Restriction map for
pFN21AE5762
(Circular) (Six-base) MAPSORT of: pFN21AE5762.seq Check: 3386 from: 1 to: 7234
pFN21AE5762 7234 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4218 4218
Size: 7234
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3894 5044 278
Size: 53 83 186 936 2358 1150 2468
Fragments arranged by size:
2468 2358 1150 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4577 1937
Size: 2640 4594
Acc65I G'GTAC_C
Cuts at: 4555 4555
Size: 7234
AclI AA'CG_TT
Cuts at: 1563 5361 5734 1563
Size: 3798 373 3063
Fragments arranged by size:
3798 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2303 2906 3581 4241 5297 6681 6949 1632
Size: 671 603 675 660 1056 1384 268 1917
Fragments arranged by size:
1917 1384 1056 675 671 660 603 268
AfeI AGC'GCT
Cuts at: 3164 3164
Size: 7234
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7037
AflIII A'CryG_T
Cuts at: 1203 2620 6133 7110 1203
Size: 1417 3513 977 1327
Fragments arranged by size:
3513 1417 1327 977
AgeI A'CCGG_T
Cuts at: 6065 6065
Size: 7234
AhdI GACnn_n'nnGTC
Cuts at: 5963 5963
Size: 7234
AleI CACnn'nnGTG
Cuts at: 1166 2303 2663 2693 3339 1166
Size: 1137 360 30 646 5061
Fragments arranged by size:
5061 1137 646 360 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7234
AlwNI CAG_nnn'CTG
Cuts at: 4977 6549 4977
Size: 1572 5662
ApaI G_GGCC'C
Cuts at: 4066 4066
Size: 7234
ApaLI G'TGCA_C
Cuts at: 2606 5291 6447 2606
Size: 2685 1156 3393
Fragments arranged by size:
3393 2685 1156
ApoI r'AATT_y
Cuts at: 1493 2270 2492 4543 4769 4921 7030 1493
Size: 777 222 2051 226 152 2109 1697
Fragments arranged by size:
2109 2051 1697 777 226 222 152
AseI AT'TA_AT
Cuts at: 160 1032 5788 160
Size: 872 4756 1606
Fragments arranged by size:
4756 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
AvaI C'yCGr_G
Cuts at: 1870 1943 2372 3496 4214 4429 4527 4559 1870
Size: 73 429 1124 718 215 98 32 4545
Fragments arranged by size:
4545 1124 718 429 215 98 73 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3501 3534 3501
Size: 33 7201
BamHI G'GATC_C
Cuts at: 2208 3060 3378 3714 4564 4931 2208
Size: 852 318 336 850 367 4511
Fragments arranged by size:
4511 852 850 367 336 318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3201 3234 4016 4076 4088
Size: 325 217 627 1414 33 782 60 12
Cuts at: 4088 4555 6010 618
Size: 467 1455 1842
Fragments arranged by size:
1842 1455 1414 782 627 467 325 217
60 33 12
BanII G_rGCy'C
Cuts at: 1391 3054 4066 4494 4553 1391
Size: 1663 1012 428 59 4072
Fragments arranged by size:
4072 1663 1012 428 59
BbeI G_GCGC'C
Cuts at: 4092 4092
Size: 7234
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3122 928
Size: 971 1223 5040
Fragments arranged by size:
5040 1223 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5425 5459 1903
Size: 34 3488 34 3678
Fragments arranged by size:
3678 3488 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2856 5126 6336 2856
Size: 2270 1210 3754
Fragments arranged by size:
3754 2270 1210
BclI T'GATC_A
Cuts at: 1548 3861 1548
Size: 2313 4921
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4112 5845 136
Size: 107 122 71 631 605 2440 1733 1525
Fragments arranged by size:
2440 1733 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4002 4476 7229 4002
Size: 474 2753 4007
Fragments arranged by size:
4007 2753 474
BlpI GC'TnA_GC
Cuts at: 4651 4651
Size: 7234
Bme1580I G_kGCm'C
Cuts at: 1790 2610 2700 3807 4066 5295 6451 1790
Size: 820 90 1107 259 1229 1156 2573
Fragments arranged by size:
2573 1229 1156 1107 820 259 90
BmgBI CAC'GTC
Cuts at: 1318 2077 3073 3607 3754 1318
Size: 759 996 534 147 4798
Fragments arranged by size:
4798 996 759 534 147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2687 3097 3902
Size: 416 53 83 399 24 1270 410 805
Cuts at: 3902 4965 5923 442
Size: 1063 958 1753
Fragments arranged by size:
1753 1270 1063 958 805 416 410 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7234
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2848 2848
Size: 7234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2818 2903 3227 3449 3674 3962 4426 5894
Size: 1579 85 324 222 225 288 464 1468
Cuts at: 5894 1239
Size: 2579
Fragments arranged by size:
2579 1579 1468 464 324 288 225 222
85
Bpu10I CC'TnA_GC
Cuts at: 3048 3551 3048
Size: 503 6731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3620 5359 6224 6522 3620
Size: 1739 865 298 4332
Fragments arranged by size:
4332 1739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3777 5897 882
Size: 617 2278 2120 2219
Fragments arranged by size:
2278 2219 2120 617
BsaAI yAC'GTr
Cuts at: 493 1204 3247 5025 493
Size: 711 2043 1778 2702
Fragments arranged by size:
2702 2043 1778 711
BsaBI GATnn'nnATC
Cuts at: 7228 7228
Size: 7234
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3891 4089 5041 5423
Size: 53 83 186 936 2358 198 952 382
Cuts at: 5423 6058 275
Size: 635 1451
Fragments arranged by size:
2358 1451 952 936 635 382 198 186
83 53
BsaWI w'CCGG_w
Cuts at: 2811 4173 4380 4449 5667 6065 6339 6486 6897
Size: 1362 207 69 1218 398 274 147 411
Cuts at: 6897 2811
Size: 3148
Fragments arranged by size:
3148 1362 1218 411 398 274 207 147
69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3647 3671 3677 3701 1178
Size: 30 165 30 2244 24 6 24 4711
Fragments arranged by size:
4711 2244 165 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2552 3317 3548 3676 4124 1186
Size: 1204 162 765 231 128 448 4296
Fragments arranged by size:
4296 1204 765 448 231 162 128
BseYI C'CCAG_C
Cuts at: 6437 6933 6437
Size: 496 6738
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3734 1163
Size: 573 1998 4663
Fragments arranged by size:
4663 1998 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3984 4684 5445 6473 1540
Size: 275 186 1983 700 761 1028 2301
Fragments arranged by size:
2301 1983 1028 761 700 275 186
BsiHKAI G_wGCw'C
Cuts at: 2610 2631 3054 5295 5380 6451 2610
Size: 21 423 2241 85 1071 3393
Fragments arranged by size:
3393 2241 1071 423 85 21
BsmI GAATG_Cn'
Cuts at: 2329 4745 4838 2329
Size: 2416 93 4725
Fragments arranged by size:
4725 2416 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2981 2981
Size: 7234
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2610 2631 2700 3054 3807 4066 4494
Size: 399 820 21 69 354 753 259 428
Cuts at: 4494 4553 5295 5380 6451 1391
Size: 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 820 753 742 428 399 354
259 85 69 59 21
BspEI T'CCGG_A
Cuts at: 6897 6897
Size: 7234
BspHI T'CATG_A
Cuts at: 3901 5123 6853 3901
Size: 1222 1730 4282
Fragments arranged by size:
4282 1730 1222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2521 4218 4509 4589 844
Size: 1677 1697 291 80 3489
Fragments arranged by size:
3489 1697 1677 291 80
BsrBI CCG'CTC
Cuts at: 1394 1823 5121 1394
Size: 429 3298 3507
Fragments arranged by size:
3507 3298 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2143 5729 5903 65
Size: 1183 895 3586 174 1396
Fragments arranged by size:
3586 1396 1183 895 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 3474 5878 6065 1700
Size: 110 293 1371 2404 187 2869
Fragments arranged by size:
2869 2404 1371 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 3765 96
Size: 3669 3565
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7234
BssSI C'ACGA_G
Cuts at: 2195 3719 5294 6306 2195
Size: 1524 1575 1012 3123
Fragments arranged by size:
3123 1575 1524 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2182 794
Size: 1388 5846
BstBI TT'CG_AA
Cuts at: 6038 6038
Size: 7234
BstEII G'GTnAC_C
Cuts at: 1184 3842 3874 1184
Size: 2658 32 4544
Fragments arranged by size:
4544 2658 32
BstYI r'GATC_y
Cuts at: 1972 2208 3060 3378 3714 4002 4476 4506 4564
Size: 236 852 318 336 288 474 30 58
Cuts at: 4564 4931 5316 5333 6785 6871 6883 7229 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 852 474 385 367 346 336
318 288 236 86 58 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 3150 4027 3150
Size: 877 6357
BtgI C'CryG_G
Cuts at: 513 1065 2004 3197 4080 4230 513
Size: 552 939 1193 883 150 3517
Fragments arranged by size:
3517 1193 939 883 552 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2346 3282 4405 487
Size: 683 56 1120 936 1123 3316
Fragments arranged by size:
3316 1123 1120 936 683 56
BtsI GCAGTG_nn'
Cuts at: 4008 4252 4358 4750 5544 5564 4008
Size: 244 106 392 794 20 5678
Fragments arranged by size:
5678 794 392 244 106 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7234
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7199
DraI TTT'AAA
Cuts at: 4538 4538
Size: 7234
DraIII CAC_nnn'GTG
Cuts at: 2182 2197 3142 2182
Size: 15 945 6274
Fragments arranged by size:
6274 945 15
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6241 809
Size: 507 4925 1802
Fragments arranged by size:
4925 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2142 2673
Size: 54 1425 50 275 18 249 63 531
Cuts at: 2673 2907 3006 3249 3504 3609 3789 3852 3981
Size: 234 99 243 255 105 180 63 129
Cuts at: 3981 4104 4681 5570 7199 8
Size: 123 577 889 1629 43
Fragments arranged by size:
1629 1425 889 577 531 275 255 249
243 234 180 129 123 105 99 63
63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3981 4681 1537
Size: 275 2169 700 4090
Fragments arranged by size:
4090 2169 700 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 3704 4136 5164 1353
Size: 734 1617 432 1028 3423
Fragments arranged by size:
3423 1617 1028 734 432
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2731 2921 5821 6195 6341 387
Size: 1064 138 1142 190 2900 374 146 1280
Fragments arranged by size:
2900 1280 1142 1064 374 190 146 138
EcoICRI GAG'CTC
Cuts at: 3052 3052
Size: 7234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2818 2903 2906 3227 3449 3581
Size: 393 671 515 85 3 321 222 132
Cuts at: 3581 3674 3962 4241 4426 5297 5894 6681 6949
Size: 93 288 279 185 871 597 787 268
Cuts at: 6949 1239
Size: 1524
Fragments arranged by size:
1524 871 787 671 597 515 393 321
288 279 268 222 185 132 93 85
3
EcoNI CCTnn'n_nnAGG
Cuts at: 3656 3656
Size: 7234
EcoO109I rG'GnC_Cy
Cuts at: 2839 2965 3351 4062 4063 2839
Size: 126 386 711 1 6010
Fragments arranged by size:
6010 711 386 126 1
EcoRI G'AATT_C
Cuts at: 2270 4543 4921 2270
Size: 2273 378 4583
Fragments arranged by size:
4583 2273 378
FspI TGC'GCA
Cuts at: 3284 5740 3284
Size: 2456 4778
HaeII r_GCGC'y
Cuts at: 1678 3166 3839 4092 6381 1678
Size: 1488 673 253 2289 2531
Fragments arranged by size:
2531 2289 1488 673 253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2778 2810 5875 5907 5949
Size: 32 135 32 1206 32 3065 32 42
Cuts at: 5949 5981 1373
Size: 32 2626
Fragments arranged by size:
3065 2626 1206 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2722 4578 4824 669
Size: 988 281 784 1856 246 3079
Fragments arranged by size:
3079 1856 988 784 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7234
HpaI GTT'AAC
Cuts at: 4824 4824
Size: 7234
KasI G'GCGC_C
Cuts at: 4088 4088
Size: 7234
KpnI G_GTAC'C
Cuts at: 4559 4559
Size: 7234
MfeI C'AATT_G
Cuts at: 4833 4833
Size: 7234
MluI A'CGCG_T
Cuts at: 2620 7110 2620
Size: 4490 2744
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4521 6348 6532 1305
Size: 3216 1827 184 2007
Fragments arranged by size:
3216 2007 1827 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 2675 3008 3611 3854
Size: 54 1425 343 312 531 333 603 243
Cuts at: 3854 10
Size: 3390
Fragments arranged by size:
3390 1425 603 531 343 333 312 243
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 2663 2693 3339 4301 5192 5551
Size: 648 1137 360 30 646 962 891 359
Cuts at: 5551 5710 518
Size: 159 2042
Fragments arranged by size:
2042 1137 962 891 648 646 360 359
159 30
MspA1I CmG'CkG
Cuts at: 1606 2017 2205 3016 3199 3232 3559 3577 3736
Size: 411 188 811 183 33 327 18 159
Cuts at: 3736 3986 4375 4650 5327 6475 6720 1606
Size: 250 389 275 677 1148 245 2120
Fragments arranged by size:
2120 1148 811 677 411 389 327 275
250 245 188 183 159 33 18
NarI GG'CG_CC
Cuts at: 4089 4089
Size: 7234
NcoI C'CATG_G
Cuts at: 513 1065 2004 4080 513
Size: 552 939 2076 3667
Fragments arranged by size:
3667 2076 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7234
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7234
NotI GC'GGCC_GC
Cuts at: 4681 4681
Size: 7234
NspI r_CATG'y
Cuts at: 1132 1229 3707 4300 4351 4592 6137 1132
Size: 97 2478 593 51 241 1545 2229
Fragments arranged by size:
2478 2229 1545 593 241 97 51
PasI CC'CwG_GG
Cuts at: 1348 2571 1348
Size: 1223 6011
PciI A'CATG_T
Cuts at: 6133 6133
Size: 7234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2197 4395 1108
Size: 1089 2198 3947
Fragments arranged by size:
3947 2198 1089
PfoI T'CCnGG_A
Cuts at: 2805 2919 3651 3939 2805
Size: 114 732 288 6100
Fragments arranged by size:
6100 732 288 114
PmeI GTTT'AAAC
Cuts at: 4538 4538
Size: 7234
PmlI CAC'GTG
Cuts at: 3247 3247
Size: 7234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5303 5335 6816 6848 5303
Size: 32 1481 32 5689
Fragments arranged by size:
5689 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2839 2965 3351 2839
Size: 126 386 6722
Fragments arranged by size:
6722 386 126
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7234
PsiI TTA'TAA
Cuts at: 4804 4804
Size: 7234
PspOMI G'GGCC_C
Cuts at: 4062 4062
Size: 7234
PspXI vC'TCGA_Gb
Cuts at: 2372 2372
Size: 7234
PstI C_TGCA'G
Cuts at: 830 2295 2772 4015 4506 4586 830
Size: 1465 477 1243 491 80 3478
Fragments arranged by size:
3478 1465 1243 491 477 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7234
PvuII CAG'CTG
Cuts at: 3016 3559 3736 4375 3016
Size: 543 177 639 5875
Fragments arranged by size:
5875 639 543 177
SacI G_AGCT'C
Cuts at: 3054 3054
Size: 7234
SacII CC_GC'GG
Cuts at: 3200 3200
Size: 7234
SalI G'TCGA_C
Cuts at: 1936 4576 1936
Size: 2640 4594
SanDI GG'GwC_CC
Cuts at: 2965 3351 2965
Size: 386 6848
SapI GCTCTTCn'nnn_
Cuts at: 2087 2087
Size: 7234
SbfI CC_TGCA'GG
Cuts at: 4586 4586
Size: 7234
ScaI AGT'ACT
Cuts at: 2897 4199 5482 7194 2897
Size: 1302 1283 1712 2937
Fragments arranged by size:
2937 1712 1302 1283
SfcI C'TryA_G
Cuts at: 826 1045 2291 2768 4011 4502 4582 5717 6398
Size: 219 1246 477 1243 491 80 1135 681
Cuts at: 6398 6589 826
Size: 191 1471
Fragments arranged by size:
1471 1246 1243 1135 681 491 477 219
191 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 4112 4112
Size: 7234
SfoI GGC'GCC
Cuts at: 4090 4090
Size: 7234
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
SgrAI Cr'CCGG_yG
Cuts at: 1700 3474 1700
Size: 1774 5460
SmaI CCC'GGG
Cuts at: 1872 3498 4529 4561 1872
Size: 1626 1031 32 4545
Fragments arranged by size:
4545 1626 1031 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2372 3635 5338 6239 6501 820
Size: 197 926 429 1263 1703 901 262 1553
Fragments arranged by size:
1703 1553 1263 926 901 429 262 197
SnaBI TAC'GTA
Cuts at: 493 5025 493
Size: 4532 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7234
SphI G_CATG'C
Cuts at: 1132 4592 1132
Size: 3460 3774
SspI AAT'ATT
Cuts at: 5 52 5158 5
Size: 47 5106 2081
Fragments arranged by size:
5106 2081 47
StuI AGG'CCT
Cuts at: 1517 2412 3098 3155 4025 4166 1517
Size: 895 686 57 870 141 4585
Fragments arranged by size:
4585 895 870 686 141 57
StyI C'CwwG_G
Cuts at: 513 1065 2004 2398 2454 2745 3156 3612 3792
Size: 552 939 394 56 291 411 456 180
Cuts at: 3792 3960 4080 4167 4263 4389 4673 513
Size: 168 120 87 96 126 284 3074
Fragments arranged by size:
3074 939 552 456 411 394 291 284
180 168 126 120 96 87 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3041 3394 3905 5261 5278 5431 5616
Size: 1255 1461 353 511 1356 17 153 185
Cuts at: 5616 6061 325
Size: 445 1498
Fragments arranged by size:
1498 1461 1356 1255 511 445 353 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2895 3663 3723
Size: 274 80 33 51 1443 918 768 60
Cuts at: 3723 3765 4197 5480 7192 96
Size: 42 432 1283 1712 138
Fragments arranged by size:
1712 1443 1283 918 768 432 274 138
80 60 51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7194 7226 7194
Size: 32 7202
Tth111I GACn'n_nGTC
Cuts at: 1316 3893 1316
Size: 2577 4657
XbaI T'CTAG_A
Cuts at: 4570 4570
Size: 7234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 3362 4078 4099 4244 2347
Size: 1015 716 21 145 5337
Fragments arranged by size:
5337 1015 716 145 21
XhoI C'TCGA_G
Cuts at: 1943 2372 1943
Size: 429 6805
XmaI C'CCGG_G
Cuts at: 1870 3496 4527 4559 1870
Size: 1626 1031 32 4545
Fragments arranged by size:
4545 1626 1031 32
XmnI GAAnn'nnTTC
Cuts at: 2096 4130 5363 2096
Size: 2034 1233 3967
Fragments arranged by size:
3967 2034 1233
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3892 5042 276
Size: 53 83 186 936 2358 1150 2468
Fragments arranged by size:
2468 2358 1150 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BfrBI BsiWI BstXI BstZ17I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PsrI RsrII SexAI SrfI SwaI