Restriction map for
pFN21AE5749
(Circular) (Six-base) MAPSORT of: pFN21AE5749.seq Check: 7013 from: 1 to: 6724
pFN21AE5749 6724 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3493 3493
Size: 6724
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4534 278
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3353 4067 1937
Size: 1416 714 4594
Fragments arranged by size:
4594 1416 714
Acc65I G'GTAC_C
Cuts at: 4045 4045
Size: 6724
AclI AA'CG_TT
Cuts at: 1563 4851 5224 1563
Size: 3288 373 3063
Fragments arranged by size:
3288 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3345 3557 4787 6171 6439 1632
Size: 1713 212 1230 1384 268 1917
Fragments arranged by size:
1917 1713 1384 1230 268 212
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6527
AflIII A'CryG_T
Cuts at: 1203 3573 5623 6600 1203
Size: 2370 2050 977 1327
Fragments arranged by size:
2370 2050 1327 977
AgeI A'CCGG_T
Cuts at: 5555 5555
Size: 6724
AhdI GACnn_n'nnGTC
Cuts at: 2048 3321 5453 2048
Size: 1273 2132 3319
Fragments arranged by size:
3319 2132 1273
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6724
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2636 1184
Size: 1452 5272
AlwNI CAG_nnn'CTG
Cuts at: 3368 3608 3833 4467 6039 3368
Size: 240 225 634 1572 4053
Fragments arranged by size:
4053 1572 634 240 225
ApaI G_GGCC'C
Cuts at: 3711 3962 3711
Size: 251 6473
ApaLI G'TGCA_C
Cuts at: 4781 5937 4781
Size: 1156 5568
ApoI r'AATT_y
Cuts at: 1493 2157 4033 4259 4411 6520 1493
Size: 664 1876 226 152 2109 1697
Fragments arranged by size:
2109 1876 1697 664 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5278 160
Size: 872 4246 1606
Fragments arranged by size:
4246 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
AvaI C'yCGr_G
Cuts at: 1870 1943 2241 2280 2435 2840 3054 3429 4049
Size: 73 298 39 155 405 214 375 620
Cuts at: 4049 1870
Size: 4545
Fragments arranged by size:
4545 620 405 375 298 214 155 73
39
BamHI G'GATC_C
Cuts at: 3418 3541 4054 4421 3418
Size: 123 513 367 5721
Fragments arranged by size:
5721 513 367 123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2747 3050 3636 4045 5500
Size: 325 217 627 960 303 586 409 1455
Cuts at: 5500 618
Size: 1842
Fragments arranged by size:
1842 1455 960 627 586 409 325 303
217
BanII G_rGCy'C
Cuts at: 1391 2538 3248 3393 3591 3711 3962 3981 4043
Size: 1147 710 145 198 120 251 19 62
Cuts at: 4043 1391
Size: 4072
Fragments arranged by size:
4072 1147 710 251 198 145 120 62
19
BbeI G_GCGC'C
Cuts at: 3640 3640
Size: 6724
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2115 3675 3915 928
Size: 971 216 1560 240 3737
Fragments arranged by size:
3737 1560 971 240 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4915 4949 1903
Size: 34 2978 34 3678
Fragments arranged by size:
3678 2978 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4616 5826 4616
Size: 1210 5514
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6724
BfrBI ATG'CAT
Cuts at: 2596 2596
Size: 6724
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5335 136
Size: 107 122 71 631 605 3663 1525
Fragments arranged by size:
3663 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6719 6719
Size: 6724
BlpI GC'TnA_GC
Cuts at: 4141 4141
Size: 6724
Bme1580I G_kGCm'C
Cuts at: 1790 2436 2901 3711 3962 4785 5941 1790
Size: 646 465 810 251 823 1156 2573
Fragments arranged by size:
2573 1156 823 810 646 465 251
BmgBI CAC'GTC
Cuts at: 1318 3576 1318
Size: 2258 4466
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3468 4455 5413
Size: 416 53 83 399 24 2051 987 958
Cuts at: 5413 442
Size: 1753
Fragments arranged by size:
2051 1753 987 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6724
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 6724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2172 2381 3107 5384 1239
Size: 933 209 726 2277 2579
Fragments arranged by size:
2579 2277 933 726 209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2891 4849 5714 6012 2891
Size: 1958 865 298 3603
Fragments arranged by size:
3603 1958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2801 3810 5387 882
Size: 617 1302 1009 1577 2219
Fragments arranged by size:
2219 1577 1302 1009 617
BsaAI yAC'GTr
Cuts at: 493 1204 4515 493
Size: 711 3311 2702
Fragments arranged by size:
3311 2702 711
BsaBI GATnn'nnATC
Cuts at: 6718 6718
Size: 6724
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3637 4531 4913 5548
Size: 53 83 186 936 2104 894 382 635
Cuts at: 5548 275
Size: 1451
Fragments arranged by size:
2104 1451 936 894 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5157 5555 5829 5976 6387 5157
Size: 398 274 147 411 5494
Fragments arranged by size:
5494 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6499
Fragments arranged by size:
6499 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2711 3035 3238 3241 3244 3401 1186
Size: 1525 324 203 3 3 157 4509
Fragments arranged by size:
4509 1525 324 203 157 3 3
BseYI C'CCAG_C
Cuts at: 2584 3172 3338 3401 3622 5927 6423 2584
Size: 588 166 63 221 2305 496 2885
Fragments arranged by size:
2885 2305 588 496 221 166 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2797 1163
Size: 573 1061 5090
Fragments arranged by size:
5090 1061 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3455 4174 4935 5963 1540
Size: 275 186 1454 719 761 1028 2301
Fragments arranged by size:
2301 1454 1028 761 719 275 186
BsiHKAI G_wGCw'C
Cuts at: 2538 3248 3981 4785 4870 5941 2538
Size: 710 733 804 85 1071 3321
Fragments arranged by size:
3321 1071 804 733 710 85
BsmI GAATG_Cn'
Cuts at: 4235 4328 4235
Size: 93 6631
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2436 2538 2901 3248 3393 3591 3711
Size: 399 646 102 363 347 145 198 120
Cuts at: 3711 3962 3981 4043 4785 4870 5941 1391
Size: 251 19 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 646 399 363 347 251
198 145 120 102 85 62 19
BspEI T'CCGG_A
Cuts at: 6387 6387
Size: 6724
BspHI T'CATG_A
Cuts at: 4613 6343 4613
Size: 1730 4994
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2819 3114 3493 4079 844
Size: 1975 295 379 586 3489
Fragments arranged by size:
3489 1975 586 379 295
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 4611 1394
Size: 429 1822 966 3507
Fragments arranged by size:
3507 1822 966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2644 3076 5219 5393 65
Size: 1183 1396 432 2143 174 1396
Fragments arranged by size:
2143 1396 1396 1183 432 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5368 5555 1700
Size: 110 3558 187 2869
Fragments arranged by size:
3558 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6724
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6724
BssSI C'ACGA_G
Cuts at: 3852 4784 5796 3852
Size: 932 1012 4780
Fragments arranged by size:
4780 1012 932
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6724
BstBI TT'CG_AA
Cuts at: 5528 5528
Size: 6724
BstEII G'GTnAC_C
Cuts at: 1184 2419 2782 1184
Size: 1235 363 5126
Fragments arranged by size:
5126 1235 363
BstXI CCAn_nnnn'nTGG
Cuts at: 2797 2908 2797
Size: 111 6613
BstYI r'GATC_y
Cuts at: 1972 2440 3418 3541 4054 4421 4806 4823 6275
Size: 468 978 123 513 367 385 17 1452
Cuts at: 6275 6361 6373 6719 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 978 513 468 385 367 346
123 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3954 3954
Size: 6724
BtgI C'CryG_G
Cuts at: 513 1065 2004 2424 3298 3304 3784 513
Size: 552 939 420 874 6 480 3453
Fragments arranged by size:
3453 939 874 552 480 420 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5985
Fragments arranged by size:
5985 683 56
BtsI GCAGTG_nn'
Cuts at: 4240 5034 5054 4240
Size: 794 20 5910
Fragments arranged by size:
5910 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6724
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6689
DraI TTT'AAA
Cuts at: 4028 4028
Size: 6724
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3677 3753 5731 809
Size: 507 2361 76 1978 1802
Fragments arranged by size:
2361 1978 1802 507 76
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2372 3301 3307
Size: 54 1425 50 275 18 542 929 6
Cuts at: 3307 3452 4171 5060 6689 8
Size: 145 719 889 1629 43
Fragments arranged by size:
1629 1425 929 889 719 542 275 145
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3452 4171 1537
Size: 275 1640 719 4090
Fragments arranged by size:
4090 1640 719 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2543 2799 3374 4654 1353
Size: 1190 256 575 1280 3423
Fragments arranged by size:
3423 1280 1190 575 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3973 5311 5685 5831 387
Size: 1064 138 2384 1338 374 146 1280
Fragments arranged by size:
2384 1338 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2536 3246 3979 2536
Size: 710 733 5281
Fragments arranged by size:
5281 733 710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2381 3107 3345 3557 4787 5384
Size: 393 540 209 726 238 212 1230 597
Cuts at: 5384 6171 6439 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1230 787 726 597 540 393 268
238 212 209
EcoNI CCTnn'n_nnAGG
Cuts at: 3110 3110
Size: 6724
EcoO109I rG'GnC_Cy
Cuts at: 2073 2716 3476 3958 3959 2073
Size: 643 760 482 1 4838
Fragments arranged by size:
4838 760 643 482 1
EcoRI G'AATT_C
Cuts at: 4033 4411 4033
Size: 378 6346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3932 3932
Size: 6724
FspI TGC'GCA
Cuts at: 2612 5230 2612
Size: 2618 4106
HaeII r_GCGC'y
Cuts at: 1678 3640 5871 1678
Size: 1962 2231 2531
Fragments arranged by size:
2531 2231 1962
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2066 3048 3080 3230
Size: 32 135 32 462 32 982 32 150
Cuts at: 3230 3262 3307 3339 3356 3388 3833 3865 5365
Size: 32 45 32 17 32 445 32 1500
Cuts at: 5365 5397 5439 5471 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1500 982 462 445 150 135 45
42 32 32 32 32 32 32 32
32 32 32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 2550 4068 4314 669
Size: 988 281 612 1518 246 3079
Fragments arranged by size:
3079 1518 988 612 281 246
HindIII A'AGCT_T
Cuts at: 748 3947 748
Size: 3199 3525
HpaI GTT'AAC
Cuts at: 4314 4314
Size: 6724
KasI G'GCGC_C
Cuts at: 3636 3636
Size: 6724
KpnI G_GTAC'C
Cuts at: 4049 4049
Size: 6724
MfeI C'AATT_G
Cuts at: 2733 4323 2733
Size: 1590 5134
MluI A'CGCG_T
Cuts at: 6600 6600
Size: 6724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 5838 6022 1305
Size: 786 3747 184 2007
Fragments arranged by size:
3747 2007 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4902
Fragments arranged by size:
4902 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 4682 5041 5200 518
Size: 648 1263 2253 359 159 2042
Fragments arranged by size:
2253 2042 1263 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2192 3207 4140 4817 5965 6210 1606
Size: 586 1015 933 677 1148 245 2120
Fragments arranged by size:
2120 1148 1015 933 677 586 245
NarI GG'CG_CC
Cuts at: 3637 3637
Size: 6724
NcoI C'CATG_G
Cuts at: 513 1065 2004 2424 513
Size: 552 939 420 4813
Fragments arranged by size:
4813 939 552 420
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6724
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6724
NotI GC'GGCC_GC
Cuts at: 4171 4171
Size: 6724
NsiI A_TGCA'T
Cuts at: 2598 2598
Size: 6724
NspI r_CATG'y
Cuts at: 1132 1229 4082 5627 1132
Size: 97 2853 1545 2229
Fragments arranged by size:
2853 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2494 3015 1348
Size: 1146 521 5057
Fragments arranged by size:
5057 1146 521
PciI A'CATG_T
Cuts at: 5623 5623
Size: 6724
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6724
PfoI T'CCnGG_A
Cuts at: 2946 2946
Size: 6724
PmeI GTTT'AAAC
Cuts at: 4028 4028
Size: 6724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4793 4825 6306 6338 4793
Size: 32 1481 32 5179
Fragments arranged by size:
5179 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2716 3476 2716
Size: 760 5964
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6724
PsiI TTA'TAA
Cuts at: 4294 4294
Size: 6724
PspOMI G'GGCC_C
Cuts at: 3707 3958 3707
Size: 251 6473
PstI C_TGCA'G
Cuts at: 830 3111 4076 830
Size: 2281 965 3478
Fragments arranged by size:
3478 2281 965
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6724
PvuII CAG'CTG
Cuts at: 2192 2192
Size: 6724
SacI G_AGCT'C
Cuts at: 2538 3248 3981 2538
Size: 710 733 5281
Fragments arranged by size:
5281 733 710
SalI G'TCGA_C
Cuts at: 1936 4066 1936
Size: 2130 4594
SanDI GG'GwC_CC
Cuts at: 3476 3476
Size: 6724
SapI GCTCTTCn'nnn_
Cuts at: 2543 2543
Size: 6724
SbfI CC_TGCA'GG
Cuts at: 3111 4076 3111
Size: 965 5759
ScaI AGT'ACT
Cuts at: 4972 6684 4972
Size: 1712 5012
SexAI A'CCwGG_T
Cuts at: 2907 2907
Size: 6724
SfcI C'TryA_G
Cuts at: 826 1045 3107 3354 3559 4072 5207 5888 6079
Size: 219 2062 247 205 513 1135 681 191
Cuts at: 6079 826
Size: 1471
Fragments arranged by size:
2062 1471 1135 681 513 247 219 205
191
SfoI GGC'GCC
Cuts at: 3638 3638
Size: 6724
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6724
SmaI CCC'GGG
Cuts at: 1872 2282 4051 1872
Size: 410 1769 4545
Fragments arranged by size:
4545 1769 410
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2241 2870 4828 5729 5991 820
Size: 197 926 298 629 1958 901 262 1553
Fragments arranged by size:
1958 1553 926 901 629 298 262 197
SnaBI TAC'GTA
Cuts at: 493 4515 493
Size: 4022 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6724
SphI G_CATG'C
Cuts at: 1132 4082 1132
Size: 2950 3774
SspI AAT'ATT
Cuts at: 5 52 4648 5
Size: 47 4596 2081
Fragments arranged by size:
4596 2081 47
StuI AGG'CCT
Cuts at: 1517 3693 1517
Size: 2176 4548
StyI C'CwwG_G
Cuts at: 513 1065 2004 2424 3990 4163 513
Size: 552 939 420 1566 173 3074
Fragments arranged by size:
3074 1566 939 552 420 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2576 2789 4751 4768 4921 5106 5551
Size: 1255 996 213 1962 17 153 185 445
Cuts at: 5551 325
Size: 1498
Fragments arranged by size:
1962 1498 1255 996 445 213 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4970 6682 96
Size: 274 80 33 51 1443 2993 1712 138
Fragments arranged by size:
2993 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2731 2763 6684 6716 2731
Size: 32 3921 32 2739
Fragments arranged by size:
3921 2739 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2114 3370 3938 1316
Size: 798 1256 568 4102
Fragments arranged by size:
4102 1256 798 568
XbaI T'CTAG_A
Cuts at: 4060 4060
Size: 6724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6724
XhoI C'TCGA_G
Cuts at: 1943 2241 1943
Size: 298 6426
XmaI C'CCGG_G
Cuts at: 1870 2280 4049 1870
Size: 410 1769 4545
Fragments arranged by size:
4545 1769 410
XmnI GAAnn'nnTTC
Cuts at: 4853 4853
Size: 6724
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4532 276
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SacII SfiI
SrfI SwaI