Restriction map for
pFN21AE5789
(Circular) (Six-base) MAPSORT of: pFN21AE5789.seq Check: 9398 from: 1 to: 6553
pFN21AE5789 6553 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3449 3449
Size: 6553
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4363 278
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2148 3217 3616 3631 3646 3896 1937
Size: 211 1069 399 15 15 250 4594
Fragments arranged by size:
4594 1069 399 250 211 15 15
Acc65I G'GTAC_C
Cuts at: 3874 3874
Size: 6553
AclI AA'CG_TT
Cuts at: 1563 4680 5053 1563
Size: 3117 373 3063
Fragments arranged by size:
3117 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4616 6000 6268 1632
Size: 2984 1384 268 1917
Fragments arranged by size:
2984 1917 1384 268
AfeI AGC'GCT
Cuts at: 2262 2262
Size: 6553
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6356
AflIII A'CryG_T
Cuts at: 1203 2484 2590 3293 3295 5452 6429 1203
Size: 1281 106 703 2 2157 977 1327
Fragments arranged by size:
2157 1327 1281 977 703 106 2
AgeI A'CCGG_T
Cuts at: 5384 5384
Size: 6553
AhdI GACnn_n'nnGTC
Cuts at: 5282 5282
Size: 6553
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6553
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2667 2699 2667
Size: 32 6521
AlwNI CAG_nnn'CTG
Cuts at: 3041 4296 5868 3041
Size: 1255 1572 3726
Fragments arranged by size:
3726 1572 1255
ApaI G_GGCC'C
Cuts at: 3392 3392
Size: 6553
ApaLI G'TGCA_C
Cuts at: 4610 5766 4610
Size: 1156 5397
ApoI r'AATT_y
Cuts at: 1493 2571 2787 3426 3862 4088 4240 6349 1493
Size: 1078 216 639 436 226 152 2109 1697
Fragments arranged by size:
2109 1697 1078 639 436 226 216 152
AseI AT'TA_AT
Cuts at: 160 1032 3447 5107 160
Size: 872 2415 1660 1606
Fragments arranged by size:
2415 1660 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
AvaI C'yCGr_G
Cuts at: 1870 1943 2982 3249 3878 1870
Size: 73 1039 267 629 4545
Fragments arranged by size:
4545 1039 629 267 73
BamHI G'GATC_C
Cuts at: 2353 2551 3883 4250 2353
Size: 198 1332 367 4656
Fragments arranged by size:
4656 1332 367 198
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3874 5329 618
Size: 325 217 627 2087 1455 1842
Fragments arranged by size:
2087 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2667 2941 3057 3189 3392 3872 1391
Size: 1276 274 116 132 203 480 4072
Fragments arranged by size:
4072 1276 480 274 203 132 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2743 928
Size: 971 844 4738
Fragments arranged by size:
4738 971 844
BbvCI CC'TCA_GC
Cuts at: 2715 2721 2715
Size: 6 6547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4744 4778 1903
Size: 34 2807 34 3678
Fragments arranged by size:
3678 2807 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 4445 5655 3818
Size: 627 1210 4716
Fragments arranged by size:
4716 1210 627
BclI T'GATC_A
Cuts at: 1548 3741 1548
Size: 2193 4360
BfrBI ATG'CAT
Cuts at: 3097 3097
Size: 6553
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5164 136
Size: 107 122 71 631 605 3492 1525
Fragments arranged by size:
3492 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6548 6548
Size: 6553
BlpI GC'TnA_GC
Cuts at: 3970 3970
Size: 6553
Bme1580I G_kGCm'C
Cuts at: 1790 3392 4614 5770 1790
Size: 1602 1222 1156 2573
Fragments arranged by size:
2573 1602 1222 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6553
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2888 3393 4284
Size: 416 53 83 399 24 1471 505 891
Cuts at: 4284 5242 442
Size: 958 1753
Fragments arranged by size:
1753 1471 958 891 505 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2691 2781 2871 3063 3537 3864 5213 1239
Size: 1452 90 90 192 474 327 1349 2579
Fragments arranged by size:
2579 1452 1349 474 327 192 90 90
Bpu10I CC'TnA_GC
Cuts at: 2715 2721 3792 2715
Size: 6 1071 5476
Fragments arranged by size:
5476 1071 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4678 5543 5841 4678
Size: 865 298 5390
Fragments arranged by size:
5390 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2019 2201 3314 5216 882
Size: 617 520 182 1113 1902 2219
Fragments arranged by size:
2219 1902 1113 617 520 182
BsaAI yAC'GTr
Cuts at: 493 1204 3017 3296 4344 493
Size: 711 1813 279 1048 2702
Fragments arranged by size:
2702 1813 1048 711 279
BsaBI GATnn'nnATC
Cuts at: 2543 6547 2543
Size: 4004 2549
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4360 4742 5377 275
Size: 53 83 186 936 2827 382 635 1451
Fragments arranged by size:
2827 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4986 5384 5658 5805 6216 4986
Size: 398 274 147 411 5323
Fragments arranged by size:
5323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2438 3837 3867 1178
Size: 30 165 30 1005 30 1399 30 3864
Fragments arranged by size:
3864 1399 1005 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2315 2703 2748 2868 2970 3322 3459
Size: 919 210 388 45 120 102 352 137
Cuts at: 3459 3594 3639 3654 1186
Size: 135 45 15 4085
Fragments arranged by size:
4085 919 388 352 210 137 135 120
102 45 45 15
BseYI C'CCAG_C
Cuts at: 5756 6252 5756
Size: 496 6057
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2939 1163
Size: 573 1203 4777
Fragments arranged by size:
4777 1203 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4003 4764 5792 1540
Size: 275 186 2002 761 1028 2301
Fragments arranged by size:
2301 2002 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2941 4614 4699 5770 2941
Size: 1673 85 1071 3724
Fragments arranged by size:
3724 1673 1071 85
BsmI GAATG_Cn'
Cuts at: 2883 4064 4157 2883
Size: 1181 93 5279
Fragments arranged by size:
5279 1181 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2153 2153
Size: 6553
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2667 2941 3057 3189 3392 3872 4614
Size: 399 877 274 116 132 203 480 742
Cuts at: 4614 4699 5770 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 877 742 480 399 274 203
132 116 85
BspEI T'CCGG_A
Cuts at: 6216 6216
Size: 6553
BspHI T'CATG_A
Cuts at: 3112 4442 6172 3112
Size: 1330 1730 3493
Fragments arranged by size:
3493 1730 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3449 3739 3908 844
Size: 2605 290 169 3489
Fragments arranged by size:
3489 2605 290 169
BsrBI CCG'CTC
Cuts at: 1394 1823 2908 4440 1394
Size: 429 1085 1532 3507
Fragments arranged by size:
3507 1532 1085 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2040 2865 5048 5222 65
Size: 1183 792 825 2183 174 1396
Fragments arranged by size:
2183 1396 1183 825 792 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5197 5384 1700
Size: 110 3387 187 2869
Fragments arranged by size:
3387 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6553
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6553
BssSI C'ACGA_G
Cuts at: 3120 3264 4613 5625 3120
Size: 144 1349 1012 4048
Fragments arranged by size:
4048 1349 1012 144
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6553
BstBI TT'CG_AA
Cuts at: 5357 5357
Size: 6553
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6553
BstXI CCAn_nnnn'nTGG
Cuts at: 3346 3346
Size: 6553
BstYI r'GATC_y
Cuts at: 1972 2353 2551 2929 3163 3883 4250 4635 4652
Size: 381 198 378 234 720 367 385 17
Cuts at: 4652 6104 6190 6202 6548 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 720 385 381 378 367 346
234 198 86 17 12
BstZ17I GTA'TAC
Cuts at: 2149 3617 3632 3647 2149
Size: 1468 15 15 5055
Fragments arranged by size:
5055 1468 15 15
Bsu36I CC'TnA_GG
Cuts at: 3471 3471
Size: 6553
BtgI C'CryG_G
Cuts at: 513 1065 3270 513
Size: 552 2205 3796
Fragments arranged by size:
3796 2205 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3269 3344 487
Size: 683 56 2043 75 3696
Fragments arranged by size:
3696 2043 683 75 56
BtsI GCAGTG_nn'
Cuts at: 2045 4069 4863 4883 2045
Size: 2024 794 20 3715
Fragments arranged by size:
3715 2024 794 20
ClaI AT'CG_AT
Cuts at: 1554 2041 2361 2384 1554
Size: 487 320 23 5723
Fragments arranged by size:
5723 487 320 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3649 3684 529
Size: 35 3085 35 3398
Fragments arranged by size:
3398 3085 35 35
DraI TTT'AAA
Cuts at: 3819 3857 3819
Size: 38 6515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5560 809
Size: 507 4244 1802
Fragments arranged by size:
4244 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4000 4889 6518
Size: 54 1425 50 275 18 2170 889 1629
Cuts at: 6518 8
Size: 43
Fragments arranged by size:
2170 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4000 1537
Size: 275 2188 4090
Fragments arranged by size:
4090 2188 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4483 1353
Size: 3130 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5140 5514 5660 387
Size: 1064 138 3551 374 146 1280
Fragments arranged by size:
3551 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2939 2939
Size: 6553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2691 2781 2871 3063 3537 3864 4616
Size: 393 1059 90 90 192 474 327 752
Cuts at: 4616 5213 6000 6268 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1059 787 752 597 474 393 327
268 192 90 90
EcoNI CCTnn'n_nnAGG
Cuts at: 2982 2982
Size: 6553
EcoO109I rG'GnC_Cy
Cuts at: 2675 3086 3194 3388 3389 3410 3434 3476 3487
Size: 411 108 194 1 21 24 42 11
Cuts at: 3487 2675
Size: 5741
Fragments arranged by size:
5741 411 194 108 42 24 21 11
1
EcoRI G'AATT_C
Cuts at: 2787 3426 3862 4240 2787
Size: 639 436 378 5100
Fragments arranged by size:
5100 639 436 378
FspI TGC'GCA
Cuts at: 5059 5059
Size: 6553
HaeII r_GCGC'y
Cuts at: 1678 2264 2330 5700 1678
Size: 586 66 3370 2531
Fragments arranged by size:
3370 2531 586 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2755 2787 2914
Size: 32 135 32 524 32 627 32 127
Cuts at: 2914 2946 3247 3279 3754 3786 5194 5226 5268
Size: 32 301 32 475 32 1408 32 42
Cuts at: 5268 5300 1373
Size: 32 2626
Fragments arranged by size:
2626 1408 627 524 475 301 135 127
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3897 4143 669
Size: 988 281 1959 246 3079
Fragments arranged by size:
3079 1959 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6553
HpaI GTT'AAC
Cuts at: 4143 4143
Size: 6553
KpnI G_GTAC'C
Cuts at: 3878 3878
Size: 6553
MfeI C'AATT_G
Cuts at: 4152 4152
Size: 6553
MluI A'CGCG_T
Cuts at: 6429 6429
Size: 6553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5667 5851 1305
Size: 4362 184 2007
Fragments arranged by size:
4362 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4731
Fragments arranged by size:
4731 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4511 4870 5029 518
Size: 648 3345 359 159 2042
Fragments arranged by size:
3345 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2813 3969 4646 5794 6039 1606
Size: 1207 1156 677 1148 245 2120
Fragments arranged by size:
2120 1207 1156 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3270 513
Size: 552 2205 3796
Fragments arranged by size:
3796 2205 552
NdeI CA'TA_TG
Cuts at: 387 2077 387
Size: 1690 4863
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6553
NotI GC'GGCC_GC
Cuts at: 4000 4000
Size: 6553
NsiI A_TGCA'T
Cuts at: 3099 3099
Size: 6553
NspI r_CATG'y
Cuts at: 1132 1229 2488 2594 3559 3911 5456 1132
Size: 97 1259 106 965 352 1545 2229
Fragments arranged by size:
2229 1545 1259 965 352 106 97
PasI CC'CwG_GG
Cuts at: 1348 2660 1348
Size: 1312 5241
PciI A'CATG_T
Cuts at: 2484 2590 5452 2484
Size: 106 2862 3585
Fragments arranged by size:
3585 2862 106
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2888 1108
Size: 1780 4773
PfoI T'CCnGG_A
Cuts at: 2821 2848 3514 2821
Size: 27 666 5860
Fragments arranged by size:
5860 666 27
PmeI GTTT'AAAC
Cuts at: 3857 3857
Size: 6553
PmlI CAC'GTG
Cuts at: 3017 3296 3017
Size: 279 6274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4622 4654 6135 6167 4622
Size: 32 1481 32 5008
Fragments arranged by size:
5008 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2675 3086 3434 3476 3487 2675
Size: 411 348 42 11 5741
Fragments arranged by size:
5741 411 348 42 11
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6553
PsiI TTA'TAA
Cuts at: 4123 4123
Size: 6553
PspOMI G'GGCC_C
Cuts at: 3388 3388
Size: 6553
PspXI vC'TCGA_Gb
Cuts at: 2982 2982
Size: 6553
PstI C_TGCA'G
Cuts at: 830 2326 2818 3769 3905 830
Size: 1496 492 951 136 3478
Fragments arranged by size:
3478 1496 951 492 136
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6553
PvuII CAG'CTG
Cuts at: 2813 2813
Size: 6553
SacI G_AGCT'C
Cuts at: 2941 2941
Size: 6553
SalI G'TCGA_C
Cuts at: 1936 3895 1936
Size: 1959 4594
SanDI GG'GwC_CC
Cuts at: 3086 3476 3086
Size: 390 6163
SbfI CC_TGCA'GG
Cuts at: 3905 3905
Size: 6553
ScaI AGT'ACT
Cuts at: 4801 6513 4801
Size: 1712 4841
SfcI C'TryA_G
Cuts at: 826 1045 2322 2814 3765 3901 5036 5717 5908
Size: 219 1277 492 951 136 1135 681 191
Cuts at: 5908 826
Size: 1471
Fragments arranged by size:
1471 1277 1135 951 681 492 219 191
136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6553
SmaI CCC'GGG
Cuts at: 1872 3880 1872
Size: 2008 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2982 3249 4657 5558 5820 820
Size: 197 926 1039 267 1408 901 262 1553
Fragments arranged by size:
1553 1408 1039 926 901 267 262 197
SnaBI TAC'GTA
Cuts at: 493 4344 493
Size: 3851 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6553
SphI G_CATG'C
Cuts at: 1132 3559 3911 1132
Size: 2427 352 3774
Fragments arranged by size:
3774 2427 352
SspI AAT'ATT
Cuts at: 5 52 4477 5
Size: 47 4425 2081
Fragments arranged by size:
4425 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6553
StyI C'CwwG_G
Cuts at: 513 1065 2172 2314 3270 3992 513
Size: 552 1107 142 956 722 3074
Fragments arranged by size:
3074 1107 956 722 552 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4580 4597 4750 4935 5380 325
Size: 1255 3000 17 153 185 445 1498
Fragments arranged by size:
3000 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4799 6511 96
Size: 274 80 33 51 1443 2822 1712 138
Fragments arranged by size:
2822 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2589 2621 6513 6545 2589
Size: 32 3892 32 2597
Fragments arranged by size:
3892 2597 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6553
XbaI T'CTAG_A
Cuts at: 3889 3889
Size: 6553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3064 3839 2011
Size: 1053 775 4725
Fragments arranged by size:
4725 1053 775
XhoI C'TCGA_G
Cuts at: 1943 2982 3249 1943
Size: 1039 267 5247
Fragments arranged by size:
5247 1039 267
XmaI C'CCGG_G
Cuts at: 1870 3878 1870
Size: 2008 4545
XmnI GAAnn'nnTTC
Cuts at: 4682 4682
Size: 6553
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4361 276
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BplI BsiWI DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PsrI RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI