Restriction map for
pFN21AE5742
(Circular) (Six-base) MAPSORT of: pFN21AE5742.seq Check: 6825 from: 1 to: 6739
pFN21AE5742 6739 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2776 2776
Size: 6739
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4549 278
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2427 3438 4082 1937
Size: 490 1011 644 4594
Fragments arranged by size:
4594 1011 644 490
Acc65I G'GTAC_C
Cuts at: 4060 4060
Size: 6739
AclI AA'CG_TT
Cuts at: 1563 4866 5239 1563
Size: 3303 373 3063
Fragments arranged by size:
3303 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2723 2810 3477 3494 4802 6186 6454 1632
Size: 1091 87 667 17 1308 1384 268 1917
Fragments arranged by size:
1917 1384 1308 1091 667 268 87 17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6542
AflIII A'CryG_T
Cuts at: 1203 2583 3441 5638 6615 1203
Size: 1380 858 2197 977 1327
Fragments arranged by size:
2197 1380 1327 977 858
AgeI A'CCGG_T
Cuts at: 5570 5570
Size: 6739
AhdI GACnn_n'nnGTC
Cuts at: 5468 5468
Size: 6739
AleI CACnn'nnGTG
Cuts at: 1166 3320 1166
Size: 2154 4585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2586 3725 3935 4023 1184
Size: 1402 1139 210 88 3900
Fragments arranged by size:
3900 1402 1139 210 88
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2448 2480 2448
Size: 32 6707
AlwNI CAG_nnn'CTG
Cuts at: 2494 2519 2965 3181 3211 3527 3846 4482 6054
Size: 25 446 216 30 316 319 636 1572
Cuts at: 6054 2494
Size: 3179
Fragments arranged by size:
3179 1572 636 446 319 316 216 30
25
ApaI G_GGCC'C
Cuts at: 3205 3205
Size: 6739
ApaLI G'TGCA_C
Cuts at: 2174 4796 5952 2174
Size: 2622 1156 2961
Fragments arranged by size:
2961 2622 1156
ApoI r'AATT_y
Cuts at: 1493 2759 4048 4274 4426 6535 1493
Size: 1266 1289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1289 1266 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5293 160
Size: 872 4261 1606
Fragments arranged by size:
4261 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2345 3108 3420 4064 1870
Size: 73 154 248 763 312 644 4545
Fragments arranged by size:
4545 763 644 312 248 154 73
BamHI G'GATC_C
Cuts at: 2683 3781 3942 4069 4436 2683
Size: 1098 161 127 367 4986
Fragments arranged by size:
4986 1098 367 161 127
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2124 2289 3725 4060
Size: 325 217 627 297 40 165 1436 335
Cuts at: 4060 5515 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1436 627 335 325 297 217
165 40
BanII G_rGCy'C
Cuts at: 1391 2370 3205 3378 4058 1391
Size: 979 835 173 680 4072
Fragments arranged by size:
4072 979 835 680 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2099 2368 3796 928
Size: 971 200 269 1428 3871
Fragments arranged by size:
3871 1428 971 269 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4930 4964 1903
Size: 34 2993 34 3678
Fragments arranged by size:
3678 2993 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4631 5841 4631
Size: 1210 5529
BclI T'GATC_A
Cuts at: 1548 2034 3486 1548
Size: 486 1452 4801
Fragments arranged by size:
4801 1452 486
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3026 5350 136
Size: 107 122 71 631 605 1354 2324 1525
Fragments arranged by size:
2324 1525 1354 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6734 6734
Size: 6739
BlpI GC'TnA_GC
Cuts at: 4156 4156
Size: 6739
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2476 3205 3421 3728 3972 4800 5956
Size: 388 298 729 216 307 244 828 1156
Cuts at: 5956 1790
Size: 2573
Fragments arranged by size:
2573 1156 828 729 388 307 298 244
216
BmgBI CAC'GTC
Cuts at: 1318 2557 2947 1318
Size: 1239 390 5110
Fragments arranged by size:
5110 1239 390
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3641 4470 5428
Size: 416 53 83 399 24 2224 829 958
Cuts at: 5428 442
Size: 1753
Fragments arranged by size:
2224 1753 958 829 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2450 2756 3077 3149 3521 3760 3944
Size: 830 381 306 321 72 372 239 184
Cuts at: 3944 5399 1239
Size: 1455 2579
Fragments arranged by size:
2579 1455 830 381 372 321 306 239
184 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4864 5729 6027 4864
Size: 865 298 5576
Fragments arranged by size:
5576 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2504 3920 5402 882
Size: 617 1005 1416 1482 2219
Fragments arranged by size:
2219 1482 1416 1005 617
BsaAI yAC'GTr
Cuts at: 493 1204 2264 2362 2586 3658 4530 493
Size: 711 1060 98 224 1072 872 2702
Fragments arranged by size:
2702 1072 1060 872 711 224 98
BsaBI GATnn'nnATC
Cuts at: 2931 6733 2931
Size: 3802 2937
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4546 4928 5563 275
Size: 53 83 186 936 3013 382 635 1451
Fragments arranged by size:
3013 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5172 5570 5844 5991 6402 5172
Size: 398 274 147 411 5509
Fragments arranged by size:
5509 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2136 2166 2448
Size: 30 165 30 597 30 106 30 282
Cuts at: 2448 2478 3135 3165 1178
Size: 30 657 30 4752
Fragments arranged by size:
4752 657 597 282 165 106 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 2366 2369 3344 1186
Size: 1177 3 3 975 4581
Fragments arranged by size:
4581 1177 975 3 3
BseYI C'CCAG_C
Cuts at: 2187 2494 2619 2819 3204 3221 3618 5942 6438
Size: 307 125 200 385 17 397 2324 496
Cuts at: 6438 2187
Size: 2488
Fragments arranged by size:
2488 2324 496 397 385 307 200 125
17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2293 3554 3680 1163
Size: 573 557 1261 126 4222
Fragments arranged by size:
4222 1261 573 557 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4189 4950 5978 1540
Size: 275 186 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2178 2361 2370 2634 2946 3242 3657 3903 4800
Size: 183 9 264 312 296 415 246 897
Cuts at: 4800 4885 5956 2178
Size: 85 1071 2961
Fragments arranged by size:
2961 1071 897 415 312 296 264 246
183 85 9
BsmI GAATG_Cn'
Cuts at: 4250 4343 4250
Size: 93 6646
BsmBI CGTCTCn'nnnn_
Cuts at: 3497 3497
Size: 6739
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2361 2370 2476 2634 2946 3205
Size: 399 388 183 9 106 158 312 259
Cuts at: 3205 3242 3378 3421 3657 3728 3903 3972 4058
Size: 37 136 43 236 71 175 69 86
Cuts at: 4058 4800 4885 5956 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 388 312 259 236
183 175 158 136 106 86 85 71
69 43 37 9
BspEI T'CCGG_A
Cuts at: 6402 6402
Size: 6739
BspHI T'CATG_A
Cuts at: 2439 4628 6358 2439
Size: 2189 1730 2820
Fragments arranged by size:
2820 2189 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2307 2776 3717 4094 844
Size: 1463 469 941 377 3489
Fragments arranged by size:
3489 1463 941 469 377
BsrBI CCG'CTC
Cuts at: 1394 1823 4626 1394
Size: 429 2803 3507
Fragments arranged by size:
3507 2803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3349 3361 5234 5408 65
Size: 1183 2101 12 1873 174 1396
Fragments arranged by size:
2101 1873 1396 1183 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2979 5383 5570 1700
Size: 110 1169 2404 187 2869
Fragments arranged by size:
2869 2404 1169 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6739
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6739
BssSI C'ACGA_G
Cuts at: 3316 4799 5811 3316
Size: 1483 1012 4244
Fragments arranged by size:
4244 1483 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3181 3527 3678 794
Size: 2387 346 151 3855
Fragments arranged by size:
3855 2387 346 151
BstBI TT'CG_AA
Cuts at: 5543 5543
Size: 6739
BstEII G'GTnAC_C
Cuts at: 1184 2182 1184
Size: 998 5741
BstXI CCAn_nnnn'nTGG
Cuts at: 3322 3322
Size: 6739
BstYI r'GATC_y
Cuts at: 1972 2683 2961 3072 3567 3574 3781 3942 4069
Size: 711 278 111 495 7 207 161 127
Cuts at: 4069 4436 4821 4838 6290 6376 6388 6734 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 711 495 385 367 346 278
207 161 127 111 86 17 12 7
BstZ17I GTA'TAC
Cuts at: 3439 3439
Size: 6739
Bsu36I CC'TnA_GG
Cuts at: 2855 3933 2855
Size: 1078 5661
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 2901 3396 3697 513
Size: 552 939 75 822 495 301 3555
Fragments arranged by size:
3555 939 822 552 495 301 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6000
Fragments arranged by size:
6000 683 56
BtsI GCAGTG_nn'
Cuts at: 3528 4255 5049 5069 3528
Size: 727 794 20 5198
Fragments arranged by size:
5198 794 727 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6739
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6704
DraI TTT'AAA
Cuts at: 4043 4043
Size: 6739
DraIII CAC_nnn'GTG
Cuts at: 2182 2589 2182
Size: 407 6332
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5746 809
Size: 507 4430 1802
Fragments arranged by size:
4430 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2275 3312
Size: 54 1425 50 275 18 285 160 1037
Cuts at: 3312 4186 5075 6704 8
Size: 874 889 1629 43
Fragments arranged by size:
1629 1425 1037 889 874 285 275 160
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4186 1537
Size: 275 2374 4090
Fragments arranged by size:
4090 2374 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2193 4669 1353
Size: 840 2476 3423
Fragments arranged by size:
3423 2476 840
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3341 3955 5326 5700 5846 387
Size: 1064 138 1752 614 1371 374 146 1280
Fragments arranged by size:
1752 1371 1280 1064 614 374 146 138
EcoICRI GAG'CTC
Cuts at: 2368 2368
Size: 6739
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2450 2723 2756 2810 3077 3149
Size: 393 437 381 273 33 54 267 72
Cuts at: 3149 3477 3494 3521 3760 3944 4802 5399 6186
Size: 328 17 27 239 184 858 597 787
Cuts at: 6186 6454 1239
Size: 268 1524
Fragments arranged by size:
1524 858 787 597 437 393 381 328
273 268 267 239 184 72 54 33
27 17
EcoNI CCTnn'n_nnAGG
Cuts at: 3713 3713
Size: 6739
EcoO109I rG'GnC_Cy
Cuts at: 2526 3178 2526
Size: 652 6087
EcoRI G'AATT_C
Cuts at: 4048 4426 4048
Size: 378 6361
FspI TGC'GCA
Cuts at: 5245 5245
Size: 6739
HaeII r_GCGC'y
Cuts at: 1678 2863 2980 3500 5886 1678
Size: 1185 117 520 2386 2531
Fragments arranged by size:
2531 2386 1185 520 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 3559 3591 4004
Size: 32 135 32 428 32 1527 32 413
Cuts at: 4004 4036 5380 5412 5454 5486 1373
Size: 32 1344 32 42 32 2626
Fragments arranged by size:
2626 1527 1344 428 413 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2167 4083 4329 669
Size: 988 281 229 1916 246 3079
Fragments arranged by size:
3079 1916 988 281 246 229
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6739
HpaI GTT'AAC
Cuts at: 4329 4329
Size: 6739
KpnI G_GTAC'C
Cuts at: 4064 4064
Size: 6739
MfeI C'AATT_G
Cuts at: 4338 4338
Size: 6739
MluI A'CGCG_T
Cuts at: 3441 6615 3441
Size: 3174 3565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4002 5853 6037 1305
Size: 2697 1851 184 2007
Fragments arranged by size:
2697 2007 1851 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 2277 3314 10
Size: 54 1425 343 285 160 1037 3435
Fragments arranged by size:
3435 1425 1037 343 285 160 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3245 3320 3354 3738 4697 5056 5215
Size: 648 2079 75 34 384 959 359 159
Cuts at: 5215 518
Size: 2042
Fragments arranged by size:
2079 2042 959 648 384 359 159 75
34
MspA1I CmG'CkG
Cuts at: 1606 2623 3622 3634 3837 4155 4832 5980 6225
Size: 1017 999 12 203 318 677 1148 245
Cuts at: 6225 1606
Size: 2120
Fragments arranged by size:
2120 1148 1017 999 677 318 245 203
12
NaeI GCC'GGC
Cuts at: 2981 2981
Size: 6739
NcoI C'CATG_G
Cuts at: 513 1065 2004 3396 3697 513
Size: 552 939 1392 301 3555
Fragments arranged by size:
3555 1392 939 552 301
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6739
NgoMIV G'CCGG_C
Cuts at: 2979 2979
Size: 6739
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6739
NotI GC'GGCC_GC
Cuts at: 4186 4186
Size: 6739
NspI r_CATG'y
Cuts at: 1132 1229 2215 3919 4097 5642 1132
Size: 97 986 1704 178 1545 2229
Fragments arranged by size:
2229 1704 1545 986 178 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6739
PciI A'CATG_T
Cuts at: 5638 5638
Size: 6739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2267 2494 2519 3957 1108
Size: 1159 227 25 1438 3890
Fragments arranged by size:
3890 1438 1159 227 25
PfoI T'CCnGG_A
Cuts at: 3570 3570
Size: 6739
PmeI GTTT'AAAC
Cuts at: 4043 4043
Size: 6739
PmlI CAC'GTG
Cuts at: 2264 2362 2586 3658 2264
Size: 98 224 1072 5345
Fragments arranged by size:
5345 1072 224 98
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2448 2480 4808 4840 6321 6353 2448
Size: 32 2328 32 1481 32 2834
Fragments arranged by size:
2834 2328 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6739
PsiI TTA'TAA
Cuts at: 4309 4309
Size: 6739
PspOMI G'GGCC_C
Cuts at: 3201 3201
Size: 6739
PstI C_TGCA'G
Cuts at: 830 2328 2565 2628 2925 3186 3582 3842 3870
Size: 1498 237 63 297 261 396 260 28
Cuts at: 3870 4002 4091 830
Size: 132 89 3478
Fragments arranged by size:
3478 1498 396 297 261 260 237 132
89 63 28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6739
PvuII CAG'CTG
Cuts at: 2623 3837 2623
Size: 1214 5525
SacI G_AGCT'C
Cuts at: 2370 2370
Size: 6739
SalI G'TCGA_C
Cuts at: 1936 4081 1936
Size: 2145 4594
SbfI CC_TGCA'GG
Cuts at: 4091 4091
Size: 6739
ScaI AGT'ACT
Cuts at: 4987 6699 4987
Size: 1712 5027
SexAI A'CCwGG_T
Cuts at: 2178 2178
Size: 6739
SfcI C'TryA_G
Cuts at: 826 1045 2324 2561 2624 2921 3182 3578 3838
Size: 219 1279 237 63 297 261 396 260
Cuts at: 3838 3866 3998 4087 5222 5903 6094 826
Size: 28 132 89 1135 681 191 1471
Fragments arranged by size:
1471 1279 1135 681 396 297 261 260
237 219 191 132 89 63 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6739
SmaI CCC'GGG
Cuts at: 1872 2099 3422 4066 1872
Size: 227 1323 644 4545
Fragments arranged by size:
4545 1323 644 227
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4843 5744 6006 820
Size: 197 926 2900 901 262 1553
Fragments arranged by size:
2900 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4530 493
Size: 4037 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6739
SphI G_CATG'C
Cuts at: 1132 2215 3919 4097 1132
Size: 1083 1704 178 3774
Fragments arranged by size:
3774 1704 1083 178
SrfI GCCC'GGGC
Cuts at: 3422 3422
Size: 6739
SspI AAT'ATT
Cuts at: 5 52 4663 5
Size: 47 4611 2081
Fragments arranged by size:
4611 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6739
StyI C'CwwG_G
Cuts at: 513 1065 2004 2148 2910 3396 3697 4178 513
Size: 552 939 144 762 486 301 481 3074
Fragments arranged by size:
3074 939 762 552 486 481 301 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2004 3257 3743 4766 4783 4936 5121
Size: 1255 424 1253 486 1023 17 153 185
Cuts at: 5121 5566 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1253 1023 486 445 424 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 4985 6697
Size: 274 80 33 51 1443 678 2330 1712
Cuts at: 6697 96
Size: 138
Fragments arranged by size:
2330 1712 1443 678 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2136 2168 2573 2605 3135 3167 6699 6731 2136
Size: 32 405 32 530 32 3532 32 2144
Fragments arranged by size:
3532 2144 530 405 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2423 2891 1316
Size: 1107 468 5164
Fragments arranged by size:
5164 1107 468
XbaI T'CTAG_A
Cuts at: 4075 4075
Size: 6739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2687 3212 3784 2687
Size: 525 572 5642
Fragments arranged by size:
5642 572 525
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6739
XmaI C'CCGG_G
Cuts at: 1870 2097 3420 4064 1870
Size: 227 1323 644 4545
Fragments arranged by size:
4545 1323 644 227
XmnI GAAnn'nnTTC
Cuts at: 4868 4868
Size: 6739
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4547 276
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SfiI SfoI SwaI