Restriction map for
pFN21AE5757
(Circular) (Six-base) MAPSORT of: pFN21AE5757.seq Check: 5545 from: 1 to: 6982
pFN21AE5757 6982 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3967 3967
Size: 6982
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4792 278
Size: 53 83 186 936 3256 2468
Fragments arranged by size:
3256 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4325 1937
Size: 2388 4594
Acc65I G'GTAC_C
Cuts at: 4303 4303
Size: 6982
AclI AA'CG_TT
Cuts at: 1563 5109 5482 1563
Size: 3546 373 3063
Fragments arranged by size:
3546 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3266 3440 3734 3987 5045 6429 6697 1632
Size: 1634 174 294 253 1058 1384 268 1917
Fragments arranged by size:
1917 1634 1384 1058 294 268 253 174
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6785
AflIII A'CryG_T
Cuts at: 1203 5881 6858 1203
Size: 4678 977 1327
Fragments arranged by size:
4678 1327 977
AgeI A'CCGG_T
Cuts at: 5813 5813
Size: 6982
AhdI GACnn_n'nnGTC
Cuts at: 5711 5711
Size: 6982
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6982
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3122 3675 1184
Size: 1938 553 4491
Fragments arranged by size:
4491 1938 553
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 2057
Size: 32 6950
AlwNI CAG_nnn'CTG
Cuts at: 2558 2834 3471 3622 3848 4725 6297 2558
Size: 276 637 151 226 877 1572 3243
Fragments arranged by size:
3243 1572 877 637 276 226 151
ApaI G_GGCC'C
Cuts at: 2188 2188
Size: 6982
ApaLI G'TGCA_C
Cuts at: 5039 6195 5039
Size: 1156 5826
ApoI r'AATT_y
Cuts at: 1493 2802 4291 4517 4669 6778 1493
Size: 1309 1489 226 152 2109 1697
Fragments arranged by size:
2109 1697 1489 1309 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2351 5536 160
Size: 872 1319 3185 1606
Fragments arranged by size:
3185 1606 1319 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6982
AvaI C'yCGr_G
Cuts at: 1870 1943 3333 4307 1870
Size: 73 1390 974 4545
Fragments arranged by size:
4545 1390 974 73
AvrII C'CTAG_G
Cuts at: 2441 2441
Size: 6982
BamHI G'GATC_C
Cuts at: 3777 4312 4679 3777
Size: 535 367 6080
Fragments arranged by size:
6080 535 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3311 3904 4303 5758 618
Size: 325 217 627 1524 593 399 1455 1842
Fragments arranged by size:
1842 1524 1455 627 593 399 325 217
BanII G_rGCy'C
Cuts at: 1391 2188 3935 4301 1391
Size: 797 1747 366 4072
Fragments arranged by size:
4072 1747 797 366
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2402 928
Size: 971 503 5508
Fragments arranged by size:
5508 971 503
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5173 5207 1903
Size: 34 3236 34 3678
Fragments arranged by size:
3678 3236 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4874 6084 4874
Size: 1210 5772
BclI T'GATC_A
Cuts at: 1548 2848 1548
Size: 1300 5682
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2330 5593 136
Size: 107 122 71 631 605 658 3263 1525
Fragments arranged by size:
3263 1525 658 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6977 6977
Size: 6982
BlpI GC'TnA_GC
Cuts at: 4399 4399
Size: 6982
Bme1580I G_kGCm'C
Cuts at: 1790 2188 2297 3909 5043 6199 1790
Size: 398 109 1612 1134 1156 2573
Fragments arranged by size:
2573 1612 1156 1134 398 109
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6982
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3142 4713 5671
Size: 416 53 83 399 24 1725 1571 958
Cuts at: 5671 442
Size: 1753
Fragments arranged by size:
1753 1725 1571 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 3180 5642 1239
Size: 845 1096 2462 2579
Fragments arranged by size:
2579 2462 1096 845
Bpu10I CC'TnA_GC
Cuts at: 2366 4167 2366
Size: 1801 5181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 3144 3224 3419 5107 5972 6270 2174
Size: 970 80 195 1688 865 298 2886
Fragments arranged by size:
2886 1688 970 865 298 195 80
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2818 3576 3712 5645 882
Size: 617 1319 758 136 1933 2219
Fragments arranged by size:
2219 1933 1319 758 617 136
BsaAI yAC'GTr
Cuts at: 493 1204 4773 493
Size: 711 3569 2702
Fragments arranged by size:
3569 2702 711
BsaBI GATnn'nnATC
Cuts at: 6976 6976
Size: 6982
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4789 5171 5806 275
Size: 53 83 186 936 3256 382 635 1451
Fragments arranged by size:
3256 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5415 5813 6087 6234 6645 5415
Size: 398 274 147 411 5752
Fragments arranged by size:
5752 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2057 2087 1178
Size: 30 165 30 654 30 6073
Fragments arranged by size:
6073 654 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2873 2948 4276 1186
Size: 1687 75 1328 3892
Fragments arranged by size:
3892 1687 1328 75
BseYI C'CCAG_C
Cuts at: 3353 6185 6681 3353
Size: 2832 496 3654
Fragments arranged by size:
3654 2832 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3461 1163
Size: 573 1725 4684
Fragments arranged by size:
4684 1725 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4432 5193 6221 1540
Size: 275 186 2431 761 1028 2301
Fragments arranged by size:
2431 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3654 4249 5043 5128 6199 3654
Size: 595 794 85 1071 4437
Fragments arranged by size:
4437 1071 794 595 85
BsmI GAATG_Cn'
Cuts at: 3124 4493 4586 3124
Size: 1369 93 5520
Fragments arranged by size:
5520 1369 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2297 3654 3909 3935 4249 4301
Size: 399 398 109 1357 255 26 314 52
Cuts at: 4301 5043 5128 6199 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1357 1071 742 399 398 314 255
109 85 52 26
BspEI T'CCGG_A
Cuts at: 6645 6645
Size: 6982
BspHI T'CATG_A
Cuts at: 3153 4871 6601 3153
Size: 1718 1730 3534
Fragments arranged by size:
3534 1730 1718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2495 3967 4138 4337 844
Size: 1651 1472 171 199 3489
Fragments arranged by size:
3489 1651 1472 199 171
BsrBI CCG'CTC
Cuts at: 1394 1823 4869 1394
Size: 429 3046 3507
Fragments arranged by size:
3507 3046 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5477 5651 65
Size: 1183 4229 174 1396
Fragments arranged by size:
4229 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2089 3231 5626 5813 1700
Size: 110 199 80 1142 2395 187 2869
Fragments arranged by size:
2869 2395 1142 199 187 110 80
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6982
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6982
BssSI C'ACGA_G
Cuts at: 3653 5042 6054 3653
Size: 1389 1012 4581
Fragments arranged by size:
4581 1389 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4253 794
Size: 3459 3523
BstBI TT'CG_AA
Cuts at: 5786 5786
Size: 6982
BstEII G'GTnAC_C
Cuts at: 1184 2785 3256 1184
Size: 1601 471 4910
Fragments arranged by size:
4910 1601 471
BstXI CCAn_nnnn'nTGG
Cuts at: 3077 3077
Size: 6982
BstYI r'GATC_y
Cuts at: 1972 3777 3803 4312 4679 5064 5081 6533 6619
Size: 1805 26 509 367 385 17 1452 86
Cuts at: 6619 6631 6977 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1805 1452 509 385 367 346 86
26 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2571 513
Size: 552 939 567 4924
Fragments arranged by size:
4924 939 567 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6243
Fragments arranged by size:
6243 683 56
BtsI GCAGTG_nn'
Cuts at: 2059 2105 2851 4498 5292 5312 2059
Size: 46 746 1647 794 20 3729
Fragments arranged by size:
3729 1647 794 746 46 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6982
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6947
DraI TTT'AAA
Cuts at: 4286 4286
Size: 6982
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5989 809
Size: 507 4673 1802
Fragments arranged by size:
4673 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3229 3393
Size: 54 1425 50 275 18 177 1222 164
Cuts at: 3393 4429 5318 6947 8
Size: 1036 889 1629 43
Fragments arranged by size:
1629 1425 1222 1036 889 275 177 164
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4429 1537
Size: 275 2617 4090
Fragments arranged by size:
4090 2617 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2673 2685 2754 2760 2804 2969 3710 3914
Size: 1320 12 69 6 44 165 741 204
Cuts at: 3914 3995 4912 1353
Size: 81 917 3423
Fragments arranged by size:
3423 1320 917 741 204 165 81 69
44 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3665 5569 5943 6089 387
Size: 1064 138 2076 1904 374 146 1280
Fragments arranged by size:
2076 1904 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 3180 3266 3440 3734 3987 5045
Size: 393 452 1096 86 174 294 253 1058
Cuts at: 5045 5642 6429 6697 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1096 1058 787 597 452 393 294
268 253 174 86
EcoNI CCTnn'n_nnAGG
Cuts at: 4239 4239
Size: 6982
EcoO109I rG'GnC_Cy
Cuts at: 2184 2185 4012 4037 4232 2184
Size: 1 1827 25 195 4934
Fragments arranged by size:
4934 1827 195 25 1
EcoRI G'AATT_C
Cuts at: 4291 4669 4291
Size: 378 6604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 3071 3265 2119
Size: 952 194 5836
Fragments arranged by size:
5836 952 194
FseI GG_CCGG'CC
Cuts at: 2013 2013
Size: 6982
FspI TGC'GCA
Cuts at: 3557 5488 3557
Size: 1931 5051
FspAI rTGC'GCAy
Cuts at: 3557 3557
Size: 6982
HaeII r_GCGC'y
Cuts at: 1678 4022 6129 1678
Size: 2344 2107 2531
Fragments arranged by size:
2531 2344 2107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 4136 4168 5623
Size: 32 135 32 1013 32 1519 32 1455
Cuts at: 5623 5655 5697 5729 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1519 1455 1013 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3247 4326 4572 669
Size: 988 281 1309 1079 246 3079
Fragments arranged by size:
3079 1309 1079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3278 748
Size: 2530 4452
HpaI GTT'AAC
Cuts at: 4572 4572
Size: 6982
KpnI G_GTAC'C
Cuts at: 4307 4307
Size: 6982
MfeI C'AATT_G
Cuts at: 4581 4581
Size: 6982
MluI A'CGCG_T
Cuts at: 6858 6858
Size: 6982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3094 6096 6280 1305
Size: 1789 3002 184 2007
Fragments arranged by size:
3002 2007 1789 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3395 10
Size: 54 1425 343 1563 3597
Fragments arranged by size:
3597 1563 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 3657 4940 5299 5458 518
Size: 648 1362 1129 1283 359 159 2042
Fragments arranged by size:
2042 1362 1283 1129 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 4201 4398 5075 6223 6468 1606
Size: 456 2139 197 677 1148 245 2120
Fragments arranged by size:
2139 2120 1148 677 456 245 197
NaeI GCC'GGC
Cuts at: 2011 3233 2011
Size: 1222 5760
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5491
Fragments arranged by size:
5491 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6982
NgoMIV G'CCGG_C
Cuts at: 2009 3231 2009
Size: 1222 5760
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6982
NotI GC'GGCC_GC
Cuts at: 4429 4429
Size: 6982
NspI r_CATG'y
Cuts at: 1132 1229 2136 3662 4340 5885 1132
Size: 97 907 1526 678 1545 2229
Fragments arranged by size:
2229 1545 1526 907 678 97
PasI CC'CwG_GG
Cuts at: 1348 3622 1348
Size: 2274 4708
PciI A'CATG_T
Cuts at: 5881 5881
Size: 6982
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6982
PfoI T'CCnGG_A
Cuts at: 2424 2424
Size: 6982
PmeI GTTT'AAAC
Cuts at: 4286 4286
Size: 6982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2057 2089 5051 5083 6564 6596 2057
Size: 32 2962 32 1481 32 2443
Fragments arranged by size:
2962 2443 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4012 4232 4012
Size: 220 6762
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6982
PsiI TTA'TAA
Cuts at: 4552 4552
Size: 6982
PspOMI G'GGCC_C
Cuts at: 2184 2184
Size: 6982
PstI C_TGCA'G
Cuts at: 830 3370 4334 830
Size: 2540 964 3478
Fragments arranged by size:
3478 2540 964
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6982
PvuII CAG'CTG
Cuts at: 2062 4201 2062
Size: 2139 4843
SalI G'TCGA_C
Cuts at: 1936 4324 1936
Size: 2388 4594
SbfI CC_TGCA'GG
Cuts at: 4334 4334
Size: 6982
ScaI AGT'ACT
Cuts at: 5230 6942 5230
Size: 1712 5270
SfcI C'TryA_G
Cuts at: 826 1045 2613 3366 3495 4330 5465 6146 6337
Size: 219 1568 753 129 835 1135 681 191
Cuts at: 6337 826
Size: 1471
Fragments arranged by size:
1568 1471 1135 835 753 681 219 191
129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6982
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6982
SmaI CCC'GGG
Cuts at: 1872 4309 1872
Size: 2437 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 3159 3239 3434 5086 5987
Size: 197 926 246 970 80 195 1652 901
Cuts at: 5987 6249 820
Size: 262 1553
Fragments arranged by size:
1652 1553 970 926 901 262 246 197
195 80
SnaBI TAC'GTA
Cuts at: 493 4773 493
Size: 4280 2702
SpeI A'CTAG_T
Cuts at: 152 3536 152
Size: 3384 3598
SphI G_CATG'C
Cuts at: 1132 3662 4340 1132
Size: 2530 678 3774
Fragments arranged by size:
3774 2530 678
SspI AAT'ATT
Cuts at: 5 52 4906 5
Size: 47 4854 2081
Fragments arranged by size:
4854 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6982
StyI C'CwwG_G
Cuts at: 513 1065 2004 2441 3960 4421 513
Size: 552 939 437 1519 461 3074
Fragments arranged by size:
3074 1519 939 552 461 437
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5009 5026 5179 5364 5809 325
Size: 1255 3429 17 153 185 445 1498
Fragments arranged by size:
3429 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5228 6940 96
Size: 274 80 33 51 1443 3251 1712 138
Fragments arranged by size:
3251 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6942 6974 6942
Size: 32 6950
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6982
XbaI T'CTAG_A
Cuts at: 4318 4318
Size: 6982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3967 3967
Size: 6982
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6982
XmaI C'CCGG_G
Cuts at: 1870 4307 1870
Size: 2437 4545
XmnI GAAnn'nnTTC
Cuts at: 2175 5111 2175
Size: 2936 4046
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4790 276
Size: 53 83 186 936 3256 2468
Fragments arranged by size:
3256 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI