Restriction map for
pFN21AE5739
(Circular) (Six-base) MAPSORT of: pFN21AE5739.seq Check: 3913 from: 1 to: 6532
pFN21AE5739 6532 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2048 4342 278
Size: 53 83 186 936 512 2294 2468
Fragments arranged by size:
2468 2294 936 512 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3875 1937
Size: 1938 4594
Acc65I G'GTAC_C
Cuts at: 3853 3853
Size: 6532
AclI AA'CG_TT
Cuts at: 1563 2488 4659 5032 1563
Size: 925 2171 373 3063
Fragments arranged by size:
3063 2171 925 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4595 5979 6247 1632
Size: 2963 1384 268 1917
Fragments arranged by size:
2963 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6335
AflIII A'CryG_T
Cuts at: 1203 5431 6408 1203
Size: 4228 977 1327
Fragments arranged by size:
4228 1327 977
AgeI A'CCGG_T
Cuts at: 5363 5363
Size: 6532
AhdI GACnn_n'nnGTC
Cuts at: 5261 5261
Size: 6532
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6532
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6532
AlwNI CAG_nnn'CTG
Cuts at: 4275 5847 4275
Size: 1572 4960
ApaLI G'TGCA_C
Cuts at: 4589 5745 4589
Size: 1156 5376
ApoI r'AATT_y
Cuts at: 1493 2503 2812 3027 3164 3264 3841 4067 4219
Size: 1010 309 215 137 100 577 226 152
Cuts at: 4219 6328 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1010 577 309 226 215 152
137 100
AseI AT'TA_AT
Cuts at: 160 1032 5086 160
Size: 872 4054 1606
Fragments arranged by size:
4054 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
AvaI C'yCGr_G
Cuts at: 1870 1943 3857 1870
Size: 73 1914 4545
Fragments arranged by size:
4545 1914 73
AvrII C'CTAG_G
Cuts at: 2498 2498
Size: 6532
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2340 2373 2340
Size: 33 6499
BamHI G'GATC_C
Cuts at: 3862 4229 3862
Size: 367 6165
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3853 5308 618
Size: 325 217 627 2066 1455 1842
Fragments arranged by size:
2066 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3572 3851 1391
Size: 2181 279 4072
Fragments arranged by size:
4072 2181 279
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5561
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4723 4757 1903
Size: 34 2786 34 3678
Fragments arranged by size:
3678 2786 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2387 4424 5634 2387
Size: 2037 1210 3285
Fragments arranged by size:
3285 2037 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6532
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5143 136
Size: 107 122 71 631 605 3471 1525
Fragments arranged by size:
3471 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6527 6527
Size: 6532
BlpI GC'TnA_GC
Cuts at: 3949 3949
Size: 6532
Bme1580I G_kGCm'C
Cuts at: 1790 3410 4593 5749 1790
Size: 1620 1183 1156 2573
Fragments arranged by size:
2573 1620 1183 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6532
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4263 5221 442
Size: 416 53 83 399 24 2846 958 1753
Fragments arranged by size:
2846 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2097 1056
Size: 1041 5491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2766 5192 1239
Size: 1527 2426 2579
Fragments arranged by size:
2579 2426 1527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4657 5522 5820 4657
Size: 865 298 5369
Fragments arranged by size:
5369 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3455 5195 882
Size: 617 1956 1740 2219
Fragments arranged by size:
2219 1956 1740 617
BsaAI yAC'GTr
Cuts at: 493 1204 4323 493
Size: 711 3119 2702
Fragments arranged by size:
3119 2702 711
BsaBI GATnn'nnATC
Cuts at: 2475 6526 2475
Size: 4051 2481
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 4339 4721 5356
Size: 53 83 186 936 512 2294 382 635
Cuts at: 5356 275
Size: 1451
Fragments arranged by size:
2294 1451 936 635 512 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4965 5363 5637 5784 6195 4965
Size: 398 274 147 411 5302
Fragments arranged by size:
5302 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6307
Fragments arranged by size:
6307 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2205 2229 3710 1186
Size: 1019 24 1481 4008
Fragments arranged by size:
4008 1481 1019 24
BseYI C'CCAG_C
Cuts at: 5735 6231 5735
Size: 496 6036
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5959
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3982 4743 5771 1540
Size: 275 186 1981 761 1028 2301
Fragments arranged by size:
2301 1981 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2209 4593 4678 5749 2209
Size: 2384 85 1071 2992
Fragments arranged by size:
2992 2384 1071 85
BsmI GAATG_Cn'
Cuts at: 4043 4136 4043
Size: 93 6439
BsmBI CGTCTCn'nnnn_
Cuts at: 2036 2036
Size: 6532
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2209 3410 3572 3851 4593 4678 5749
Size: 399 419 1201 162 279 742 85 1071
Cuts at: 5749 1391
Size: 2174
Fragments arranged by size:
2174 1201 1071 742 419 399 279 162
85
BspEI T'CCGG_A
Cuts at: 6195 6195
Size: 6532
BspHI T'CATG_A
Cuts at: 4421 6151 4421
Size: 1730 4802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 3887 844
Size: 1213 1830 3489
Fragments arranged by size:
3489 1830 1213
BsrBI CCG'CTC
Cuts at: 1394 1823 4419 1394
Size: 429 2596 3507
Fragments arranged by size:
3507 2596 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2935 5027 5201 65
Size: 1183 1687 2092 174 1396
Fragments arranged by size:
2092 1687 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5176 5363 1700
Size: 110 3366 187 2869
Fragments arranged by size:
3366 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3236 96
Size: 3140 3392
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6532
BssSI C'ACGA_G
Cuts at: 4592 5604 4592
Size: 1012 5520
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6532
BstBI TT'CG_AA
Cuts at: 5336 5336
Size: 6532
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6532
BstXI CCAn_nnnn'nTGG
Cuts at: 2201 3748 2201
Size: 1547 4985
BstYI r'GATC_y
Cuts at: 1972 2747 3862 4229 4614 4631 6083 6169 6181
Size: 775 1115 367 385 17 1452 86 12
Cuts at: 6181 6527 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1115 775 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3435 513
Size: 552 2370 3610
Fragments arranged by size:
3610 2370 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5793
Fragments arranged by size:
5793 683 56
BtsI GCAGTG_nn'
Cuts at: 2529 4048 4842 4862 2529
Size: 1519 794 20 4199
Fragments arranged by size:
4199 1519 794 20
ClaI AT'CG_AT
Cuts at: 1554 2851 1554
Size: 1297 5235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6497
DraI TTT'AAA
Cuts at: 3130 3836 3130
Size: 706 5826
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5539 809
Size: 507 4223 1802
Fragments arranged by size:
4223 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3979 4868 6497
Size: 54 1425 50 275 18 2149 889 1629
Cuts at: 6497 8
Size: 43
Fragments arranged by size:
2149 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3979 1537
Size: 275 2167 4090
Fragments arranged by size:
4090 2167 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2990 3560 4462 1353
Size: 1637 570 902 3423
Fragments arranged by size:
3423 1637 902 570
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5119 5493 5639 387
Size: 1064 138 3530 374 146 1280
Fragments arranged by size:
3530 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2766 4595 5192 5979 6247 1239
Size: 393 1134 1829 597 787 268 1524
Fragments arranged by size:
1829 1524 1134 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3841 4219 3841
Size: 378 6154
FspI TGC'GCA
Cuts at: 5038 5038
Size: 6532
HaeII r_GCGC'y
Cuts at: 1678 5679 1678
Size: 4001 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2385 2417 2783 2815 3751
Size: 32 135 32 813 32 366 32 936
Cuts at: 3751 3783 5173 5205 5247 5279 1373
Size: 32 1390 32 42 32 2626
Fragments arranged by size:
2626 1390 936 813 366 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3876 4122 669
Size: 988 281 1938 246 3079
Fragments arranged by size:
3079 1938 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3590 748
Size: 2842 3690
HpaI GTT'AAC
Cuts at: 4122 4122
Size: 6532
KpnI G_GTAC'C
Cuts at: 3857 3857
Size: 6532
MfeI C'AATT_G
Cuts at: 4131 4131
Size: 6532
MluI A'CGCG_T
Cuts at: 6408 6408
Size: 6532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2142 2243 2657 5646 5830 1305
Size: 837 101 414 2989 184 2007
Fragments arranged by size:
2989 2007 837 414 184 101
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4710
Fragments arranged by size:
4710 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2199 2388 2479 4490 4849 5008 518
Size: 648 1033 189 91 2011 359 159 2042
Fragments arranged by size:
2042 2011 1033 648 359 189 159 91
MspA1I CmG'CkG
Cuts at: 1606 3764 3948 4625 5773 6018 1606
Size: 2158 184 677 1148 245 2120
Fragments arranged by size:
2158 2120 1148 677 245 184
NcoI C'CATG_G
Cuts at: 513 1065 3435 513
Size: 552 2370 3610
Fragments arranged by size:
3610 2370 552
NdeI CA'TA_TG
Cuts at: 387 2369 3774 387
Size: 1982 1405 3145
Fragments arranged by size:
3145 1982 1405
NheI G'CTAG_C
Cuts at: 1052 2093 1052
Size: 1041 5491
NotI GC'GGCC_GC
Cuts at: 3979 3979
Size: 6532
NspI r_CATG'y
Cuts at: 1132 1229 3387 3890 5435 1132
Size: 97 2158 503 1545 2229
Fragments arranged by size:
2229 2158 1545 503 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6532
PciI A'CATG_T
Cuts at: 5431 5431
Size: 6532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6532
PmeI GTTT'AAAC
Cuts at: 3836 3836
Size: 6532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4601 4633 6114 6146 4601
Size: 32 1481 32 4987
Fragments arranged by size:
4987 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6532
PsiI TTA'TAA
Cuts at: 4102 4102
Size: 6532
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3313 3345 3313
Size: 32 6500
PstI C_TGCA'G
Cuts at: 830 2068 3884 830
Size: 1238 1816 3478
Fragments arranged by size:
3478 1816 1238
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6532
PvuII CAG'CTG
Cuts at: 3764 3764
Size: 6532
SalI G'TCGA_C
Cuts at: 1936 3874 1936
Size: 1938 4594
SapI GCTCTTCn'nnn_
Cuts at: 2990 3560 2990
Size: 570 5962
SbfI CC_TGCA'GG
Cuts at: 3884 3884
Size: 6532
ScaI AGT'ACT
Cuts at: 4780 6492 4780
Size: 1712 4820
SexAI A'CCwGG_T
Cuts at: 2360 2360
Size: 6532
SfcI C'TryA_G
Cuts at: 826 1045 2064 2997 3880 5015 5696 5887 826
Size: 219 1019 933 883 1135 681 191 1471
Fragments arranged by size:
1471 1135 1019 933 883 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6532
SmaI CCC'GGG
Cuts at: 1872 3859 1872
Size: 1987 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4636 5537 5799 820
Size: 197 926 2693 901 262 1553
Fragments arranged by size:
2693 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4323 493
Size: 3830 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6532
SphI G_CATG'C
Cuts at: 1132 3387 3890 1132
Size: 2255 503 3774
Fragments arranged by size:
3774 2255 503
SspI AAT'ATT
Cuts at: 5 52 3529 4456 5
Size: 47 3477 927 2081
Fragments arranged by size:
3477 2081 927 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6532
StyI C'CwwG_G
Cuts at: 513 1065 2498 3435 3971 513
Size: 552 1433 937 536 3074
Fragments arranged by size:
3074 1433 937 552 536
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3256 4559 4576 4729 4914 5359 325
Size: 1255 1676 1303 17 153 185 445 1498
Fragments arranged by size:
1676 1498 1303 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2877 3236 3366
Size: 274 80 33 51 1443 900 359 130
Cuts at: 3366 4778 6490 96
Size: 1412 1712 138
Fragments arranged by size:
1712 1443 1412 900 359 274 138 130
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6492 6524 6492
Size: 32 6500
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6532
XbaI T'CTAG_A
Cuts at: 3868 3868
Size: 6532
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6532
XmaI C'CCGG_G
Cuts at: 1870 3857 1870
Size: 1987 4545
XmnI GAAnn'nnTTC
Cuts at: 3592 4661 3592
Size: 1069 5463
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2046 4340 276
Size: 53 83 186 936 512 2294 2468
Fragments arranged by size:
2468 2294 936 512 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI XcmI