Restriction map for
pFN21AE5812
(Circular) (Six-base) MAPSORT of: pFN21AE5812.seq Check: 8560 from: 1 to: 8314
pFN21AE5812 8314 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2575 2575
Size: 8314
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5439 6124 278
Size: 53 83 186 936 3903 685 2468
Fragments arranged by size:
3903 2468 936 685 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5657 1937
Size: 3720 4594
Acc65I G'GTAC_C
Cuts at: 4206 5635 4206
Size: 1429 6885
AclI AA'CG_TT
Cuts at: 1563 6441 6814 1563
Size: 4878 373 3063
Fragments arranged by size:
4878 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3046 4159 6377 7761 8029 1632
Size: 1414 1113 2218 1384 268 1917
Fragments arranged by size:
2218 1917 1414 1384 1113 268
AfeI AGC'GCT
Cuts at: 2705 2705
Size: 8314
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8117
AflIII A'CryG_T
Cuts at: 1203 5334 7213 8190 1203
Size: 4131 1879 977 1327
Fragments arranged by size:
4131 1879 1327 977
AgeI A'CCGG_T
Cuts at: 7145 7145
Size: 8314
AhdI GACnn_n'nnGTC
Cuts at: 2608 3679 7043 2608
Size: 1071 3364 3879
Fragments arranged by size:
3879 3364 1071
AleI CACnn'nnGTG
Cuts at: 1166 3254 4401 1166
Size: 2088 1147 5079
Fragments arranged by size:
5079 2088 1147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5129 1184
Size: 3945 4369
AlwNI CAG_nnn'CTG
Cuts at: 2374 3069 3616 3945 4243 4672 5051 6057 7629
Size: 695 547 329 298 429 379 1006 1572
Cuts at: 7629 2374
Size: 3059
Fragments arranged by size:
3059 1572 1006 695 547 429 379 329
298
ApaI G_GGCC'C
Cuts at: 2096 2128 2202 2283 3598 3928 4102 4913 2096
Size: 32 74 81 1315 330 174 811 5497
Fragments arranged by size:
5497 1315 811 330 174 81 74 32
ApaLI G'TGCA_C
Cuts at: 4262 4561 5303 6371 7527 4262
Size: 299 742 1068 1156 5049
Fragments arranged by size:
5049 1156 1068 742 299
ApoI r'AATT_y
Cuts at: 1493 5623 5849 6001 8110 1493
Size: 4130 226 152 2109 1697
Fragments arranged by size:
4130 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6868 160
Size: 872 5836 1606
Fragments arranged by size:
5836 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8314
AvaI C'yCGr_G
Cuts at: 1870 1943 2167 2385 2598 2924 3361 3648 3966
Size: 73 224 218 213 326 437 287 318
Cuts at: 3966 4164 5238 5321 5639 1870
Size: 198 1074 83 318 4545
Fragments arranged by size:
4545 1074 437 326 318 318 287 224
218 213 198 83 73
BamHI G'GATC_C
Cuts at: 2565 3736 4690 5644 6011 2565
Size: 1171 954 954 367 4868
Fragments arranged by size:
4868 1171 954 954 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2486 2941 3464 3932 4206
Size: 325 217 627 699 455 523 468 274
Cuts at: 4206 4468 4837 5635 7090 618
Size: 262 369 798 1455 1842
Fragments arranged by size:
1842 1455 798 699 627 523 468 455
369 325 274 262 217
BanII G_rGCy'C
Cuts at: 1391 2096 2118 2128 2174 2202 2283 2309 2823
Size: 705 22 10 46 28 81 26 514
Cuts at: 2823 2901 3598 3928 3960 4102 4482 4656 4913
Size: 78 697 330 32 142 380 174 257
Cuts at: 4913 4993 5490 5633 1391
Size: 80 497 143 4072
Fragments arranged by size:
4072 705 697 514 497 380 330 257
174 143 142 81 80 78 46 32
28 26 22 10
BbeI G_GCGC'C
Cuts at: 4472 4472
Size: 8314
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3491 5481 928
Size: 971 1592 1990 3761
Fragments arranged by size:
3761 1990 1592 971
BbvCI CC'TCA_GC
Cuts at: 2255 4617 4682 2255
Size: 2362 65 5887
Fragments arranged by size:
5887 2362 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3070 3104 6505 6539 1903
Size: 34 1133 34 3401 34 3678
Fragments arranged by size:
3678 3401 1133 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3154 6206 7416 3154
Size: 3052 1210 4052
Fragments arranged by size:
4052 3052 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2105 2474 3005
Size: 107 122 71 631 605 433 369 531
Cuts at: 3005 3275 4115 4420 4518 6925 136
Size: 270 840 305 98 2407 1525
Fragments arranged by size:
2407 1525 840 631 605 531 433 369
305 270 122 107 98 71
BglII A'GATC_T
Cuts at: 3516 8309 3516
Size: 4793 3521
BlpI GC'TnA_GC
Cuts at: 2526 4214 4445 4634 4943 5731 2526
Size: 1688 231 189 309 788 5109
Fragments arranged by size:
5109 1688 788 309 231 189
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2128 2202 2283 2946 3354 3467 3539
Size: 306 32 74 81 663 408 113 72
Cuts at: 3539 3598 3928 3935 4102 4266 4565 4913 4974
Size: 59 330 7 167 164 299 348 61
Cuts at: 4974 5307 5574 6375 7531 1790
Size: 333 267 801 1156 2573
Fragments arranged by size:
2573 1156 801 663 408 348 333 330
306 299 267 167 164 113 81 74
72 61 59 32 7
BmgBI CAC'GTC
Cuts at: 1318 2866 3676 1318
Size: 1548 810 5956
Fragments arranged by size:
5956 1548 810
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2233 3346 4034
Size: 416 53 83 399 24 816 1113 688
Cuts at: 4034 6045 7003 442
Size: 2011 958 1753
Fragments arranged by size:
2011 1753 1113 958 816 688 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3242 1056
Size: 2186 6128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2969 2984 3062 3152 3317 4037 4520 4694
Size: 1730 15 78 90 165 720 483 174
Cuts at: 4694 4760 4769 4793 6974 1239
Size: 66 9 24 2181 2579
Fragments arranged by size:
2579 2181 1730 720 483 174 165 90
78 66 24 15 9
Bpu10I CC'TnA_GC
Cuts at: 2112 2255 2364 2498 4617 4682 2112
Size: 143 109 134 2119 65 5744
Fragments arranged by size:
5744 2119 143 134 109 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2957 4937 6439 7304 7602 2957
Size: 1980 1502 865 298 3669
Fragments arranged by size:
3669 1980 1502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3578 4121 4956 6977 882
Size: 617 2079 543 835 2021 2219
Fragments arranged by size:
2219 2079 2021 835 617 543
BsaAI yAC'GTr
Cuts at: 493 1204 4261 4938 5266 6105 493
Size: 711 3057 677 328 839 2702
Fragments arranged by size:
3057 2702 839 711 677 328
BsaBI GATnn'nnATC
Cuts at: 8308 8308
Size: 8314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4469 5436 6121 6503
Size: 53 83 186 936 2936 967 685 382
Cuts at: 6503 7138 275
Size: 635 1451
Fragments arranged by size:
2936 1451 967 936 685 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3630 4358 6747 7145 7419 7566 7977 3630
Size: 728 2389 398 274 147 411 3967
Fragments arranged by size:
3967 2389 728 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 3785 3815 3836
Size: 30 165 30 993 30 1359 30 21
Cuts at: 3836 3866 4586 4616 4700 4730 4953 4983 5540
Size: 30 720 30 84 30 223 30 557
Cuts at: 5540 5570 1178
Size: 30 3922
Fragments arranged by size:
3922 1359 993 720 557 223 165 84
30 30 30 30 30 30 30 30
30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2437 2733 4094 4721 4778 1186
Size: 1251 296 1361 627 57 4722
Fragments arranged by size:
4722 1361 1251 627 296 57
BseYI C'CCAG_C
Cuts at: 2059 2065 2095 2117 2128 2184 2797 3616 4048
Size: 6 30 22 11 56 613 819 432
Cuts at: 4048 4095 4533 4610 4794 4841 4906 5018 5196
Size: 47 438 77 184 47 65 112 178
Cuts at: 5196 7517 8013 2059
Size: 2321 496 2360
Fragments arranged by size:
2360 2321 819 613 496 438 432 184
178 112 77 65 56 47 47 30
22 11 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2269 2598 3209 3939 4901 5207 5288
Size: 573 533 329 611 730 962 306 81
Cuts at: 5288 5405 1163
Size: 117 4072
Fragments arranged by size:
4072 962 730 611 573 533 329 306
117 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2857 3592 3812 5764 6525 7553
Size: 275 186 856 735 220 1952 761 1028
Cuts at: 7553 1540
Size: 2301
Fragments arranged by size:
2301 1952 1028 856 761 735 275 220
186
BsiHKAI G_wGCw'C
Cuts at: 2174 2823 2955 3083 4266 4565 5307 6375 6460
Size: 649 132 128 1183 299 742 1068 85
Cuts at: 6460 7531 2174
Size: 1071 2957
Fragments arranged by size:
2957 1183 1071 1068 742 649 299 132
128 85
BsmI GAATG_Cn'
Cuts at: 2826 5825 5918 2826
Size: 2999 93 5222
Fragments arranged by size:
5222 2999 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3689 3689
Size: 8314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2118 2128 2174 2202 2283 2309
Size: 399 306 22 10 46 28 81 26
Cuts at: 2309 2823 2901 2946 2955 3083 3354 3467 3539
Size: 514 78 45 9 128 271 113 72
Cuts at: 3539 3598 3928 3935 3960 4102 4266 4482 4565
Size: 59 330 7 25 142 164 216 83
Cuts at: 4565 4656 4913 4974 4993 5307 5490 5574 5633
Size: 91 257 61 19 314 183 84 59
Cuts at: 5633 6375 6460 7531 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 399 330 314 306
271 257 216 183 164 142 128 113
91 85 84 83 81 78 72 61
59 59 46 45 28 26 25 22
19 10 9 7
BspEI T'CCGG_A
Cuts at: 3630 7977 3630
Size: 4347 3967
BspHI T'CATG_A
Cuts at: 3285 6203 7933 3285
Size: 2918 1730 3666
Fragments arranged by size:
3666 2918 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2575 2598 4317 5669 844
Size: 1731 23 1719 1352 3489
Fragments arranged by size:
3489 1731 1719 1352 23
BsrBI CCG'CTC
Cuts at: 1394 1823 2427 4036 4296 5086 5326 6201 1394
Size: 429 604 1609 260 790 240 875 3507
Fragments arranged by size:
3507 1609 875 790 604 429 260 240
BsrDI GCAATG_nn'
Cuts at: 65 1248 5055 6809 6983 65
Size: 1183 3807 1754 174 1396
Fragments arranged by size:
3807 1754 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2337 2766 3467 3587 4209 4419 4465
Size: 110 527 429 701 120 622 210 46
Cuts at: 4465 5429 6958 7145 1700
Size: 964 1529 187 2869
Fragments arranged by size:
2869 1529 964 701 622 527 429 210
187 120 110 46
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8314
BssSI C'ACGA_G
Cuts at: 2211 2441 3094 3692 3996 4327 6374 7386 2211
Size: 230 653 598 304 331 2047 1012 3139
Fragments arranged by size:
3139 2047 1012 653 598 331 304 230
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3855 4243 5311 5383 794
Size: 3061 388 1068 72 3725
Fragments arranged by size:
3725 3061 1068 388 72
BstBI TT'CG_AA
Cuts at: 7118 7118
Size: 8314
BstEII G'GTnAC_C
Cuts at: 1184 3712 1184
Size: 2528 5786
BstXI CCAn_nnnn'nTGG
Cuts at: 2036 3441 4342 4738 5134 2036
Size: 1405 901 396 396 5216
Fragments arranged by size:
5216 1405 901 396 396
BstYI r'GATC_y
Cuts at: 1972 2565 3516 3736 3834 4690 4860 5644 6011
Size: 593 951 220 98 856 170 784 367
Cuts at: 6011 6396 6413 7865 7951 7963 8309 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 951 856 784 593 385 367
346 220 170 98 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3938 5279 3938
Size: 1341 6973
BtgI C'CryG_G
Cuts at: 513 1065 2004 2321 3273 4002 4482 5358 513
Size: 552 939 317 952 729 480 876 3469
Fragments arranged by size:
3469 952 939 876 729 552 480 317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5364 487
Size: 683 56 4138 3437
Fragments arranged by size:
4138 3437 683 56
BtsI GCAGTG_nn'
Cuts at: 3015 3207 4781 5574 5830 6624 6644 3015
Size: 192 1574 793 256 794 20 4685
Fragments arranged by size:
4685 1574 794 793 256 192 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8279
DraI TTT'AAA
Cuts at: 3493 5618 3493
Size: 2125 6189
DraIII CAC_nnn'GTG
Cuts at: 5311 5311
Size: 8314
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3175 7321 809
Size: 507 1859 4146 1802
Fragments arranged by size:
4146 1859 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2682 2713 3216
Size: 54 1425 50 275 18 852 31 503
Cuts at: 3216 3501 3589 3809 4072 5215 5761 6650 8279
Size: 285 88 220 263 1143 546 889 1629
Cuts at: 8279 8
Size: 43
Fragments arranged by size:
1629 1425 1143 889 852 546 503 285
275 263 220 88 54 50 43 31
18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 3809 5761 1537
Size: 275 1777 220 1952 4090
Fragments arranged by size:
4090 1952 1777 275 220
EarI CTCTTCn'nnn_
Cuts at: 1353 2012 2915 3632 3884 4644 6244 1353
Size: 659 903 717 252 760 1600 3423
Fragments arranged by size:
3423 1600 903 760 717 659 252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3511 3901 4285 6901 7275 7421
Size: 1064 138 1922 390 384 2616 374 146
Cuts at: 7421 387
Size: 1280
Fragments arranged by size:
2616 1922 1280 1064 390 384 374 146
138
EcoICRI GAG'CTC
Cuts at: 2172 2821 2172
Size: 649 7665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2969 2984 3046 3062 3152 3317 4037
Size: 393 1337 15 62 16 90 165 720
Cuts at: 4037 4159 4520 4694 4760 4769 4793 6377 6974
Size: 122 361 174 66 9 24 1584 597
Cuts at: 6974 7761 8029 1239
Size: 787 268 1524
Fragments arranged by size:
1584 1524 1337 787 720 597 393 361
268 174 165 122 90 66 62 24
16 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2715 4433 4685 2715
Size: 1718 252 6344
Fragments arranged by size:
6344 1718 252
EcoO109I rG'GnC_Cy
Cuts at: 2092 2125 2198 2280 2794 2887 3037 3652 3924
Size: 33 73 82 514 93 150 615 272
Cuts at: 3924 3925 4231 4459 4543 4678 4910 2092
Size: 1 306 228 84 135 232 5496
Fragments arranged by size:
5496 615 514 306 272 232 228 150
135 93 84 82 73 33 1
EcoRI G'AATT_C
Cuts at: 5623 6001 5623
Size: 378 7936
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3505 3856 3505
Size: 351 7963
FseI GG_CCGG'CC
Cuts at: 2341 2341
Size: 8314
FspI TGC'GCA
Cuts at: 5498 6820 5498
Size: 1322 6992
HaeII r_GCGC'y
Cuts at: 1678 2153 2262 2707 4201 4221 4442 4472 4772
Size: 475 109 445 1494 20 221 30 300
Cuts at: 4772 7461 1678
Size: 2689 2531
Fragments arranged by size:
2689 2531 1494 475 445 300 221 109
30 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2594 2626 3665 3697 4586
Size: 32 135 32 1022 32 1039 32 889
Cuts at: 4586 4618 6955 6987 7029 7061 1373
Size: 32 2337 32 42 32 2626
Fragments arranged by size:
2626 2337 1039 1022 889 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3400 5658 5904 669
Size: 988 281 1462 2258 246 3079
Fragments arranged by size:
3079 2258 1462 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8314
HpaI GTT'AAC
Cuts at: 5904 5904
Size: 8314
KasI G'GCGC_C
Cuts at: 4468 4468
Size: 8314
KpnI G_GTAC'C
Cuts at: 4210 5639 4210
Size: 1429 6885
MfeI C'AATT_G
Cuts at: 5913 5913
Size: 8314
MluI A'CGCG_T
Cuts at: 8190 8190
Size: 8314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3039 7428 7612 1305
Size: 1734 4389 184 2007
Fragments arranged by size:
4389 2007 1734 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3503 10
Size: 54 1425 343 1671 4821
Fragments arranged by size:
4821 1671 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2690 3254 3542 4343 4401 5036 5126
Size: 648 1524 564 288 801 58 635 90
Cuts at: 5126 5444 6272 6631 6790 518
Size: 318 828 359 159 2042
Fragments arranged by size:
2042 1524 828 801 648 635 564 359
318 288 159 90 58
MspA1I CmG'CkG
Cuts at: 1606 2371 2449 2553 2746 3616 3767 4048 4498
Size: 765 78 104 193 870 151 281 450
Cuts at: 4498 4725 4798 5730 6407 7555 7800 1606
Size: 227 73 932 677 1148 245 2120
Fragments arranged by size:
2120 1148 932 870 765 677 450 281
245 227 193 151 104 78 73
NaeI GCC'GGC
Cuts at: 2339 4467 5431 2339
Size: 2128 964 5222
Fragments arranged by size:
5222 2128 964
NarI GG'CG_CC
Cuts at: 4469 4469
Size: 8314
NcoI C'CATG_G
Cuts at: 513 1065 2004 5358 513
Size: 552 939 3354 3469
Fragments arranged by size:
3469 3354 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8314
NgoMIV G'CCGG_C
Cuts at: 2337 4465 5429 2337
Size: 2128 964 5222
Fragments arranged by size:
5222 2128 964
NheI G'CTAG_C
Cuts at: 1052 3238 1052
Size: 2186 6128
NotI GC'GGCC_GC
Cuts at: 5761 5761
Size: 8314
NspI r_CATG'y
Cuts at: 1132 1229 3022 5338 5672 7217 1132
Size: 97 1793 2316 334 1545 2229
Fragments arranged by size:
2316 2229 1793 1545 334 97
PasI CC'CwG_GG
Cuts at: 1348 2980 3928 4656 5574 1348
Size: 1632 948 728 918 4088
Fragments arranged by size:
4088 1632 948 918 728
PciI A'CATG_T
Cuts at: 5334 7213 5334
Size: 1879 6435
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5051 1108
Size: 3943 4371
PfoI T'CCnGG_A
Cuts at: 3129 4590 3129
Size: 1461 6853
PmeI GTTT'AAAC
Cuts at: 5618 5618
Size: 8314
PmlI CAC'GTG
Cuts at: 4261 4938 4261
Size: 677 7637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2501 2533 6383 6415 7896 7928 2501
Size: 32 3850 32 1481 32 2887
Fragments arranged by size:
3850 2887 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2794 3652 4459 4543 2794
Size: 858 807 84 6565
Fragments arranged by size:
6565 858 807 84
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8314
PsiI TTA'TAA
Cuts at: 3831 5884 3831
Size: 2053 6261
PspOMI G'GGCC_C
Cuts at: 2092 2124 2198 2279 3594 3924 4098 4909 2092
Size: 32 74 81 1315 330 174 811 5497
Fragments arranged by size:
5497 1315 811 330 174 81 74 32
PspXI vC'TCGA_Gb
Cuts at: 5321 5321
Size: 8314
PstI C_TGCA'G
Cuts at: 830 2586 2609 2886 3334 4047 4239 4380 5118
Size: 1756 23 277 448 713 192 141 738
Cuts at: 5118 5379 5666 830
Size: 261 287 3478
Fragments arranged by size:
3478 1756 738 713 448 287 277 261
192 141 23
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8314
PvuII CAG'CTG
Cuts at: 2371 2746 3616 3767 4048 4498 4798 2371
Size: 375 870 151 281 450 300 5887
Fragments arranged by size:
5887 870 450 375 300 281 151
SacI G_AGCT'C
Cuts at: 2174 2823 2174
Size: 649 7665
SalI G'TCGA_C
Cuts at: 1936 5656 1936
Size: 3720 4594
SanDI GG'GwC_CC
Cuts at: 2794 4543 2794
Size: 1749 6565
SapI GCTCTTCn'nnn_
Cuts at: 2012 4644 2012
Size: 2632 5682
SbfI CC_TGCA'GG
Cuts at: 5666 5666
Size: 8314
ScaI AGT'ACT
Cuts at: 2405 6562 8274 2405
Size: 4157 1712 2445
Fragments arranged by size:
4157 2445 1712
SfcI C'TryA_G
Cuts at: 826 1045 2330 2407 2582 2605 2882 3330 4043
Size: 219 1285 77 175 23 277 448 713
Cuts at: 4043 4235 4376 4872 5114 5375 5662 6797 7478
Size: 192 141 496 242 261 287 1135 681
Cuts at: 7478 7669 826
Size: 191 1471
Fragments arranged by size:
1471 1285 1135 713 681 496 448 287
277 261 242 219 192 191 175 141
77 23
SfoI GGC'GCC
Cuts at: 4470 4470
Size: 8314
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8314
SgrAI Cr'CCGG_yG
Cuts at: 1700 4465 1700
Size: 2765 5549
SmaI CCC'GGG
Cuts at: 1872 2387 2600 3650 3968 5240 5641 1872
Size: 515 213 1050 318 1272 401 4545
Fragments arranged by size:
4545 1272 1050 515 401 318 213
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2936 4952 5321 6418 7319 7581
Size: 197 926 993 2016 369 1097 901 262
Cuts at: 7581 820
Size: 1553
Fragments arranged by size:
2016 1553 1097 993 926 901 369 262
197
SnaBI TAC'GTA
Cuts at: 493 6105 493
Size: 5612 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8314
SphI G_CATG'C
Cuts at: 1132 5672 1132
Size: 4540 3774
SspI AAT'ATT
Cuts at: 5 52 6238 5
Size: 47 6186 2081
Fragments arranged by size:
6186 2081 47
StuI AGG'CCT
Cuts at: 1517 2534 4085 1517
Size: 1017 1551 5746
Fragments arranged by size:
5746 1551 1017
StyI C'CwwG_G
Cuts at: 513 1065 2004 2071 3459 4152 4641 5358 5753
Size: 552 939 67 1388 693 489 717 395
Cuts at: 5753 513
Size: 3074
Fragments arranged by size:
3074 1388 939 717 693 552 489 395
67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4525 4624 4808 4855 4862 4866 5516
Size: 1255 2945 99 184 47 7 4 650
Cuts at: 5516 5543 6341 6358 6511 6696 7141 325
Size: 27 798 17 153 185 445 1498
Fragments arranged by size:
2945 1498 1255 798 650 445 185 184
153 99 47 27 17 7 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2403 6560 8272
Size: 274 80 33 51 1443 426 4157 1712
Cuts at: 8272 96
Size: 138
Fragments arranged by size:
4157 1712 1443 426 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3091 3123 3436 3468 4700 4732 4953 4985 8274
Size: 32 313 32 1232 32 221 32 3289
Cuts at: 8274 8306 3091
Size: 32 3099
Fragments arranged by size:
3289 3099 1232 313 221 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8314
XbaI T'CTAG_A
Cuts at: 5650 5650
Size: 8314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3022 4480 3022
Size: 1458 6856
XhoI C'TCGA_G
Cuts at: 1943 5321 1943
Size: 3378 4936
XmaI C'CCGG_G
Cuts at: 1870 2385 2598 3648 3966 5238 5639 1870
Size: 515 213 1050 318 1272 401 4545
Fragments arranged by size:
4545 1272 1050 515 401 318 213
XmnI GAAnn'nnTTC
Cuts at: 4277 4718 6443 4277
Size: 441 1725 6148
Fragments arranged by size:
6148 1725 441
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5437 6122 276
Size: 53 83 186 936 3903 685 2468
Fragments arranged by size:
3903 2468 936 685 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
EcoRV FspAI NruI NsiI PacI PsrI RsrII SacII
SexAI SfiI SrfI SwaI