Restriction map for
pFN21AE5792
(Circular) (Six-base) MAPSORT of: pFN21AE5792.seq Check: 8360 from: 1 to: 6673
pFN21AE5792 6673 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4483 278
Size: 53 83 186 936 2947 2468
Fragments arranged by size:
2947 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3237 3602 4016 1937
Size: 1300 365 414 4594
Fragments arranged by size:
4594 1300 414 365
Acc65I G'GTAC_C
Cuts at: 3994 3994
Size: 6673
AclI AA'CG_TT
Cuts at: 1563 2340 4800 5173 1563
Size: 777 2460 373 3063
Fragments arranged by size:
3063 2460 777 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2421 2445 3080 3834 4736 6120 6388 1632
Size: 789 24 635 754 902 1384 268 1917
Fragments arranged by size:
1917 1384 902 789 754 635 268 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6476
AflIII A'CryG_T
Cuts at: 1203 3536 5572 6549 1203
Size: 2333 2036 977 1327
Fragments arranged by size:
2333 2036 1327 977
AgeI A'CCGG_T
Cuts at: 5504 5504
Size: 6673
AhdI GACnn_n'nnGTC
Cuts at: 5402 5402
Size: 6673
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6673
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3048 1184
Size: 1864 4809
AlwNI CAG_nnn'CTG
Cuts at: 2239 4416 5988 2239
Size: 2177 1572 2924
Fragments arranged by size:
2924 2177 1572
ApaLI G'TGCA_C
Cuts at: 4730 5886 4730
Size: 1156 5517
ApoI r'AATT_y
Cuts at: 1493 2777 2900 3156 3818 3982 4208 4360 6469
Size: 1284 123 256 662 164 226 152 2109
Cuts at: 6469 1493
Size: 1697
Fragments arranged by size:
2109 1697 1284 662 256 226 164 152
123
AseI AT'TA_AT
Cuts at: 160 1032 5227 160
Size: 872 4195 1606
Fragments arranged by size:
4195 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6673
AvaI C'yCGr_G
Cuts at: 1870 1943 3682 3998 1870
Size: 73 1739 316 4545
Fragments arranged by size:
4545 1739 316 73
BamHI G'GATC_C
Cuts at: 4003 4370 4003
Size: 367 6306
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3994 5449 618
Size: 325 217 627 2207 1455 1842
Fragments arranged by size:
2207 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3658 3992 1391
Size: 2267 334 4072
Fragments arranged by size:
4072 2267 334
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3200 3321 3570 3852 928
Size: 971 1301 121 249 282 3749
Fragments arranged by size:
3749 1301 971 282 249 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2447 2481 4864 4898 1903
Size: 34 510 34 2383 34 3678
Fragments arranged by size:
3678 2383 510 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4565 5775 4565
Size: 1210 5463
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6673
BfrBI ATG'CAT
Cuts at: 3507 3507
Size: 6673
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5284 136
Size: 107 122 71 631 605 3612 1525
Fragments arranged by size:
3612 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3363 3801 6668 3363
Size: 438 2867 3368
Fragments arranged by size:
3368 2867 438
BlpI GC'TnA_GC
Cuts at: 4090 4090
Size: 6673
Bme1580I G_kGCm'C
Cuts at: 1790 4734 5890 1790
Size: 2944 1156 2573
Fragments arranged by size:
2944 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6673
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2152 4404 5362
Size: 416 53 83 399 24 735 2252 958
Cuts at: 5362 442
Size: 1753
Fragments arranged by size:
2252 1753 958 735 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3429 5333 1239
Size: 2190 1904 2579
Fragments arranged by size:
2579 2190 1904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2709 4798 5663 5961 2709
Size: 2089 865 298 3421
Fragments arranged by size:
3421 2089 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5336 882
Size: 617 3837 2219
Fragments arranged by size:
3837 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4464 493
Size: 711 3260 2702
Fragments arranged by size:
3260 2702 711
BsaBI GATnn'nnATC
Cuts at: 2170 3414 6667 2170
Size: 1244 3253 2176
Fragments arranged by size:
3253 2176 1244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4480 4862 5497 275
Size: 53 83 186 936 2947 382 635 1451
Fragments arranged by size:
2947 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5106 5504 5778 5925 6336 5106
Size: 398 274 147 411 5443
Fragments arranged by size:
5443 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6448
Fragments arranged by size:
6448 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6673
BseYI C'CCAG_C
Cuts at: 5876 6372 5876
Size: 496 6177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3008 3204 3371 1163
Size: 573 1272 196 167 4465
Fragments arranged by size:
4465 1272 573 196 167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4123 4884 5912 1540
Size: 275 186 2122 761 1028 2301
Fragments arranged by size:
2301 2122 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2359 4734 4819 5890 2359
Size: 2375 85 1071 3142
Fragments arranged by size:
3142 2375 1071 85
BsmI GAATG_Cn'
Cuts at: 4184 4277 4184
Size: 93 6580
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2359 3658 3992 4734 4819 5890 1391
Size: 399 569 1299 334 742 85 1071 2174
Fragments arranged by size:
2174 1299 1071 742 569 399 334 85
BspEI T'CCGG_A
Cuts at: 6336 6336
Size: 6673
BspHI T'CATG_A
Cuts at: 4562 6292 4562
Size: 1730 4943
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4028 844
Size: 3184 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4560 1394
Size: 429 2737 3507
Fragments arranged by size:
3507 2737 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3042 5168 5342 65
Size: 1183 1794 2126 174 1396
Fragments arranged by size:
2126 1794 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5317 5504 1700
Size: 110 3507 187 2869
Fragments arranged by size:
3507 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6673
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6673
BssSI C'ACGA_G
Cuts at: 4733 5745 4733
Size: 1012 5661
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6673
BstBI TT'CG_AA
Cuts at: 5477 5477
Size: 6673
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6673
BstYI r'GATC_y
Cuts at: 1972 3363 3801 4003 4370 4755 4772 6224 6310
Size: 1391 438 202 367 385 17 1452 86
Cuts at: 6310 6322 6668 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1391 438 385 367 346 202
86 17 12
BstZ17I GTA'TAC
Cuts at: 3603 3603
Size: 6673
BtgI C'CryG_G
Cuts at: 513 1065 2004 2874 513
Size: 552 939 870 4312
Fragments arranged by size:
4312 939 870 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5934
Fragments arranged by size:
5934 683 56
BtsI GCAGTG_nn'
Cuts at: 2966 4189 4983 5003 2966
Size: 1223 794 20 4636
Fragments arranged by size:
4636 1223 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6673
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2011 2046 529
Size: 35 1447 35 5156
Fragments arranged by size:
5156 1447 35 35
DraI TTT'AAA
Cuts at: 2782 3977 2782
Size: 1195 5478
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5680 809
Size: 507 4364 1802
Fragments arranged by size:
4364 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3145 3909 4120
Size: 54 1425 50 275 18 1315 764 211
Cuts at: 4120 5009 6638 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1315 889 764 275 211 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4120 1537
Size: 275 2308 4090
Fragments arranged by size:
4090 2308 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2274 2340 4603 1353
Size: 921 66 2263 3423
Fragments arranged by size:
3423 2263 921 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5260 5634 5780 387
Size: 1064 138 435 3236 374 146 1280
Fragments arranged by size:
3236 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2421 2445 3080 3429 3834 4736 5333
Size: 393 789 24 635 349 405 902 597
Cuts at: 5333 6120 6388 1239
Size: 787 268 1524
Fragments arranged by size:
1524 902 789 787 635 597 405 393
349 268 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3329 3329
Size: 6673
EcoO109I rG'GnC_Cy
Cuts at: 2236 2236
Size: 6673
EcoRI G'AATT_C
Cuts at: 3818 3982 4360 3818
Size: 164 378 6131
Fragments arranged by size:
6131 378 164
FspI TGC'GCA
Cuts at: 5179 5179
Size: 6673
HaeII r_GCGC'y
Cuts at: 1678 5820 1678
Size: 4142 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2341 2373 2690 2722 2835
Size: 32 135 32 769 32 317 32 113
Cuts at: 2835 2867 3230 3262 3427 3459 3557 3589 5314
Size: 32 363 32 165 32 98 32 1725
Cuts at: 5314 5346 5388 5420 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1725 769 363 317 165 135 113
98 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3238 4017 4263 669
Size: 988 281 1300 779 246 3079
Fragments arranged by size:
3079 1300 988 779 281 246
HindIII A'AGCT_T
Cuts at: 748 2304 2949 748
Size: 1556 645 4472
Fragments arranged by size:
4472 1556 645
HpaI GTT'AAC
Cuts at: 4263 4263
Size: 6673
KpnI G_GTAC'C
Cuts at: 3998 3998
Size: 6673
MfeI C'AATT_G
Cuts at: 3030 4272 3030
Size: 1242 5431
MluI A'CGCG_T
Cuts at: 6549 6549
Size: 6673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3833 5787 5971 1305
Size: 2528 1954 184 2007
Fragments arranged by size:
2528 2007 1954 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3147 3911 10
Size: 54 1425 343 1315 764 2772
Fragments arranged by size:
2772 1425 1315 764 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2937 4631 4990 5149 518
Size: 648 1771 1694 359 159 2042
Fragments arranged by size:
2042 1771 1694 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2078 4089 4766 5914 6159 1606
Size: 472 2011 677 1148 245 2120
Fragments arranged by size:
2120 2011 1148 677 472 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2874 513
Size: 552 939 870 4312
Fragments arranged by size:
4312 939 870 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6673
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6673
NotI GC'GGCC_GC
Cuts at: 4120 4120
Size: 6673
NsiI A_TGCA'T
Cuts at: 3509 3509
Size: 6673
NspI r_CATG'y
Cuts at: 1132 1229 2085 3342 3507 3540 3770 4031 5576
Size: 97 856 1257 165 33 230 261 1545
Cuts at: 5576 1132
Size: 2229
Fragments arranged by size:
2229 1545 1257 856 261 230 165 97
33
PasI CC'CwG_GG
Cuts at: 1348 3163 1348
Size: 1815 4858
PciI A'CATG_T
Cuts at: 3536 5572 3536
Size: 2036 4637
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6673
PfoI T'CCnGG_A
Cuts at: 3795 3886 3795
Size: 91 6582
PmeI GTTT'AAAC
Cuts at: 3977 3977
Size: 6673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4742 4774 6255 6287 4742
Size: 32 1481 32 5128
Fragments arranged by size:
5128 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2236 2236
Size: 6673
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6673
PsiI TTA'TAA
Cuts at: 2042 4243 2042
Size: 2201 4472
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2814 2846 2814
Size: 32 6641
PstI C_TGCA'G
Cuts at: 830 2364 4025 830
Size: 1534 1661 3478
Fragments arranged by size:
3478 1661 1534
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6673
PvuII CAG'CTG
Cuts at: 2078 2078
Size: 6673
SalI G'TCGA_C
Cuts at: 1936 3236 4015 1936
Size: 1300 779 4594
Fragments arranged by size:
4594 1300 779
SbfI CC_TGCA'GG
Cuts at: 4025 4025
Size: 6673
ScaI AGT'ACT
Cuts at: 2221 3378 4921 6633 2221
Size: 1157 1543 1712 2261
Fragments arranged by size:
2261 1712 1543 1157
SfcI C'TryA_G
Cuts at: 826 1045 2360 2613 3172 4021 5156 5837 6028
Size: 219 1315 253 559 849 1135 681 191
Cuts at: 6028 826
Size: 1471
Fragments arranged by size:
1471 1315 1135 849 681 559 253 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6673
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6673
SmaI CCC'GGG
Cuts at: 1872 4000 1872
Size: 2128 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2724 4777 5678 5940 820
Size: 197 926 781 2053 901 262 1553
Fragments arranged by size:
2053 1553 926 901 781 262 197
SnaBI TAC'GTA
Cuts at: 493 4464 493
Size: 3971 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6673
SphI G_CATG'C
Cuts at: 1132 3770 4031 1132
Size: 2638 261 3774
Fragments arranged by size:
3774 2638 261
SspI AAT'ATT
Cuts at: 5 52 2695 3302 4597 5
Size: 47 2643 607 1295 2081
Fragments arranged by size:
2643 2081 1295 607 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6673
StyI C'CwwG_G
Cuts at: 513 1065 2004 2874 3148 4112 513
Size: 552 939 870 274 964 3074
Fragments arranged by size:
3074 964 939 870 552 274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4700 4717 4870 5055 5500 325
Size: 1255 3120 17 153 185 445 1498
Fragments arranged by size:
3120 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2219 3019 3220
Size: 274 80 33 51 1443 242 800 201
Cuts at: 3220 3376 4919 6631 96
Size: 156 1543 1712 138
Fragments arranged by size:
1712 1543 1443 800 274 242 201 156
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6633 6665 6633
Size: 32 6641
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6673
XbaI T'CTAG_A
Cuts at: 4009 4009
Size: 6673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2326 3648 2326
Size: 1322 5351
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6673
XmaI C'CCGG_G
Cuts at: 1870 3998 1870
Size: 2128 4545
XmnI GAAnn'nnTTC
Cuts at: 2289 2829 3590 4802 2289
Size: 540 761 1212 4160
Fragments arranged by size:
4160 1212 761 540
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4481 276
Size: 53 83 186 936 2947 2468
Fragments arranged by size:
2947 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI