Restriction map for
pFN21AE5738
(Circular) (Six-base) MAPSORT of: pFN21AE5738.seq Check: 6729 from: 1 to: 6814
pFN21AE5738 6814 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4624 278
Size: 53 83 186 936 3088 2468
Fragments arranged by size:
3088 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4157 1937
Size: 2220 4594
Acc65I G'GTAC_C
Cuts at: 4135 4135
Size: 6814
AclI AA'CG_TT
Cuts at: 1563 4113 4941 5314 1563
Size: 2550 828 373 3063
Fragments arranged by size:
3063 2550 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2834 3927 4877 6261 6529 1632
Size: 1202 1093 950 1384 268 1917
Fragments arranged by size:
1917 1384 1202 1093 950 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6617
AflIII A'CryG_T
Cuts at: 1203 2763 5713 6690 1203
Size: 1560 2950 977 1327
Fragments arranged by size:
2950 1560 1327 977
AgeI A'CCGG_T
Cuts at: 2724 5645 2724
Size: 2921 3893
AhdI GACnn_n'nnGTC
Cuts at: 5543 5543
Size: 6814
AleI CACnn'nnGTG
Cuts at: 1166 3089 1166
Size: 1923 4891
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6814
AlwNI CAG_nnn'CTG
Cuts at: 4557 6129 4557
Size: 1572 5242
ApaLI G'TGCA_C
Cuts at: 4871 6027 4871
Size: 1156 5658
ApoI r'AATT_y
Cuts at: 1493 2861 3052 3240 3863 4123 4349 4501 6610
Size: 1368 191 188 623 260 226 152 2109
Cuts at: 6610 1493
Size: 1697
Fragments arranged by size:
2109 1697 1368 623 260 226 191 188
152
AseI AT'TA_AT
Cuts at: 160 1032 5368 160
Size: 872 4336 1606
Fragments arranged by size:
4336 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
AvaI C'yCGr_G
Cuts at: 1870 1943 2577 4139 1870
Size: 73 634 1562 4545
Fragments arranged by size:
4545 1562 634 73
BamHI G'GATC_C
Cuts at: 4144 4511 4144
Size: 367 6447
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4135 5590 618
Size: 325 217 627 2348 1455 1842
Fragments arranged by size:
2348 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4133 1391
Size: 2742 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2234 928
Size: 971 335 5508
Fragments arranged by size:
5508 971 335
BbvCI CC'TCA_GC
Cuts at: 3062 3374 3062
Size: 312 6502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5005 5039 1903
Size: 34 50 34 2984 34 3678
Fragments arranged by size:
3678 2984 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4706 5916 3584
Size: 1122 1210 4482
Fragments arranged by size:
4482 1210 1122
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6814
BfrBI ATG'CAT
Cuts at: 3070 3224 3070
Size: 154 6660
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5425 136
Size: 107 122 71 631 605 3753 1525
Fragments arranged by size:
3753 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3784 6809 3784
Size: 3025 3789
BlpI GC'TnA_GC
Cuts at: 4231 4231
Size: 6814
Bme1580I G_kGCm'C
Cuts at: 1790 4875 6031 1790
Size: 3085 1156 2573
Fragments arranged by size:
3085 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6814
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2047 3323 3573
Size: 416 53 83 399 24 630 1276 250
Cuts at: 3573 4545 5503 442
Size: 972 958 1753
Fragments arranged by size:
1753 1276 972 958 630 416 399 250
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6814
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3796 3796
Size: 6814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3534 3666 3943 5474 1239
Size: 2295 132 277 1531 2579
Fragments arranged by size:
2579 2295 1531 277 132
Bpu10I CC'TnA_GC
Cuts at: 3062 3374 3062
Size: 312 6502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4939 5804 6102 4939
Size: 865 298 5651
Fragments arranged by size:
5651 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3954 5477 882
Size: 617 2455 1523 2219
Fragments arranged by size:
2455 2219 1523 617
BsaAI yAC'GTr
Cuts at: 493 1204 2764 3628 4605 493
Size: 711 1560 864 977 2702
Fragments arranged by size:
2702 1560 977 864 711
BsaBI GATnn'nnATC
Cuts at: 3572 6808 3572
Size: 3236 3578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4621 5003 5638 275
Size: 53 83 186 936 3088 382 635 1451
Fragments arranged by size:
3088 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2724 5247 5645 5919 6066 6477 2724
Size: 2523 398 274 147 411 3061
Fragments arranged by size:
3061 2523 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6589
Fragments arranged by size:
6589 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 3249 4034 1186
Size: 910 1153 785 3966
Fragments arranged by size:
3966 1153 910 785
BseYI C'CCAG_C
Cuts at: 2702 2990 6017 6513 2702
Size: 288 3027 496 3003
Fragments arranged by size:
3027 3003 496 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2667 1163
Size: 573 931 5310
Fragments arranged by size:
5310 931 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4264 5025 6053 1540
Size: 275 186 2263 761 1028 2301
Fragments arranged by size:
2301 2263 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3627 4875 4960 6031 3627
Size: 1248 85 1071 4410
Fragments arranged by size:
4410 1248 1071 85
BsmI GAATG_Cn'
Cuts at: 2427 3563 4325 4418 2427
Size: 1136 762 93 4823
Fragments arranged by size:
4823 1136 762 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3627 4133 4875 4960 6031 1391
Size: 399 1837 506 742 85 1071 2174
Fragments arranged by size:
2174 1837 1071 742 506 399 85
BspEI T'CCGG_A
Cuts at: 6477 6477
Size: 6814
BspHI T'CATG_A
Cuts at: 2437 4703 6433 2437
Size: 2266 1730 2818
Fragments arranged by size:
2818 2266 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4169 844
Size: 3325 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4701 1394
Size: 429 2878 3507
Fragments arranged by size:
3507 2878 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2292 2752 2985 3320 5309 5483 65
Size: 1183 1044 460 233 335 1989 174 1396
Fragments arranged by size:
1989 1396 1183 1044 460 335 233 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2724 5458 5645 1700
Size: 110 914 2734 187 2869
Fragments arranged by size:
2869 2734 914 187 110
BsrGI T'GTAC_A
Cuts at: 96 2901 96
Size: 2805 4009
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6814
BssSI C'ACGA_G
Cuts at: 4874 5886 4874
Size: 1012 5802
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6814
BstBI TT'CG_AA
Cuts at: 5618 5618
Size: 6814
BstEII G'GTnAC_C
Cuts at: 1184 2399 1184
Size: 1215 5599
BstYI r'GATC_y
Cuts at: 1972 2471 3784 4144 4511 4896 4913 6365 6451
Size: 499 1313 360 367 385 17 1452 86
Cuts at: 6451 6463 6809 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1313 499 385 367 360 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2772 3474 513
Size: 552 1707 702 3853
Fragments arranged by size:
3853 1707 702 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6075
Fragments arranged by size:
6075 683 56
BtsI GCAGTG_nn'
Cuts at: 2283 2425 2877 3162 3979 4330 5124 5144 2283
Size: 142 452 285 817 351 794 20 3953
Fragments arranged by size:
3953 817 794 452 351 285 142 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6814
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6779
DraI TTT'AAA
Cuts at: 3907 4118 3907
Size: 211 6603
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5821 809
Size: 507 4505 1802
Fragments arranged by size:
4505 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4261 5150 6779
Size: 54 1425 50 275 18 2431 889 1629
Cuts at: 6779 8
Size: 43
Fragments arranged by size:
2431 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4261 1537
Size: 275 2449 4090
Fragments arranged by size:
4090 2449 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2266 2810 3480 4011 4744 1353
Size: 913 544 670 531 733 3423
Fragments arranged by size:
3423 913 733 670 544 531
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5401 5775 5921 387
Size: 1064 138 3812 374 146 1280
Fragments arranged by size:
3812 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2834 3534 3666 3927 3943 4877 5474
Size: 393 1202 700 132 261 16 934 597
Cuts at: 5474 6261 6529 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1202 934 787 700 597 393 268
261 132 16
EcoNI CCTnn'n_nnAGG
Cuts at: 3043 3043
Size: 6814
EcoRI G'AATT_C
Cuts at: 4123 4501 4123
Size: 378 6436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3867 3867
Size: 6814
FspI TGC'GCA
Cuts at: 5320 5320
Size: 6814
HaeII r_GCGC'y
Cuts at: 1678 5961 1678
Size: 4283 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2560 2592 3009 3041 3434
Size: 32 135 32 988 32 417 32 393
Cuts at: 3434 3466 3577 3609 5455 5487 5529 5561 1373
Size: 32 111 32 1846 32 42 32 2626
Fragments arranged by size:
2626 1846 988 417 393 135 111 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3664 4158 4404 669
Size: 988 281 1726 494 246 3079
Fragments arranged by size:
3079 1726 988 494 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6814
HpaI GTT'AAC
Cuts at: 4404 4404
Size: 6814
KpnI G_GTAC'C
Cuts at: 4139 4139
Size: 6814
MfeI C'AATT_G
Cuts at: 4413 4413
Size: 6814
MluI A'CGCG_T
Cuts at: 6690 6690
Size: 6814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2107 5928 6112 1305
Size: 802 3821 184 2007
Fragments arranged by size:
3821 2007 802 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4992
Fragments arranged by size:
4992 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2514 2940 3075 3086 3089 3566 4772
Size: 648 1348 426 135 11 3 477 1206
Cuts at: 4772 5131 5290 518
Size: 359 159 2042
Fragments arranged by size:
2042 1348 1206 648 477 426 359 159
135 11 3
MspA1I CmG'CkG
Cuts at: 1606 3169 3554 3703 4230 4907 6055 6300 1606
Size: 1563 385 149 527 677 1148 245 2120
Fragments arranged by size:
2120 1563 1148 677 527 385 245 149
NcoI C'CATG_G
Cuts at: 513 1065 3474 513
Size: 552 2409 3853
Fragments arranged by size:
3853 2409 552
NdeI CA'TA_TG
Cuts at: 387 2808 387
Size: 2421 4393
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6814
NotI GC'GGCC_GC
Cuts at: 4261 4261
Size: 6814
NsiI A_TGCA'T
Cuts at: 3072 3226 3072
Size: 154 6660
NspI r_CATG'y
Cuts at: 1132 1229 2418 2897 3827 4172 5717 1132
Size: 97 1189 479 930 345 1545 2229
Fragments arranged by size:
2229 1545 1189 930 479 345 97
PasI CC'CwG_GG
Cuts at: 1348 2038 3537 1348
Size: 690 1499 4625
Fragments arranged by size:
4625 1499 690
PciI A'CATG_T
Cuts at: 5713 5713
Size: 6814
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6814
PmeI GTTT'AAAC
Cuts at: 4118 4118
Size: 6814
PmlI CAC'GTG
Cuts at: 2764 3628 2764
Size: 864 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2733 2765 4883 4915 6396 6428 2733
Size: 32 2118 32 1481 32 3119
Fragments arranged by size:
3119 2118 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6814
PsiI TTA'TAA
Cuts at: 4384 4384
Size: 6814
PstI C_TGCA'G
Cuts at: 830 4166 830
Size: 3336 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6814
PvuII CAG'CTG
Cuts at: 3169 3554 3703 3169
Size: 385 149 6280
Fragments arranged by size:
6280 385 149
SalI G'TCGA_C
Cuts at: 1936 4156 1936
Size: 2220 4594
SapI GCTCTTCn'nnn_
Cuts at: 2266 2266
Size: 6814
SbfI CC_TGCA'GG
Cuts at: 4166 4166
Size: 6814
ScaI AGT'ACT
Cuts at: 5062 6774 5062
Size: 1712 5102
SfcI C'TryA_G
Cuts at: 826 1045 4162 5297 5978 6169 826
Size: 219 3117 1135 681 191 1471
Fragments arranged by size:
3117 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6814
SmaI CCC'GGG
Cuts at: 1872 4141 1872
Size: 2269 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2577 4918 5819 6081 820
Size: 197 926 634 2341 901 262 1553
Fragments arranged by size:
2341 1553 926 901 634 262 197
SnaBI TAC'GTA
Cuts at: 493 4605 493
Size: 4112 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6814
SphI G_CATG'C
Cuts at: 1132 2418 3827 4172 1132
Size: 1286 1409 345 3774
Fragments arranged by size:
3774 1409 1286 345
SspI AAT'ATT
Cuts at: 5 52 2607 2656 4738 5
Size: 47 2555 49 2082 2081
Fragments arranged by size:
2555 2082 2081 49 47
StuI AGG'CCT
Cuts at: 1517 3919 3956 1517
Size: 2402 37 4375
Fragments arranged by size:
4375 2402 37
StyI C'CwwG_G
Cuts at: 513 1065 3474 4253 513
Size: 552 2409 779 3074
Fragments arranged by size:
3074 2409 779 552
SwaI ATTT'AAAT
Cuts at: 3907 3907
Size: 6814
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 2457 3453 4841 4858 5011 5196
Size: 1255 450 427 996 1388 17 153 185
Cuts at: 5196 5641 325
Size: 445 1498
Fragments arranged by size:
1498 1388 1255 996 450 445 427 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2901 5060 6772
Size: 274 80 33 51 1443 924 2159 1712
Cuts at: 6772 96
Size: 138
Fragments arranged by size:
2159 1712 1443 924 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3141 3173 6774 6806 3141
Size: 32 3601 32 3149
Fragments arranged by size:
3601 3149 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6814
XbaI T'CTAG_A
Cuts at: 4150 4150
Size: 6814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2312 4076 2312
Size: 1764 5050
XhoI C'TCGA_G
Cuts at: 1943 2577 1943
Size: 634 6180
XmaI C'CCGG_G
Cuts at: 1870 4139 1870
Size: 2269 4545
XmnI GAAnn'nnTTC
Cuts at: 2070 4943 2070
Size: 2873 3941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4622 276
Size: 53 83 186 936 3088 2468
Fragments arranged by size:
3088 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI