Restriction map for
pFN21AE5745
(Circular) (Six-base) MAPSORT of: pFN21AE5745.seq Check: 7224 from: 1 to: 6625
pFN21AE5745 6625 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2795 2795
Size: 6625
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4435 278
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3968 1937
Size: 2031 4594
Acc65I G'GTAC_C
Cuts at: 2436 3946 2436
Size: 1510 5115
AclI AA'CG_TT
Cuts at: 1563 4752 5125 1563
Size: 3189 373 3063
Fragments arranged by size:
3189 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2300 4688 6072 6340 1632
Size: 668 2388 1384 268 1917
Fragments arranged by size:
2388 1917 1384 668 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6428
AflIII A'CryG_T
Cuts at: 1203 2020 2223 3365 5524 6501 1203
Size: 817 203 1142 2159 977 1327
Fragments arranged by size:
2159 1327 1142 977 817 203
AgeI A'CCGG_T
Cuts at: 5456 5456
Size: 6625
AhdI GACnn_n'nnGTC
Cuts at: 5354 5354
Size: 6625
AleI CACnn'nnGTG
Cuts at: 1166 2333 1166
Size: 1167 5458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3779 1184
Size: 2595 4030
AlwNI CAG_nnn'CTG
Cuts at: 2452 4368 5940 2452
Size: 1916 1572 3137
Fragments arranged by size:
3137 1916 1572
ApaI G_GGCC'C
Cuts at: 2666 3127 2666
Size: 461 6164
ApaLI G'TGCA_C
Cuts at: 2628 4682 5838 2628
Size: 2054 1156 3415
Fragments arranged by size:
3415 2054 1156
ApoI r'AATT_y
Cuts at: 1493 3209 3425 3509 3934 4160 4312 6421 1493
Size: 1716 216 84 425 226 152 2109 1697
Fragments arranged by size:
2109 1716 1697 425 226 216 152 84
AseI AT'TA_AT
Cuts at: 160 1032 5179 160
Size: 872 4147 1606
Fragments arranged by size:
4147 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 2493 3248 3950 1870
Size: 73 550 755 702 4545
Fragments arranged by size:
4545 755 702 550 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2428 2461 2428
Size: 33 6592
BamHI G'GATC_C
Cuts at: 3273 3955 4322 3273
Size: 682 367 5576
Fragments arranged by size:
5576 682 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2436 3260 3946 5401 618
Size: 325 217 627 649 824 686 1455 1842
Fragments arranged by size:
1842 1455 824 686 649 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2492 2666 3036 3127 3405 3944 1391
Size: 1101 174 370 91 278 539 4072
Fragments arranged by size:
4072 1101 539 370 278 174 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2909 928
Size: 971 1010 4644
Fragments arranged by size:
4644 1010 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4816 4850 1903
Size: 34 2879 34 3678
Fragments arranged by size:
3678 2879 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4517 5727 4517
Size: 1210 5415
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6625
BfrBI ATG'CAT
Cuts at: 3820 3820
Size: 6625
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5236 136
Size: 107 122 71 631 605 3564 1525
Fragments arranged by size:
3564 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6620 6620
Size: 6625
BlpI GC'TnA_GC
Cuts at: 2816 4042 2816
Size: 1226 5399
Bme1580I G_kGCm'C
Cuts at: 1790 2632 2666 2712 3127 3263 4686 5842 1790
Size: 842 34 46 415 136 1423 1156 2573
Fragments arranged by size:
2573 1423 1156 842 415 136 46 34
BmgBI CAC'GTC
Cuts at: 1318 2342 2380 1318
Size: 1024 38 5563
Fragments arranged by size:
5563 1024 38
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4356 5314 442
Size: 416 53 83 399 24 2939 958 1753
Fragments arranged by size:
2939 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2201 1056
Size: 1145 5480
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 6625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 2558 2615 2792 2810 3050 5285 1239
Size: 1292 27 57 177 18 240 2235 2579
Fragments arranged by size:
2579 2235 1292 240 177 57 27 18
Bpu10I CC'TnA_GC
Cuts at: 3841 3841
Size: 6625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 2708 2974 4750 5615 5913 2105
Size: 603 266 1776 865 298 2817
Fragments arranged by size:
2817 1776 865 603 298 266
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3779 5288 882
Size: 617 2280 1509 2219
Fragments arranged by size:
2280 2219 1509 617
BsaAI yAC'GTr
Cuts at: 493 1204 2134 2224 2251 4416 493
Size: 711 930 90 27 2165 2702
Fragments arranged by size:
2702 2165 930 711 90 27
BsaBI GATnn'nnATC
Cuts at: 3278 6619 3278
Size: 3341 3284
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2443 2757 4432 4814
Size: 53 83 186 936 910 314 1675 382
Cuts at: 4814 5449 275
Size: 635 1451
Fragments arranged by size:
1675 1451 936 910 635 382 314 186
83 53
BsaWI w'CCGG_w
Cuts at: 5058 5456 5730 5877 6288 5058
Size: 398 274 147 411 5395
Fragments arranged by size:
5395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2622 2652 2783 2813 3752
Size: 30 165 30 1219 30 131 30 939
Cuts at: 3752 3782 1178
Size: 30 4021
Fragments arranged by size:
4021 1219 939 165 131 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 2391 2543 2567 2612 3760 1186
Size: 1009 196 152 24 45 1148 4051
Fragments arranged by size:
4051 1148 1009 196 152 45 24
BseYI C'CCAG_C
Cuts at: 5828 6324 5828
Size: 496 6129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2627 3071 3134 3212 3257 3641 1163
Size: 573 891 444 63 78 45 384 4147
Fragments arranged by size:
4147 891 573 444 384 78 63 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2146 4075 4836 5864 1540
Size: 275 186 145 1929 761 1028 2301
Fragments arranged by size:
2301 1929 1028 761 275 186 145
BsiHKAI G_wGCw'C
Cuts at: 2129 2256 2492 2632 2817 3036 4686 4771 5842
Size: 127 236 140 185 219 1650 85 1071
Cuts at: 5842 2129
Size: 2912
Fragments arranged by size:
2912 1650 1071 236 219 185 140 127
85
BsmI GAATG_Cn'
Cuts at: 4136 4229 4136
Size: 93 6532
BsmBI CGTCTCn'nnnn_
Cuts at: 2177 2332 2177
Size: 155 6470
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2256 2492 2632 2666 2712 2817
Size: 399 339 127 236 140 34 46 105
Cuts at: 2817 3036 3127 3263 3405 3944 4686 4771 5842
Size: 219 91 136 142 539 742 85 1071
Cuts at: 5842 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 539 399 339 236 219
142 140 136 127 105 91 85 46
34
BspEI T'CCGG_A
Cuts at: 6288 6288
Size: 6625
BspHI T'CATG_A
Cuts at: 4514 6244 4514
Size: 1730 4895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2795 3980 844
Size: 1951 1185 3489
Fragments arranged by size:
3489 1951 1185
BsrBI CCG'CTC
Cuts at: 1394 1823 2983 4512 1394
Size: 429 1160 1529 3507
Fragments arranged by size:
3507 1529 1160 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5120 5294 65
Size: 1183 3872 174 1396
Fragments arranged by size:
3872 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2332 2439 2740 5269 5456 1700
Size: 110 522 107 301 2529 187 2869
Fragments arranged by size:
2869 2529 522 301 187 110 107
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6625
BssHII G'CGCG_C
Cuts at: 1925 2395 1925
Size: 470 6155
BssSI C'ACGA_G
Cuts at: 4685 5697 4685
Size: 1012 5613
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2787 3235 794
Size: 1993 448 4184
Fragments arranged by size:
4184 1993 448
BstBI TT'CG_AA
Cuts at: 5429 5429
Size: 6625
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6625
BstYI r'GATC_y
Cuts at: 1972 2215 3273 3955 4322 4707 4724 6176 6262
Size: 243 1058 682 367 385 17 1452 86
Cuts at: 6262 6274 6620 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1058 682 385 367 346 243
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2403 3642 2403
Size: 1239 5386
BtgI C'CryG_G
Cuts at: 513 1065 2004 2202 2361 2745 2940 3181 3738
Size: 552 939 198 159 384 195 241 557
Cuts at: 3738 513
Size: 3400
Fragments arranged by size:
3400 939 557 552 384 241 198 195
159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5886
Fragments arranged by size:
5886 683 56
BtsI GCAGTG_nn'
Cuts at: 3442 3816 4141 4935 4955 3442
Size: 374 325 794 20 5112
Fragments arranged by size:
5112 794 374 325 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6590
DraI TTT'AAA
Cuts at: 3929 3929
Size: 6625
DraIII CAC_nnn'GTG
Cuts at: 3157 3235 3157
Size: 78 6547
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2356 5632 809
Size: 507 1040 3276 1802
Fragments arranged by size:
3276 1802 1040 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2457 2604
Size: 54 1425 50 275 18 313 314 147
Cuts at: 2604 2742 2937 3504 3682 4072 4961 6590 8
Size: 138 195 567 178 390 889 1629 43
Fragments arranged by size:
1629 1425 889 567 390 314 313 275
195 178 147 138 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2143 4072 1537
Size: 275 331 1929 4090
Fragments arranged by size:
4090 1929 331 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2480 3101 4555 1353
Size: 1127 621 1454 3423
Fragments arranged by size:
3423 1454 1127 621
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2362 5212 5586 5732 387
Size: 1064 138 773 2850 374 146 1280
Fragments arranged by size:
2850 1280 1064 773 374 146 138
EcoICRI GAG'CTC
Cuts at: 2490 3034 2490
Size: 544 6081
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2531 2558 2615 2792 2810 3050
Size: 393 668 231 27 57 177 18 240
Cuts at: 3050 4688 5285 6072 6340 1239
Size: 1638 597 787 268 1524
Fragments arranged by size:
1638 1524 787 668 597 393 268 240
231 177 57 27 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2240 2240
Size: 6625
EcoO109I rG'GnC_Cy
Cuts at: 2275 2533 3009 3124 3412 3526 2275
Size: 258 476 115 288 114 5374
Fragments arranged by size:
5374 476 288 258 115 114
EcoRI G'AATT_C
Cuts at: 3425 3934 4312 3425
Size: 509 378 5738
Fragments arranged by size:
5738 509 378
FspI TGC'GCA
Cuts at: 2771 5131 2771
Size: 2360 4265
HaeII r_GCGC'y
Cuts at: 1678 2452 5772 1678
Size: 774 3320 2531
Fragments arranged by size:
3320 2531 774
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3752 3784 5266 5298 5340
Size: 32 135 32 2180 32 1482 32 42
Cuts at: 5340 5372 1373
Size: 32 2626
Fragments arranged by size:
2626 2180 1482 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3969 4215 669
Size: 988 281 2031 246 3079
Fragments arranged by size:
3079 2031 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6625
HpaI GTT'AAC
Cuts at: 4215 4215
Size: 6625
KpnI G_GTAC'C
Cuts at: 2440 3950 2440
Size: 1510 5115
MfeI C'AATT_G
Cuts at: 4224 4224
Size: 6625
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3002 5739 5923 1305
Size: 1697 2737 184 2007
Fragments arranged by size:
2737 2007 1697 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2459 2606 3506 10
Size: 54 1425 343 627 147 900 3129
Fragments arranged by size:
3129 1425 900 627 343 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 3497 4583 4942 5101 518
Size: 648 1167 1164 1086 359 159 2042
Fragments arranged by size:
2042 1167 1164 1086 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2518 3183 3349 4041 4718 5866 6111 1606
Size: 912 665 166 692 677 1148 245 2120
Fragments arranged by size:
2120 1148 912 692 677 665 245 166
NaeI GCC'GGC
Cuts at: 2742 2742
Size: 6625
NcoI C'CATG_G
Cuts at: 513 1065 2004 2202 2361 2745 3738 513
Size: 552 939 198 159 384 993 3400
Fragments arranged by size:
3400 993 939 552 384 198 159
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6625
NgoMIV G'CCGG_C
Cuts at: 2740 2740
Size: 6625
NheI G'CTAG_C
Cuts at: 1052 2197 1052
Size: 1145 5480
NotI GC'GGCC_GC
Cuts at: 2143 4072 2143
Size: 1929 4696
NsiI A_TGCA'T
Cuts at: 3822 3822
Size: 6625
NspI r_CATG'y
Cuts at: 1132 1229 2024 2979 3369 3766 3983 5528 1132
Size: 97 795 955 390 397 217 1545 2229
Fragments arranged by size:
2229 1545 955 795 397 390 217 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6625
PciI A'CATG_T
Cuts at: 2020 3365 5524 2020
Size: 1345 2159 3121
Fragments arranged by size:
3121 2159 1345
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6625
PfoI T'CCnGG_A
Cuts at: 2211 3113 2211
Size: 902 5723
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PmlI CAC'GTG
Cuts at: 2134 2134
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 6207 6239 4694
Size: 32 1481 32 5080
Fragments arranged by size:
5080 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 3412 2275
Size: 1137 5488
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6625
PsiI TTA'TAA
Cuts at: 4195 4195
Size: 6625
PspOMI G'GGCC_C
Cuts at: 2662 3123 2662
Size: 461 6164
PspXI vC'TCGA_Gb
Cuts at: 3248 3248
Size: 6625
PstI C_TGCA'G
Cuts at: 830 3758 3977 830
Size: 2928 219 3478
Fragments arranged by size:
3478 2928 219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
PvuII CAG'CTG
Cuts at: 3349 3349
Size: 6625
RsrII CG'GwC_CG
Cuts at: 2308 2308
Size: 6625
SacI G_AGCT'C
Cuts at: 2492 3036 2492
Size: 544 6081
SacII CC_GC'GG
Cuts at: 3184 3184
Size: 6625
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SapI GCTCTTCn'nnn_
Cuts at: 2480 2480
Size: 6625
SbfI CC_TGCA'GG
Cuts at: 3977 3977
Size: 6625
ScaI AGT'ACT
Cuts at: 4873 6585 4873
Size: 1712 4913
SexAI A'CCwGG_T
Cuts at: 3726 3726
Size: 6625
SfcI C'TryA_G
Cuts at: 826 1045 3631 3754 3973 5108 5789 5980 826
Size: 219 2586 123 219 1135 681 191 1471
Fragments arranged by size:
2586 1471 1135 681 219 219 191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 2332 1700
Size: 632 5993
SmaI CCC'GGG
Cuts at: 1872 2495 3952 1872
Size: 623 1457 4545
Fragments arranged by size:
4545 1457 623
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 2723 2953 3248 4729 5630
Size: 197 926 177 603 230 295 1481 901
Cuts at: 5630 5892 820
Size: 262 1553
Fragments arranged by size:
1553 1481 926 901 603 295 262 230
197 177
SnaBI TAC'GTA
Cuts at: 493 4416 493
Size: 3923 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6625
SphI G_CATG'C
Cuts at: 1132 2979 3983 1132
Size: 1847 1004 3774
Fragments arranged by size:
3774 1847 1004
SspI AAT'ATT
Cuts at: 5 52 4549 5
Size: 47 4497 2081
Fragments arranged by size:
4497 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6625
StyI C'CwwG_G
Cuts at: 513 1065 2004 2202 2361 2745 3738 4064 513
Size: 552 939 198 159 384 993 326 3074
Fragments arranged by size:
3074 993 939 552 384 326 198 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4652 4669 4822 5007 5452 325
Size: 1255 3072 17 153 185 445 1498
Fragments arranged by size:
3072 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4871 6583 96
Size: 274 80 33 51 1443 2894 1712 138
Fragments arranged by size:
2894 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2622 2654 3258 3290 6585 6617 2622
Size: 32 604 32 3295 32 2630
Fragments arranged by size:
3295 2630 604 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2381 1316
Size: 1065 5560
XbaI T'CTAG_A
Cuts at: 3961 3961
Size: 6625
XhoI C'TCGA_G
Cuts at: 1943 3248 1943
Size: 1305 5320
XmaI C'CCGG_G
Cuts at: 1870 2493 3950 1870
Size: 623 1457 4545
Fragments arranged by size:
4545 1457 623
XmnI GAAnn'nnTTC
Cuts at: 4754 4754
Size: 6625
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4433 276
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BsiWI BstXI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
PacI PsrI SanDI SfiI SfoI SrfI SwaI XcmI