(Circular) (Six-base) MAPSORT of: pFN21AE5807.seq Check: 779 from: 1 to: 8923 pFN21AE5807 8923 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6733 278 Size: 53 83 186 936 5197 2468 Fragments arranged by size: 5197 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6266 1937 Size: 4329 4594 Acc65I G'GTAC_C Cuts at: 3346 6244 3346 Size: 2898 6025 AclI AA'CG_TT Cuts at: 1563 3380 3538 5604 7050 7423 1563 Size: 1817 158 2066 1446 373 3063 Fragments arranged by size: 3063 2066 1817 1446 373 158 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2112 2490 3053 3212 3266 3528 4650 4982 Size: 480 378 563 159 54 262 1122 332 Cuts at: 4982 5571 6986 8370 8638 1632 Size: 589 1415 1384 268 1917 Fragments arranged by size: 1917 1415 1384 1122 589 563 480 378 332 268 262 159 54 AflII C'TTAA_G Cuts at: 820 1017 2614 820 Size: 197 1597 7129 Fragments arranged by size: 7129 1597 197 AflIII A'CryG_T Cuts at: 1203 2074 7822 8799 1203 Size: 871 5748 977 1327 Fragments arranged by size: 5748 1327 977 871 AgeI A'CCGG_T Cuts at: 7754 7754 Size: 8923 AhdI GACnn_n'nnGTC Cuts at: 7652 7652 Size: 8923 AleI CACnn'nnGTG Cuts at: 1166 2429 5852 1166 Size: 1263 3423 4237 Fragments arranged by size: 4237 3423 1263 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2789 3276 1184 Size: 1605 487 6831 Fragments arranged by size: 6831 1605 487 AlwNI CAG_nnn'CTG Cuts at: 2317 3004 3595 6666 8238 2317 Size: 687 591 3071 1572 3002 Fragments arranged by size: 3071 3002 1572 687 591 ApaI G_GGCC'C Cuts at: 2063 2063 Size: 8923 ApaLI G'TGCA_C Cuts at: 5799 6980 8136 5799 Size: 1181 1156 6586 Fragments arranged by size: 6586 1181 1156 ApoI r'AATT_y Cuts at: 1493 2375 2496 2528 2624 2645 4490 5125 5215 Size: 882 121 32 96 21 1845 635 90 Cuts at: 5215 5360 5465 5482 6232 6458 6610 8719 1493 Size: 145 105 17 750 226 152 2109 1697 Fragments arranged by size: 2109 1845 1697 882 750 635 226 152 145 121 105 96 90 32 21 17 AseI AT'TA_AT Cuts at: 160 1032 4157 7477 160 Size: 872 3125 3320 1606 Fragments arranged by size: 3320 3125 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8923 AvaI C'yCGr_G Cuts at: 1870 1943 2119 6248 1870 Size: 73 176 4129 4545 Fragments arranged by size: 4545 4129 176 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5624 5657 5624 Size: 33 8890 BamHI G'GATC_C Cuts at: 6253 6620 6253 Size: 367 8556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3346 6244 7699 618 Size: 325 217 627 1559 2898 1455 1842 Fragments arranged by size: 2898 1842 1559 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2063 3566 6242 1391 Size: 672 1503 2676 4072 Fragments arranged by size: 4072 2676 1503 672 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2868 4740 5357 5813 928 Size: 971 969 1872 617 456 4038 Fragments arranged by size: 4038 1872 971 969 617 456 BbvCI CC'TCA_GC Cuts at: 2243 4583 2243 Size: 2340 6583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7114 7148 1903 Size: 34 5177 34 3678 Fragments arranged by size: 5177 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5089 5232 6815 8025 5089 Size: 143 1583 1210 5987 Fragments arranged by size: 5987 1583 1210 143 BclI T'GATC_A Cuts at: 1548 4377 1548 Size: 2829 6094 BfrBI ATG'CAT Cuts at: 4352 5401 4352 Size: 1049 7874 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 7534 136 Size: 107 122 71 631 605 337 5525 1525 Fragments arranged by size: 5525 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2419 4004 4448 8918 2419 Size: 1585 444 4470 2424 Fragments arranged by size: 4470 2424 1585 444 BlpI GC'TnA_GC Cuts at: 2330 6340 2330 Size: 4010 4913 Bme1580I G_kGCm'C Cuts at: 1790 2063 5803 6984 8140 1790 Size: 273 3740 1181 1156 2573 Fragments arranged by size: 3740 2573 1181 1156 273 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6654 7612 442 Size: 416 53 83 399 24 5237 958 1753 Fragments arranged by size: 5237 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8923 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2296 4053 2296 Size: 1757 7166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2339 2387 2753 2807 4023 4325 7583 1239 Size: 1100 48 366 54 1216 302 3258 2579 Fragments arranged by size: 3258 2579 1216 1100 366 302 54 48 Bpu10I CC'TnA_GC Cuts at: 2243 4583 2243 Size: 2340 6583 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3300 5663 7048 7913 8211 3300 Size: 2363 1385 865 298 4012 Fragments arranged by size: 4012 2363 1385 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4208 7586 882 Size: 617 2709 3378 2219 Fragments arranged by size: 3378 2709 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2075 6714 493 Size: 711 871 4639 2702 Fragments arranged by size: 4639 2702 871 711 BsaBI GATnn'nnATC Cuts at: 8917 8917 Size: 8923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5943 6730 7112 7747 Size: 53 83 186 936 4410 787 382 635 Cuts at: 7747 275 Size: 1451 Fragments arranged by size: 4410 1451 936 787 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7356 7754 8028 8175 8586 7356 Size: 398 274 147 411 7693 Fragments arranged by size: 7693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2079 2109 1178 Size: 30 165 30 676 30 7992 Fragments arranged by size: 7992 676 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2303 2360 2750 4045 4342 5051 5672 Size: 1045 72 57 390 1295 297 709 621 Cuts at: 5672 1186 Size: 4437 Fragments arranged by size: 4437 1295 1045 709 621 390 297 72 57 BseYI C'CCAG_C Cuts at: 2044 2056 3042 4596 5138 8126 8622 2044 Size: 12 986 1554 542 2988 496 2345 Fragments arranged by size: 2988 2345 1554 986 542 496 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2749 1163 Size: 573 1013 7337 Fragments arranged by size: 7337 1013 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6373 7134 8162 1540 Size: 275 186 4372 761 1028 2301 Fragments arranged by size: 4372 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3301 3566 5803 6984 7069 8140 3301 Size: 265 2237 1181 85 1071 4084 Fragments arranged by size: 4084 2237 1181 1071 265 85 BsmI GAATG_Cn' Cuts at: 4825 5008 5317 6434 6527 4825 Size: 183 309 1117 93 7221 Fragments arranged by size: 7221 1117 309 183 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2405 2405 Size: 8923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 3301 3566 5803 6242 6984 7069 Size: 399 273 1238 265 2237 439 742 85 Cuts at: 7069 8140 1391 Size: 1071 2174 Fragments arranged by size: 2237 2174 1238 1071 742 439 399 273 265 85 BspEI T'CCGG_A Cuts at: 8586 8586 Size: 8923 BspHI T'CATG_A Cuts at: 3495 4810 6812 8542 3495 Size: 1315 2002 1730 3876 Fragments arranged by size: 3876 2002 1730 1315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2781 6278 844 Size: 1937 3497 3489 Fragments arranged by size: 3497 3489 1937 BsrBI CCG'CTC Cuts at: 1394 1823 6810 1394 Size: 429 4987 3507 Fragments arranged by size: 4987 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2109 4573 5730 5771 5810 7418 7592 Size: 1183 861 2464 1157 41 39 1608 174 Cuts at: 7592 65 Size: 1396 Fragments arranged by size: 2464 1608 1396 1183 1157 861 174 41 39 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 2199 7567 7754 1700 Size: 110 319 70 5368 187 2869 Fragments arranged by size: 5368 2869 319 187 110 70 BsrGI T'GTAC_A Cuts at: 96 2689 6096 96 Size: 2593 3407 2923 Fragments arranged by size: 3407 2923 2593 BssHII G'CGCG_C Cuts at: 1925 2247 1925 Size: 322 8601 BssSI C'ACGA_G Cuts at: 6983 7995 6983 Size: 1012 7911 BstAPI GCAn_nnn'nTGC Cuts at: 794 3004 794 Size: 2210 6713 BstBI TT'CG_AA Cuts at: 7727 7727 Size: 8923 BstEII G'GTnAC_C Cuts at: 1184 2299 1184 Size: 1115 7808 BstYI r'GATC_y Cuts at: 1972 2419 3799 4004 4448 5674 6253 6620 7005 Size: 447 1380 205 444 1226 579 367 385 Cuts at: 7005 7022 8474 8560 8572 8918 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1380 1226 579 447 444 385 367 346 205 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4325 4325 Size: 8923 BtgI C'CryG_G Cuts at: 513 1065 2004 2253 5234 513 Size: 552 939 249 2981 4202 Fragments arranged by size: 4202 2981 939 552 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2309 3338 487 Size: 683 56 1083 1029 6072 Fragments arranged by size: 6072 1083 1029 683 56 BtsI GCAGTG_nn' Cuts at: 3769 4627 6439 7233 7253 3769 Size: 858 1812 794 20 5439 Fragments arranged by size: 5439 1812 858 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8888 DraI TTT'AAA Cuts at: 2650 4495 4776 6227 2650 Size: 1845 281 1451 5346 Fragments arranged by size: 5346 1845 1451 281 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7930 809 Size: 507 6614 1802 Fragments arranged by size: 6614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4431 5865 6370 Size: 54 1425 50 275 18 2601 1434 505 Cuts at: 6370 7259 8888 8 Size: 889 1629 43 Fragments arranged by size: 2601 1629 1434 1425 889 505 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6370 1537 Size: 275 4558 4090 Fragments arranged by size: 4558 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2319 2697 3309 3576 3979 4365 5832 6853 Size: 966 378 612 267 403 386 1467 1021 Cuts at: 6853 1353 Size: 3423 Fragments arranged by size: 3423 1467 1021 966 612 403 386 378 267 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7510 7884 8030 387 Size: 1064 138 5921 374 146 1280 Fragments arranged by size: 5921 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3564 3564 Size: 8923 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 2339 2387 2490 2753 2807 3053 Size: 393 480 227 48 103 263 54 246 Cuts at: 3053 3212 3266 3528 4023 4325 4650 4982 5571 Size: 159 54 262 495 302 325 332 589 Cuts at: 5571 6986 7583 8370 8638 1239 Size: 1415 597 787 268 1524 Fragments arranged by size: 1524 1415 787 597 589 495 480 393 332 325 302 268 263 262 246 227 159 103 54 54 48 EcoNI CCTnn'n_nnAGG Cuts at: 2342 2360 4586 2342 Size: 18 2226 6679 Fragments arranged by size: 6679 2226 18 EcoO109I rG'GnC_Cy Cuts at: 2060 2067 2207 5788 2060 Size: 7 140 3581 5195 Fragments arranged by size: 5195 3581 140 7 EcoRI G'AATT_C Cuts at: 2624 6232 6610 2624 Size: 3608 378 4937 Fragments arranged by size: 4937 3608 378 EcoRV GAT'ATC Cuts at: 5412 5412 Size: 8923 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3296 6140 3296 Size: 2844 6079 FspI TGC'GCA Cuts at: 2108 7429 2108 Size: 5321 3602 HaeII r_GCGC'y Cuts at: 1678 8070 1678 Size: 6392 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3784 3816 4109 4141 5171 Size: 32 135 32 2212 32 293 32 1030 Cuts at: 5171 5203 5702 5734 7564 7596 7638 7670 1373 Size: 32 499 32 1830 32 42 32 2626 Fragments arranged by size: 2626 2212 1830 1030 499 293 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4897 6267 6513 669 Size: 988 281 2959 1370 246 3079 Fragments arranged by size: 3079 2959 1370 988 281 246 HindIII A'AGCT_T Cuts at: 748 3370 5681 748 Size: 2622 2311 3990 Fragments arranged by size: 3990 2622 2311 HpaI GTT'AAC Cuts at: 6513 6513 Size: 8923 KpnI G_GTAC'C Cuts at: 3350 6248 3350 Size: 2898 6025 MfeI C'AATT_G Cuts at: 5291 5577 6522 5291 Size: 286 945 7692 Fragments arranged by size: 7692 945 286 MluI A'CGCG_T Cuts at: 8799 8799 Size: 8923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4044 4074 4751 5586 8037 8221 1305 Size: 2739 30 677 835 2451 184 2007 Fragments arranged by size: 2739 2451 2007 835 677 184 30 MscI TGG'CCA Cuts at: 10 64 1489 1832 4433 5867 10 Size: 54 1425 343 2601 1434 3066 Fragments arranged by size: 3066 2601 1434 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2429 4442 5852 6206 6881 7240 7399 Size: 648 1263 2013 1410 354 675 359 159 Cuts at: 7399 518 Size: 2042 Fragments arranged by size: 2042 2013 1410 1263 675 648 359 354 159 MspA1I CmG'CkG Cuts at: 1606 2056 2314 2763 3128 6339 7016 8164 8409 Size: 450 258 449 365 3211 677 1148 245 Cuts at: 8409 1606 Size: 2120 Fragments arranged by size: 3211 2120 1148 677 450 449 365 258 245 NaeI GCC'GGC Cuts at: 2131 2201 2131 Size: 70 8853 NcoI C'CATG_G Cuts at: 513 1065 2004 2253 513 Size: 552 939 249 7183 Fragments arranged by size: 7183 939 552 249 NdeI CA'TA_TG Cuts at: 387 387 Size: 8923 NgoMIV G'CCGG_C Cuts at: 2129 2199 2129 Size: 70 8853 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8923 NotI GC'GGCC_GC Cuts at: 6370 6370 Size: 8923 NsiI A_TGCA'T Cuts at: 4354 5403 4354 Size: 1049 7874 NspI r_CATG'y Cuts at: 1132 1229 6281 7826 1132 Size: 97 5052 1545 2229 Fragments arranged by size: 5052 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8923 PciI A'CATG_T Cuts at: 7822 7822 Size: 8923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2078 5977 1108 Size: 970 3899 4054 Fragments arranged by size: 4054 3899 970 PmeI GTTT'AAAC Cuts at: 6227 6227 Size: 8923 PmlI CAC'GTG Cuts at: 2075 2075 Size: 8923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6992 7024 8505 8537 6992 Size: 32 1481 32 7378 Fragments arranged by size: 7378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2067 5788 2067 Size: 3721 5202 PshAI GACnn'nnGTC Cuts at: 1613 4313 1613 Size: 2700 6223 PsiI TTA'TAA Cuts at: 2723 6493 2723 Size: 3770 5153 PspOMI G'GGCC_C Cuts at: 2059 2059 Size: 8923 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3909 3941 3909 Size: 32 8891 PstI C_TGCA'G Cuts at: 830 3000 3009 6275 830 Size: 2170 9 3266 3478 Fragments arranged by size: 3478 3266 2170 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8923 PvuII CAG'CTG Cuts at: 2056 2314 3128 2056 Size: 258 814 7851 Fragments arranged by size: 7851 814 258 SacI G_AGCT'C Cuts at: 3566 3566 Size: 8923 SalI G'TCGA_C Cuts at: 1936 6265 1936 Size: 4329 4594 SapI GCTCTTCn'nnn_ Cuts at: 2319 2697 3576 4365 2319 Size: 378 879 789 6877 Fragments arranged by size: 6877 879 789 378 SbfI CC_TGCA'GG Cuts at: 6275 6275 Size: 8923 ScaI AGT'ACT Cuts at: 2550 3099 4036 6156 7171 8883 2550 Size: 549 937 2120 1015 1712 2590 Fragments arranged by size: 2590 2120 1712 1015 937 549 SfcI C'TryA_G Cuts at: 826 1045 2996 3005 3587 3701 3721 6271 7406 Size: 219 1951 9 582 114 20 2550 1135 Cuts at: 7406 8087 8278 826 Size: 681 191 1471 Fragments arranged by size: 2550 1951 1471 1135 681 582 219 191 114 20 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8923 SmaI CCC'GGG Cuts at: 1872 6250 1872 Size: 4378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2614 3315 5678 7027 7928 8190 Size: 197 926 671 701 2363 1349 901 262 Cuts at: 8190 820 Size: 1553 Fragments arranged by size: 2363 1553 1349 926 901 701 671 262 197 SnaBI TAC'GTA Cuts at: 493 6714 493 Size: 6221 2702 SpeI A'CTAG_T Cuts at: 152 2716 6184 152 Size: 2564 3468 2891 Fragments arranged by size: 3468 2891 2564 SphI G_CATG'C Cuts at: 1132 6281 1132 Size: 5149 3774 SspI AAT'ATT Cuts at: 5 52 4945 6024 6847 5 Size: 47 4893 1079 823 2081 Fragments arranged by size: 4893 2081 1079 823 47 StuI AGG'CCT Cuts at: 1517 2117 1517 Size: 600 8323 StyI C'CwwG_G Cuts at: 513 1065 2004 2253 4482 5976 6362 513 Size: 552 939 249 2229 1494 386 3074 Fragments arranged by size: 3074 2229 1494 939 552 386 249 SwaI ATTT'AAAT Cuts at: 4776 4776 Size: 8923 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2164 2168 6950 6967 7120 7305 7750 Size: 1255 584 4 4782 17 153 185 445 Cuts at: 7750 325 Size: 1498 Fragments arranged by size: 4782 1498 1255 584 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2095 2548 2689 Size: 274 80 33 51 1443 118 453 141 Cuts at: 2689 3029 3097 3925 4034 4762 4908 4993 6096 Size: 340 68 828 109 728 146 85 1103 Cuts at: 6096 6154 7169 8881 96 Size: 58 1015 1712 138 Fragments arranged by size: 1712 1443 1103 1015 828 728 453 340 274 146 141 138 118 109 85 80 68 58 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8883 8915 8883 Size: 32 8891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8923 XbaI T'CTAG_A Cuts at: 2491 6259 2491 Size: 3768 5155 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8923 XmaI C'CCGG_G Cuts at: 1870 6248 1870 Size: 4378 4545 XmnI GAAnn'nnTTC Cuts at: 2624 3117 3279 7052 2624 Size: 493 162 3773 4495 Fragments arranged by size: 4495 3773 493 162 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6731 276 Size: 53 83 186 936 5197 2468 Fragments arranged by size: 5197 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BsiWI BstXI BstZ17I DraIII FseI FspAI KasI NarI NruI PacI PfoI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI