Restriction map for
pFN21AE5775
(Circular) (Six-base) MAPSORT of: pFN21AE5775.seq Check: 9580 from: 1 to: 6577
pFN21AE5775 6577 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2111 2111
Size: 6577
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4387 278
Size: 53 83 186 936 2851 2468
Fragments arranged by size:
2851 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3920 1937
Size: 1983 4594
Acc65I G'GTAC_C
Cuts at: 2822 3898 2822
Size: 1076 5501
AclI AA'CG_TT
Cuts at: 1563 4704 5077 1563
Size: 3141 373 3063
Fragments arranged by size:
3141 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2611 2810 3233 3269 3311 4640 6024 6292
Size: 979 199 423 36 42 1329 1384 268
Cuts at: 6292 1632
Size: 1917
Fragments arranged by size:
1917 1384 1329 979 423 268 199 42
36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6380
AflIII A'CryG_T
Cuts at: 1203 2484 5476 6453 1203
Size: 1281 2992 977 1327
Fragments arranged by size:
2992 1327 1281 977
AgeI A'CCGG_T
Cuts at: 5408 5408
Size: 6577
AhdI GACnn_n'nnGTC
Cuts at: 2857 5306 2857
Size: 2449 4128
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6577
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2463 1184
Size: 1279 5298
AlwNI CAG_nnn'CTG
Cuts at: 2164 3762 4320 5892 2164
Size: 1598 558 1572 2849
Fragments arranged by size:
2849 1598 1572 558
ApaLI G'TGCA_C
Cuts at: 2524 4634 5790 2524
Size: 2110 1156 3311
Fragments arranged by size:
3311 2110 1156
ApoI r'AATT_y
Cuts at: 1493 2041 2243 2253 2321 2899 3317 3553 3886
Size: 548 202 10 68 578 418 236 333
Cuts at: 3886 4112 4264 6373 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 578 548 418 333 236 226
202 152 68 10
AseI AT'TA_AT
Cuts at: 160 1032 5131 160
Size: 872 4099 1606
Fragments arranged by size:
4099 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6577
AvaI C'yCGr_G
Cuts at: 1870 1943 2130 3681 3873 3902 1870
Size: 73 187 1551 192 29 4545
Fragments arranged by size:
4545 1551 192 187 73 29
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2814 2847 2814
Size: 33 6544
BamHI G'GATC_C
Cuts at: 2134 3907 4274 2134
Size: 1773 367 4437
Fragments arranged by size:
4437 1773 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2016 2822 3898 5353 618
Size: 325 217 627 229 806 1076 1455 1842
Fragments arranged by size:
1842 1455 1076 806 627 325 229 217
BanII G_rGCy'C
Cuts at: 1391 2472 3296 3896 1391
Size: 1081 824 600 4072
Fragments arranged by size:
4072 1081 824 600
BbeI G_GCGC'C
Cuts at: 2020 2020
Size: 6577
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2946 3218 3368 928
Size: 971 1047 272 150 4137
Fragments arranged by size:
4137 1047 971 272 150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4768 4802 1903
Size: 34 2831 34 3678
Fragments arranged by size:
3678 2831 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2499 3609 4469 5679 2499
Size: 1110 860 1210 3397
Fragments arranged by size:
3397 1210 1110 860
BclI T'GATC_A
Cuts at: 1548 2248 1548
Size: 700 5877
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2126 5188 136
Size: 107 122 71 631 605 454 3062 1525
Fragments arranged by size:
3062 1525 631 605 454 122 107 71
BglII A'GATC_T
Cuts at: 3036 6572 3036
Size: 3536 3041
BlpI GC'TnA_GC
Cuts at: 3302 3994 3302
Size: 692 5885
Bme1580I G_kGCm'C
Cuts at: 1790 2528 4638 5794 1790
Size: 738 2110 1156 2573
Fragments arranged by size:
2573 2110 1156 738
BmgBI CAC'GTC
Cuts at: 1318 2485 1318
Size: 1167 5410
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2648 3826 4308
Size: 416 53 83 399 24 1231 1178 482
Cuts at: 4308 5266 442
Size: 958 1753
Fragments arranged by size:
1753 1231 1178 958 482 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 3535 5237 1239
Size: 854 1442 1702 2579
Fragments arranged by size:
2579 1702 1442 854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2960 4702 5567 5865 2960
Size: 1742 865 298 3672
Fragments arranged by size:
3672 1742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5240 882
Size: 617 3741 2219
Fragments arranged by size:
3741 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2371 4368 493
Size: 711 1167 1997 2702
Fragments arranged by size:
2702 1997 1167 711
BsaBI GATnn'nnATC
Cuts at: 6571 6571
Size: 6577
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2017 4384 4766 5401
Size: 53 83 186 936 484 2367 382 635
Cuts at: 5401 275
Size: 1451
Fragments arranged by size:
2367 1451 936 635 484 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5010 5408 5682 5829 6240 5010
Size: 398 274 147 411 5347
Fragments arranged by size:
5347 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3039 3069 1178
Size: 30 165 30 1636 30 4686
Fragments arranged by size:
4686 1636 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6577
BseYI C'CCAG_C
Cuts at: 2208 3804 5780 6276 2208
Size: 1596 1976 496 2509
Fragments arranged by size:
2509 1976 1596 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2188 2216 2358 3095 1163
Size: 573 452 28 142 737 4645
Fragments arranged by size:
4645 737 573 452 142 28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 4027 4788 5816 1540
Size: 275 186 120 1906 761 1028 2301
Fragments arranged by size:
2301 1906 1028 761 275 186 120
BsiHKAI G_wGCw'C
Cuts at: 2087 2528 3296 4638 4723 5794 2087
Size: 441 768 1342 85 1071 2870
Fragments arranged by size:
2870 1342 1071 768 441 85
BsmI GAATG_Cn'
Cuts at: 3255 4088 4181 3255
Size: 833 93 5651
Fragments arranged by size:
5651 833 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2472 2528 3296 3896 4638 4723
Size: 399 297 385 56 768 600 742 85
Cuts at: 4723 5794 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 768 742 600 399 385 297
85 56
BspEI T'CCGG_A
Cuts at: 6240 6240
Size: 6577
BspHI T'CATG_A
Cuts at: 3175 4466 6196 3175
Size: 1291 1730 3556
Fragments arranged by size:
3556 1730 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2111 3932 844
Size: 1267 1821 3489
Fragments arranged by size:
3489 1821 1267
BsrBI CCG'CTC
Cuts at: 1394 1823 2060 4464 1394
Size: 429 237 2404 3507
Fragments arranged by size:
3507 2404 429 237
BsrDI GCAATG_nn'
Cuts at: 65 1248 2751 3281 5072 5246 65
Size: 1183 1503 530 1791 174 1396
Fragments arranged by size:
1791 1503 1396 1183 530 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5221 5408 1700
Size: 110 3411 187 2869
Fragments arranged by size:
3411 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6577
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6577
BssSI C'ACGA_G
Cuts at: 2234 4637 5649 2234
Size: 2403 1012 3162
Fragments arranged by size:
3162 2403 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2164 794
Size: 1370 5207
BstBI TT'CG_AA
Cuts at: 5381 5381
Size: 6577
BstEII G'GTnAC_C
Cuts at: 1184 2642 1184
Size: 1458 5119
BstXI CCAn_nnnn'nTGG
Cuts at: 2145 2145
Size: 6577
BstYI r'GATC_y
Cuts at: 1972 2134 3036 3394 3907 4274 4659 4676 6128
Size: 162 902 358 513 367 385 17 1452
Cuts at: 6128 6214 6226 6572 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 902 513 385 367 358 346
162 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2451 513
Size: 552 939 447 4639
Fragments arranged by size:
4639 939 552 447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5838
Fragments arranged by size:
5838 683 56
BtsI GCAGTG_nn'
Cuts at: 2165 3233 3693 4093 4887 4907 2165
Size: 1068 460 400 794 20 3835
Fragments arranged by size:
3835 1068 794 460 400 20
ClaI AT'CG_AT
Cuts at: 1554 2181 1554
Size: 627 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6542
DraI TTT'AAA
Cuts at: 3583 3881 3583
Size: 298 6279
DraIII CAC_nnn'GTG
Cuts at: 3676 3676
Size: 6577
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5584 809
Size: 507 4268 1802
Fragments arranged by size:
4268 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 3025 4024
Size: 54 1425 50 275 18 288 907 999
Cuts at: 4024 4913 6542 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 999 907 889 288 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2118 4024 1537
Size: 275 306 1906 4090
Fragments arranged by size:
4090 1906 306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2983 4507 1353
Size: 1630 1524 3423
Fragments arranged by size:
3423 1630 1524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5164 5538 5684 387
Size: 1064 138 3575 374 146 1280
Fragments arranged by size:
3575 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3294 3294
Size: 6577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2611 2810 3233 3269 3311 3535
Size: 393 461 518 199 423 36 42 224
Cuts at: 3535 4640 5237 6024 6292 1239
Size: 1105 597 787 268 1524
Fragments arranged by size:
1524 1105 787 597 518 461 423 393
268 224 199 42 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2604 2604
Size: 6577
EcoO109I rG'GnC_Cy
Cuts at: 3796 3796
Size: 6577
EcoRI G'AATT_C
Cuts at: 3886 4264 3886
Size: 378 6199
FspI TGC'GCA
Cuts at: 5083 5083
Size: 6577
HaeII r_GCGC'y
Cuts at: 1678 2020 2116 5724 1678
Size: 342 96 3608 2531
Fragments arranged by size:
3608 2531 342 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2843 2875 3039 3071 3083
Size: 32 135 32 1271 32 164 32 12
Cuts at: 3083 3115 5218 5250 5292 5324 1373
Size: 32 2103 32 42 32 2626
Fragments arranged by size:
2626 2103 1271 164 135 42 32 32
32 32 32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3921 4167 669
Size: 988 281 1983 246 3079
Fragments arranged by size:
3079 1983 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3006 748
Size: 2258 4319
HpaI GTT'AAC
Cuts at: 4167 4167
Size: 6577
KasI G'GCGC_C
Cuts at: 2016 2016
Size: 6577
KpnI G_GTAC'C
Cuts at: 2826 3902 2826
Size: 1076 5501
MfeI C'AATT_G
Cuts at: 4176 4176
Size: 6577
MluI A'CGCG_T
Cuts at: 6453 6453
Size: 6577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5691 5875 1305
Size: 4386 184 2007
Fragments arranged by size:
4386 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3027 10
Size: 54 1425 343 1195 3560
Fragments arranged by size:
3560 1425 1195 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2716 3861 4535 4894 5053 518
Size: 648 1550 1145 674 359 159 2042
Fragments arranged by size:
2042 1550 1145 674 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2212 2499 2864 3993 4670 5818 6063 1606
Size: 606 287 365 1129 677 1148 245 2120
Fragments arranged by size:
2120 1148 1129 677 606 365 287 245
NarI GG'CG_CC
Cuts at: 2017 2017
Size: 6577
NcoI C'CATG_G
Cuts at: 513 1065 2004 2451 513
Size: 552 939 447 4639
Fragments arranged by size:
4639 939 552 447
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6577
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6577
NotI GC'GGCC_GC
Cuts at: 4024 4024
Size: 6577
NspI r_CATG'y
Cuts at: 1132 1229 3068 3935 5480 1132
Size: 97 1839 867 1545 2229
Fragments arranged by size:
2229 1839 1545 867 97
PasI CC'CwG_GG
Cuts at: 1348 2461 1348
Size: 1113 5464
PciI A'CATG_T
Cuts at: 5476 5476
Size: 6577
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6577
PfoI T'CCnGG_A
Cuts at: 2703 2983 2703
Size: 280 6297
PmeI GTTT'AAAC
Cuts at: 3881 3881
Size: 6577
PmlI CAC'GTG
Cuts at: 2371 2371
Size: 6577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4646 4678 6159 6191 4646
Size: 32 1481 32 5032
Fragments arranged by size:
5032 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6577
PsiI TTA'TAA
Cuts at: 4147 4147
Size: 6577
PstI C_TGCA'G
Cuts at: 830 2160 3929 830
Size: 1330 1769 3478
Fragments arranged by size:
3478 1769 1330
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6577
PvuII CAG'CTG
Cuts at: 2212 2499 2864 2212
Size: 287 365 5925
Fragments arranged by size:
5925 365 287
SacI G_AGCT'C
Cuts at: 3296 3296
Size: 6577
SalI G'TCGA_C
Cuts at: 1936 3919 1936
Size: 1983 4594
SbfI CC_TGCA'GG
Cuts at: 3929 3929
Size: 6577
ScaI AGT'ACT
Cuts at: 4825 6537 4825
Size: 1712 4865
SfcI C'TryA_G
Cuts at: 826 1045 2156 3766 3925 5060 5741 5932 826
Size: 219 1111 1610 159 1135 681 191 1471
Fragments arranged by size:
1610 1471 1135 1111 681 219 191 159
SfoI GGC'GCC
Cuts at: 2018 2018
Size: 6577
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6577
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6577
SmaI CCC'GGG
Cuts at: 1872 3904 1872
Size: 2032 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2975 4681 5582 5844 820
Size: 197 926 1032 1706 901 262 1553
Fragments arranged by size:
1706 1553 1032 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4368 493
Size: 3875 2702
SpeI A'CTAG_T
Cuts at: 152 3428 152
Size: 3276 3301
SphI G_CATG'C
Cuts at: 1132 3935 1132
Size: 2803 3774
SspI AAT'ATT
Cuts at: 5 52 4501 5
Size: 47 4449 2081
Fragments arranged by size:
4449 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6577
StyI C'CwwG_G
Cuts at: 513 1065 2004 2451 3193 4016 513
Size: 552 939 447 742 823 3074
Fragments arranged by size:
3074 939 823 742 552 447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2222 3818 4604 4621 4774 4959 5404
Size: 1255 642 1596 786 17 153 185 445
Cuts at: 5404 325
Size: 1498
Fragments arranged by size:
1596 1498 1255 786 642 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3386 4823 6535
Size: 274 80 33 51 1443 1409 1437 1712
Cuts at: 6535 96
Size: 138
Fragments arranged by size:
1712 1443 1437 1409 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6537 6569 6537
Size: 32 6545
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6577
XbaI T'CTAG_A
Cuts at: 3259 3913 3259
Size: 654 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2458 2458
Size: 6577
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6577
XmaI C'CCGG_G
Cuts at: 1870 3902 1870
Size: 2032 4545
XmnI GAAnn'nnTTC
Cuts at: 2943 4706 2943
Size: 1763 4814
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4385 276
Size: 53 83 186 936 2851 2468
Fragments arranged by size:
2851 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI