Restriction map for
pFN21AE5778
(Circular) (Six-base) MAPSORT of: pFN21AE5778.seq Check: 545 from: 1 to: 6580
pFN21AE5778 6580 bp
With 182 enzymes: SgfI *
June 20, 2019 11:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2096 3199 2096
Size: 1103 5477
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2436 4390 278
Size: 53 83 186 936 900 1954 2468
Fragments arranged by size:
2468 1954 936 900 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2500 2842 3336 3923 1937
Size: 563 342 494 587 4594
Fragments arranged by size:
4594 587 563 494 342
Acc65I G'GTAC_C
Cuts at: 3901 3901
Size: 6580
AclI AA'CG_TT
Cuts at: 1563 4707 5080 1563
Size: 3144 373 3063
Fragments arranged by size:
3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2031 2525 4643 6027 6295 1632
Size: 399 494 2118 1384 268 1917
Fragments arranged by size:
2118 1917 1384 494 399 268
AfeI AGC'GCT
Cuts at: 2329 2329
Size: 6580
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6383
AflIII A'CryG_T
Cuts at: 1203 5479 6456 1203
Size: 4276 977 1327
Fragments arranged by size:
4276 1327 977
AgeI A'CCGG_T
Cuts at: 5411 5411
Size: 6580
AhdI GACnn_n'nnGTC
Cuts at: 5309 5309
Size: 6580
AleI CACnn'nnGTG
Cuts at: 1166 3249 3833 1166
Size: 2083 584 3913
Fragments arranged by size:
3913 2083 584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6580
AlwNI CAG_nnn'CTG
Cuts at: 2054 2962 4323 5895 2054
Size: 908 1361 1572 2739
Fragments arranged by size:
2739 1572 1361 908
ApaLI G'TGCA_C
Cuts at: 2583 2965 4637 5793 2583
Size: 382 1672 1156 3370
Fragments arranged by size:
3370 1672 1156 382
ApoI r'AATT_y
Cuts at: 1493 3889 4115 4267 6376 1493
Size: 2396 226 152 2109 1697
Fragments arranged by size:
2396 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5134 160
Size: 872 4102 1606
Fragments arranged by size:
4102 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
AvaI C'yCGr_G
Cuts at: 1870 1943 3905 1870
Size: 73 1962 4545
Fragments arranged by size:
4545 1962 73
BamHI G'GATC_C
Cuts at: 2209 3910 4277 2209
Size: 1701 367 4512
Fragments arranged by size:
4512 1701 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3901 5356 618
Size: 325 217 627 2114 1455 1842
Fragments arranged by size:
2114 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3899 1391
Size: 2508 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2110 928
Size: 971 211 5398
Fragments arranged by size:
5398 971 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3483 3517 3782 3816 4771 4805 1903
Size: 34 1546 34 265 34 955 34 3678
Fragments arranged by size:
3678 1546 955 265 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3101 4472 5682 3101
Size: 1371 1210 3999
Fragments arranged by size:
3999 1371 1210
BclI T'GATC_A
Cuts at: 1548 3412 1548
Size: 1864 4716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5191 136
Size: 107 122 71 631 605 3519 1525
Fragments arranged by size:
3519 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6575 6575
Size: 6580
BlpI GC'TnA_GC
Cuts at: 3997 3997
Size: 6580
Bme1580I G_kGCm'C
Cuts at: 1790 2587 2969 4641 5797 1790
Size: 797 382 1672 1156 2573
Fragments arranged by size:
2573 1672 1156 797 382
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6580
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2793 2861 4311
Size: 416 53 83 399 24 1376 68 1450
Cuts at: 4311 5269 442
Size: 958 1753
Fragments arranged by size:
1753 1450 1376 958 416 399 83 68
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6580
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2841 2841
Size: 6580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 3584 3881 5240 1239
Size: 1262 1083 297 1359 2579
Fragments arranged by size:
2579 1359 1262 1083 297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2388 3855 4705 5570 5868 2388
Size: 1467 850 865 298 3100
Fragments arranged by size:
3100 1467 865 850 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5243 882
Size: 617 3744 2219
Fragments arranged by size:
3744 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2454 3847 4371 493
Size: 711 1250 1393 524 2702
Fragments arranged by size:
2702 1393 1250 711 524
BsaBI GATnn'nnATC
Cuts at: 6574 6574
Size: 6580
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2433 4387 4769 5404
Size: 53 83 186 936 900 1954 382 635
Cuts at: 5404 275
Size: 1451
Fragments arranged by size:
1954 1451 936 900 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5013 5411 5685 5832 6243 5013
Size: 398 274 147 411 5350
Fragments arranged by size:
5350 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6355
Fragments arranged by size:
6355 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3108 1186
Size: 1922 4658
BseYI C'CCAG_C
Cuts at: 2474 3004 3546 3786 5783 6279 2474
Size: 530 542 240 1997 496 2775
Fragments arranged by size:
2775 1997 542 530 496 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2137 2677 3203 1163
Size: 573 401 540 526 4540
Fragments arranged by size:
4540 573 540 526 401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4030 4791 5819 1540
Size: 275 186 2029 761 1028 2301
Fragments arranged by size:
2301 2029 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2404 2587 2969 3805 4641 4726 5797 2404
Size: 183 382 836 836 85 1071 3187
Fragments arranged by size:
3187 1071 836 836 382 183 85
BsiWI C'GTAC_G
Cuts at: 2454 2454
Size: 6580
BsmI GAATG_Cn'
Cuts at: 2414 4091 4184 2414
Size: 1677 93 4810
Fragments arranged by size:
4810 1677 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2404 2587 2969 3805 3899 4641 4726
Size: 399 614 183 382 836 94 742 85
Cuts at: 4726 5797 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 836 742 614 399 382 183
94 85
BspEI T'CCGG_A
Cuts at: 6243 6243
Size: 6580
BspHI T'CATG_A
Cuts at: 4469 6199 4469
Size: 1730 4850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2096 3199 3935 844
Size: 1252 1103 736 3489
Fragments arranged by size:
3489 1252 1103 736
BsrBI CCG'CTC
Cuts at: 1394 1823 3293 3814 4467 1394
Size: 429 1470 521 653 3507
Fragments arranged by size:
3507 1470 653 521 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2315 5075 5249 65
Size: 1183 1067 2760 174 1396
Fragments arranged by size:
2760 1396 1183 1067 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3686 5224 5411 1700
Size: 110 1876 1538 187 2869
Fragments arranged by size:
2869 1876 1538 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6580
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6580
BssSI C'ACGA_G
Cuts at: 2945 3781 4640 5652 2945
Size: 836 859 1012 3873
Fragments arranged by size:
3873 1012 859 836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6580
BstBI TT'CG_AA
Cuts at: 5384 5384
Size: 6580
BstEII G'GTnAC_C
Cuts at: 1184 3211 1184
Size: 2027 4553
BstYI r'GATC_y
Cuts at: 1972 2209 3065 3910 4277 4662 4679 6131 6217
Size: 237 856 845 367 385 17 1452 86
Cuts at: 6217 6229 6575 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 856 845 385 367 346 237
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3203 513
Size: 552 2138 3890
Fragments arranged by size:
3890 2138 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5841
Fragments arranged by size:
5841 683 56
BtsI GCAGTG_nn'
Cuts at: 2258 2437 3882 4096 4890 4910 2258
Size: 179 1445 214 794 20 3928
Fragments arranged by size:
3928 1445 794 214 179 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6545
DraI TTT'AAA
Cuts at: 3351 3884 3351
Size: 533 6047
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5587 809
Size: 507 4271 1802
Fragments arranged by size:
4271 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2683 3200 3815
Size: 54 1425 50 275 18 853 517 615
Cuts at: 3815 4027 4916 6545 8
Size: 212 889 1629 43
Fragments arranged by size:
1629 1425 889 853 615 517 275 212
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4027 1537
Size: 275 2215 4090
Fragments arranged by size:
4090 2215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2183 3627 3638 4510 1353
Size: 830 1444 11 872 3423
Fragments arranged by size:
3423 1444 872 830 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2207 5167 5541 5687 387
Size: 1064 138 618 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 618 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2501 2525 3584 3881 4643 5240
Size: 393 399 470 24 1059 297 762 597
Cuts at: 5240 6027 6295 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1059 787 762 597 470 399 393
297 268 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3513 3513
Size: 6580
EcoO109I rG'GnC_Cy
Cuts at: 3734 3734
Size: 6580
EcoRI G'AATT_C
Cuts at: 3889 4267 3889
Size: 378 6202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3061 3634 3061
Size: 573 6007
FspI TGC'GCA
Cuts at: 5086 5086
Size: 6580
HaeII r_GCGC'y
Cuts at: 1678 2133 2331 5727 1678
Size: 455 198 3396 2531
Fragments arranged by size:
3396 2531 455 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2160 2192 3084 3116 3246
Size: 32 135 32 588 32 892 32 130
Cuts at: 3246 3278 3362 3394 5221 5253 5295 5327 1373
Size: 32 84 32 1827 32 42 32 2626
Fragments arranged by size:
2626 1827 892 588 135 130 84 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3924 4170 669
Size: 988 281 1986 246 3079
Fragments arranged by size:
3079 1986 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3050 748
Size: 2302 4278
HpaI GTT'AAC
Cuts at: 4170 4170
Size: 6580
KpnI G_GTAC'C
Cuts at: 3905 3905
Size: 6580
MfeI C'AATT_G
Cuts at: 4179 4179
Size: 6580
MluI A'CGCG_T
Cuts at: 6456 6456
Size: 6580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3095 3151 5694 5878 1305
Size: 1790 56 2543 184 2007
Fragments arranged by size:
2543 2007 1790 184 56
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4758
Fragments arranged by size:
4758 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 2867 3249 3833 4538 4897 5056
Size: 648 1182 519 382 584 705 359 159
Cuts at: 5056 518
Size: 2042
Fragments arranged by size:
2042 1182 705 648 584 519 382 359
159
MspA1I CmG'CkG
Cuts at: 1606 2982 3996 4673 5821 6066 1606
Size: 1376 1014 677 1148 245 2120
Fragments arranged by size:
2120 1376 1148 1014 677 245
NaeI GCC'GGC
Cuts at: 3688 3688
Size: 6580
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6028
NdeI CA'TA_TG
Cuts at: 387 3500 387
Size: 3113 3467
NgoMIV G'CCGG_C
Cuts at: 3686 3686
Size: 6580
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6580
NotI GC'GGCC_GC
Cuts at: 4027 4027
Size: 6580
NspI r_CATG'y
Cuts at: 1132 1229 3938 5483 1132
Size: 97 2709 1545 2229
Fragments arranged by size:
2709 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6580
PciI A'CATG_T
Cuts at: 5479 5479
Size: 6580
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2220 1108
Size: 1112 5468
PmeI GTTT'AAAC
Cuts at: 3884 3884
Size: 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 6162 6194 4649
Size: 32 1481 32 5035
Fragments arranged by size:
5035 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6580
PsiI TTA'TAA
Cuts at: 4150 4150
Size: 6580
PstI C_TGCA'G
Cuts at: 830 2855 3932 830
Size: 2025 1077 3478
Fragments arranged by size:
3478 2025 1077
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6580
SalI G'TCGA_C
Cuts at: 1936 3922 1936
Size: 1986 4594
SbfI CC_TGCA'GG
Cuts at: 3932 3932
Size: 6580
ScaI AGT'ACT
Cuts at: 2138 4828 6540 2138
Size: 2690 1712 2178
Fragments arranged by size:
2690 2178 1712
SfcI C'TryA_G
Cuts at: 826 1045 2851 3337 3691 3928 5063 5744 5935
Size: 219 1806 486 354 237 1135 681 191
Cuts at: 5935 826
Size: 1471
Fragments arranged by size:
1806 1471 1135 681 486 354 237 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6580
SmaI CCC'GGG
Cuts at: 1872 3907 1872
Size: 2035 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2403 3870 4684 5585 5847 820
Size: 197 926 460 1467 814 901 262 1553
Fragments arranged by size:
1553 1467 926 901 814 460 262 197
SnaBI TAC'GTA
Cuts at: 493 4371 493
Size: 3878 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6580
SphI G_CATG'C
Cuts at: 1132 3938 1132
Size: 2806 3774
SspI AAT'ATT
Cuts at: 5 52 4504 5
Size: 47 4452 2081
Fragments arranged by size:
4452 2081 47
StuI AGG'CCT
Cuts at: 1517 2951 1517
Size: 1434 5146
StyI C'CwwG_G
Cuts at: 513 1065 4019 513
Size: 552 2954 3074
Fragments arranged by size:
3074 2954 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2178 2528 3247 3538 4607 4624 4777
Size: 1255 598 350 719 291 1069 17 153
Cuts at: 4777 4962 5407 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1069 719 598 445 350 291
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 2801 4826
Size: 274 80 33 51 1443 159 665 2025
Cuts at: 4826 6538 96
Size: 1712 138
Fragments arranged by size:
2025 1712 1443 665 274 159 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2686 2718 3125 3157 6540 6572 2686
Size: 32 407 32 3383 32 2694
Fragments arranged by size:
3383 2694 407 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2654 1316
Size: 1338 5242
XbaI T'CTAG_A
Cuts at: 3916 3916
Size: 6580
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6580
XmaI C'CCGG_G
Cuts at: 1870 3905 1870
Size: 2035 4545
XmnI GAAnn'nnTTC
Cuts at: 4709 4709
Size: 6580
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2434 4388 276
Size: 53 83 186 936 900 1954 2468
Fragments arranged by size:
2468 1954 936 900 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI