Restriction map for
pFN21AE5751
(Circular) (Six-base) MAPSORT of: pFN21AE5751.seq Check: 1231 from: 1 to: 6829
pFN21AE5751 6829 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3130 3130
Size: 6829
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4639 278
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2274 4172 1937
Size: 337 1898 4594
Fragments arranged by size:
4594 1898 337
Acc65I G'GTAC_C
Cuts at: 4150 4150
Size: 6829
AclI AA'CG_TT
Cuts at: 1563 4956 5329 1563
Size: 3393 373 3063
Fragments arranged by size:
3393 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3723 3842 4892 6276 6544 1632
Size: 2091 119 1050 1384 268 1917
Fragments arranged by size:
2091 1917 1384 1050 268 119
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6632
AflIII A'CryG_T
Cuts at: 1203 5728 6705 1203
Size: 4525 977 1327
Fragments arranged by size:
4525 1327 977
AgeI A'CCGG_T
Cuts at: 5660 5660
Size: 6829
AhdI GACnn_n'nnGTC
Cuts at: 2291 5558 2291
Size: 3267 3562
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6829
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6829
AlwNI CAG_nnn'CTG
Cuts at: 3347 4572 6144 3347
Size: 1225 1572 4032
Fragments arranged by size:
4032 1572 1225
ApaI G_GGCC'C
Cuts at: 3403 3403
Size: 6829
ApaLI G'TGCA_C
Cuts at: 2363 4886 6042 2363
Size: 2523 1156 3150
Fragments arranged by size:
3150 2523 1156
ApoI r'AATT_y
Cuts at: 1493 2131 2574 2961 3650 3815 4138 4364 4516
Size: 638 443 387 689 165 323 226 152
Cuts at: 4516 6625 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 689 638 443 387 323 226
165 152
AseI AT'TA_AT
Cuts at: 160 1032 2313 2514 5383 160
Size: 872 1281 201 2869 1606
Fragments arranged by size:
2869 1606 1281 872 201
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
AvaI C'yCGr_G
Cuts at: 1870 1943 2405 4154 1870
Size: 73 462 1749 4545
Fragments arranged by size:
4545 1749 462 73
BamHI G'GATC_C
Cuts at: 4159 4526 4159
Size: 367 6462
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2342 4150 5605 618
Size: 325 217 627 555 1808 1455 1842
Fragments arranged by size:
1842 1808 1455 627 555 325 217
BanII G_rGCy'C
Cuts at: 1391 2218 2909 3403 3495 4148 1391
Size: 827 691 494 92 653 4072
Fragments arranged by size:
4072 827 691 653 494 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3160 3787 928
Size: 971 1261 627 3970
Fragments arranged by size:
3970 1261 971 627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5020 5054 1903
Size: 34 3083 34 3678
Fragments arranged by size:
3678 3083 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4721 5931 4721
Size: 1210 5619
BclI T'GATC_A
Cuts at: 1548 3006 1548
Size: 1458 5371
BfrBI ATG'CAT
Cuts at: 3281 3281
Size: 6829
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3113 4001 5440
Size: 107 122 71 631 605 1441 888 1439
Cuts at: 5440 136
Size: 1525
Fragments arranged by size:
1525 1441 1439 888 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6824 6824
Size: 6829
BlpI GC'TnA_GC
Cuts at: 4246 4246
Size: 6829
Bme1580I G_kGCm'C
Cuts at: 1790 2367 3403 4890 6046 1790
Size: 577 1036 1487 1156 2573
Fragments arranged by size:
2573 1487 1156 1036 577
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6829
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2803 4560 5518
Size: 416 53 83 399 24 1386 1757 958
Cuts at: 5518 442
Size: 1753
Fragments arranged by size:
1757 1753 1386 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2433 2893 3261 5489 1239
Size: 1194 460 368 2228 2579
Fragments arranged by size:
2579 2228 1194 460 368
Bpu10I CC'TnA_GC
Cuts at: 2045 2045
Size: 6829
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4954 5819 6117 4954
Size: 865 298 5666
Fragments arranged by size:
5666 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5492 882
Size: 617 3993 2219
Fragments arranged by size:
3993 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2368 4620 493
Size: 711 1164 2252 2702
Fragments arranged by size:
2702 2252 1164 711
BsaBI GATnn'nnATC
Cuts at: 3005 6823 3005
Size: 3818 3011
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2201 4636 5018 5653
Size: 53 83 186 936 668 2435 382 635
Cuts at: 5653 275
Size: 1451
Fragments arranged by size:
2435 1451 936 668 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5262 5660 5934 6081 6492 5262
Size: 398 274 147 411 5599
Fragments arranged by size:
5599 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3234 3264 1178
Size: 30 165 30 1831 30 4743
Fragments arranged by size:
4743 1831 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6829
BseYI C'CCAG_C
Cuts at: 2142 2236 4027 6032 6528 2142
Size: 94 1791 2005 496 2443
Fragments arranged by size:
2443 2005 1791 496 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3158 1163
Size: 573 1422 4834
Fragments arranged by size:
4834 1422 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4279 5040 6068 1540
Size: 275 186 2278 761 1028 2301
Fragments arranged by size:
2301 2278 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2218 2367 2784 3495 3906 4890 4975 6046 2218
Size: 149 417 711 411 984 85 1071 3001
Fragments arranged by size:
3001 1071 984 711 417 411 149 85
BsmI GAATG_Cn'
Cuts at: 2106 4340 4433 2106
Size: 2234 93 4502
Fragments arranged by size:
4502 2234 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2218 2367 2784 2909 3403 3495 3906
Size: 399 428 149 417 125 494 92 411
Cuts at: 3906 4148 4890 4975 6046 1391
Size: 242 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 494 428 417 411 399
242 149 125 92 85
BspEI T'CCGG_A
Cuts at: 6492 6492
Size: 6829
BspHI T'CATG_A
Cuts at: 4718 6448 4718
Size: 1730 5099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3130 4184 844
Size: 2286 1054 3489
Fragments arranged by size:
3489 2286 1054
BsrBI CCG'CTC
Cuts at: 1394 1823 2818 4716 1394
Size: 429 995 1898 3507
Fragments arranged by size:
3507 1898 995 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3045 5324 5498 65
Size: 1183 1797 2279 174 1396
Fragments arranged by size:
2279 1797 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5473 5660 1700
Size: 110 3663 187 2869
Fragments arranged by size:
3663 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2670 96
Size: 2574 4255
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6829
BssSI C'ACGA_G
Cuts at: 2230 4889 5901 2230
Size: 2659 1012 3158
Fragments arranged by size:
3158 2659 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2371 794
Size: 1577 5252
BstBI TT'CG_AA
Cuts at: 5633 5633
Size: 6829
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6829
BstXI CCAn_nnnn'nTGG
Cuts at: 2883 2883
Size: 6829
BstYI r'GATC_y
Cuts at: 1972 4159 4526 4911 4928 6380 6466 6478 6824
Size: 2187 367 385 17 1452 86 12 346
Cuts at: 6824 1972
Size: 1977
Fragments arranged by size:
2187 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3111 3879 513
Size: 552 939 1107 768 3463
Fragments arranged by size:
3463 1107 939 768 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6090
Fragments arranged by size:
6090 683 56
BtsI GCAGTG_nn'
Cuts at: 2668 2918 3345 4345 5139 5159 2668
Size: 250 427 1000 794 20 4338
Fragments arranged by size:
4338 1000 794 427 250 20
ClaI AT'CG_AT
Cuts at: 1554 3150 1554
Size: 1596 5233
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6794
DraI TTT'AAA
Cuts at: 4133 4133
Size: 6829
DraIII CAC_nnn'GTG
Cuts at: 2371 2371
Size: 6829
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2257 5836 809
Size: 507 941 3579 1802
Fragments arranged by size:
3579 1802 941 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2712 4276 5165
Size: 54 1425 50 275 18 882 1564 889
Cuts at: 5165 6794 8
Size: 1629 43
Fragments arranged by size:
1629 1564 1425 889 882 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4276 1537
Size: 275 2464 4090
Fragments arranged by size:
4090 2464 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 3165 3240 4759 1353
Size: 1101 711 75 1519 3423
Fragments arranged by size:
3423 1519 1101 711 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3600 5416 5790 5936 387
Size: 1064 138 2011 1816 374 146 1280
Fragments arranged by size:
2011 1816 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2216 3493 2216
Size: 1277 5552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2433 2893 3261 3723 3842 4892 5489
Size: 393 801 460 368 462 119 1050 597
Cuts at: 5489 6276 6544 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1050 801 787 597 462 460 393
368 268 119
EcoO109I rG'GnC_Cy
Cuts at: 3400 3400
Size: 6829
EcoRI G'AATT_C
Cuts at: 2574 3815 4138 4516 2574
Size: 1241 323 378 4887
Fragments arranged by size:
4887 1241 378 323
FspI TGC'GCA
Cuts at: 5335 5335
Size: 6829
HaeII r_GCGC'y
Cuts at: 1678 5976 1678
Size: 4298 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 2205 2237 2277
Size: 32 135 32 569 32 32 32 40
Cuts at: 2277 2309 3327 3359 4027 4059 5470 5502 5544
Size: 32 1018 32 668 32 1411 32 42
Cuts at: 5544 5576 1373
Size: 32 2626
Fragments arranged by size:
2626 1411 1018 668 569 135 42 40
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3623 3742 4173 4419 669
Size: 988 281 1685 119 431 246 3079
Fragments arranged by size:
3079 1685 988 431 281 246 119
HindIII A'AGCT_T
Cuts at: 748 2589 748
Size: 1841 4988
HpaI GTT'AAC
Cuts at: 3742 4419 3742
Size: 677 6152
KpnI G_GTAC'C
Cuts at: 4154 4154
Size: 6829
MfeI C'AATT_G
Cuts at: 2280 4428 2280
Size: 2148 4681
MluI A'CGCG_T
Cuts at: 6705 6705
Size: 6829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3864 5943 6127 1305
Size: 2559 2079 184 2007
Fragments arranged by size:
2559 2079 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2714 10
Size: 54 1425 343 882 4125
Fragments arranged by size:
4125 1425 882 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2334 2549 3506 3899 4787 5146
Size: 648 843 325 215 957 393 888 359
Cuts at: 5146 5305 518
Size: 159 2042
Fragments arranged by size:
2042 957 888 843 648 393 359 325
215 159
MspA1I CmG'CkG
Cuts at: 1606 2142 3696 4031 4245 4922 6070 6315 1606
Size: 536 1554 335 214 677 1148 245 2120
Fragments arranged by size:
2120 1554 1148 677 536 335 245 214
NcoI C'CATG_G
Cuts at: 513 1065 2004 3111 513
Size: 552 939 1107 4231
Fragments arranged by size:
4231 1107 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6829
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6829
NotI GC'GGCC_GC
Cuts at: 4276 4276
Size: 6829
NsiI A_TGCA'T
Cuts at: 3283 3283
Size: 6829
NspI r_CATG'y
Cuts at: 1132 1229 4187 5732 1132
Size: 97 2958 1545 2229
Fragments arranged by size:
2958 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6829
PciI A'CATG_T
Cuts at: 5728 5728
Size: 6829
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6829
PfoI T'CCnGG_A
Cuts at: 2939 3238 3331 2939
Size: 299 93 6437
Fragments arranged by size:
6437 299 93
PmeI GTTT'AAAC
Cuts at: 4133 4133
Size: 6829
PmlI CAC'GTG
Cuts at: 2368 2368
Size: 6829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4898 4930 6411 6443 4898
Size: 32 1481 32 5284
Fragments arranged by size:
5284 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6829
PsiI TTA'TAA
Cuts at: 4399 4399
Size: 6829
PspOMI G'GGCC_C
Cuts at: 3399 3399
Size: 6829
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2663 2695 2663
Size: 32 6797
PstI C_TGCA'G
Cuts at: 830 2925 4181 830
Size: 2095 1256 3478
Fragments arranged by size:
3478 2095 1256
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6829
PvuII CAG'CTG
Cuts at: 2142 3696 2142
Size: 1554 5275
SacI G_AGCT'C
Cuts at: 2218 3495 2218
Size: 1277 5552
SalI G'TCGA_C
Cuts at: 1936 4171 1936
Size: 2235 4594
SbfI CC_TGCA'GG
Cuts at: 4181 4181
Size: 6829
ScaI AGT'ACT
Cuts at: 5077 6789 5077
Size: 1712 5117
SexAI A'CCwGG_T
Cuts at: 3414 3414
Size: 6829
SfcI C'TryA_G
Cuts at: 826 1045 2634 2921 4177 5312 5993 6184 826
Size: 219 1589 287 1256 1135 681 191 1471
Fragments arranged by size:
1589 1471 1256 1135 681 287 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3113 3113
Size: 6829
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6829
SmaI CCC'GGG
Cuts at: 1872 4156 1872
Size: 2284 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4933 5834 6096 820
Size: 197 926 2990 901 262 1553
Fragments arranged by size:
2990 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4620 493
Size: 4127 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6829
SphI G_CATG'C
Cuts at: 1132 4187 1132
Size: 3055 3774
SspI AAT'ATT
Cuts at: 5 52 4753 5
Size: 47 4701 2081
Fragments arranged by size:
4701 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6829
StyI C'CwwG_G
Cuts at: 513 1065 2004 2656 3111 4268 513
Size: 552 939 652 455 1157 3074
Fragments arranged by size:
3074 1157 939 652 552 455
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2563 4856 4873 5026 5211 5656 325
Size: 1255 983 2293 17 153 185 445 1498
Fragments arranged by size:
2293 1498 1255 983 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2121 2670 3948
Size: 274 80 33 51 1443 144 549 1278
Cuts at: 3948 5075 6787 96
Size: 1127 1712 138
Fragments arranged by size:
1712 1443 1278 1127 549 274 144 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3234 3266 6789 6821 3234
Size: 32 3523 32 3242
Fragments arranged by size:
3523 3242 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6829
XbaI T'CTAG_A
Cuts at: 4165 4165
Size: 6829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2336 2503 3118 3139 2336
Size: 167 615 21 6026
Fragments arranged by size:
6026 615 167 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6829
XmaI C'CCGG_G
Cuts at: 1870 4154 1870
Size: 2284 4545
XmnI GAAnn'nnTTC
Cuts at: 4958 4958
Size: 6829
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4637 276
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI
PspXI RsrII SacII SanDI SapI SfoI SrfI SwaI