Restriction map for
pFN21AE5779
(Circular) (Six-base) MAPSORT of: pFN21AE5779.seq Check: 6703 from: 1 to: 6640
pFN21AE5779 6640 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4450 278
Size: 53 83 186 936 2914 2468
Fragments arranged by size:
2914 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2868 3983 1937
Size: 931 1115 4594
Fragments arranged by size:
4594 1115 931
Acc65I G'GTAC_C
Cuts at: 3961 3961
Size: 6640
AclI AA'CG_TT
Cuts at: 1563 4767 5140 1563
Size: 3204 373 3063
Fragments arranged by size:
3204 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3144 3902 4703 6087 6355 1632
Size: 1512 758 801 1384 268 1917
Fragments arranged by size:
1917 1512 1384 801 758 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6443
AflIII A'CryG_T
Cuts at: 1203 2162 5539 6516 1203
Size: 959 3377 977 1327
Fragments arranged by size:
3377 1327 977 959
AgeI A'CCGG_T
Cuts at: 5471 5471
Size: 6640
AhdI GACnn_n'nnGTC
Cuts at: 5369 5369
Size: 6640
AleI CACnn'nnGTG
Cuts at: 1166 3531 1166
Size: 2365 4275
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2483 1184
Size: 1299 5341
AlwNI CAG_nnn'CTG
Cuts at: 2309 3863 4383 5955 2309
Size: 1554 520 1572 2994
Fragments arranged by size:
2994 1572 1554 520
ApaLI G'TGCA_C
Cuts at: 4697 5853 4697
Size: 1156 5484
ApoI r'AATT_y
Cuts at: 1493 2124 2132 3235 3630 3949 4175 4327 6436
Size: 631 8 1103 395 319 226 152 2109
Cuts at: 6436 1493
Size: 1697
Fragments arranged by size:
2109 1697 1103 631 395 319 226 152
8
AseI AT'TA_AT
Cuts at: 160 1032 3281 5194 160
Size: 872 2249 1913 1606
Fragments arranged by size:
2249 1913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6640
AvaI C'yCGr_G
Cuts at: 1870 1943 3965 1870
Size: 73 2022 4545
Fragments arranged by size:
4545 2022 73
BamHI G'GATC_C
Cuts at: 2880 3970 4337 2880
Size: 1090 367 5183
Fragments arranged by size:
5183 1090 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2669 3727 3961 5416 618
Size: 325 217 627 882 1058 234 1455 1842
Fragments arranged by size:
1842 1455 1058 882 627 325 234 217
BanII G_rGCy'C
Cuts at: 1391 3561 3937 3959 1391
Size: 2170 376 22 4072
Fragments arranged by size:
4072 2170 376 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5669
BbvCI CC'TCA_GC
Cuts at: 2970 2970
Size: 6640
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4831 4865 1903
Size: 34 2894 34 3678
Fragments arranged by size:
3678 2894 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3128 4532 5742 3128
Size: 1404 1210 4026
Fragments arranged by size:
4026 1404 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6640
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5251 136
Size: 107 122 71 631 605 3579 1525
Fragments arranged by size:
3579 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6635 6635
Size: 6640
BlpI GC'TnA_GC
Cuts at: 2442 4057 2442
Size: 1615 5025
Bme1580I G_kGCm'C
Cuts at: 1790 3730 4701 5857 1790
Size: 1940 971 1156 2573
Fragments arranged by size:
2573 1940 1156 971
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6640
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2886 3525 4371
Size: 416 53 83 399 24 1469 639 846
Cuts at: 4371 5329 442
Size: 958 1753
Fragments arranged by size:
1753 1469 958 846 639 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6640
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3428
Size: 6640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2891 3225 3351 3692 5300 1239
Size: 1652 334 126 341 1608 2579
Fragments arranged by size:
2579 1652 1608 341 334 126
Bpu10I CC'TnA_GC
Cuts at: 2970 2970
Size: 6640
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4765 5630 5928 4765
Size: 865 298 5477
Fragments arranged by size:
5477 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3290 5303 882
Size: 617 1791 2013 2219
Fragments arranged by size:
2219 2013 1791 617
BsaAI yAC'GTr
Cuts at: 493 1204 4431 493
Size: 711 3227 2702
Fragments arranged by size:
3227 2702 711
BsaBI GATnn'nnATC
Cuts at: 2423 6634 2423
Size: 4211 2429
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4447 4829 5464 275
Size: 53 83 186 936 2914 382 635 1451
Fragments arranged by size:
2914 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2151 5073 5471 5745 5892 6303 2151
Size: 2922 398 274 147 411 2488
Fragments arranged by size:
2922 2488 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3772 3802 1178
Size: 30 165 30 2369 30 4016
Fragments arranged by size:
4016 2369 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2134 3417 1186
Size: 948 1283 4409
Fragments arranged by size:
4409 1283 948
BseYI C'CCAG_C
Cuts at: 5843 6339 5843
Size: 496 6144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6067
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4090 4851 5879 1540
Size: 275 186 2089 761 1028 2301
Fragments arranged by size:
2301 2089 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3339 3414 3937 4701 4786 5857 3339
Size: 75 523 764 85 1071 4122
Fragments arranged by size:
4122 1071 764 523 85 75
BsmI GAATG_Cn'
Cuts at: 2458 4151 4244 2458
Size: 1693 93 4854
Fragments arranged by size:
4854 1693 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2187 2187
Size: 6640
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3339 3414 3561 3730 3937 3959 4701
Size: 399 1549 75 147 169 207 22 742
Cuts at: 4701 4786 5857 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1549 1071 742 399 207 169 147
85 75 22
BspEI T'CCGG_A
Cuts at: 2151 6303 2151
Size: 4152 2488
BspHI T'CATG_A
Cuts at: 2586 3843 4529 6259 2586
Size: 1257 686 1730 2967
Fragments arranged by size:
2967 1730 1257 686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2571 3995 844
Size: 1727 1424 3489
Fragments arranged by size:
3489 1727 1424
BsrBI CCG'CTC
Cuts at: 1394 1823 4527 1394
Size: 429 2704 3507
Fragments arranged by size:
3507 2704 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3610 5135 5309 65
Size: 1183 2362 1525 174 1396
Fragments arranged by size:
2362 1525 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5284 5471 1700
Size: 110 3474 187 2869
Fragments arranged by size:
3474 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2034 3549 96
Size: 1938 1515 3187
Fragments arranged by size:
3187 1938 1515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6640
BssSI C'ACGA_G
Cuts at: 4700 5712 4700
Size: 1012 5628
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6640
BstBI TT'CG_AA
Cuts at: 3628 5444 3628
Size: 1816 4824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6640
BstXI CCAn_nnnn'nTGG
Cuts at: 3493 3493
Size: 6640
BstYI r'GATC_y
Cuts at: 1972 2880 2932 3785 3970 4337 4722 4739 6191
Size: 908 52 853 185 367 385 17 1452
Cuts at: 6191 6277 6289 6635 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 908 853 385 367 346 185
86 52 17 12
Bsu36I CC'TnA_GG
Cuts at: 3751 3873 3751
Size: 122 6518
BtgI C'CryG_G
Cuts at: 513 1065 3094 513
Size: 552 2029 4059
Fragments arranged by size:
4059 2029 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5901
Fragments arranged by size:
5901 683 56
BtsI GCAGTG_nn'
Cuts at: 4156 4950 4970 4156
Size: 794 20 5826
Fragments arranged by size:
5826 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6640
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2146 2181 3387 3422 529
Size: 35 1582 35 1206 35 3747
Fragments arranged by size:
3747 1582 1206 35 35 35
DraI TTT'AAA
Cuts at: 3597 3944 3597
Size: 347 6293
DraIII CAC_nnn'GTG
Cuts at: 2208 2208
Size: 6640
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5647 809
Size: 507 4331 1802
Fragments arranged by size:
4331 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2979 4087 4976
Size: 54 1425 50 275 18 1149 1108 889
Cuts at: 4976 6605 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1149 1108 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4087 1537
Size: 275 2275 4090
Fragments arranged by size:
4090 2275 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2514 3467 4570 1353
Size: 1161 953 1103 3423
Fragments arranged by size:
3423 1161 1103 953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5227 5601 5747 387
Size: 1064 138 3638 374 146 1280
Fragments arranged by size:
3638 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3935 3935
Size: 6640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2891 3144 3225 3351 3692 3902 4703
Size: 393 1259 253 81 126 341 210 801
Cuts at: 4703 5300 6087 6355 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1259 801 787 597 393 341 268
253 210 126 81
EcoNI CCTnn'n_nnAGG
Cuts at: 3470 3738 3470
Size: 268 6372
EcoO109I rG'GnC_Cy
Cuts at: 2574 2658 2574
Size: 84 6556
EcoRI G'AATT_C
Cuts at: 2124 2132 3235 3630 3949 4327 2124
Size: 8 1103 395 319 378 4437
Fragments arranged by size:
4437 1103 395 378 319 8
EcoRV GAT'ATC
Cuts at: 3034 3034
Size: 6640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 3098 2488
Size: 610 6030
FspI TGC'GCA
Cuts at: 5146 5146
Size: 6640
HaeII r_GCGC'y
Cuts at: 1678 5787 1678
Size: 4109 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3143 3175 3482 3514 3825
Size: 32 135 32 1571 32 307 32 311
Cuts at: 3825 3857 5281 5313 5355 5387 1373
Size: 32 1424 32 42 32 2626
Fragments arranged by size:
2626 1571 1424 311 307 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3984 4230 669
Size: 988 281 2046 246 3079
Fragments arranged by size:
3079 2046 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6640
HpaI GTT'AAC
Cuts at: 4230 4230
Size: 6640
KpnI G_GTAC'C
Cuts at: 3965 3965
Size: 6640
MfeI C'AATT_G
Cuts at: 4239 4239
Size: 6640
MluI A'CGCG_T
Cuts at: 6516 6516
Size: 6640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5754 5938 1305
Size: 4449 184 2007
Fragments arranged by size:
4449 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2981 10
Size: 54 1425 343 1149 3669
Fragments arranged by size:
3669 1425 1149 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2591 2855 3531 4598 4957 5116 518
Size: 648 1425 264 676 1067 359 159 2042
Fragments arranged by size:
2042 1425 1067 676 648 359 264 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2441 3179 4056 4733 5881 6126 1606
Size: 426 409 738 877 677 1148 245 2120
Fragments arranged by size:
2120 1148 877 738 677 426 409 245
NcoI C'CATG_G
Cuts at: 513 1065 3094 513
Size: 552 2029 4059
Fragments arranged by size:
4059 2029 552
NdeI CA'TA_TG
Cuts at: 387 3113 387
Size: 2726 3914
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6640
NotI GC'GGCC_GC
Cuts at: 4087 4087
Size: 6640
NspI r_CATG'y
Cuts at: 1132 1229 2166 3351 3998 5543 1132
Size: 97 937 1185 647 1545 2229
Fragments arranged by size:
2229 1545 1185 937 647 97
PasI CC'CwG_GG
Cuts at: 1348 2663 1348
Size: 1315 5325
PciI A'CATG_T
Cuts at: 2162 5539 2162
Size: 3377 3263
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6640
PmeI GTTT'AAAC
Cuts at: 3944 3944
Size: 6640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4709 4741 6222 6254 4709
Size: 32 1481 32 5095
Fragments arranged by size:
5095 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2574 2658 2574
Size: 84 6556
PshAI GACnn'nnGTC
Cuts at: 1613 2572 1613
Size: 959 5681
PsiI TTA'TAA
Cuts at: 4210 4210
Size: 6640
PstI C_TGCA'G
Cuts at: 830 2386 3651 3992 830
Size: 1556 1265 341 3478
Fragments arranged by size:
3478 1556 1265 341
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6640
PvuII CAG'CTG
Cuts at: 2032 2441 3179 2032
Size: 409 738 5493
Fragments arranged by size:
5493 738 409
SacI G_AGCT'C
Cuts at: 3937 3937
Size: 6640
SalI G'TCGA_C
Cuts at: 1936 3982 1936
Size: 2046 4594
SapI GCTCTTCn'nnn_
Cuts at: 2514 2514
Size: 6640
SbfI CC_TGCA'GG
Cuts at: 2386 3992 2386
Size: 1606 5034
ScaI AGT'ACT
Cuts at: 4888 6600 4888
Size: 1712 4928
SfcI C'TryA_G
Cuts at: 826 1045 2092 2382 3647 3988 5123 5804 5995
Size: 219 1047 290 1265 341 1135 681 191
Cuts at: 5995 826
Size: 1471
Fragments arranged by size:
1471 1265 1135 1047 681 341 290 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6640
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6640
SmaI CCC'GGG
Cuts at: 1872 3967 1872
Size: 2095 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4744 5645 5907 820
Size: 197 926 2801 901 262 1553
Fragments arranged by size:
2801 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4431 493
Size: 3938 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6640
SphI G_CATG'C
Cuts at: 1132 3351 3998 1132
Size: 2219 647 3774
Fragments arranged by size:
3774 2219 647
SspI AAT'ATT
Cuts at: 5 52 4564 5
Size: 47 4512 2081
Fragments arranged by size:
4512 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6640
StyI C'CwwG_G
Cuts at: 513 1065 3094 3471 4079 513
Size: 552 2029 377 608 3074
Fragments arranged by size:
3074 2029 608 552 377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 3433 3525 4667 4684 4837 5022
Size: 1255 504 1349 92 1142 17 153 185
Cuts at: 5022 5467 325
Size: 445 1498
Fragments arranged by size:
1498 1349 1255 1142 504 445 185 153
92 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 2034 3549
Size: 274 80 33 51 1443 30 27 1515
Cuts at: 3549 4886 6598 96
Size: 1337 1712 138
Fragments arranged by size:
1712 1515 1443 1337 274 138 80 51
33 30 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3772 3804 6600 6632 3772
Size: 32 2796 32 3780
Fragments arranged by size:
3780 2796 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6640
XbaI T'CTAG_A
Cuts at: 3391 3976 3391
Size: 585 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6640
XmaI C'CCGG_G
Cuts at: 1870 3965 1870
Size: 2095 4545
XmnI GAAnn'nnTTC
Cuts at: 4769 4769
Size: 6640
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4448 276
Size: 53 83 186 936 2914 2468
Fragments arranged by size:
2914 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI