Restriction map for
pFN21AE5730
(Circular) (Six-base) MAPSORT of: pFN21AE5730.seq Check: 2726 from: 1 to: 6595
pFN21AE5730 6595 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2963 3528 2963
Size: 565 6030
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2714 4405 278
Size: 53 83 186 936 1178 1691 2468
Fragments arranged by size:
2468 1691 1178 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3938 1937
Size: 2001 4594
Acc65I G'GTAC_C
Cuts at: 3916 3916
Size: 6595
AclI AA'CG_TT
Cuts at: 1563 4722 5095 1563
Size: 3159 373 3063
Fragments arranged by size:
3159 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3470 4658 6042 6310 1632
Size: 1838 1188 1384 268 1917
Fragments arranged by size:
1917 1838 1384 1188 268
AfeI AGC'GCT
Cuts at: 2798 2798
Size: 6595
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6398
AflIII A'CryG_T
Cuts at: 1203 2253 3346 5494 6471 1203
Size: 1050 1093 2148 977 1327
Fragments arranged by size:
2148 1327 1093 1050 977
AgeI A'CCGG_T
Cuts at: 3603 5426 3603
Size: 1823 4772
AhdI GACnn_n'nnGTC
Cuts at: 5324 5324
Size: 6595
AleI CACnn'nnGTG
Cuts at: 1166 2519 1166
Size: 1353 5242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2558 3578 3839 1184
Size: 1374 1020 261 3940
Fragments arranged by size:
3940 1374 1020 261
AlwNI CAG_nnn'CTG
Cuts at: 2077 3431 4338 5910 2077
Size: 1354 907 1572 2762
Fragments arranged by size:
2762 1572 1354 907
ApaI G_GGCC'C
Cuts at: 2164 2164
Size: 6595
ApaLI G'TGCA_C
Cuts at: 3007 4652 5808 3007
Size: 1645 1156 3794
Fragments arranged by size:
3794 1645 1156
ApoI r'AATT_y
Cuts at: 1493 3120 3904 4130 4282 6391 1493
Size: 1627 784 226 152 2109 1697
Fragments arranged by size:
2109 1697 1627 784 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2607 5149 160
Size: 872 1575 2542 1606
Fragments arranged by size:
2542 1606 1575 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
AvaI C'yCGr_G
Cuts at: 1870 1943 3543 3566 3920 1870
Size: 73 1600 23 354 4545
Fragments arranged by size:
4545 1600 354 73 23
BamHI G'GATC_C
Cuts at: 3925 4292 3925
Size: 367 6228
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2330 2951 3301 3628 3686
Size: 325 217 627 543 621 350 327 58
Cuts at: 3686 3916 5371 618
Size: 230 1455 1842
Fragments arranged by size:
1842 1455 627 621 543 350 327 325
230 217 58
BanII G_rGCy'C
Cuts at: 1391 2164 2478 2844 3081 3914 1391
Size: 773 314 366 237 833 4072
Fragments arranged by size:
4072 833 773 366 314 237
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2222 3301 928
Size: 971 323 1079 4222
Fragments arranged by size:
4222 1079 971 323
BbvCI CC'TCA_GC
Cuts at: 2345 2345
Size: 6595
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4786 4820 1903
Size: 34 2849 34 3678
Fragments arranged by size:
3678 2849 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4487 5697 4487
Size: 1210 5385
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6595
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2949 3143 5206
Size: 107 122 71 631 605 1277 194 2063
Cuts at: 5206 136
Size: 1525
Fragments arranged by size:
2063 1525 1277 631 605 194 122 107
71
BglII A'GATC_T
Cuts at: 6590 6590
Size: 6595
BlpI GC'TnA_GC
Cuts at: 2843 4012 2843
Size: 1169 5426
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2164 2863 3011 3306 3469 3633 4656
Size: 271 103 699 148 295 163 164 1023
Cuts at: 4656 5812 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1023 699 295 271 164 163
148 103
BmgBI CAC'GTC
Cuts at: 1318 2560 1318
Size: 1242 5353
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2236 4326 5284
Size: 416 53 83 399 24 819 2090 958
Cuts at: 5284 442
Size: 1753
Fragments arranged by size:
2090 1753 958 819 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2275 2849 3251 3357 5255 1239
Size: 983 53 574 402 106 1898 2579
Fragments arranged by size:
2579 1898 983 574 402 106 53
Bpu10I CC'TnA_GC
Cuts at: 2345 2553 2345
Size: 208 6387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3008 4720 5585 5883 3008
Size: 1712 865 298 3720
Fragments arranged by size:
3720 1712 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2174 3704 3801 5258 882
Size: 617 675 1530 97 1457 2219
Fragments arranged by size:
2219 1530 1457 675 617 97
BsaAI yAC'GTr
Cuts at: 493 1204 2085 4386 493
Size: 711 881 2301 2702
Fragments arranged by size:
2702 2301 881 711
BsaBI GATnn'nnATC
Cuts at: 6589 6589
Size: 6595
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2711 4402 4784 5419
Size: 53 83 186 936 1178 1691 382 635
Cuts at: 5419 275
Size: 1451
Fragments arranged by size:
1691 1451 1178 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3603 5028 5426 5700 5847 6258 3603
Size: 1425 398 274 147 411 3940
Fragments arranged by size:
3940 1425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2390 2420 2853 2883 3122
Size: 30 165 30 987 30 433 30 239
Cuts at: 3122 3152 1178
Size: 30 4621
Fragments arranged by size:
4621 987 433 239 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2132 2473 2737 3130 1186
Size: 859 87 341 264 393 4651
Fragments arranged by size:
4651 859 393 341 264 87
BseYI C'CCAG_C
Cuts at: 2021 3702 3875 5798 6294 2021
Size: 1681 173 1923 496 2322
Fragments arranged by size:
2322 1923 1681 496 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 3023 1163
Size: 573 432 855 4735
Fragments arranged by size:
4735 855 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4045 4806 5834 1540
Size: 275 186 2044 761 1028 2301
Fragments arranged by size:
2301 2044 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2559 2844 3011 3081 4656 4741 5812 2559
Size: 285 167 70 1575 85 1071 3342
Fragments arranged by size:
3342 1575 1071 285 167 85 70
BsmI GAATG_Cn'
Cuts at: 4106 4199 4106
Size: 93 6502
BsmBI CGTCTCn'nnnn_
Cuts at: 2624 2940 2624
Size: 316 6279
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2164 2478 2559 2844 2863 3011
Size: 399 271 103 314 81 285 19 148
Cuts at: 3011 3081 3306 3469 3633 3914 4656 4741 5812
Size: 70 225 163 164 281 742 85 1071
Cuts at: 5812 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 314 285 281 271
225 164 163 148 103 85 81 70
19
BspEI T'CCGG_A
Cuts at: 6258 6258
Size: 6595
BspHI T'CATG_A
Cuts at: 4484 6214 4484
Size: 1730 4865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 2963 3528 3950 844
Size: 1253 866 565 422 3489
Fragments arranged by size:
3489 1253 866 565 422
BsrBI CCG'CTC
Cuts at: 1394 1823 2206 4482 1394
Size: 429 383 2276 3507
Fragments arranged by size:
3507 2276 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 5090 5264 65
Size: 1183 3842 174 1396
Fragments arranged by size:
3842 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2490 3603 5239 5426 1700
Size: 110 680 1113 1636 187 2869
Fragments arranged by size:
2869 1636 1113 680 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6595
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6595
BssSI C'ACGA_G
Cuts at: 2753 3868 4655 5667 2753
Size: 1115 787 1012 3681
Fragments arranged by size:
3681 1115 1012 787
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2098 794
Size: 1304 5291
BstBI TT'CG_AA
Cuts at: 5399 5399
Size: 6595
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6595
BstYI r'GATC_y
Cuts at: 1972 2632 3925 4292 4677 4694 6146 6232 6244
Size: 660 1293 367 385 17 1452 86 12
Cuts at: 6244 6590 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1293 660 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3639 513
Size: 552 939 1635 3469
Fragments arranged by size:
3469 1635 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5856
Fragments arranged by size:
5856 683 56
BtsI GCAGTG_nn'
Cuts at: 2032 2099 3011 3527 4111 4905 4925 2032
Size: 67 912 516 584 794 20 3702
Fragments arranged by size:
3702 912 794 584 516 67 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6595
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3762 3797 529
Size: 35 3198 35 3327
Fragments arranged by size:
3327 3198 35 35
DraI TTT'AAA
Cuts at: 3899 3899
Size: 6595
DraIII CAC_nnn'GTG
Cuts at: 2110 2110
Size: 6595
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3185 5602 809
Size: 507 1869 2417 1802
Fragments arranged by size:
2417 1869 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2500 3366
Size: 54 1425 50 275 18 249 421 866
Cuts at: 3366 3489 3504 4042 4931 6560 8
Size: 123 15 538 889 1629 43
Fragments arranged by size:
1629 1425 889 866 538 421 275 249
123 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 4042 1537
Size: 275 2230 4090
Fragments arranged by size:
4090 2230 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 2483 4525 1353
Size: 773 357 2042 3423
Fragments arranged by size:
3423 2042 773 357
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2603 2895 3317 5182 5556 5702
Size: 1064 138 1014 292 422 1865 374 146
Cuts at: 5702 387
Size: 1280
Fragments arranged by size:
1865 1280 1064 1014 422 374 292 146
138
EcoICRI GAG'CTC
Cuts at: 2842 3079 2842
Size: 237 6358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2275 2849 3251 3357 3470 4658
Size: 393 590 53 574 402 106 113 1188
Cuts at: 4658 5255 6042 6310 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1188 787 597 590 574 402 393
268 113 106 53
EcoNI CCTnn'n_nnAGG
Cuts at: 3280 3280
Size: 6595
EcoO109I rG'GnC_Cy
Cuts at: 2160 2161 2389 2160
Size: 1 228 6366
Fragments arranged by size:
6366 228 1
EcoRI G'AATT_C
Cuts at: 3904 4282 3904
Size: 378 6217
FspI TGC'GCA
Cuts at: 5101 5101
Size: 6595
HaeII r_GCGC'y
Cuts at: 1678 2800 5742 1678
Size: 1122 2942 2531
Fragments arranged by size:
2942 2531 1122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2296 2328 2390 2422 3305
Size: 32 135 32 724 32 62 32 883
Cuts at: 3305 3337 5236 5268 5310 5342 1373
Size: 32 1899 32 42 32 2626
Fragments arranged by size:
2626 1899 883 724 135 62 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 3939 4185 669
Size: 988 281 349 1652 246 3079
Fragments arranged by size:
3079 1652 988 349 281 246
HindIII A'AGCT_T
Cuts at: 748 3437 748
Size: 2689 3906
HpaI GTT'AAC
Cuts at: 4185 4185
Size: 6595
KpnI G_GTAC'C
Cuts at: 3920 3920
Size: 6595
MfeI C'AATT_G
Cuts at: 2697 4194 2697
Size: 1497 5098
MluI A'CGCG_T
Cuts at: 3346 6471 3346
Size: 3125 3470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2697 5709 5893 1305
Size: 1392 3012 184 2007
Fragments arranged by size:
3012 2007 1392 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 3491 3506 10
Size: 54 1425 343 249 1410 15 3099
Fragments arranged by size:
3099 1425 1410 343 249 54 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2519 3257 3485 3644 3800 4553 4912
Size: 648 1353 738 228 159 156 753 359
Cuts at: 4912 5071 518
Size: 159 2042
Fragments arranged by size:
2042 1353 753 738 648 359 228 159
159 156
MspA1I CmG'CkG
Cuts at: 1606 2065 2344 2437 3271 3744 4011 4688 5836
Size: 459 279 93 834 473 267 677 1148
Cuts at: 5836 6081 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 834 677 473 459 279 267
245 93
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5104
Fragments arranged by size:
5104 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6595
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6595
NotI GC'GGCC_GC
Cuts at: 4042 4042
Size: 6595
NspI r_CATG'y
Cuts at: 1132 1229 2101 2257 3953 5498 1132
Size: 97 872 156 1696 1545 2229
Fragments arranged by size:
2229 1696 1545 872 156 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6595
PciI A'CATG_T
Cuts at: 2253 5494 2253
Size: 3241 3354
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2910 2987 3523 1108
Size: 1802 77 536 4180
Fragments arranged by size:
4180 1802 536 77
PfoI T'CCnGG_A
Cuts at: 2199 3336 2199
Size: 1137 5458
PmeI GTTT'AAAC
Cuts at: 3899 3899
Size: 6595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2140 4664 4696 6177 6209 2108
Size: 32 2524 32 1481 32 2494
Fragments arranged by size:
2524 2494 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6595
PsiI TTA'TAA
Cuts at: 4165 4165
Size: 6595
PspOMI G'GGCC_C
Cuts at: 2160 2160
Size: 6595
PstI C_TGCA'G
Cuts at: 830 2070 2094 2190 2931 3818 3947 830
Size: 1240 24 96 741 887 129 3478
Fragments arranged by size:
3478 1240 887 741 129 96 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6595
PvuII CAG'CTG
Cuts at: 2344 2437 3271 3744 2344
Size: 93 834 473 5195
Fragments arranged by size:
5195 834 473 93
SacI G_AGCT'C
Cuts at: 2844 3081 2844
Size: 237 6358
SalI G'TCGA_C
Cuts at: 1936 3937 1936
Size: 2001 4594
SapI GCTCTTCn'nnn_
Cuts at: 2126 2483 2126
Size: 357 6238
SbfI CC_TGCA'GG
Cuts at: 3947 3947
Size: 6595
ScaI AGT'ACT
Cuts at: 2240 4843 6555 2240
Size: 2603 1712 2280
Fragments arranged by size:
2603 2280 1712
SfcI C'TryA_G
Cuts at: 826 1045 2066 2090 2186 2887 2927 3280 3814
Size: 219 1021 24 96 701 40 353 534
Cuts at: 3814 3943 5078 5759 5950 826
Size: 129 1135 681 191 1471
Fragments arranged by size:
1471 1135 1021 701 681 534 353 219
191 129 96 40 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6595
SmaI CCC'GGG
Cuts at: 1872 3545 3922 1872
Size: 1673 377 4545
Fragments arranged by size:
4545 1673 377
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3023 4699 5600 5862 820
Size: 197 926 1080 1676 901 262 1553
Fragments arranged by size:
1676 1553 1080 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4386 493
Size: 3893 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6595
SphI G_CATG'C
Cuts at: 1132 2101 3953 1132
Size: 969 1852 3774
Fragments arranged by size:
3774 1852 969
SrfI GCCC'GGGC
Cuts at: 3545 3545
Size: 6595
SspI AAT'ATT
Cuts at: 5 52 4519 5
Size: 47 4467 2081
Fragments arranged by size:
4467 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6595
StyI C'CwwG_G
Cuts at: 513 1065 2004 4034 513
Size: 552 939 2030 3074
Fragments arranged by size:
3074 2030 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2218 2867 4622 4639 4792 4977 5422
Size: 1255 638 649 1755 17 153 185 445
Cuts at: 5422 325
Size: 1498
Fragments arranged by size:
1755 1498 1255 649 638 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2238 2374 4841
Size: 274 80 33 51 1443 261 136 2467
Cuts at: 4841 6553 96
Size: 1712 138
Fragments arranged by size:
2467 1712 1443 274 261 138 136 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2853 2885 3122 3154 6555 6587 2853
Size: 32 237 32 3401 32 2861
Fragments arranged by size:
3401 2861 237 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3068 1316
Size: 1752 4843
XbaI T'CTAG_A
Cuts at: 3931 3931
Size: 6595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3499 3499
Size: 6595
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6595
XmaI C'CCGG_G
Cuts at: 1870 3543 3920 1870
Size: 1673 377 4545
Fragments arranged by size:
4545 1673 377
XmnI GAAnn'nnTTC
Cuts at: 3153 3215 4724 3153
Size: 62 1509 5024
Fragments arranged by size:
5024 1509 62
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2712 4403 276
Size: 53 83 186 936 1178 1691 2468
Fragments arranged by size:
2468 1691 1178 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SwaI