Restriction map for
pFN21AE5750
(Circular) (Six-base) MAPSORT of: pFN21AE5750.seq Check: 7440 from: 1 to: 6781
pFN21AE5750 6781 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2408 2408
Size: 6781
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4591 278
Size: 53 83 186 936 3055 2468
Fragments arranged by size:
3055 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4124 1937
Size: 2187 4594
Acc65I G'GTAC_C
Cuts at: 4102 4102
Size: 6781
AclI AA'CG_TT
Cuts at: 1563 4908 5281 1563
Size: 3345 373 3063
Fragments arranged by size:
3345 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2241 3068 3578 4844 6228 6496 1632
Size: 609 827 510 1266 1384 268 1917
Fragments arranged by size:
1917 1384 1266 827 609 510 268
AfeI AGC'GCT
Cuts at: 3063 3599 3063
Size: 536 6245
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6584
AflIII A'CryG_T
Cuts at: 1203 5680 6657 1203
Size: 4477 977 1327
Fragments arranged by size:
4477 1327 977
AgeI A'CCGG_T
Cuts at: 5612 5612
Size: 6781
AhdI GACnn_n'nnGTC
Cuts at: 5510 5510
Size: 6781
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6781
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6781
AlwNI CAG_nnn'CTG
Cuts at: 3567 3956 4524 6096 3567
Size: 389 568 1572 4252
Fragments arranged by size:
4252 1572 568 389
ApaI G_GGCC'C
Cuts at: 3010 3947 3010
Size: 937 5844
ApaLI G'TGCA_C
Cuts at: 4838 5994 4838
Size: 1156 5625
ApoI r'AATT_y
Cuts at: 1493 4090 4316 4468 6577 1493
Size: 2597 226 152 2109 1697
Fragments arranged by size:
2597 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5335 160
Size: 872 4303 1606
Fragments arranged by size:
4303 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2469 3001 3257 4106 1870
Size: 73 85 441 532 256 849 4545
Fragments arranged by size:
4545 849 532 441 256 85 73
BamHI G'GATC_C
Cuts at: 2551 4111 4478 2551
Size: 1560 367 4854
Fragments arranged by size:
4854 1560 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2682 2887 3113 3415
Size: 325 217 627 297 598 205 226 302
Cuts at: 3415 3527 4102 5557 618
Size: 112 575 1455 1842
Fragments arranged by size:
1842 1455 627 598 575 325 302 297
226 217 205 112
BanII G_rGCy'C
Cuts at: 1391 2029 2235 2477 3010 3673 3835 3947 3999
Size: 638 206 242 533 663 162 112 52
Cuts at: 3999 4100 1391
Size: 101 4072
Fragments arranged by size:
4072 663 638 533 242 206 162 112
101 52
BbeI G_GCGC'C
Cuts at: 2088 2088
Size: 6781
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5810
BbvCI CC'TCA_GC
Cuts at: 2429 3767 2429
Size: 1338 5443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4972 5006 1903
Size: 34 3035 34 3678
Fragments arranged by size:
3678 3035 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2270 4673 5883 2270
Size: 2403 1210 3168
Fragments arranged by size:
3168 2403 1210
BclI T'GATC_A
Cuts at: 1548 2421 1548
Size: 873 5908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2216 3803 5392
Size: 107 122 71 631 605 544 1587 1589
Cuts at: 5392 136
Size: 1525
Fragments arranged by size:
1589 1587 1525 631 605 544 122 107
71
BglII A'GATC_T
Cuts at: 2499 6776 2499
Size: 4277 2504
BlpI GC'TnA_GC
Cuts at: 4198 4198
Size: 6781
Bme1580I G_kGCm'C
Cuts at: 1790 3010 3116 3490 3947 4842 5998 1790
Size: 1220 106 374 457 895 1156 2573
Fragments arranged by size:
2573 1220 1156 895 457 374 106
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6781
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2143 3004 3477
Size: 416 53 83 399 24 726 861 473
Cuts at: 3477 3526 4512 5470 442
Size: 49 986 958 1753
Fragments arranged by size:
1753 986 958 861 726 473 416 399
83 53 49 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 6781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2192 2234 2267 2804 3078 3230 3645
Size: 793 160 42 33 537 274 152 415
Cuts at: 3645 5441 1239
Size: 1796 2579
Fragments arranged by size:
2579 1796 793 537 415 274 160 152
42 33
Bpu10I CC'TnA_GC
Cuts at: 2429 3767 2429
Size: 1338 5443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2261 4906 5771 6069 2261
Size: 2645 865 298 2973
Fragments arranged by size:
2973 2645 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2188 5444 882
Size: 617 689 3256 2219
Fragments arranged by size:
3256 2219 689 617
BsaAI yAC'GTr
Cuts at: 493 1204 2734 4572 493
Size: 711 1530 1838 2702
Fragments arranged by size:
2702 1838 1530 711
BsaBI GATnn'nnATC
Cuts at: 6775 6775
Size: 6781
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 4588 4970 5605
Size: 53 83 186 936 552 2503 382 635
Cuts at: 5605 275
Size: 1451
Fragments arranged by size:
2503 1451 936 635 552 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5214 5612 5886 6033 6444 5214
Size: 398 274 147 411 5551
Fragments arranged by size:
5551 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2697 2727 3203 3233 1178
Size: 30 165 30 1294 30 476 30 4726
Fragments arranged by size:
4726 1294 476 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 2945 3109 3755 1186
Size: 1003 756 164 646 4212
Fragments arranged by size:
4212 1003 756 646 164
BseYI C'CCAG_C
Cuts at: 3186 3250 3392 3489 3666 3852 3998 5984 6480
Size: 64 142 97 177 186 146 1986 496
Cuts at: 6480 3186
Size: 3487
Fragments arranged by size:
3487 1986 496 186 177 146 142 97
64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2380 3363 3746 3898 1163
Size: 573 644 983 383 152 4046
Fragments arranged by size:
4046 983 644 573 383 152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4231 4992 6020 1540
Size: 275 186 2230 761 1028 2301
Fragments arranged by size:
2301 2230 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2029 2235 3661 4842 4927 5998 2029
Size: 206 1426 1181 85 1071 2812
Fragments arranged by size:
2812 1426 1181 1071 206 85
BsmI GAATG_Cn'
Cuts at: 2443 3583 4292 4385 2443
Size: 1140 709 93 4839
Fragments arranged by size:
4839 1140 709 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2717 2717
Size: 6781
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2235 2477 3010 3116 3490 3661
Size: 399 239 206 242 533 106 374 171
Cuts at: 3661 3673 3835 3947 3999 4100 4842 4927 5998
Size: 12 162 112 52 101 742 85 1071
Cuts at: 5998 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 533 399 374 242 239
206 171 162 112 106 101 85 52
12
BspEI T'CCGG_A
Cuts at: 6444 6444
Size: 6781
BspHI T'CATG_A
Cuts at: 3991 4670 6400 3991
Size: 679 1730 4372
Fragments arranged by size:
4372 1730 679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2408 3507 4136 844
Size: 1564 1099 629 3489
Fragments arranged by size:
3489 1564 1099 629
BsrBI CCG'CTC
Cuts at: 1394 1823 2800 4668 1394
Size: 429 977 1868 3507
Fragments arranged by size:
3507 1868 977 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2341 5276 5450 65
Size: 1183 1093 2935 174 1396
Fragments arranged by size:
2935 1396 1183 1093 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2179 2777 2893 3052 3797 5425 5612
Size: 110 369 598 116 159 745 1628 187
Cuts at: 5612 1700
Size: 2869
Fragments arranged by size:
2869 1628 745 598 369 187 159 116
110
BsrGI T'GTAC_A
Cuts at: 96 2646 96
Size: 2550 4231
BssHII G'CGCG_C
Cuts at: 1925 2618 2824 1925
Size: 693 206 5882
Fragments arranged by size:
5882 693 206
BssSI C'ACGA_G
Cuts at: 2495 4841 5853 2495
Size: 2346 1012 3423
Fragments arranged by size:
3423 2346 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3415 3567 794
Size: 2621 152 4008
Fragments arranged by size:
4008 2621 152
BstBI TT'CG_AA
Cuts at: 5585 5585
Size: 6781
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6781
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 3940 2734
Size: 1206 5575
BstYI r'GATC_y
Cuts at: 1972 2499 2551 4067 4111 4478 4863 4880 6332
Size: 527 52 1516 44 367 385 17 1452
Cuts at: 6332 6418 6430 6776 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1516 1452 527 385 367 346 86
52 44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3019 3078 3622 3933 513
Size: 552 939 1015 59 544 311 3361
Fragments arranged by size:
3361 1015 939 552 544 311 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3859 487
Size: 683 56 2633 3409
Fragments arranged by size:
3409 2633 683 56
BtsI GCAGTG_nn'
Cuts at: 3181 3224 3380 3886 4297 5091 5111 3181
Size: 43 156 506 411 794 20 4851
Fragments arranged by size:
4851 794 506 411 156 43 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6781
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3611 3646 529
Size: 35 3047 35 3664
Fragments arranged by size:
3664 3047 35 35
DraI TTT'AAA
Cuts at: 4085 4085
Size: 6781
DraIII CAC_nnn'GTG
Cuts at: 3164 3415 3534 3164
Size: 251 119 6411
Fragments arranged by size:
6411 251 119
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5788 809
Size: 507 4472 1802
Fragments arranged by size:
4472 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2072 2462 2542
Size: 54 1425 50 275 18 242 390 80
Cuts at: 2542 2950 3310 3445 3756 4228 5117 6746 8
Size: 408 360 135 311 472 889 1629 43
Fragments arranged by size:
1629 1425 889 472 408 390 360 311
275 242 135 80 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4228 1537
Size: 275 2416 4090
Fragments arranged by size:
4090 2416 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4711 1353
Size: 3358 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5368 5742 5888 387
Size: 1064 138 3779 374 146 1280
Fragments arranged by size:
3779 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2027 2233 2027
Size: 206 6575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2192 2234 2241 2267 2804 3068
Size: 393 400 160 42 7 26 537 264
Cuts at: 3068 3078 3230 3578 3645 4844 5441 6228 6496
Size: 10 152 348 67 1199 597 787 268
Cuts at: 6496 1239
Size: 1524
Fragments arranged by size:
1524 1199 787 597 537 400 393 348
268 264 160 152 67 42 26 10
7
EcoNI CCTnn'n_nnAGG
Cuts at: 3166 3166
Size: 6781
EcoO109I rG'GnC_Cy
Cuts at: 2016 2034 2137 2625 3006 3236 3868 3944 2016
Size: 18 103 488 381 230 632 76 4853
Fragments arranged by size:
4853 632 488 381 230 103 76 18
EcoRI G'AATT_C
Cuts at: 4090 4468 4090
Size: 378 6403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3598 3712 3598
Size: 114 6667
FseI GG_CCGG'CC
Cuts at: 3801 3801
Size: 6781
FspI TGC'GCA
Cuts at: 5287 5287
Size: 6781
HaeII r_GCGC'y
Cuts at: 1678 2088 2774 2853 2900 3065 3568 3601 5928
Size: 410 686 79 47 165 503 33 2327
Cuts at: 5928 1678
Size: 2531
Fragments arranged by size:
2531 2327 686 503 410 165 79 47
33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4059 4091 5422 5454 5496
Size: 32 135 32 2487 32 1331 32 42
Cuts at: 5496 5528 1373
Size: 32 2626
Fragments arranged by size:
2626 2487 1331 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4125 4371 669
Size: 988 281 2187 246 3079
Fragments arranged by size:
3079 2187 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6781
HpaI GTT'AAC
Cuts at: 4371 4371
Size: 6781
KasI G'GCGC_C
Cuts at: 2084 2084
Size: 6781
KpnI G_GTAC'C
Cuts at: 4106 4106
Size: 6781
MfeI C'AATT_G
Cuts at: 4380 4380
Size: 6781
MluI A'CGCG_T
Cuts at: 6657 6657
Size: 6781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5895 6079 1305
Size: 4590 184 2007
Fragments arranged by size:
4590 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2074 2464 2544 2952 3447
Size: 54 1425 343 242 390 80 408 495
Cuts at: 3447 3758 10
Size: 311 3033
Fragments arranged by size:
3033 1425 495 408 390 343 311 242
80 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2765 3441 4739 5098 5257 518
Size: 648 1599 676 1298 359 159 2042
Fragments arranged by size:
2042 1599 1298 676 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2518 2821 3493 3852 4197 4874 6022 6267
Size: 912 303 672 359 345 677 1148 245
Cuts at: 6267 1606
Size: 2120
Fragments arranged by size:
2120 1148 912 677 672 359 345 303
245
NaeI GCC'GGC
Cuts at: 2895 3054 3799 2895
Size: 159 745 5877
Fragments arranged by size:
5877 745 159
NarI GG'CG_CC
Cuts at: 2085 2085
Size: 6781
NcoI C'CATG_G
Cuts at: 513 1065 2004 3078 3933 513
Size: 552 939 1074 855 3361
Fragments arranged by size:
3361 1074 939 855 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6781
NgoMIV G'CCGG_C
Cuts at: 2893 3052 3797 2893
Size: 159 745 5877
Fragments arranged by size:
5877 745 159
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6781
NotI GC'GGCC_GC
Cuts at: 4228 4228
Size: 6781
NspI r_CATG'y
Cuts at: 1132 1229 3201 4139 5684 1132
Size: 97 1972 938 1545 2229
Fragments arranged by size:
2229 1972 1545 938 97
PasI CC'CwG_GG
Cuts at: 1348 2111 3791 3916 1348
Size: 763 1680 125 4213
Fragments arranged by size:
4213 1680 763 125
PciI A'CATG_T
Cuts at: 5680 5680
Size: 6781
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 3079 3698 3916 1108
Size: 902 1069 619 218 3973
Fragments arranged by size:
3973 1069 902 619 218
PfoI T'CCnGG_A
Cuts at: 2139 2715 3585 2139
Size: 576 870 5335
Fragments arranged by size:
5335 870 576
PmeI GTTT'AAAC
Cuts at: 4085 4085
Size: 6781
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4850 4882 6363 6395 4850
Size: 32 1481 32 5236
Fragments arranged by size:
5236 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2034 2137 2625 3868 2034
Size: 103 488 1243 4947
Fragments arranged by size:
4947 1243 488 103
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6781
PsiI TTA'TAA
Cuts at: 4351 4351
Size: 6781
PspOMI G'GGCC_C
Cuts at: 3006 3943 3006
Size: 937 5844
PstI C_TGCA'G
Cuts at: 830 3375 3572 3843 3863 4133 830
Size: 2545 197 271 20 270 3478
Fragments arranged by size:
3478 2545 271 270 197 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6781
PvuII CAG'CTG
Cuts at: 2518 2821 3493 3852 2518
Size: 303 672 359 5447
Fragments arranged by size:
5447 672 359 303
SacI G_AGCT'C
Cuts at: 2029 2235 2029
Size: 206 6575
SalI G'TCGA_C
Cuts at: 1936 4123 1936
Size: 2187 4594
SanDI GG'GwC_CC
Cuts at: 2034 3868 2034
Size: 1834 4947
SbfI CC_TGCA'GG
Cuts at: 4133 4133
Size: 6781
ScaI AGT'ACT
Cuts at: 5029 6741 5029
Size: 1712 5069
SfcI C'TryA_G
Cuts at: 826 1045 3371 3568 3839 3859 4129 5264 5945
Size: 219 2326 197 271 20 270 1135 681
Cuts at: 5945 6136 826
Size: 191 1471
Fragments arranged by size:
2326 1471 1135 681 271 270 219 197
191 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 3803 3803
Size: 6781
SfoI GGC'GCC
Cuts at: 2086 2086
Size: 6781
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
SgrAI Cr'CCGG_yG
Cuts at: 1700 2893 1700
Size: 1193 5588
SmaI CCC'GGG
Cuts at: 1872 2471 3003 4108 1872
Size: 599 532 1105 4545
Fragments arranged by size:
4545 1105 599 532
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 4885 5786 6048 820
Size: 197 926 297 2645 901 262 1553
Fragments arranged by size:
2645 1553 926 901 297 262 197
SnaBI TAC'GTA
Cuts at: 493 4572 493
Size: 4079 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6781
SphI G_CATG'C
Cuts at: 1132 4139 1132
Size: 3007 3774
SspI AAT'ATT
Cuts at: 5 52 4705 5
Size: 47 4653 2081
Fragments arranged by size:
4653 2081 47
StuI AGG'CCT
Cuts at: 1517 3263 1517
Size: 1746 5035
StyI C'CwwG_G
Cuts at: 513 1065 2004 2353 2478 3078 3471 3933 4220
Size: 552 939 349 125 600 393 462 287
Cuts at: 4220 513
Size: 3074
Fragments arranged by size:
3074 939 600 552 462 393 349 287
125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2810 3071 3264 3545 4808 4825 4978
Size: 1255 1230 261 193 281 1263 17 153
Cuts at: 4978 5163 5608 325
Size: 185 445 1498
Fragments arranged by size:
1498 1263 1255 1230 445 281 261 193
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2646 5027 6739
Size: 274 80 33 51 1443 669 2381 1712
Cuts at: 6739 96
Size: 138
Fragments arranged by size:
2381 1712 1443 669 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2871 2903 3203 3235 3344 3376 3860 3892 6741
Size: 32 300 32 109 32 484 32 2849
Cuts at: 6741 6773 2871
Size: 32 2879
Fragments arranged by size:
2879 2849 484 300 109 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6781
XbaI T'CTAG_A
Cuts at: 4117 4117
Size: 6781
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2552 3490 2552
Size: 938 5843
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6781
XmaI C'CCGG_G
Cuts at: 1870 2469 3001 4106 1870
Size: 599 532 1105 4545
Fragments arranged by size:
4545 1105 599 532
XmnI GAAnn'nnTTC
Cuts at: 2912 3581 4910 2912
Size: 669 1329 4783
Fragments arranged by size:
4783 1329 669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4589 276
Size: 53 83 186 936 3055 2468
Fragments arranged by size:
3055 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SapI SexAI SrfI SwaI