Restriction map for
pFN21AE5740
(Circular) (Six-base) MAPSORT of: pFN21AE5740.seq Check: 9277 from: 1 to: 6811
pFN21AE5740 6811 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3765 4621 278
Size: 53 83 186 936 2229 856 2468
Fragments arranged by size:
2468 2229 936 856 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2510 4154 1937
Size: 573 1644 4594
Fragments arranged by size:
4594 1644 573
Acc65I G'GTAC_C
Cuts at: 4132 4132
Size: 6811
AclI AA'CG_TT
Cuts at: 1563 4938 5311 1563
Size: 3375 373 3063
Fragments arranged by size:
3375 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2813 4874 6258 6526 1632
Size: 1181 2061 1384 268 1917
Fragments arranged by size:
2061 1917 1384 1181 268
AfeI AGC'GCT
Cuts at: 3190 3190
Size: 6811
AflII C'TTAA_G
Cuts at: 820 1017 3953 820
Size: 197 2936 3678
Fragments arranged by size:
3678 2936 197
AflIII A'CryG_T
Cuts at: 1203 5710 6687 1203
Size: 4507 977 1327
Fragments arranged by size:
4507 1327 977
AgeI A'CCGG_T
Cuts at: 2347 5642 2347
Size: 3295 3516
AhdI GACnn_n'nnGTC
Cuts at: 5540 5540
Size: 6811
AleI CACnn'nnGTG
Cuts at: 1166 3881 1166
Size: 2715 4096
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3068 1184
Size: 1884 4927
AlwNI CAG_nnn'CTG
Cuts at: 4554 6126 4554
Size: 1572 5239
ApaI G_GGCC'C
Cuts at: 2366 3786 2366
Size: 1420 5391
ApaLI G'TGCA_C
Cuts at: 4868 6024 4868
Size: 1156 5655
ApoI r'AATT_y
Cuts at: 1493 2593 2644 3026 3440 3555 4120 4346 4498
Size: 1100 51 382 414 115 565 226 152
Cuts at: 4498 6607 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1100 565 414 382 226 152
115 51
AseI AT'TA_AT
Cuts at: 160 1032 5365 160
Size: 872 4333 1606
Fragments arranged by size:
4333 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2114 2288 2317 4136 1870
Size: 73 71 100 174 29 1819 4545
Fragments arranged by size:
4545 1819 174 100 73 71 29
BamHI G'GATC_C
Cuts at: 2921 4141 4508 2921
Size: 1220 367 5224
Fragments arranged by size:
5224 1220 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2142 4132 5587 618
Size: 325 217 627 355 1990 1455 1842
Fragments arranged by size:
1990 1842 1455 627 355 325 217
BanII G_rGCy'C
Cuts at: 1391 2203 2366 3252 3786 4130 1391
Size: 812 163 886 534 344 4072
Fragments arranged by size:
4072 886 812 534 344 163
BbeI G_GCGC'C
Cuts at: 2146 2146
Size: 6811
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2975 4090 928
Size: 971 1076 1115 3649
Fragments arranged by size:
3649 1115 1076 971
BbvCI CC'TCA_GC
Cuts at: 2090 2090
Size: 6811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5002 5036 1903
Size: 34 3065 34 3678
Fragments arranged by size:
3678 3065 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2540 3764 4703 5913 2540
Size: 1224 939 1210 3438
Fragments arranged by size:
3438 1224 1210 939
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6811
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 2442 5422
Size: 107 122 71 631 605 502 268 2980
Cuts at: 5422 136
Size: 1525
Fragments arranged by size:
2980 1525 631 605 502 268 122 107
71
BglII A'GATC_T
Cuts at: 3565 6806 3565
Size: 3241 3570
BlpI GC'TnA_GC
Cuts at: 4228 4228
Size: 6811
Bme1580I G_kGCm'C
Cuts at: 1790 2366 3786 3888 4872 6028 1790
Size: 576 1420 102 984 1156 2573
Fragments arranged by size:
2573 1420 1156 984 576 102
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6811
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4542 5500 442
Size: 416 53 83 399 24 3125 958 1753
Fragments arranged by size:
3125 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6811
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2772 2772
Size: 6811
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2209 4041 5471 1239
Size: 970 1832 1430 2579
Fragments arranged by size:
2579 1832 1430 970
Bpu10I CC'TnA_GC
Cuts at: 2090 3587 3899 2090
Size: 1497 312 5002
Fragments arranged by size:
5002 1497 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2545 2806 3598 4936 5801 6099 2545
Size: 261 792 1338 865 298 3257
Fragments arranged by size:
3257 1338 865 792 298 261
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2227 5474 882
Size: 617 728 3247 2219
Fragments arranged by size:
3247 2219 728 617
BsaAI yAC'GTr
Cuts at: 493 1204 3295 4602 493
Size: 711 2091 1307 2702
Fragments arranged by size:
2702 2091 1307 711
BsaBI GATnn'nnATC
Cuts at: 6805 6805
Size: 6811
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2143 3762 4618 5000
Size: 53 83 186 936 610 1619 856 382
Cuts at: 5000 5635 275
Size: 635 1451
Fragments arranged by size:
1619 1451 936 856 635 610 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2347 3675 5244 5642 5916 6063 6474 2347
Size: 1328 1569 398 274 147 411 2684
Fragments arranged by size:
2684 1569 1328 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6586
Fragments arranged by size:
6586 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2327 2643 4070 1186
Size: 1141 316 1427 3927
Fragments arranged by size:
3927 1427 1141 316
BseYI C'CCAG_C
Cuts at: 2079 2252 3287 3732 6014 6510 2079
Size: 173 1035 445 2282 496 2380
Fragments arranged by size:
2380 2282 1035 496 445 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2471 1163
Size: 573 735 5503
Fragments arranged by size:
5503 735 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2137 2178 2371 2394 4261 5022
Size: 275 186 136 41 193 23 1867 761
Cuts at: 5022 6050 1540
Size: 1028 2301
Fragments arranged by size:
2301 1867 1028 761 275 193 186 136
41 23
BsiHKAI G_wGCw'C
Cuts at: 4872 4957 6028 4872
Size: 85 1071 5655
Fragments arranged by size:
5655 1071 85
BsmI GAATG_Cn'
Cuts at: 3515 4322 4415 3515
Size: 807 93 5911
Fragments arranged by size:
5911 807 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2203 2366 3252 3786 3888 4130 4872
Size: 399 413 163 886 534 102 242 742
Cuts at: 4872 4957 6028 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 886 742 534 413 399 242
163 102 85
BspEI T'CCGG_A
Cuts at: 3675 6474 3675
Size: 2799 4012
BspHI T'CATG_A
Cuts at: 4700 6430 4700
Size: 1730 5081
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2443 4166 844
Size: 1599 1723 3489
Fragments arranged by size:
3489 1723 1599
BsrBI CCG'CTC
Cuts at: 1394 1823 3309 4698 1394
Size: 429 1486 1389 3507
Fragments arranged by size:
3507 1486 1389 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5306 5480 65
Size: 1183 4058 174 1396
Fragments arranged by size:
4058 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2097 2109 2177 2328 2347 2389 5455
Size: 110 287 12 68 151 19 42 3066
Cuts at: 5455 5642 1700
Size: 187 2869
Fragments arranged by size:
3066 2869 287 187 151 110 68 42
19 12
BsrGI T'GTAC_A
Cuts at: 96 3372 96
Size: 3276 3535
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6811
BssSI C'ACGA_G
Cuts at: 4871 5883 4871
Size: 1012 5799
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3425 794
Size: 2631 4180
BstBI TT'CG_AA
Cuts at: 5615 5615
Size: 6811
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6811
BstXI CCAn_nnnn'nTGG
Cuts at: 2933 3295 2933
Size: 362 6449
BstYI r'GATC_y
Cuts at: 1972 2418 2921 3565 3750 4141 4508 4893 4910
Size: 446 503 644 185 391 367 385 17
Cuts at: 4910 6362 6448 6460 6806 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 644 503 446 391 385 367
346 185 86 17 12
BstZ17I GTA'TAC
Cuts at: 2511 2511
Size: 6811
Bsu36I CC'TnA_GG
Cuts at: 3098 4053 3098
Size: 955 5856
BtgI C'CryG_G
Cuts at: 513 1065 2004 2358 513
Size: 552 939 354 4966
Fragments arranged by size:
4966 939 552 354
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6072
Fragments arranged by size:
6072 683 56
BtsI GCAGTG_nn'
Cuts at: 4327 5121 5141 4327
Size: 794 20 5997
Fragments arranged by size:
5997 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6811
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6776
DraI TTT'AAA
Cuts at: 4012 4115 4012
Size: 103 6708
DraIII CAC_nnn'GTG
Cuts at: 3947 3947
Size: 6811
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5818 809
Size: 507 4502 1802
Fragments arranged by size:
4502 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2368 2443
Size: 54 1425 50 275 18 345 193 75
Cuts at: 2443 4258 5147 6776 8
Size: 1815 889 1629 43
Fragments arranged by size:
1815 1629 1425 889 345 275 193 75
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2175 2368 4258 1537
Size: 275 363 193 1890 4090
Fragments arranged by size:
4090 1890 363 275 193
EarI CTCTTCn'nnn_
Cuts at: 1353 2800 2856 3888 4741 1353
Size: 1447 56 1032 853 3423
Fragments arranged by size:
3423 1447 1032 853 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2302 5398 5772 5918 387
Size: 1064 138 435 278 3096 374 146 1280
Fragments arranged by size:
3096 1280 1064 435 374 278 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2209 2813 4041 4874 5471 6258 6526
Size: 393 577 604 1228 833 597 787 268
Cuts at: 6526 1239
Size: 1524
Fragments arranged by size:
1524 1228 833 787 604 597 577 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2363 3652 2363
Size: 1289 5522
EcoRI G'AATT_C
Cuts at: 4120 4498 4120
Size: 378 6433
FspI TGC'GCA
Cuts at: 5317 5317
Size: 6811
HaeII r_GCGC'y
Cuts at: 1678 2146 3192 5958 1678
Size: 468 1046 2766 2531
Fragments arranged by size:
2766 2531 1046 468
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2486 2518 3385 3417 3631
Size: 32 135 32 914 32 867 32 214
Cuts at: 3631 3663 5452 5484 5526 5558 1373
Size: 32 1789 32 42 32 2626
Fragments arranged by size:
2626 1789 914 867 214 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2835 2943 2995 3220 4155 4401
Size: 988 281 897 108 52 225 935 246
Cuts at: 4401 669
Size: 3079
Fragments arranged by size:
3079 988 935 897 281 246 225 108
52
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6811
HpaI GTT'AAC
Cuts at: 2835 4401 2835
Size: 1566 5245
KasI G'GCGC_C
Cuts at: 2142 2142
Size: 6811
KpnI G_GTAC'C
Cuts at: 4136 4136
Size: 6811
MfeI C'AATT_G
Cuts at: 4410 4410
Size: 6811
MluI A'CGCG_T
Cuts at: 6687 6687
Size: 6811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2777 2914 3904 5925 6109 1305
Size: 1472 137 990 2021 184 2007
Fragments arranged by size:
2021 2007 1472 990 184 137
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2445 10
Size: 54 1425 343 613 4376
Fragments arranged by size:
4376 1425 613 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 3690 3848 3881 4769 5128 5287
Size: 648 1572 952 158 33 888 359 159
Cuts at: 5287 518
Size: 2042
Fragments arranged by size:
2042 1572 952 888 648 359 159 158
33
MspA1I CmG'CkG
Cuts at: 1606 3595 3732 4227 4904 6052 6297 1606
Size: 1989 137 495 677 1148 245 2120
Fragments arranged by size:
2120 1989 1148 677 495 245 137
NaeI GCC'GGC
Cuts at: 2099 2111 2179 2099
Size: 12 68 6731
Fragments arranged by size:
6731 68 12
NarI GG'CG_CC
Cuts at: 2143 2143
Size: 6811
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5320
Fragments arranged by size:
5320 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6811
NgoMIV G'CCGG_C
Cuts at: 2097 2109 2177 2097
Size: 12 68 6731
Fragments arranged by size:
6731 68 12
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6811
NotI GC'GGCC_GC
Cuts at: 4258 4258
Size: 6811
NspI r_CATG'y
Cuts at: 1132 1229 4169 5714 1132
Size: 97 2940 1545 2229
Fragments arranged by size:
2940 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6811
PciI A'CATG_T
Cuts at: 5710 5710
Size: 6811
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6811
PfoI T'CCnGG_A
Cuts at: 3753 4018 3753
Size: 265 6546
PmeI GTTT'AAAC
Cuts at: 4115 4115
Size: 6811
PmlI CAC'GTG
Cuts at: 3295 3295
Size: 6811
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2289 2321 4880 4912 6393 6425 2289
Size: 32 2559 32 1481 32 2675
Fragments arranged by size:
2675 2559 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3652 3652
Size: 6811
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6811
PsiI TTA'TAA
Cuts at: 4381 4381
Size: 6811
PspOMI G'GGCC_C
Cuts at: 2362 3782 2362
Size: 1420 5391
PspXI vC'TCGA_Gb
Cuts at: 2317 2317
Size: 6811
PstI C_TGCA'G
Cuts at: 830 3600 4163 830
Size: 2770 563 3478
Fragments arranged by size:
3478 2770 563
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6811
PvuII CAG'CTG
Cuts at: 3595 3732 3595
Size: 137 6674
SalI G'TCGA_C
Cuts at: 1936 4153 1936
Size: 2217 4594
SapI GCTCTTCn'nnn_
Cuts at: 3888 3888
Size: 6811
SbfI CC_TGCA'GG
Cuts at: 4163 4163
Size: 6811
ScaI AGT'ACT
Cuts at: 5059 6771 5059
Size: 1712 5099
SfcI C'TryA_G
Cuts at: 826 1045 3596 3835 4159 5294 5975 6166 826
Size: 219 2551 239 324 1135 681 191 1471
Fragments arranged by size:
2551 1471 1135 681 324 239 219 191
SfoI GGC'GCC
Cuts at: 2144 2144
Size: 6811
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
SgrAI Cr'CCGG_yG
Cuts at: 1700 2347 1700
Size: 647 6164
SmaI CCC'GGG
Cuts at: 1872 4138 1872
Size: 2266 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2317 2524 2785 3577 3953 4915
Size: 197 926 374 207 261 792 376 962
Cuts at: 4915 5816 6078 820
Size: 901 262 1553
Fragments arranged by size:
1553 962 926 901 792 376 374 262
261 207 197
SnaBI TAC'GTA
Cuts at: 493 4602 493
Size: 4109 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6811
SphI G_CATG'C
Cuts at: 1132 4169 1132
Size: 3037 3774
SspI AAT'ATT
Cuts at: 5 52 2777 3061 4735 5
Size: 47 2725 284 1674 2081
Fragments arranged by size:
2725 2081 1674 284 47
StuI AGG'CCT
Cuts at: 1517 2826 1517
Size: 1309 5502
StyI C'CwwG_G
Cuts at: 513 1065 2004 2476 4250 513
Size: 552 939 472 1774 3074
Fragments arranged by size:
3074 1774 939 552 472
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4838 4855 5008 5193 5638 325
Size: 1255 3258 17 153 185 445 1498
Fragments arranged by size:
3258 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3372 5057 6769
Size: 274 80 33 51 1443 1395 1685 1712
Cuts at: 6769 96
Size: 138
Fragments arranged by size:
1712 1685 1443 1395 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2410 2442 3933 3965 6771 6803 2410
Size: 32 1491 32 2806 32 2418
Fragments arranged by size:
2806 2418 1491 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6811
XbaI T'CTAG_A
Cuts at: 4147 4147
Size: 6811
XhoI C'TCGA_G
Cuts at: 1943 2317 1943
Size: 374 6437
XmaI C'CCGG_G
Cuts at: 1870 4136 1870
Size: 2266 4545
XmnI GAAnn'nnTTC
Cuts at: 3072 4940 3072
Size: 1868 4943
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3763 4619 276
Size: 53 83 186 936 2229 856 2468
Fragments arranged by size:
2468 2229 936 856 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PsrI RsrII SacI SacII SanDI SexAI SfiI
SrfI SwaI XcmI