Restriction map for
pFN21AE5783
(Circular) (Six-base) MAPSORT of: pFN21AE5783.seq Check: 1609 from: 1 to: 6748
pFN21AE5783 6748 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3226 3226
Size: 6748
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2220 4558 278
Size: 53 83 186 936 684 2338 2468
Fragments arranged by size:
2468 2338 936 684 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3343 4091 1937
Size: 1406 748 4594
Fragments arranged by size:
4594 1406 748
Acc65I G'GTAC_C
Cuts at: 4069 4069
Size: 6748
AclI AA'CG_TT
Cuts at: 1563 4875 5248 1563
Size: 3312 373 3063
Fragments arranged by size:
3312 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2697 3042 3193 3246 4811 6195 6463 1632
Size: 1065 345 151 53 1565 1384 268 1917
Fragments arranged by size:
1917 1565 1384 1065 345 268 151 53
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6551
AflIII A'CryG_T
Cuts at: 1203 5647 6624 1203
Size: 4444 977 1327
Fragments arranged by size:
4444 1327 977
AgeI A'CCGG_T
Cuts at: 5579 5579
Size: 6748
AhdI GACnn_n'nnGTC
Cuts at: 5477 5477
Size: 6748
AleI CACnn'nnGTG
Cuts at: 1166 3523 1166
Size: 2357 4391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6748
AlwNI CAG_nnn'CTG
Cuts at: 3224 3241 3689 3713 3922 4491 6063 3224
Size: 17 448 24 209 569 1572 3909
Fragments arranged by size:
3909 1572 569 448 209 24 17
ApaLI G'TGCA_C
Cuts at: 4805 5961 4805
Size: 1156 5592
ApoI r'AATT_y
Cuts at: 1493 2429 2500 3948 4057 4283 4435 6544 1493
Size: 936 71 1448 109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 936 226 152 109 71
AseI AT'TA_AT
Cuts at: 160 1032 5302 160
Size: 872 4270 1606
Fragments arranged by size:
4270 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6748
AvaI C'yCGr_G
Cuts at: 1870 1943 4073 1870
Size: 73 2130 4545
Fragments arranged by size:
4545 2130 73
BamHI G'GATC_C
Cuts at: 4078 4445 4078
Size: 367 6381
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4069 5524 618
Size: 325 217 627 2282 1455 1842
Fragments arranged by size:
2282 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3003 4067 1391
Size: 1612 1064 4072
Fragments arranged by size:
4072 1612 1064
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2703 3350 928
Size: 971 804 647 4326
Fragments arranged by size:
4326 971 804 647
BbvCI CC'TCA_GC
Cuts at: 3306 3579 3306
Size: 273 6475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4939 4973 1903
Size: 34 3002 34 3678
Fragments arranged by size:
3678 3002 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4640 5850 4640
Size: 1210 5538
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6748
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2063 5359
Size: 107 122 71 631 605 337 54 3296
Cuts at: 5359 136
Size: 1525
Fragments arranged by size:
3296 1525 631 605 337 122 107 71
54
BglII A'GATC_T
Cuts at: 6743 6743
Size: 6748
BlpI GC'TnA_GC
Cuts at: 4165 4165
Size: 6748
Bme1580I G_kGCm'C
Cuts at: 1790 2692 3321 3611 4809 5965 1790
Size: 902 629 290 1198 1156 2573
Fragments arranged by size:
2573 1198 1156 902 629 290
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6748
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2950 3072 4479
Size: 416 53 83 399 24 1533 122 1407
Cuts at: 4479 5437 442
Size: 958 1753
Fragments arranged by size:
1753 1533 1407 958 416 399 122 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3957 5408 1239
Size: 2718 1451 2579
Fragments arranged by size:
2718 2579 1451
Bpu10I CC'TnA_GC
Cuts at: 2787 3306 3579 2787
Size: 519 273 5956
Fragments arranged by size:
5956 519 273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2526 4873 5738 6036 2526
Size: 2347 865 298 3238
Fragments arranged by size:
3238 2347 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2835 5411 882
Size: 617 1336 2576 2219
Fragments arranged by size:
2576 2219 1336 617
BsaAI yAC'GTr
Cuts at: 493 1204 4539 493
Size: 711 3335 2702
Fragments arranged by size:
3335 2702 711
BsaBI GATnn'nnATC
Cuts at: 6742 6742
Size: 6748
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2217 2237 4555 4937
Size: 53 83 186 936 684 20 2318 382
Cuts at: 4937 5572 275
Size: 635 1451
Fragments arranged by size:
2318 1451 936 684 635 382 186 83
53 20
BsaWI w'CCGG_w
Cuts at: 2017 2167 5181 5579 5853 6000 6411 2017
Size: 150 3014 398 274 147 411 2354
Fragments arranged by size:
3014 2354 411 398 274 150 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1999 2029 1178
Size: 30 165 30 596 30 5897
Fragments arranged by size:
5897 596 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2846 3244 3596 3654 1186
Size: 1660 398 352 58 4280
Fragments arranged by size:
4280 1660 398 352 58
BseYI C'CCAG_C
Cuts at: 2051 3778 3821 5951 6447 2051
Size: 1727 43 2130 496 2352
Fragments arranged by size:
2352 2130 1727 496 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2787 3501 1163
Size: 573 1051 714 4410
Fragments arranged by size:
4410 1051 714 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4198 4959 5987 1540
Size: 275 186 2197 761 1028 2301
Fragments arranged by size:
2301 2197 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2838 3218 3754 4809 4894 5965 2838
Size: 380 536 1055 85 1071 3621
Fragments arranged by size:
3621 1071 1055 536 380 85
BsmI GAATG_Cn'
Cuts at: 2402 2788 3163 3918 4259 4352 2402
Size: 386 375 755 341 93 4798
Fragments arranged by size:
4798 755 386 375 341 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2692 2838 3003 3218 3321 3611 3754
Size: 399 902 146 165 215 103 290 143
Cuts at: 3754 4067 4809 4894 5965 1391
Size: 313 742 85 1071 2174
Fragments arranged by size:
2174 1071 902 742 399 313 290 215
165 146 143 103 85
BspEI T'CCGG_A
Cuts at: 2017 6411 2017
Size: 4394 2354
BspHI T'CATG_A
Cuts at: 3798 4637 6367 3798
Size: 839 1730 4179
Fragments arranged by size:
4179 1730 839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3226 4103 844
Size: 2382 877 3489
Fragments arranged by size:
3489 2382 877
BsrBI CCG'CTC
Cuts at: 1394 1823 4635 1394
Size: 429 2812 3507
Fragments arranged by size:
3507 2812 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2290 2580 3049 5243 5417 65
Size: 1183 1042 290 469 2194 174 1396
Fragments arranged by size:
2194 1396 1183 1042 469 290 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5392 5579 1700
Size: 110 3582 187 2869
Fragments arranged by size:
3582 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6748
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6748
BssSI C'ACGA_G
Cuts at: 2691 3747 4808 5820 2691
Size: 1056 1061 1012 3619
Fragments arranged by size:
3619 1061 1056 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 2356 3158 3316 794
Size: 1361 201 802 158 4226
Fragments arranged by size:
4226 1361 802 201 158
BstBI TT'CG_AA
Cuts at: 2422 5552 2422
Size: 3130 3618
BstEII G'GTnAC_C
Cuts at: 1184 3968 1184
Size: 2784 3964
BstYI r'GATC_y
Cuts at: 1972 2119 2608 4078 4445 4830 4847 6299 6385
Size: 147 489 1470 367 385 17 1452 86
Cuts at: 6385 6397 6743 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1470 1452 489 385 367 346 147
86 17 12
BstZ17I GTA'TAC
Cuts at: 3344 3344
Size: 6748
Bsu36I CC'TnA_GG
Cuts at: 2364 2364
Size: 6748
BtgI C'CryG_G
Cuts at: 513 1065 2702 513
Size: 552 1637 4559
Fragments arranged by size:
4559 1637 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6009
Fragments arranged by size:
6009 683 56
BtsI GCAGTG_nn'
Cuts at: 2046 3752 4264 5058 5078 2046
Size: 1706 512 794 20 3716
Fragments arranged by size:
3716 1706 794 512 20
ClaI AT'CG_AT
Cuts at: 1554 3247 1554
Size: 1693 5055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6713
DraI TTT'AAA
Cuts at: 2499 3615 4052 2499
Size: 1116 437 5195
Fragments arranged by size:
5195 1116 437
DraIII CAC_nnn'GTG
Cuts at: 2155 2155
Size: 6748
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3125 5755 809
Size: 507 1809 2630 1802
Fragments arranged by size:
2630 1809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 3012 4195
Size: 54 1425 50 275 18 294 888 1183
Cuts at: 4195 5084 6713 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1183 889 888 294 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4195 1537
Size: 275 2383 4090
Fragments arranged by size:
4090 2383 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2504 3212 3800 4678 1353
Size: 1151 708 588 878 3423
Fragments arranged by size:
3423 1151 878 708 588
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5335 5709 5855 387
Size: 1064 138 3746 374 146 1280
Fragments arranged by size:
3746 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2697 3042 3193 3246 3957 4811 5408
Size: 393 1065 345 151 53 711 854 597
Cuts at: 5408 6195 6463 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1065 854 787 711 597 393 345
268 151 53
EcoO109I rG'GnC_Cy
Cuts at: 2877 3919 2877
Size: 1042 5706
EcoRI G'AATT_C
Cuts at: 2429 4057 4435 2429
Size: 1628 378 4742
Fragments arranged by size:
4742 1628 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2661 2936 2661
Size: 275 6473
FspI TGC'GCA
Cuts at: 5254 5254
Size: 6748
HaeII r_GCGC'y
Cuts at: 1678 5895 1678
Size: 4217 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 3727 3759 5389
Size: 32 135 32 427 32 1696 32 1630
Cuts at: 5389 5421 5463 5495 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1696 1630 427 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4092 4338 669
Size: 988 281 2154 246 3079
Fragments arranged by size:
3079 2154 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6748
HpaI GTT'AAC
Cuts at: 4338 4338
Size: 6748
KpnI G_GTAC'C
Cuts at: 4073 4073
Size: 6748
MfeI C'AATT_G
Cuts at: 3375 4347 3375
Size: 972 5776
MluI A'CGCG_T
Cuts at: 6624 6624
Size: 6748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2157 2284 3302 5862 6046 1305
Size: 852 127 1018 2560 184 2007
Fragments arranged by size:
2560 2007 1018 852 184 127
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 10
Size: 54 1425 343 294 4632
Fragments arranged by size:
4632 1425 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3523 3640 4005 4706 5065 5224 518
Size: 648 2357 117 365 701 359 159 2042
Fragments arranged by size:
2357 2042 701 648 365 359 159 117
MspA1I CmG'CkG
Cuts at: 1606 2245 3020 3224 3578 3866 4164 4841 5989
Size: 639 775 204 354 288 298 677 1148
Cuts at: 5989 6234 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 775 677 639 354 298 288
245 204
NcoI C'CATG_G
Cuts at: 513 1065 2702 513
Size: 552 1637 4559
Fragments arranged by size:
4559 1637 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6748
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6748
NotI GC'GGCC_GC
Cuts at: 4195 4195
Size: 6748
NspI r_CATG'y
Cuts at: 1132 1229 2995 4106 5651 1132
Size: 97 1766 1111 1545 2229
Fragments arranged by size:
2229 1766 1545 1111 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6748
PciI A'CATG_T
Cuts at: 5647 5647
Size: 6748
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3896 4035 1108
Size: 2788 139 3821
Fragments arranged by size:
3821 2788 139
PfoI T'CCnGG_A
Cuts at: 3387 3387
Size: 6748
PmeI GTTT'AAAC
Cuts at: 4052 4052
Size: 6748
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4817 4849 6330 6362 4817
Size: 32 1481 32 5203
Fragments arranged by size:
5203 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2877 2877
Size: 6748
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6748
PsiI TTA'TAA
Cuts at: 2592 4318 2592
Size: 1726 5022
PstI C_TGCA'G
Cuts at: 830 3697 3759 4100 830
Size: 2867 62 341 3478
Fragments arranged by size:
3478 2867 341 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6748
PvuII CAG'CTG
Cuts at: 2245 3020 3224 3578 3866 2245
Size: 775 204 354 288 5127
Fragments arranged by size:
5127 775 354 288 204
SalI G'TCGA_C
Cuts at: 1936 4090 1936
Size: 2154 4594
SanDI GG'GwC_CC
Cuts at: 2877 2877
Size: 6748
SapI GCTCTTCn'nnn_
Cuts at: 3800 3800
Size: 6748
SbfI CC_TGCA'GG
Cuts at: 4100 4100
Size: 6748
ScaI AGT'ACT
Cuts at: 3300 4996 6708 3300
Size: 1696 1712 3340
Fragments arranged by size:
3340 1712 1696
SfcI C'TryA_G
Cuts at: 826 1045 2852 3693 3705 3755 3861 4096 5231
Size: 219 1807 841 12 50 106 235 1135
Cuts at: 5231 5912 6103 826
Size: 681 191 1471
Fragments arranged by size:
1807 1471 1135 841 681 235 219 191
106 50 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2063 2063
Size: 6748
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6748
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6748
SmaI CCC'GGG
Cuts at: 1872 4075 1872
Size: 2203 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2541 4852 5753 6015 820
Size: 197 926 598 2311 901 262 1553
Fragments arranged by size:
2311 1553 926 901 598 262 197
SnaBI TAC'GTA
Cuts at: 493 4539 493
Size: 4046 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6748
SphI G_CATG'C
Cuts at: 1132 2995 4106 1132
Size: 1863 1111 3774
Fragments arranged by size:
3774 1863 1111
SspI AAT'ATT
Cuts at: 5 52 2296 2752 4672 5
Size: 47 2244 456 1920 2081
Fragments arranged by size:
2244 2081 1920 456 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6748
StyI C'CwwG_G
Cuts at: 513 1065 2702 2757 2967 3964 4187 513
Size: 552 1637 55 210 997 223 3074
Fragments arranged by size:
3074 1637 997 552 223 210 55
SwaI ATTT'AAAT
Cuts at: 2499 2499
Size: 6748
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4775 4792 4945 5130 5575 325
Size: 1255 3195 17 153 185 445 1498
Fragments arranged by size:
3195 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2324 2383 3298
Size: 274 80 33 51 1443 347 59 915
Cuts at: 3298 4994 6706 96
Size: 1696 1712 138
Fragments arranged by size:
1712 1696 1443 915 347 274 138 80
59 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6708 6740 6708
Size: 32 6716
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6748
XbaI T'CTAG_A
Cuts at: 4084 4084
Size: 6748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2134 2134
Size: 6748
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6748
XmaI C'CCGG_G
Cuts at: 1870 4073 1870
Size: 2203 4545
XmnI GAAnn'nnTTC
Cuts at: 3248 3564 4877 3248
Size: 316 1313 5119
Fragments arranged by size:
5119 1313 316
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2218 4556 276
Size: 53 83 186 936 684 2338 2468
Fragments arranged by size:
2468 2338 936 684 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI
SfoI SrfI