Restriction map for
pFN21AE5753
(Circular) (Six-base) MAPSORT of: pFN21AE5753.seq Check: 7755 from: 1 to: 6880
pFN21AE5753 6880 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2053 2053
Size: 6880
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4690 278
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2151 4223 1937
Size: 214 2072 4594
Fragments arranged by size:
4594 2072 214
Acc65I G'GTAC_C
Cuts at: 4201 4201
Size: 6880
AclI AA'CG_TT
Cuts at: 1563 5007 5380 1563
Size: 3444 373 3063
Fragments arranged by size:
3444 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3233 3848 4943 6327 6595 1632
Size: 1601 615 1095 1384 268 1917
Fragments arranged by size:
1917 1601 1384 1095 615 268
AflII C'TTAA_G
Cuts at: 820 1017 3089 820
Size: 197 2072 4611
Fragments arranged by size:
4611 2072 197
AflIII A'CryG_T
Cuts at: 1203 3143 5779 6756 1203
Size: 1940 2636 977 1327
Fragments arranged by size:
2636 1940 1327 977
AgeI A'CCGG_T
Cuts at: 5711 5711
Size: 6880
AhdI GACnn_n'nnGTC
Cuts at: 5609 5609
Size: 6880
AleI CACnn'nnGTG
Cuts at: 1166 2203 1166
Size: 1037 5843
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6880
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2328 2360 4000 4032 2328
Size: 32 1640 32 5176
Fragments arranged by size:
5176 1640 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3323 3568 3649 3992 4623 6195 3323
Size: 245 81 343 631 1572 4008
Fragments arranged by size:
4008 1572 631 343 245 81
ApaLI G'TGCA_C
Cuts at: 4937 6093 4937
Size: 1156 5724
ApoI r'AATT_y
Cuts at: 1493 2218 2443 3982 4189 4415 4567 6676 1493
Size: 725 225 1539 207 226 152 2109 1697
Fragments arranged by size:
2109 1697 1539 725 226 225 207 152
AseI AT'TA_AT
Cuts at: 160 1032 5434 160
Size: 872 4402 1606
Fragments arranged by size:
4402 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
AvaI C'yCGr_G
Cuts at: 1870 1943 4205 1870
Size: 73 2262 4545
Fragments arranged by size:
4545 2262 73
AvrII C'CTAG_G
Cuts at: 2425 3104 2425
Size: 679 6201
BamHI G'GATC_C
Cuts at: 2359 4210 4577 2359
Size: 1851 367 4662
Fragments arranged by size:
4662 1851 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3100 3300 3474 3754 4201
Size: 325 217 627 1313 200 174 280 447
Cuts at: 4201 5656 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1313 627 447 325 280 217
200 174
BanII G_rGCy'C
Cuts at: 1391 3609 4199 1391
Size: 2218 590 4072
Fragments arranged by size:
4072 2218 590
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3239 3947 928
Size: 971 1340 708 3861
Fragments arranged by size:
3861 1340 971 708
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5071 5105 1903
Size: 34 3134 34 3678
Fragments arranged by size:
3678 3134 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 3283 4772 5982 2002
Size: 1281 1489 1210 2900
Fragments arranged by size:
2900 1489 1281 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6880
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2562 3318 5491
Size: 107 122 71 631 605 890 756 2173
Cuts at: 5491 136
Size: 1525
Fragments arranged by size:
2173 1525 890 756 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2117 6875 2117
Size: 4758 2122
BlpI GC'TnA_GC
Cuts at: 2454 2667 4297 2454
Size: 213 1630 5037
Fragments arranged by size:
5037 1630 213
Bme1580I G_kGCm'C
Cuts at: 1790 3103 3477 3757 4941 6097 1790
Size: 1313 374 280 1184 1156 2573
Fragments arranged by size:
2573 1313 1184 1156 374 280
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6880
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2106 4611 5569
Size: 416 53 83 399 24 689 2505 958
Cuts at: 5569 442
Size: 1753
Fragments arranged by size:
2505 1753 958 689 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6880
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 6880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2064 2858 3718 3789 3907 4072 5540 1239
Size: 825 794 860 71 118 165 1468 2579
Fragments arranged by size:
2579 1468 860 825 794 165 118 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2316 3243 5005 5870 6168 2316
Size: 927 1762 865 298 3028
Fragments arranged by size:
3028 1762 927 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2609 3206 3374 3410 3761 5543 882
Size: 617 1110 597 168 36 351 1782 2219
Fragments arranged by size:
2219 1782 1110 617 597 351 168 36
BsaAI yAC'GTr
Cuts at: 493 1204 3144 4671 493
Size: 711 1940 1527 2702
Fragments arranged by size:
2702 1940 1527 711
BsaBI GATnn'nnATC
Cuts at: 6874 6874
Size: 6880
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4687 5069 5704 275
Size: 53 83 186 936 3154 382 635 1451
Fragments arranged by size:
3154 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5313 5711 5985 6132 6543 5313
Size: 398 274 147 411 5650
Fragments arranged by size:
5650 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3218 3248 1178
Size: 30 165 30 1815 30 4810
Fragments arranged by size:
4810 1815 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2627 3355 3481 3521 1186
Size: 1441 728 126 40 4545
Fragments arranged by size:
4545 1441 728 126 40
BseYI C'CCAG_C
Cuts at: 2459 2636 6083 6579 2459
Size: 177 3447 496 2760
Fragments arranged by size:
3447 2760 496 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2558 1163
Size: 573 822 5485
Fragments arranged by size:
5485 822 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4330 5091 6119 1540
Size: 275 186 2329 761 1028 2301
Fragments arranged by size:
2329 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4941 5026 6097 4941
Size: 85 1071 5724
Fragments arranged by size:
5724 1071 85
BsmI GAATG_Cn'
Cuts at: 2171 3439 4391 4484 2171
Size: 1268 952 93 4567
Fragments arranged by size:
4567 1268 952 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3103 3477 3609 3757 4199 4941 5026
Size: 399 1313 374 132 148 442 742 85
Cuts at: 5026 6097 1391
Size: 1071 2174
Fragments arranged by size:
2174 1313 1071 742 442 399 374 148
132 85
BspEI T'CCGG_A
Cuts at: 6543 6543
Size: 6880
BspHI T'CATG_A
Cuts at: 3130 4769 6499 3130
Size: 1639 1730 3511
Fragments arranged by size:
3511 1730 1639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 2489 4235 844
Size: 1209 436 1746 3489
Fragments arranged by size:
3489 1746 1209 436
BsrBI CCG'CTC
Cuts at: 1394 1823 4767 1394
Size: 429 2944 3507
Fragments arranged by size:
3507 2944 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2325 5375 5549 65
Size: 1183 1077 3050 174 1396
Fragments arranged by size:
3050 1396 1183 1077 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5524 5711 1700
Size: 110 3714 187 2869
Fragments arranged by size:
3714 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6880
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6880
BssSI C'ACGA_G
Cuts at: 4940 5952 4940
Size: 1012 5868
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6880
BstBI TT'CG_AA
Cuts at: 5684 5684
Size: 6880
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6880
BstXI CCAn_nnnn'nTGG
Cuts at: 2862 3365 3782 2862
Size: 503 417 5960
Fragments arranged by size:
5960 503 417
BstYI r'GATC_y
Cuts at: 1972 2117 2359 3007 3988 4210 4577 4962 4979
Size: 145 242 648 981 222 367 385 17
Cuts at: 4979 6431 6517 6529 6875 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 981 648 385 367 346 242
222 145 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3968 3968
Size: 6880
BtgI C'CryG_G
Cuts at: 513 1065 2004 2813 3594 513
Size: 552 939 809 781 3799
Fragments arranged by size:
3799 939 809 781 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3713 487
Size: 683 56 2487 3654
Fragments arranged by size:
3654 2487 683 56
BtsI GCAGTG_nn'
Cuts at: 2614 3831 4396 5190 5210 2614
Size: 1217 565 794 20 4284
Fragments arranged by size:
4284 1217 794 565 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6880
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6845
DraI TTT'AAA
Cuts at: 2592 4184 2592
Size: 1592 5288
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5887 809
Size: 507 4571 1802
Fragments arranged by size:
4571 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2810 4327 5216
Size: 54 1425 50 275 18 980 1517 889
Cuts at: 5216 6845 8
Size: 1629 43
Fragments arranged by size:
1629 1517 1425 980 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4327 1537
Size: 275 2515 4090
Fragments arranged by size:
4090 2515 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2314 2761 2862 3499 4018 4132 4810 1353
Size: 961 447 101 637 519 114 678 3423
Fragments arranged by size:
3423 961 678 637 519 447 114 101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5467 5841 5987 387
Size: 1064 138 3878 374 146 1280
Fragments arranged by size:
3878 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2858 3233 3718 3789 3848 3907
Size: 393 432 794 375 485 71 59 59
Cuts at: 3907 4072 4943 5540 6327 6595 1239
Size: 165 871 597 787 268 1524
Fragments arranged by size:
1524 871 794 787 597 485 432 393
375 268 165 71 59 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2404 3108 3996 2404
Size: 704 888 5288
Fragments arranged by size:
5288 888 704
EcoO109I rG'GnC_Cy
Cuts at: 3859 4002 3859
Size: 143 6737
EcoRI G'AATT_C
Cuts at: 2218 3982 4189 4567 2218
Size: 1764 207 378 4531
Fragments arranged by size:
4531 1764 378 207
FspI TGC'GCA
Cuts at: 5386 5386
Size: 6880
HaeII r_GCGC'y
Cuts at: 1678 6027 1678
Size: 4349 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2963 2984 2995 3016 4131
Size: 32 135 32 1391 21 11 21 1115
Cuts at: 4131 4163 5521 5553 5595 5627 1373
Size: 32 1358 32 42 32 2626
Fragments arranged by size:
2626 1391 1358 1115 135 42 32 32
32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 4224 4470 669
Size: 988 281 2286 246 3079
Fragments arranged by size:
3079 2286 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2129 748
Size: 1381 5499
HpaI GTT'AAC
Cuts at: 4470 4470
Size: 6880
KpnI G_GTAC'C
Cuts at: 4205 4205
Size: 6880
MfeI C'AATT_G
Cuts at: 4479 4479
Size: 6880
MluI A'CGCG_T
Cuts at: 6756 6756
Size: 6880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5994 6178 1305
Size: 4689 184 2007
Fragments arranged by size:
4689 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2812 10
Size: 54 1425 343 980 4078
Fragments arranged by size:
4078 1425 980 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2203 3144 4838 5197 5356 518
Size: 648 1037 941 1694 359 159 2042
Fragments arranged by size:
2042 1694 1037 941 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2341 4296 4973 6121 6366 1606
Size: 735 1955 677 1148 245 2120
Fragments arranged by size:
2120 1955 1148 735 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2813 3594 513
Size: 552 939 809 781 3799
Fragments arranged by size:
3799 939 809 781 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6880
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6880
NotI GC'GGCC_GC
Cuts at: 4327 4327
Size: 6880
NspI r_CATG'y
Cuts at: 1132 1229 4238 5783 1132
Size: 97 3009 1545 2229
Fragments arranged by size:
3009 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6880
PciI A'CATG_T
Cuts at: 5779 5779
Size: 6880
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6880
PfoI T'CCnGG_A
Cuts at: 3884 3884
Size: 6880
PmeI GTTT'AAAC
Cuts at: 4184 4184
Size: 6880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4949 4981 6462 6494 4949
Size: 32 1481 32 5335
Fragments arranged by size:
5335 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4002 4002
Size: 6880
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6880
PsiI TTA'TAA
Cuts at: 4450 4450
Size: 6880
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3412 3444 3412
Size: 32 6848
PstI C_TGCA'G
Cuts at: 830 3328 4167 4232 830
Size: 2498 839 65 3478
Fragments arranged by size:
3478 2498 839 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6880
PvuII CAG'CTG
Cuts at: 2341 2341
Size: 6880
SalI G'TCGA_C
Cuts at: 1936 4222 1936
Size: 2286 4594
SanDI GG'GwC_CC
Cuts at: 4002 4002
Size: 6880
SbfI CC_TGCA'GG
Cuts at: 4232 4232
Size: 6880
ScaI AGT'ACT
Cuts at: 3022 5128 6840 3022
Size: 2106 1712 3062
Fragments arranged by size:
3062 2106 1712
SfcI C'TryA_G
Cuts at: 826 1045 2431 2620 3324 4163 4228 5363 6044
Size: 219 1386 189 704 839 65 1135 681
Cuts at: 6044 6235 826
Size: 191 1471
Fragments arranged by size:
1471 1386 1135 839 704 681 219 191
189 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6880
SmaI CCC'GGG
Cuts at: 1872 4207 1872
Size: 2335 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2331 3089 3258 4984 5885 6147
Size: 197 926 388 758 169 1726 901 262
Cuts at: 6147 820
Size: 1553
Fragments arranged by size:
1726 1553 926 901 758 388 262 197
169
SnaBI TAC'GTA
Cuts at: 493 4671 493
Size: 4178 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6880
SphI G_CATG'C
Cuts at: 1132 4238 1132
Size: 3106 3774
SspI AAT'ATT
Cuts at: 5 52 4804 5
Size: 47 4752 2081
Fragments arranged by size:
4752 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6880
StyI C'CwwG_G
Cuts at: 513 1065 2004 2425 2560 2813 3104 3594 3679
Size: 552 939 421 135 253 291 490 85
Cuts at: 3679 4319 513
Size: 640 3074
Fragments arranged by size:
3074 939 640 552 490 421 291 253
135 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3046 3318 3524 4907 4924 5077 5262
Size: 1255 1466 272 206 1383 17 153 185
Cuts at: 5262 5707 325
Size: 445 1498
Fragments arranged by size:
1498 1466 1383 1255 445 272 206 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2708 3020 3690
Size: 274 80 33 51 1443 731 312 670
Cuts at: 3690 5126 6838 96
Size: 1436 1712 138
Fragments arranged by size:
1712 1443 1436 731 670 312 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2674 2706 3467 3499 3866 3898 6840 6872 2674
Size: 32 761 32 367 32 2942 32 2682
Fragments arranged by size:
2942 2682 761 367 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6880
XbaI T'CTAG_A
Cuts at: 4216 4216
Size: 6880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4047 4047
Size: 6880
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6880
XmaI C'CCGG_G
Cuts at: 1870 4205 1870
Size: 2335 4545
XmnI GAAnn'nnTTC
Cuts at: 5009 5009
Size: 6880
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4688 276
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI