 3793 6793 7770 1203
Size: 2590 3000 977 1327
Fragments arranged by size:
3000 2590 1327 977
AgeI A'CCGG_T
Cuts at: 2666 6725 2666
Size: 4059 3835
AhdI GACnn_n'nnGTC
Cuts at: 4545 6623 4545
Size: 2078 5816
AleI CACnn'nnGTG
Cuts at: 1166 3727 5071 1166
Size: 2561 1344 3989
Fragments arranged by size:
3989 2561 1344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4149 1184
Size: 2965 4929
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2102 2134 5024 5056 2102
Size: 32 2890 32 4940
Fragments arranged by size:
4940 2890 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2105 2167 2355 3056 5637 7209 2105
Size: 62 188 701 2581 1572 2790
Fragments arranged by size:
2790 2581 1572 701 188 62
ApaI G_GGCC'C
Cuts at: 2186 2186
Size: 7894
ApaLI G'TGCA_C
Cuts at: 5951 7107 5951
Size: 1156 6738
ApoI r'AATT_y
Cuts at: 1493 3224 5203 5429 5581 7690 1493
Size: 1731 1979 226 152 2109 1697
Fragments arranged by size:
2109 1979 1731 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6448 160
Size: 872 5416 1606
Fragments arranged by size:
5416 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7894
AvaI C'yCGr_G
Cuts at: 1870 1943 2197 2225 2455 2762 3127 4511 5219
Size: 73 254 28 230 307 365 1384 708
Cuts at: 5219 1870
Size: 4545
Fragments arranged by size:
4545 1384 708 365 307 254 230 73
28
BamHI G'GATC_C
Cuts at: 5224 5591 5224
Size: 367 7527
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3799 4082 4784 5155 5215
Size: 325 217 627 2012 283 702 371 60
Cuts at: 5215 6670 618
Size: 1455 1842
Fragments arranged by size:
2012 1842 1455 702 627 371 325 283
217 60
BanII G_rGCy'C
Cuts at: 1391 2186 2204 2235 2727 2763 3128 3470 5018
Size: 795 18 31 492 36 365 342 1548
Cuts at: 5018 5066 5213 1391
Size: 48 147 4072
Fragments arranged by size:
4072 1548 795 492 365 342 147 48
36 31 18
BbeI G_GCGC'C
Cuts at: 4086 4086
Size: 7894
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2885 3021 3196 4702 928
Size: 971 986 136 175 1506 4120
Fragments arranged by size:
4120 1506 986 971 175 136
BbvCI CC'TCA_GC
Cuts at: 2803 2803
Size: 7894
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2951 2985 4331 4365 6085 6119 1903
Size: 34 1014 34 1346 34 1720 34 3678
Fragments arranged by size:
3678 1720 1346 1014 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3999 5786 6996 3999
Size: 1787 1210 4897
Fragments arranged by size:
4897 1787 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7894
BfrBI ATG'CAT
Cuts at: 4053 4998 4053
Size: 945 6949
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2436 2813 6505
Size: 107 122 71 631 605 764 377 3692
Cuts at: 6505 136
Size: 1525
Fragments arranged by size:
3692 1525 764 631 605 377 122 107
71
BglII A'GATC_T
Cuts at: 7889 7889
Size: 7894
BlpI GC'TnA_GC
Cuts at: 2832 3122 4256 5017 5311 2832
Size: 290 1134 761 294 5415
Fragments arranged by size:
5415 1134 761 294 290
Bme1580I G_kGCm'C
Cuts at: 1790 2186 2462 5158 5955 7111 1790
Size: 396 276 2696 797 1156 2573
Fragments arranged by size:
2696 2573 1156 797 396 276
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7894
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3661 3842 5625
Size: 416 53 83 399 24 2244 181 1783
Cuts at: 5625 6583 442
Size: 958 1753
Fragments arranged by size:
2244 1783 1753 958 416 399 181 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7894
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2097 4195 2097
Size: 2098 5796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2498 3509 4159 4190 4840 6554 1239
Size: 1259 1011 650 31 650 1714 2579
Fragments arranged by size:
2579 1714 1259 1011 650 650 31
Bpu10I CC'TnA_GC
Cuts at: 2803 3469 3989 5093 2803
Size: 666 520 1104 5604
Fragments arranged by size:
5604 1104 666 520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3005 4430 6019 6884 7182 3005
Size: 1425 1589 865 298 3717
Fragments arranged by size:
3717 1589 1425 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2939 5123 6557 882
Size: 617 1440 2184 1434 2219
Fragments arranged by size:
2219 2184 1440 1434 617
BsaAI yAC'GTr
Cuts at: 493 1204 3796 5685 493
Size: 711 2592 1889 2702
Fragments arranged by size:
2702 2592 1889 711
BsaBI GATnn'nnATC
Cuts at: 7888 7888
Size: 7894
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2129 4083 5701 6083
Size: 53 83 186 936 596 1954 1618 382
Cuts at: 6083 6718 275
Size: 635 1451
Fragments arranged by size:
1954 1618 1451 936 635 596 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2666 4278 6327 6725 6999 7146 7557 2666
Size: 1612 2049 398 274 147 411 3003
Fragments arranged by size:
3003 2049 1612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2102 2132 2477 2507 2714
Size: 30 165 30 699 30 345 30 207
Cuts at: 2714 2744 2899 2929 3129 3159 4508 4538 5024
Size: 30 155 30 200 30 1349 30 486
Cuts at: 5024 5054 1178
Size: 30 4018
Fragments arranged by size:
4018 1349 699 486 345 207 200 165
155 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 2904 3150 3228 3231 3868 4529 5032
Size: 1057 661 246 78 3 637 661 503
Cuts at: 5032 5116 5152 1186
Size: 84 36 3928
Fragments arranged by size:
3928 1057 661 661 637 503 246 84
78 36 3
BseYI C'CCAG_C
Cuts at: 2418 4078 4876 4947 5088 5113 7097 7593 2418
Size: 1660 798 71 141 25 1984 496 2719
Fragments arranged by size:
2719 1984 1660 798 496 141 71 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3069 3248 4082 1163
Size: 573 1333 179 834 4975
Fragments arranged by size:
4975 1333 834 573 179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5344 6105 7133 1540
Size: 275 186 3343 761 1028 2301
Fragments arranged by size:
3343 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2727 4344 5018 5955 6040 7111 2727
Size: 1617 674 937 85 1071 3510
Fragments arranged by size:
3510 1617 1071 937 674 85
BsmI GAATG_Cn'
Cuts at: 5405 5498 5405
Size: 93 7801
BsmBI CGTCTCn'nnnn_
Cuts at: 2957 2957
Size: 7894
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2204 2235 2462 2727 2763 3128
Size: 399 396 18 31 227 265 36 365
Cuts at: 3128 3470 4344 5018 5066 5158 5213 5955 6040
Size: 342 874 674 48 92 55 742 85
Cuts at: 6040 7111 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 874 742 674 399 396 365
342 265 227 92 85 55 48 36
31 18
BspEI T'CCGG_A
Cuts at: 7557 7557
Size: 7894
BspHI T'CATG_A
Cuts at: 3286 3534 5783 7513 3286
Size: 248 2249 1730 3667
Fragments arranged by size:
3667 2249 1730 248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5249 844
Size: 4405 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4166 5781 1394
Size: 429 2343 1615 3507
Fragments arranged by size:
3507 2343 1615 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3803 5185 6389 6563 65
Size: 1183 2555 1382 1204 174 1396
Fragments arranged by size:
2555 1396 1382 1204 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2666 4395 6538 6725 1700
Size: 110 856 1729 2143 187 2869
Fragments arranged by size:
2869 2143 1729 856 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7894
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7894
BssSI C'ACGA_G
Cuts at: 5954 6966 5954
Size: 1012 6882
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2064 2822 3315 4252 4944 794
Size: 1270 758 493 937 692 3744
Fragments arranged by size:
3744 1270 937 758 692 493
BstBI TT'CG_AA
Cuts at: 6698 6698
Size: 7894
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7894
BstXI CCAn_nnnn'nTGG
Cuts at: 2426 2476 3280 4001 2426
Size: 50 804 721 6319
Fragments arranged by size:
6319 804 721 50
BstYI r'GATC_y
Cuts at: 1972 5224 5591 5976 5993 7445 7531 7543 7889
Size: 3252 367 385 17 1452 86 12 346
Cuts at: 7889 1972
Size: 1977
Fragments arranged by size:
3252 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2388 4149 2388
Size: 1761 6133
BtgI C'CryG_G
Cuts at: 513 1065 2004 2381 4121 4848 513
Size: 552 939 377 1740 727 3559
Fragments arranged by size:
3559 1740 939 727 552 377
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7155
Fragments arranged by size:
7155 683 56
BtsI GCAGTG_nn'
Cuts at: 2929 4099 4926 5410 6204 6224 2929
Size: 1170 827 484 794 20 4599
Fragments arranged by size:
4599 1170 827 794 484 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7894
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2491 2526 529
Size: 35 1927 35 5897
Fragments arranged by size:
5897 1927 35 35
DraI TTT'AAA
Cuts at: 5198 5198
Size: 7894
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6901 809
Size: 507 5585 1802
Fragments arranged by size:
5585 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2437 2556 4977
Size: 54 1425 50 275 18 607 119 2421
Cuts at: 4977 5341 6230 7859 8
Size: 364 889 1629 43
Fragments arranged by size:
2421 1629 1425 889 607 364 275 119
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5341 1537
Size: 275 3529 4090
Fragments arranged by size:
4090 3529 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3174 3758 4280 5824 1353
Size: 1821 584 522 1544 3423
Fragments arranged by size:
3423 1821 1544 584 522
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6481 6855 7001 387
Size: 1064 138 4892 374 146 1280
Fragments arranged by size:
4892 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2725 5016 2725
Size: 2291 5603
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2498 2727 2997 3033 3509 3601
Size: 393 557 309 229 270 36 476 92
Cuts at: 3601 4159 4190 4211 4764 4840 5957 6554 7341
Size: 558 31 21 553 76 1117 597 787
Cuts at: 7341 7609 1239
Size: 268 1524
Fragments arranged by size:
1524 1117 787 597 558 557 553 476
393 309 270 268 229 92 76 36
31 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2654 3572 5171 2654
Size: 918 1599 5377
Fragments arranged by size:
5377 1599 918
EcoO109I rG'GnC_Cy
Cuts at: 2182 2799 3565 4719 2182
Size: 617 766 1154 5357
Fragments arranged by size:
5357 1154 766 617
EcoRI G'AATT_C
Cuts at: 5203 5581 5203
Size: 378 7516
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4739 4739
Size: 7894
FspI TGC'GCA
Cuts at: 6400 6400
Size: 7894
HaeII r_GCGC'y
Cuts at: 1678 4086 5187 7041 1678
Size: 2408 1101 1854 2531
Fragments arranged by size:
2531 2408 1854 1101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 3947 3979 4508
Size: 32 135 32 905 32 1438 32 529
Cuts at: 4508 4531 4540 4563 5048 5080 6535 6567 6609
Size: 23 9 23 485 32 1455 32 42
Cuts at: 6609 6641 1373
Size: 32 2626
Fragments arranged by size:
2626 1455 1438 905 529 485 135 42
32 32 32 32 32 32 32 23
23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3433 5238 5484 669
Size: 988 281 1495 1805 246 3079
Fragments arranged by size:
3079 1805 1495 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7894
HpaI GTT'AAC
Cuts at: 5484 5484
Size: 7894
KasI G'GCGC_C
Cuts at: 4082 4082
Size: 7894
KpnI G_GTAC'C
Cuts at: 3803 5219 3803
Size: 1416 6478
MfeI C'AATT_G
Cuts at: 5493 5493
Size: 7894
MluI A'CGCG_T
Cuts at: 7770 7770
Size: 7894
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7008 7192 1305
Size: 5703 184 2007
Fragments arranged by size:
5703 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2439 2558 4979 10
Size: 54 1425 343 607 119 2421 2925
Fragments arranged by size:
2925 2421 1425 607 343 119 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3727 4131 5071 5852 6211 6370 518
Size: 648 2561 404 940 781 359 159 2042
Fragments arranged by size:
2561 2042 940 781 648 404 359 159
MspA1I CmG'CkG
Cuts at: 1606 3053 3438 3593 3727 4123 4850 5071 5113
Size: 1447 385 155 134 396 727 221 42
Cuts at: 5113 5310 5987 7135 7380 1606
Size: 197 677 1148 245 2120
Fragments arranged by size:
2120 1447 1148 727 677 396 385 245
221 197 155 134 42
NarI GG'CG_CC
Cuts at: 4083 4083
Size: 7894
NcoI C'CATG_G
Cuts at: 513 1065 2004 2381 513
Size: 552 939 377 6026
Fragments arranged by size:
6026 939 552 377
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7894
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7894
NotI GC'GGCC_GC
Cuts at: 5341 5341
Size: 7894
NsiI A_TGCA'T
Cuts at: 4055 5000 4055
Size: 945 6949
NspI r_CATG'y
Cuts at: 1132 1229 2030 4771 5252 6797 1132
Size: 97 801 2741 481 1545 2229
Fragments arranged by size:
2741 2229 1545 801 481 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7894
PciI A'CATG_T
Cuts at: 6793 6793
Size: 7894
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2539 1108
Size: 1431 6463
PfoI T'CCnGG_A
Cuts at: 2249 2592 3099 4194 4368 4402 4485 2249
Size: 343 507 1095 174 34 83 5658
Fragments arranged by size:
5658 1095 507 343 174 83 34
PmeI GTTT'AAAC
Cuts at: 5198 5198
Size: 7894
PmlI CAC'GTG
Cuts at: 3796 3796
Size: 7894
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2102 2134 3377 3409 5024 5056 5963 5995 7476
Size: 32 1243 32 1615 32 907 32 1481
Cuts at: 7476 7508 2102
Size: 32 2488
Fragments arranged by size:
2488 1615 1481 1243 907 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 3565 4719 3565
Size: 1154 6740
PshAI GACnn'nnGTC
Cuts at: 1613 3282 1613
Size: 1669 6225
PsiI TTA'TAA
Cuts at: 3369 5464 3369
Size: 2095 5799
PspOMI G'GGCC_C
Cuts at: 2182 2182
Size: 7894
PstI C_TGCA'G
Cuts at: 830 2060 2089 2529 2778 3630 4574 4634 4669
Size: 1230 29 440 249 852 944 60 35
Cuts at: 4669 5246 830
Size: 577 3478
Fragments arranged by size:
3478 1230 944 852 577 440 249 60
35 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7894
PvuII CAG'CTG
Cuts at: 3053 3727 5071 3053
Size: 674 1344 5876
Fragments arranged by size:
5876 1344 674
SacI G_AGCT'C
Cuts at: 2727 5018 2727
Size: 2291 5603
SacII CC_GC'GG
Cuts at: 4124 4851 4124
Size: 727 7167
SalI G'TCGA_C
Cuts at: 1936 5236 1936
Size: 3300 4594
SanDI GG'GwC_CC
Cuts at: 4719 4719
Size: 7894
SbfI CC_TGCA'GG
Cuts at: 2529 5246 2529
Size: 2717 5177
ScaI AGT'ACT
Cuts at: 3488 6142 7854 3488
Size: 2654 1712 3528
Fragments arranged by size:
3528 2654 1712
SexAI A'CCwGG_T
Cuts at: 3567 4029 4311 3567
Size: 462 282 7150
Fragments arranged by size:
7150 462 282
SfcI C'TryA_G
Cuts at: 826 1045 2056 2085 2525 2774 2826 3626 4570
Size: 219 1011 29 440 249 52 800 944
Cuts at: 4570 4630 4665 5242 6377 7058 7249 826
Size: 60 35 577 1135 681 191 1471
Fragments arranged by size:
1471 1135 1011 944 800 681 577 440
249 219 191 60 52 35 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 2436 2436
Size: 7894
SfoI GGC'GCC
Cuts at: 4084 4084
Size: 7894
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7894
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7894
SmaI CCC'GGG
Cuts at: 1872 2199 2457 2764 3129 5221 1872
Size: 327 258 307 365 2092 4545
Fragments arranged by size:
4545 2092 365 327 307 258
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3020 4409 4589 5998 6899 7161
Size: 197 926 1077 1389 180 1409 901 262
Cuts at: 7161 820
Size: 1553
Fragments arranged by size:
1553 1409 1389 1077 926 901 262 197
180
SnaBI TAC'GTA
Cuts at: 493 5685 493
Size: 5192 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7894
SphI G_CATG'C
Cuts at: 1132 5252 1132
Size: 4120 3774
SspI AAT'ATT
Cuts at: 5 52 5818 5
Size: 47 5766 2081
Fragments arranged by size:
5766 2081 47
StuI AGG'CCT
Cuts at: 1517 2649 3873 4520 1517
Size: 1132 1224 647 4891
Fragments arranged by size:
4891 1224 1132 647
StyI C'CwwG_G
Cuts at: 513 1065 2004 2034 2381 2955 4000 4714 5172
Size: 552 939 30 347 574 1045 714 458
Cuts at: 5172 5333 513
Size: 161 3074
Fragments arranged by size:
3074 1045 939 714 574 552 458 347
161 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3680 3749 5921 5938 6091 6276 6721
Size: 1255 2100 69 2172 17 153 185 445
Cuts at: 6721 325
Size: 1498
Fragments arranged by size:
2172 2100 1498 1255 445 185 153 69
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3486 3744 6140
Size: 274 80 33 51 1443 1509 258 2396
Cuts at: 6140 7852 96
Size: 1712 138
Fragments arranged by size:
2396 1712 1509 1443 274 258 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3129 3161 3920 3952 7854 7886 3129
Size: 32 759 32 3902 32 3137
Fragments arranged by size:
3902 3137 759 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7894
XbaI T'CTAG_A
Cuts at: 5230 5230
Size: 7894
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2473 2473
Size: 7894
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7894
XmaI C'CCGG_G
Cuts at: 1870 2197 2455 2762 3127 5219 1870
Size: 327 258 307 365 2092 4545
Fragments arranged by size:
4545 2092 365 327 307 258
XmnI GAAnn'nnTTC
Cuts at: 3656 4693 6023 3656
Size: 1037 1330 5527
Fragments arranged by size:
5527 1330 1037
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5702 276
Size: 53 83 186 936 4168 2468
Fragments arranged by size:
4168 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BsiWI BstZ17I DraIII
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspXI
PsrI RsrII SapI SrfI SwaI