Restriction map for
pFN21AE5769
(Circular) (Six-base) MAPSORT of: pFN21AE5769.seq Check: 6943 from: 1 to: 6466
pFN21AE5769 6466 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3346 3346
Size: 6466
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4276 278
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3809 1937
Size: 1872 4594
Acc65I G'GTAC_C
Cuts at: 3787 3787
Size: 6466
AclI AA'CG_TT
Cuts at: 1563 4593 4966 1563
Size: 3030 373 3063
Fragments arranged by size:
3063 3030 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2961 3598 4529 5913 6181 1632
Size: 1329 637 931 1384 268 1917
Fragments arranged by size:
1917 1384 1329 931 637 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6269
AflIII A'CryG_T
Cuts at: 1203 2955 5365 6342 1203
Size: 1752 2410 977 1327
Fragments arranged by size:
2410 1752 1327 977
AgeI A'CCGG_T
Cuts at: 5297 5297
Size: 6466
AhdI GACnn_n'nnGTC
Cuts at: 5195 5195
Size: 6466
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6466
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6466
AlwNI CAG_nnn'CTG
Cuts at: 2389 2734 2782 4209 5781 2389
Size: 345 48 1427 1572 3074
Fragments arranged by size:
3074 1572 1427 345 48
ApaI G_GGCC'C
Cuts at: 2410 2410
Size: 6466
ApaLI G'TGCA_C
Cuts at: 2379 4523 5679 2379
Size: 2144 1156 3166
Fragments arranged by size:
3166 2144 1156
ApoI r'AATT_y
Cuts at: 1493 3775 4001 4153 6262 1493
Size: 2282 226 152 2109 1697
Fragments arranged by size:
2282 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5020 160
Size: 872 3988 1606
Fragments arranged by size:
3988 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2331 2359 2426 2798 3051 3312
Size: 73 67 321 28 67 372 253 261
Cuts at: 3312 3791 1870
Size: 479 4545
Fragments arranged by size:
4545 479 372 321 261 253 73 67
67 28
BamHI G'GATC_C
Cuts at: 3796 4163 3796
Size: 367 6099
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2341 2422 2821 2870 3323
Size: 325 217 627 554 81 399 49 453
Cuts at: 3323 3421 3787 5242 618
Size: 98 366 1455 1842
Fragments arranged by size:
1842 1455 627 554 453 399 366 325
217 98 81 49
BanII G_rGCy'C
Cuts at: 1391 2201 2410 2436 3288 3370 3489 3609 3785
Size: 810 209 26 852 82 119 120 176
Cuts at: 3785 1391
Size: 4072
Fragments arranged by size:
4072 852 810 209 176 120 119 82
26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2698 928
Size: 971 799 4696
Fragments arranged by size:
4696 971 799
BbvCI CC'TCA_GC
Cuts at: 2543 2543
Size: 6466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2224 2258 4657 4691 1903
Size: 34 287 34 2399 34 3678
Fragments arranged by size:
3678 2399 287 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4358 5568 4358
Size: 1210 5256
BclI T'GATC_A
Cuts at: 1548 3435 1548
Size: 1887 4579
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2466 5077 136
Size: 107 122 71 631 605 794 2611 1525
Fragments arranged by size:
2611 1525 794 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6461 6461
Size: 6466
BlpI GC'TnA_GC
Cuts at: 3122 3883 3122
Size: 761 5705
Bme1580I G_kGCm'C
Cuts at: 1790 2383 2402 2410 2427 2826 3426 4527 5683
Size: 593 19 8 17 399 600 1101 1156
Cuts at: 5683 1790
Size: 2573
Fragments arranged by size:
2573 1156 1101 600 593 399 19 17
8
BmgBI CAC'GTC
Cuts at: 1318 3168 1318
Size: 1850 4616
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2404 2405 3383
Size: 416 53 83 399 24 987 1 978
Cuts at: 3383 4197 5155 442
Size: 814 958 1753
Fragments arranged by size:
1753 987 978 958 814 416 399 83
53 24 1
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2201 2201
Size: 6466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2696 2837 3023 5126 1239
Size: 932 525 141 186 2103 2579
Fragments arranged by size:
2579 2103 932 525 186 141
Bpu10I CC'TnA_GC
Cuts at: 2543 2543
Size: 6466
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2966 4591 5456 5754 2966
Size: 1625 865 298 3678
Fragments arranged by size:
3678 1625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2672 3062 5129 882
Size: 617 1173 390 2067 2219
Fragments arranged by size:
2219 2067 1173 617 390
BsaAI yAC'GTr
Cuts at: 493 1204 4257 493
Size: 711 3053 2702
Fragments arranged by size:
3053 2702 711
BsaBI GATnn'nnATC
Cuts at: 6460 6460
Size: 6466
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2588 4273 4655 5290
Size: 53 83 186 936 1055 1685 382 635
Cuts at: 5290 275
Size: 1451
Fragments arranged by size:
1685 1451 1055 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4899 5297 5571 5718 6129 4899
Size: 398 274 147 411 5236
Fragments arranged by size:
5236 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3231 3261 3342 3372 1178
Size: 30 165 30 1828 30 81 30 4272
Fragments arranged by size:
4272 1828 165 81 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6466
BseYI C'CCAG_C
Cuts at: 2044 2722 3010 3061 3078 3184 5669 6165 2044
Size: 678 288 51 17 106 2485 496 2345
Fragments arranged by size:
2485 2345 678 496 288 106 51 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 2540 2643 3647 1163
Size: 573 482 322 103 1004 3982
Fragments arranged by size:
3982 1004 573 482 322 103
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3916 4677 5705 1540
Size: 275 186 1915 761 1028 2301
Fragments arranged by size:
2301 1915 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2383 2436 3288 3370 3489 4527 4612 5683 2383
Size: 53 852 82 119 1038 85 1071 3166
Fragments arranged by size:
3166 1071 1038 852 119 85 82 53
BsmI GAATG_Cn'
Cuts at: 3049 3977 4070 3049
Size: 928 93 5445
Fragments arranged by size:
5445 928 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2330 2969 3733 2330
Size: 639 764 5063
Fragments arranged by size:
5063 764 639
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2383 2402 2410 2427 2436 2826
Size: 399 411 182 19 8 17 9 390
Cuts at: 2826 3288 3370 3426 3489 3609 3785 4527 4612
Size: 462 82 56 63 120 176 742 85
Cuts at: 4612 5683 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 462 411 399 390 182
176 120 85 82 63 56 19 17
9 8
BspEI T'CCGG_A
Cuts at: 6129 6129
Size: 6466
BspHI T'CATG_A
Cuts at: 3516 4355 6085 3516
Size: 839 1730 3897
Fragments arranged by size:
3897 1730 839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3066 3346 3821 844
Size: 2222 280 475 3489
Fragments arranged by size:
3489 2222 475 280
BsrBI CCG'CTC
Cuts at: 1394 1823 3551 4353 1394
Size: 429 1728 802 3507
Fragments arranged by size:
3507 1728 802 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2745 3555 4961 5135 65
Size: 1183 1497 810 1406 174 1396
Fragments arranged by size:
1497 1406 1396 1183 810 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2344 5110 5297 1700
Size: 110 534 2766 187 2869
Fragments arranged by size:
2869 2766 534 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6466
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6466
BssSI C'ACGA_G
Cuts at: 4526 5538 4526
Size: 1012 5454
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2870 794
Size: 2076 4390
BstBI TT'CG_AA
Cuts at: 5270 5270
Size: 6466
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6466
BstXI CCAn_nnnn'nTGG
Cuts at: 2652 2674 3647 2652
Size: 22 973 5471
Fragments arranged by size:
5471 973 22
BstYI r'GATC_y
Cuts at: 1972 3796 4163 4548 4565 6017 6103 6115 6461
Size: 1824 367 385 17 1452 86 12 346
Cuts at: 6461 1972
Size: 1977
Fragments arranged by size:
1977 1824 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2630 2637 3737 2630
Size: 7 1100 5359
Fragments arranged by size:
5359 1100 7
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 2205 2437 2464 2651 2682
Size: 552 939 21 180 232 27 187 31
Cuts at: 2682 3154 3204 3257 3417 3646 513
Size: 472 50 53 160 229 3333
Fragments arranged by size:
3333 939 552 472 232 229 187 180
160 53 50 31 27 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2203 2488 3257 3319 487
Size: 683 56 977 285 769 62 3634
Fragments arranged by size:
3634 977 769 683 285 62 56
BtsI GCAGTG_nn'
Cuts at: 3348 3982 4776 4796 3348
Size: 634 794 20 5018
Fragments arranged by size:
5018 794 634 20
ClaI AT'CG_AT
Cuts at: 1554 3099 1554
Size: 1545 4921
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6431
DraI TTT'AAA
Cuts at: 3770 3770
Size: 6466
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2156 3166 5473 809
Size: 507 840 1010 2307 1802
Fragments arranged by size:
2307 1802 1010 840 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2346 2467 2664
Size: 54 1425 50 275 18 516 121 197
Cuts at: 2664 2685 2784 2931 3103 3138 3552 3649 3913
Size: 21 99 147 172 35 414 97 264
Cuts at: 3913 4802 6431 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 516 414 275 264 197
172 147 121 99 97 54 50 43
35 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3913 1537
Size: 275 2101 4090
Fragments arranged by size:
4090 2101 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2612 3737 4396 1353
Size: 1259 1125 659 3423
Fragments arranged by size:
3423 1259 1125 659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2286 3077 5053 5427 5573 387
Size: 1064 138 697 791 1976 374 146 1280
Fragments arranged by size:
1976 1280 1064 791 697 374 146 138
EcoICRI GAG'CTC
Cuts at: 2434 3286 3368 3487 2434
Size: 852 82 119 5413
Fragments arranged by size:
5413 852 119 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2696 2837 2961 3023 3598 4529
Size: 393 539 525 141 124 62 575 931
Cuts at: 4529 5126 5913 6181 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 931 787 597 575 539 525 393
268 141 124 62
EcoO109I rG'GnC_Cy
Cuts at: 2104 2406 2641 2779 3016 3055 3669 3711 2104
Size: 302 235 138 237 39 614 42 4859
Fragments arranged by size:
4859 614 302 237 235 138 42 39
EcoRI G'AATT_C
Cuts at: 3775 4153 3775
Size: 378 6088
FspI TGC'GCA
Cuts at: 4972 4972
Size: 6466
HaeII r_GCGC'y
Cuts at: 1678 2063 3204 5613 1678
Size: 385 1141 2409 2531
Fragments arranged by size:
2531 2409 1141 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2166 2198 2265 2297 5107
Size: 32 135 32 594 32 67 32 2810
Cuts at: 5107 5139 5181 5213 1373
Size: 32 42 32 2626
Fragments arranged by size:
2810 2626 594 135 67 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2248 2592 3580 3810 4056 669
Size: 988 281 310 344 988 230 246 3079
Fragments arranged by size:
3079 988 988 344 310 281 246 230
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6466
HpaI GTT'AAC
Cuts at: 4056 4056
Size: 6466
KpnI G_GTAC'C
Cuts at: 3791 3791
Size: 6466
MfeI C'AATT_G
Cuts at: 4065 4065
Size: 6466
MluI A'CGCG_T
Cuts at: 6342 6342
Size: 6466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5580 5764 1305
Size: 4275 184 2007
Fragments arranged by size:
4275 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2666 2687 2786 2933 3651
Size: 54 1425 343 834 21 99 147 718
Cuts at: 3651 10
Size: 2825
Fragments arranged by size:
2825 1425 834 718 343 147 99 54
21
MslI CAynn'nnrTG
Cuts at: 518 1166 2650 4424 4783 4942 518
Size: 648 1484 1774 359 159 2042
Fragments arranged by size:
2042 1774 1484 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2488 2767 3882 4559 5707 5952 1606
Size: 882 279 1115 677 1148 245 2120
Fragments arranged by size:
2120 1148 1115 882 677 279 245
NaeI GCC'GGC
Cuts at: 2346 2346
Size: 6466
NcoI C'CATG_G
Cuts at: 513 1065 2004 2464 2651 3417 513
Size: 552 939 460 187 766 3562
Fragments arranged by size:
3562 939 766 552 460 187
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6466
NgoMIV G'CCGG_C
Cuts at: 2344 2344
Size: 6466
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6466
NotI GC'GGCC_GC
Cuts at: 3913 3913
Size: 6466
NspI r_CATG'y
Cuts at: 1132 1229 2528 3578 3824 5369 1132
Size: 97 1299 1050 246 1545 2229
Fragments arranged by size:
2229 1545 1299 1050 246 97
PasI CC'CwG_GG
Cuts at: 1348 2478 2827 3448 1348
Size: 1130 349 621 4366
Fragments arranged by size:
4366 1130 621 349
PciI A'CATG_T
Cuts at: 5365 5365
Size: 6466
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2734 1108
Size: 1626 4840
PfoI T'CCnGG_A
Cuts at: 3288 3370 3288
Size: 82 6384
PmeI GTTT'AAAC
Cuts at: 3770 3770
Size: 6466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4535 4567 6048 6080 4535
Size: 32 1481 32 4921
Fragments arranged by size:
4921 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2641 2779 3016 3055 2641
Size: 138 237 39 6052
Fragments arranged by size:
6052 237 138 39
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6466
PsiI TTA'TAA
Cuts at: 4036 4036
Size: 6466
PspOMI G'GGCC_C
Cuts at: 2406 2406
Size: 6466
PstI C_TGCA'G
Cuts at: 830 2778 2866 3343 3818 830
Size: 1948 88 477 475 3478
Fragments arranged by size:
3478 1948 477 475 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6466
PvuII CAG'CTG
Cuts at: 2488 2767 2488
Size: 279 6187
SacI G_AGCT'C
Cuts at: 2436 3288 3370 3489 2436
Size: 852 82 119 5413
Fragments arranged by size:
5413 852 119 82
SalI G'TCGA_C
Cuts at: 1936 3808 1936
Size: 1872 4594
SanDI GG'GwC_CC
Cuts at: 3016 3016
Size: 6466
SapI GCTCTTCn'nnn_
Cuts at: 2612 2612
Size: 6466
SbfI CC_TGCA'GG
Cuts at: 2866 3818 2866
Size: 952 5514
ScaI AGT'ACT
Cuts at: 4714 6426 4714
Size: 1712 4754
SfcI C'TryA_G
Cuts at: 826 1045 2774 2862 3130 3339 3814 4949 5630
Size: 219 1729 88 268 209 475 1135 681
Cuts at: 5630 5821 826
Size: 191 1471
Fragments arranged by size:
1729 1471 1135 681 475 268 219 209
191 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6466
SmaI CCC'GGG
Cuts at: 1872 2012 2333 2428 3053 3314 3793 1872
Size: 140 321 95 625 261 479 4545
Fragments arranged by size:
4545 625 479 321 261 140 95
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2981 4570 5471 5733 820
Size: 197 926 1038 1589 901 262 1553
Fragments arranged by size:
1589 1553 1038 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4257 493
Size: 3764 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6466
SphI G_CATG'C
Cuts at: 1132 3824 1132
Size: 2692 3774
SspI AAT'ATT
Cuts at: 5 52 4390 5
Size: 47 4338 2081
Fragments arranged by size:
4338 2081 47
StuI AGG'CCT
Cuts at: 1517 2123 2635 2938 3146 1517
Size: 606 512 303 208 4837
Fragments arranged by size:
4837 606 512 303 208
StyI C'CwwG_G
Cuts at: 513 1065 2004 2154 2464 2651 2751 2991 3417
Size: 552 939 150 310 187 100 240 426
Cuts at: 3417 3905 513
Size: 488 3074
Fragments arranged by size:
3074 939 552 488 426 310 240 187
150 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3350 4493 4510 4663 4848 5293 325
Size: 1255 1770 1143 17 153 185 445 1498
Fragments arranged by size:
1770 1498 1255 1143 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2844 4712 6424
Size: 274 80 33 51 1443 867 1868 1712
Cuts at: 6424 96
Size: 138
Fragments arranged by size:
1868 1712 1443 867 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3231 3263 3342 3374 6426 6458 3231
Size: 32 79 32 3052 32 3239
Fragments arranged by size:
3239 3052 79 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6466
XbaI T'CTAG_A
Cuts at: 3802 3802
Size: 6466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2108 2224 2462 2695 3098 2108
Size: 116 238 233 403 5476
Fragments arranged by size:
5476 403 238 233 116
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6466
XmaI C'CCGG_G
Cuts at: 1870 2010 2331 2426 3051 3312 3791 1870
Size: 140 321 95 625 261 479 4545
Fragments arranged by size:
4545 625 479 321 261 140 95
XmnI GAAnn'nnTTC
Cuts at: 4595 4595
Size: 6466
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4274 276
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SexAI SfiI SfoI SrfI SwaI