Restriction map for
pFN21AE5767
(Circular) (Six-base) MAPSORT of: pFN21AE5767.seq Check: 7999 from: 1 to: 6502
pFN21AE5767 6502 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2013 4312 278
Size: 53 83 186 936 477 2299 2468
Fragments arranged by size:
2468 2299 936 477 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2955 3845 1937
Size: 1018 890 4594
Fragments arranged by size:
4594 1018 890
Acc65I G'GTAC_C
Cuts at: 3823 3823
Size: 6502
AclI AA'CG_TT
Cuts at: 1563 4629 5002 1563
Size: 3066 373 3063
Fragments arranged by size:
3066 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2117 2271 2519 2692 4565 5949 6217 1632
Size: 485 154 248 173 1873 1384 268 1917
Fragments arranged by size:
1917 1873 1384 485 268 248 173 154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6305
AflIII A'CryG_T
Cuts at: 1203 5401 6378 1203
Size: 4198 977 1327
Fragments arranged by size:
4198 1327 977
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 5231 5231
Size: 6502
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6502
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2455 2487 2455
Size: 32 6470
AlwNI CAG_nnn'CTG
Cuts at: 2140 4245 5817 2140
Size: 2105 1572 2825
Fragments arranged by size:
2825 2105 1572
ApaLI G'TGCA_C
Cuts at: 4559 5715 4559
Size: 1156 5346
ApoI r'AATT_y
Cuts at: 1493 2048 2448 2772 2834 3142 3811 4037 4189
Size: 555 400 324 62 308 669 226 152
Cuts at: 4189 6298 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 669 555 400 324 308 226
152 62
AseI AT'TA_AT
Cuts at: 160 1032 3315 5056 160
Size: 872 2283 1741 1606
Fragments arranged by size:
2283 1741 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 3827 1870
Size: 73 1884 4545
Fragments arranged by size:
4545 1884 73
BamHI G'GATC_C
Cuts at: 3832 4199 3832
Size: 367 6135
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3823 5278 618
Size: 325 217 627 2036 1455 1842
Fragments arranged by size:
2036 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2716 3300 3821 1391
Size: 1325 584 521 4072
Fragments arranged by size:
4072 1325 584 521
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 2361 3798 928
Size: 971 383 79 1437 3632
Fragments arranged by size:
3632 1437 971 383 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4693 4727 1903
Size: 34 2756 34 3678
Fragments arranged by size:
3678 2756 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2790 4394 5604 2790
Size: 1604 1210 3688
Fragments arranged by size:
3688 1604 1210
BclI T'GATC_A
Cuts at: 1548 3456 1548
Size: 1908 4594
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2034 5113 136
Size: 107 122 71 631 605 362 3079 1525
Fragments arranged by size:
3079 1525 631 605 362 122 107 71
BglII A'GATC_T
Cuts at: 6497 6497
Size: 6502
BlpI GC'TnA_GC
Cuts at: 3919 3919
Size: 6502
Bme1580I G_kGCm'C
Cuts at: 1790 4563 5719 1790
Size: 2773 1156 2573
Fragments arranged by size:
2773 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2455 1318
Size: 1137 5365
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2588 3000 4233
Size: 416 53 83 399 24 1171 412 1233
Cuts at: 4233 5191 442
Size: 958 1753
Fragments arranged by size:
1753 1233 1171 958 416 412 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3422 1056
Size: 2366 4136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5162 1239
Size: 3923 2579
Bpu10I CC'TnA_GC
Cuts at: 3735 3735
Size: 6502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4627 5492 5790 4627
Size: 865 298 5339
Fragments arranged by size:
5339 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2994 3185 5165 882
Size: 617 1495 191 1980 2219
Fragments arranged by size:
2219 1980 1495 617 191
BsaAI yAC'GTr
Cuts at: 493 1204 4293 493
Size: 711 3089 2702
Fragments arranged by size:
3089 2702 711
BsaBI GATnn'nnATC
Cuts at: 6496 6496
Size: 6502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 4309 4691 5326
Size: 53 83 186 936 477 2299 382 635
Cuts at: 5326 275
Size: 1451
Fragments arranged by size:
2299 1451 936 635 477 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2390 3194 4935 5333 5607 5754 6165 2390
Size: 804 1741 398 274 147 411 2727
Fragments arranged by size:
2727 1741 804 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6277
Fragments arranged by size:
6277 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6502
BseYI C'CCAG_C
Cuts at: 3605 5705 6201 3605
Size: 2100 496 3906
Fragments arranged by size:
3906 2100 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3013 1163
Size: 573 1277 4652
Fragments arranged by size:
4652 1277 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3952 4713 5741 1540
Size: 275 186 1951 761 1028 2301
Fragments arranged by size:
2301 1951 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3300 4563 4648 5719 3300
Size: 1263 85 1071 4083
Fragments arranged by size:
4083 1263 1071 85
BsmI GAATG_Cn'
Cuts at: 2664 4013 4106 2664
Size: 1349 93 5060
Fragments arranged by size:
5060 1349 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2053 2053
Size: 6502
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2716 3300 3821 4563 4648 5719 1391
Size: 399 926 584 521 742 85 1071 2174
Fragments arranged by size:
2174 1071 926 742 584 521 399 85
BspEI T'CCGG_A
Cuts at: 2390 6165 2390
Size: 3775 2727
BspHI T'CATG_A
Cuts at: 3292 4391 6121 3292
Size: 1099 1730 3673
Fragments arranged by size:
3673 1730 1099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2543 3857 844
Size: 1699 1314 3489
Fragments arranged by size:
3489 1699 1314
BsrBI CCG'CTC
Cuts at: 1394 1823 4389 1394
Size: 429 2566 3507
Fragments arranged by size:
3507 2566 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4997 5171 65
Size: 1183 3749 174 1396
Fragments arranged by size:
3749 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2028 5146 5333 1700
Size: 110 218 3118 187 2869
Fragments arranged by size:
3118 2869 218 187 110
BsrGI T'GTAC_A
Cuts at: 96 3407 96
Size: 3311 3191
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6502
BssSI C'ACGA_G
Cuts at: 2056 2733 4562 5574 2056
Size: 677 1829 1012 2984
Fragments arranged by size:
2984 1829 1012 677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3071 794
Size: 2277 4225
BstBI TT'CG_AA
Cuts at: 5306 5306
Size: 6502
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6502
BstXI CCAn_nnnn'nTGG
Cuts at: 3022 3022
Size: 6502
BstYI r'GATC_y
Cuts at: 1972 2639 2911 3185 3832 4199 4584 4601 6053
Size: 667 272 274 647 367 385 17 1452
Cuts at: 6053 6139 6151 6497 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 667 647 385 367 346 274
272 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5011
Fragments arranged by size:
5011 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5763
Fragments arranged by size:
5763 683 56
BtsI GCAGTG_nn'
Cuts at: 2826 4018 4812 4832 2826
Size: 1192 794 20 4496
Fragments arranged by size:
4496 1192 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6467
DraI TTT'AAA
Cuts at: 3439 3806 3439
Size: 367 6135
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5509 809
Size: 507 4193 1802
Fragments arranged by size:
4193 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3207 3949 4838
Size: 54 1425 50 275 18 1377 742 889
Cuts at: 4838 6467 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1377 889 742 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3949 1537
Size: 275 2137 4090
Fragments arranged by size:
4090 2137 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2223 2243 3043 3511 4432 1353
Size: 870 20 800 468 921 3423
Fragments arranged by size:
3423 921 870 800 468 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5089 5463 5609 387
Size: 1064 138 3500 374 146 1280
Fragments arranged by size:
3500 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3298 3298
Size: 6502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2271 2519 2692 4565 5162 5949
Size: 393 485 154 248 173 1873 597 787
Cuts at: 5949 6217 1239
Size: 268 1524
Fragments arranged by size:
1873 1524 787 597 485 393 268 248
173 154
EcoRI G'AATT_C
Cuts at: 3142 3811 4189 3142
Size: 669 378 5455
Fragments arranged by size:
5455 669 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2443 3803 2443
Size: 1360 5142
FspI TGC'GCA
Cuts at: 5008 5008
Size: 6502
HaeII r_GCGC'y
Cuts at: 1678 5649 1678
Size: 3971 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5143 5175 5217 5249 1373
Size: 32 135 32 3571 32 42 32 2626
Fragments arranged by size:
3571 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3846 4092 669
Size: 988 281 1908 246 3079
Fragments arranged by size:
3079 1908 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6502
HpaI GTT'AAC
Cuts at: 4092 4092
Size: 6502
KpnI G_GTAC'C
Cuts at: 3827 3827
Size: 6502
MfeI C'AATT_G
Cuts at: 2481 4101 2481
Size: 1620 4882
MluI A'CGCG_T
Cuts at: 6378 6378
Size: 6502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 2657 3163 3497 5616 5800 1305
Size: 1074 278 506 334 2119 184 2007
Fragments arranged by size:
2119 2007 1074 506 334 278 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4680
Fragments arranged by size:
4680 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3122 4460 4819 4978 518
Size: 648 1956 1338 359 159 2042
Fragments arranged by size:
2042 1956 1338 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2683 3007 3918 4595 5743 5988 1606
Size: 1077 324 911 677 1148 245 2120
Fragments arranged by size:
2120 1148 1077 911 677 324 245
NaeI GCC'GGC
Cuts at: 2030 2030
Size: 6502
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5011
Fragments arranged by size:
5011 939 552
NdeI CA'TA_TG
Cuts at: 387 3076 387
Size: 2689 3813
NgoMIV G'CCGG_C
Cuts at: 2028 2028
Size: 6502
NheI G'CTAG_C
Cuts at: 1052 3418 1052
Size: 2366 4136
NotI GC'GGCC_GC
Cuts at: 3949 3949
Size: 6502
NspI r_CATG'y
Cuts at: 1132 1229 3860 5405 1132
Size: 97 2631 1545 2229
Fragments arranged by size:
2631 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6502
PciI A'CATG_T
Cuts at: 5401 5401
Size: 6502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6502
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4571 4603 6084 6116 4571
Size: 32 1481 32 4957
Fragments arranged by size:
4957 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 4072 4072
Size: 6502
PstI C_TGCA'G
Cuts at: 830 3151 3854 830
Size: 2321 703 3478
Fragments arranged by size:
3478 2321 703
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
PvuII CAG'CTG
Cuts at: 2683 3007 2683
Size: 324 6178
SacI G_AGCT'C
Cuts at: 3300 3300
Size: 6502
SalI G'TCGA_C
Cuts at: 1936 3844 1936
Size: 1908 4594
SapI GCTCTTCn'nnn_
Cuts at: 3043 3511 3043
Size: 468 6034
SbfI CC_TGCA'GG
Cuts at: 3854 3854
Size: 6502
ScaI AGT'ACT
Cuts at: 4750 6462 4750
Size: 1712 4790
SfcI C'TryA_G
Cuts at: 826 1045 2337 3147 3159 3850 4985 5666 5857
Size: 219 1292 810 12 691 1135 681 191
Cuts at: 5857 826
Size: 1471
Fragments arranged by size:
1471 1292 1135 810 691 681 219 191
12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6502
SmaI CCC'GGG
Cuts at: 1872 3829 1872
Size: 1957 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4606 5507 5769 820
Size: 197 926 2663 901 262 1553
Fragments arranged by size:
2663 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6502
SphI G_CATG'C
Cuts at: 1132 3860 1132
Size: 2728 3774
SspI AAT'ATT
Cuts at: 5 52 4426 5
Size: 47 4374 2081
Fragments arranged by size:
4374 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6502
StyI C'CwwG_G
Cuts at: 513 1065 2004 2458 3941 513
Size: 552 939 454 1483 3074
Fragments arranged by size:
3074 1483 939 552 454
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3597 4529 4546 4699 4884 5329 325
Size: 1255 2017 932 17 153 185 445 1498
Fragments arranged by size:
2017 1498 1255 932 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3407 3704 4748
Size: 274 80 33 51 1443 1430 297 1044
Cuts at: 4748 6460 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1430 1044 297 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6462 6494 6462
Size: 32 6470
Tth111I GACn'n_nGTC
Cuts at: 1316 3113 1316
Size: 1797 4705
XbaI T'CTAG_A
Cuts at: 2545 3838 2545
Size: 1293 5209
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6502
XmaI C'CCGG_G
Cuts at: 1870 3827 1870
Size: 1957 4545
XmnI GAAnn'nnTTC
Cuts at: 3288 4631 3288
Size: 1343 5159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2011 4310 276
Size: 53 83 186 936 477 2299 2468
Fragments arranged by size:
2468 2299 936 477 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI