Restriction map for
pFN21AE5766
(Circular) (Six-base) MAPSORT of: pFN21AE5766.seq Check: 8212 from: 1 to: 6472
pFN21AE5766 6472 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2205 4282 278
Size: 53 83 186 936 669 2077 2468
Fragments arranged by size:
2468 2077 936 669 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2579 3815 1937
Size: 642 1236 4594
Fragments arranged by size:
4594 1236 642
Acc65I G'GTAC_C
Cuts at: 2483 3793 2483
Size: 1310 5162
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2414 2523 2886 3255 3401 4535 5919
Size: 608 174 109 363 369 146 1134 1384
Cuts at: 5919 6187 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1134 608 369 363 268 174
146 109
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 5371 6348 1203
Size: 4168 977 1327
Fragments arranged by size:
4168 1327 977
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 2258 2503 5201 2258
Size: 245 2698 3529
Fragments arranged by size:
3529 2698 245
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6472
AlwNI CAG_nnn'CTG
Cuts at: 3224 4215 5787 3224
Size: 991 1572 3909
Fragments arranged by size:
3909 1572 991
ApaI G_GGCC'C
Cuts at: 2513 2513
Size: 6472
ApaLI G'TGCA_C
Cuts at: 4529 5685 4529
Size: 1156 5316
ApoI r'AATT_y
Cuts at: 1493 2072 2093 2549 3341 3716 3781 4007 4159
Size: 579 21 456 792 375 65 226 152
Cuts at: 4159 6268 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 792 579 456 375 226 152
65 21
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 3638 3797 1870
Size: 73 1695 159 4545
Fragments arranged by size:
4545 1695 159 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2645 2678 2645
Size: 33 6439
BamHI G'GATC_C
Cuts at: 3802 4169 3802
Size: 367 6105
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2483 3793 5248 618
Size: 325 217 627 696 1310 1455 1842
Fragments arranged by size:
1842 1455 1310 696 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2513 3791 1391
Size: 1122 1278 4072
Fragments arranged by size:
4072 1278 1122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2254 3389 3594 928
Size: 971 355 1135 205 3806
Fragments arranged by size:
3806 1135 971 355 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2671 2705 4663 4697 1903
Size: 34 50 34 650 34 1958 34 3678
Fragments arranged by size:
3678 1958 650 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2673 4364 5574 2673
Size: 1691 1210 3571
Fragments arranged by size:
3571 1691 1210
BclI T'GATC_A
Cuts at: 1548 3606 1548
Size: 2058 4414
BfrBI ATG'CAT
Cuts at: 3086 3086
Size: 6472
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5083 136
Size: 107 122 71 631 605 3411 1525
Fragments arranged by size:
3411 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6467 6467
Size: 6472
BlpI GC'TnA_GC
Cuts at: 3889 3889
Size: 6472
Bme1580I G_kGCm'C
Cuts at: 1790 2513 4533 5689 1790
Size: 723 2020 1156 2573
Fragments arranged by size:
2573 2020 1156 723
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6472
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4203 5161 442
Size: 416 53 83 399 24 2786 958 1753
Fragments arranged by size:
2786 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2268 2958 5132 1239
Size: 884 145 690 2174 2579
Fragments arranged by size:
2579 2174 884 690 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4597 5462 5760 4597
Size: 865 298 5309
Fragments arranged by size:
5309 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5135 882
Size: 617 3636 2219
Fragments arranged by size:
3636 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4263 493
Size: 711 3059 2702
Fragments arranged by size:
3059 2702 711
BsaBI GATnn'nnATC
Cuts at: 3036 3053 6466 3036
Size: 17 3413 3042
Fragments arranged by size:
3413 3042 17
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2202 4279 4661 5296
Size: 53 83 186 936 669 2077 382 635
Cuts at: 5296 275
Size: 1451
Fragments arranged by size:
2077 1451 936 669 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4905 5303 5577 5724 6135 4905
Size: 398 274 147 411 5242
Fragments arranged by size:
5242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6247
Fragments arranged by size:
6247 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3730 1186
Size: 2544 3928
BseYI C'CCAG_C
Cuts at: 5675 6171 5675
Size: 496 5976
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3669 1163
Size: 573 1933 3966
Fragments arranged by size:
3966 1933 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2856 3922 4683 5711 1540
Size: 275 186 855 1066 761 1028 2301
Fragments arranged by size:
2301 1066 1028 855 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2470 3260 4533 4618 5689 2470
Size: 790 1273 85 1071 3253
Fragments arranged by size:
3253 1273 1071 790 85
BsmI GAATG_Cn'
Cuts at: 2771 3983 4076 2771
Size: 1212 93 5167
Fragments arranged by size:
5167 1212 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2470 2513 3260 3791 4533 4618 5689
Size: 399 680 43 747 531 742 85 1071
Cuts at: 5689 1391
Size: 2174
Fragments arranged by size:
2174 1071 747 742 680 531 399 85
43
BspEI T'CCGG_A
Cuts at: 6135 6135
Size: 6472
BspHI T'CATG_A
Cuts at: 4361 6091 4361
Size: 1730 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3827 844
Size: 2983 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4359 1394
Size: 429 2536 3507
Fragments arranged by size:
3507 2536 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2689 4967 5141 65
Size: 1183 1441 2278 174 1396
Fragments arranged by size:
2278 1441 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5116 5303 1700
Size: 110 3306 187 2869
Fragments arranged by size:
3306 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6472
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 4532 5544 4532
Size: 1012 5460
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2474 3113 794
Size: 1680 639 4153
Fragments arranged by size:
4153 1680 639
BstBI TT'CG_AA
Cuts at: 5276 5276
Size: 6472
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6472
BstYI r'GATC_y
Cuts at: 1972 3802 4169 4554 4571 6023 6109 6121 6467
Size: 1830 367 385 17 1452 86 12 346
Cuts at: 6467 1972
Size: 1977
Fragments arranged by size:
1977 1830 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2580 2580
Size: 6472
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5920
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3658 487
Size: 683 56 2432 3301
Fragments arranged by size:
3301 2432 683 56
BtsI GCAGTG_nn'
Cuts at: 3988 4782 4802 3988
Size: 794 20 5658
Fragments arranged by size:
5658 794 20
ClaI AT'CG_AT
Cuts at: 1554 2407 1554
Size: 853 5619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6437
DraI TTT'AAA
Cuts at: 3012 3776 3012
Size: 764 5708
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5479 809
Size: 507 4163 1802
Fragments arranged by size:
4163 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3919 4808 6437
Size: 54 1425 50 275 18 2089 889 1629
Cuts at: 6437 8
Size: 43
Fragments arranged by size:
2089 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3919 1537
Size: 275 2107 4090
Fragments arranged by size:
4090 2107 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2334 4402 1353
Size: 689 292 2068 3423
Fragments arranged by size:
3423 2068 689 292
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5059 5433 5579 387
Size: 1064 138 3470 374 146 1280
Fragments arranged by size:
3470 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2240 2268 2414 2523 2886 2958
Size: 393 491 117 28 146 109 363 72
Cuts at: 2958 3255 3401 4535 5132 5919 6187 1239
Size: 297 146 1134 597 787 268 1524
Fragments arranged by size:
1524 1134 787 597 491 393 363 297
268 146 146 117 109 72 28
EcoO109I rG'GnC_Cy
Cuts at: 3623 3691 3623
Size: 68 6404
EcoRI G'AATT_C
Cuts at: 3781 4159 3781
Size: 378 6094
EcoRV GAT'ATC
Cuts at: 2545 3295 2545
Size: 750 5722
FspI TGC'GCA
Cuts at: 4978 4978
Size: 6472
HaeII r_GCGC'y
Cuts at: 1678 5619 1678
Size: 3941 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2208 2240 2244 2266 2276
Size: 32 135 32 636 32 4 22 10
Cuts at: 2276 2298 2489 2521 2602 2634 3591 3623 5113
Size: 22 191 32 81 32 957 32 1490
Cuts at: 5113 5145 5187 5219 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1490 957 636 191 135 81 42
32 32 32 32 32 32 32 32
22 22 10 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3816 4062 669
Size: 988 281 1878 246 3079
Fragments arranged by size:
3079 1878 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3683 748
Size: 2935 3537
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KpnI G_GTAC'C
Cuts at: 2487 3797 2487
Size: 1310 5162
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2938 3086 3229 3739 5586 5770 1305
Size: 1633 148 143 510 1847 184 2007
Fragments arranged by size:
2007 1847 1633 510 184 148 143
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4650
Fragments arranged by size:
4650 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3087 3137 3495 4430 4789 4948 518
Size: 648 1921 50 358 935 359 159 2042
Fragments arranged by size:
2042 1921 935 648 359 358 159 50
MspA1I CmG'CkG
Cuts at: 1606 3224 3338 3888 4565 5713 5958 1606
Size: 1618 114 550 677 1148 245 2120
Fragments arranged by size:
2120 1618 1148 677 550 245 114
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5920
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 3919 3919
Size: 6472
NsiI A_TGCA'T
Cuts at: 3088 3088
Size: 6472
NspI r_CATG'y
Cuts at: 1132 1229 3090 3830 5375 1132
Size: 97 1861 740 1545 2229
Fragments arranged by size:
2229 1861 1545 740 97
PasI CC'CwG_GG
Cuts at: 1348 2047 1348
Size: 699 5773
PciI A'CATG_T
Cuts at: 5371 5371
Size: 6472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 2494 1108
Size: 939 447 5086
Fragments arranged by size:
5086 939 447
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4541 4573 6054 6086 4541
Size: 32 1481 32 4927
Fragments arranged by size:
4927 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3691 3691
Size: 6472
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 4042 4042
Size: 6472
PspOMI G'GGCC_C
Cuts at: 2509 2509
Size: 6472
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2361 2393 2767 2799 2361
Size: 32 374 32 6034
Fragments arranged by size:
6034 374 32 32
PstI C_TGCA'G
Cuts at: 830 3824 830
Size: 2994 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6472
PvuII CAG'CTG
Cuts at: 3338 3338
Size: 6472
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SapI GCTCTTCn'nnn_
Cuts at: 2042 2042
Size: 6472
SbfI CC_TGCA'GG
Cuts at: 3824 3824
Size: 6472
ScaI AGT'ACT
Cuts at: 4720 6432 4720
Size: 1712 4760
SfcI C'TryA_G
Cuts at: 826 1045 3484 3820 4955 5636 5827 826
Size: 219 2439 336 1135 681 191 1471
Fragments arranged by size:
2439 1471 1135 681 336 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 3640 3799 1872
Size: 1768 159 4545
Fragments arranged by size:
4545 1768 159
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4576 5477 5739 820
Size: 197 926 2633 901 262 1553
Fragments arranged by size:
2633 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4263 493
Size: 3770 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 3830 1132
Size: 2698 3774
SspI AAT'ATT
Cuts at: 5 52 2452 2755 2834 4396 5
Size: 47 2400 303 79 1562 2081
Fragments arranged by size:
2400 2081 1562 303 79 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6472
StyI C'CwwG_G
Cuts at: 513 1065 2571 3911 513
Size: 552 1506 1340 3074
Fragments arranged by size:
3074 1506 1340 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4499 4516 4669 4854 5299 325
Size: 1255 2919 17 153 185 445 1498
Fragments arranged by size:
2919 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4718 6430 96
Size: 274 80 33 51 1443 2741 1712 138
Fragments arranged by size:
2741 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6432 6464 6432
Size: 32 6440
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6472
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6472
XmaI C'CCGG_G
Cuts at: 1870 3638 3797 1870
Size: 1768 159 4545
Fragments arranged by size:
4545 1768 159
XmnI GAAnn'nnTTC
Cuts at: 4601 4601
Size: 6472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2203 4280 276
Size: 53 83 186 936 669 2077 2468
Fragments arranged by size:
2468 2077 936 669 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspXI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI