Restriction map for
pFN21AE5727
(Circular) (Six-base) MAPSORT of: pFN21AE5727.seq Check: 7054 from: 1 to: 6826
pFN21AE5727 6826 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2478 3375 2478
Size: 897 5929
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2307 4068 4636 278
Size: 53 83 186 936 771 1761 568 2468
Fragments arranged by size:
2468 1761 936 771 568 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3396 4169 1937
Size: 1459 773 4594
Fragments arranged by size:
4594 1459 773
Acc65I G'GTAC_C
Cuts at: 2090 4147 2090
Size: 2057 4769
AclI AA'CG_TT
Cuts at: 1563 4953 5326 1563
Size: 3390 373 3063
Fragments arranged by size:
3390 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3484 3494 4889 6273 6541 1632
Size: 1852 10 1395 1384 268 1917
Fragments arranged by size:
1917 1852 1395 1384 268 10
AfeI AGC'GCT
Cuts at: 2411 3302 2411
Size: 891 5935
AflII C'TTAA_G
Cuts at: 820 1017 2459 820
Size: 197 1442 5187
Fragments arranged by size:
5187 1442 197
AflIII A'CryG_T
Cuts at: 1203 3946 5725 6702 1203
Size: 2743 1779 977 1327
Fragments arranged by size:
2743 1779 1327 977
AgeI A'CCGG_T
Cuts at: 2080 5657 2080
Size: 3577 3249
AhdI GACnn_n'nnGTC
Cuts at: 5555 5555
Size: 6826
AleI CACnn'nnGTG
Cuts at: 1166 2036 3808 1166
Size: 870 1772 4184
Fragments arranged by size:
4184 1772 870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3383 1184
Size: 2199 4627
AlwNI CAG_nnn'CTG
Cuts at: 2248 2558 3589 3976 4569 6141 2248
Size: 310 1031 387 593 1572 2933
Fragments arranged by size:
2933 1572 1031 593 387 310
ApaI G_GGCC'C
Cuts at: 2648 3160 2648
Size: 512 6314
ApaLI G'TGCA_C
Cuts at: 4883 6039 4883
Size: 1156 5670
ApoI r'AATT_y
Cuts at: 1493 4135 4361 4513 6622 1493
Size: 2642 226 152 2109 1697
Fragments arranged by size:
2642 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5380 160
Size: 872 4348 1606
Fragments arranged by size:
4348 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
AvaI C'yCGr_G
Cuts at: 1870 1943 2132 2799 3020 3296 3325 3631 4000
Size: 73 189 667 221 276 29 306 369
Cuts at: 4000 4094 4151 1870
Size: 94 57 4545
Fragments arranged by size:
4545 667 369 306 276 221 189 94
73 57 29
BamHI G'GATC_C
Cuts at: 4036 4156 4523 4036
Size: 120 367 6339
Fragments arranged by size:
6339 367 120
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2083 2090 2507 2543 2590
Size: 325 217 627 296 7 417 36 47
Cuts at: 2590 3015 3668 4004 4147 5602 618
Size: 425 653 336 143 1455 1842
Fragments arranged by size:
1842 1455 653 627 425 417 336 325
296 217 143 47 36 7
BanII G_rGCy'C
Cuts at: 1391 2133 2192 2648 3160 3768 4014 4145 1391
Size: 742 59 456 512 608 246 131 4072
Fragments arranged by size:
4072 742 608 512 456 246 131 59
BbeI G_GCGC'C
Cuts at: 2511 2547 2594 3019 2511
Size: 36 47 425 6318
Fragments arranged by size:
6318 425 47 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5855
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3584 3618 5017 5051 1903
Size: 34 50 34 1563 34 1399 34 3678
Fragments arranged by size:
3678 1563 1399 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3489 4718 5928 3489
Size: 1229 1210 4387
Fragments arranged by size:
4387 1229 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6826
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3656 3966 5437
Size: 107 122 71 631 605 1984 310 1471
Cuts at: 5437 136
Size: 1525
Fragments arranged by size:
1984 1525 1471 631 605 310 122 107
71
BglII A'GATC_T
Cuts at: 2280 6821 2280
Size: 4541 2285
BlpI GC'TnA_GC
Cuts at: 2881 4243 2881
Size: 1362 5464
Bme1580I G_kGCm'C
Cuts at: 1790 2648 2779 2814 3160 4887 6043 1790
Size: 858 131 35 346 1727 1156 2573
Fragments arranged by size:
2573 1727 1156 858 346 131 35
BmgBI CAC'GTC
Cuts at: 1318 4060 1318
Size: 2742 4084
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4557 5515 442
Size: 416 53 83 399 24 3140 958 1753
Fragments arranged by size:
3140 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6826
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2990 3508 2990
Size: 518 6308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2452 3959 5486 1239
Size: 980 233 1507 1527 2579
Fragments arranged by size:
2579 1527 1507 980 233
Bpu10I CC'TnA_GC
Cuts at: 3524 3524
Size: 6826
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3752 3791 4951 5816 6114 3752
Size: 39 1160 865 298 4464
Fragments arranged by size:
4464 1160 865 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2576 3338 5489 882
Size: 617 1077 762 2151 2219
Fragments arranged by size:
2219 2151 1077 762 617
BsaAI yAC'GTr
Cuts at: 493 1204 3217 4617 493
Size: 711 2013 1400 2702
Fragments arranged by size:
2702 2013 1400 711
BsaBI GATnn'nnATC
Cuts at: 6820 6820
Size: 6826
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 2508 2544 2591
Size: 53 83 186 936 771 204 36 47
Cuts at: 2591 3016 3200 3622 4065 4633 5015 5650 275
Size: 425 184 422 443 568 382 635 1451
Fragments arranged by size:
1451 936 771 635 568 443 425 422
382 204 186 184 83 53 47 36
BsaWI w'CCGG_w
Cuts at: 2080 3481 5259 5657 5931 6078 6489 2080
Size: 1401 1778 398 274 147 411 2417
Fragments arranged by size:
2417 1778 1401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2365 2395 1178
Size: 30 165 30 962 30 5609
Fragments arranged by size:
5609 962 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 2420 2827 2960 1186
Size: 1147 87 407 133 5052
Fragments arranged by size:
5052 1147 407 133 87
BseYI C'CCAG_C
Cuts at: 2101 2551 2992 3110 3538 3842 6029 6525 2101
Size: 450 441 118 428 304 2187 496 2402
Fragments arranged by size:
2402 2187 496 450 441 428 304 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2501 3379 3854 1163
Size: 573 765 878 475 4135
Fragments arranged by size:
4135 878 765 573 475
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2064 2922 3519 3562 3651 4276
Size: 275 186 63 858 597 43 89 625
Cuts at: 4276 5037 6065 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 858 761 625 597 275 186
89 63 43
BsiHKAI G_wGCw'C
Cuts at: 4887 4972 6043 4887
Size: 85 1071 5670
Fragments arranged by size:
5670 1071 85
BsmI GAATG_Cn'
Cuts at: 4337 4430 4337
Size: 93 6733
BsmBI CGTCTCn'nnnn_
Cuts at: 2295 2295
Size: 6826
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2192 2648 2779 2814 3160 3768
Size: 399 343 59 456 131 35 346 608
Cuts at: 3768 4014 4145 4887 4972 6043 1391
Size: 246 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 608 456 399 346 343
246 131 131 85 59 35
BspEI T'CCGG_A
Cuts at: 3481 6489 3481
Size: 3008 3818
BspHI T'CATG_A
Cuts at: 4715 6445 4715
Size: 1730 5096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2478 3375 3692 4181 844
Size: 1634 897 317 489 3489
Fragments arranged by size:
3489 1634 897 489 317
BsrBI CCG'CTC
Cuts at: 1394 1823 2790 2924 3027 3448 4713 1394
Size: 429 967 134 103 421 1265 3507
Fragments arranged by size:
3507 1265 967 429 421 134 103
BsrDI GCAATG_nn'
Cuts at: 65 1248 2294 5321 5495 65
Size: 1183 1046 3027 174 1396
Fragments arranged by size:
3027 1396 1183 1046 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2063 2080 2897 2969 3382 3424 3646
Size: 110 253 17 817 72 413 42 222
Cuts at: 3646 3716 5470 5657 1700
Size: 70 1754 187 2869
Fragments arranged by size:
2869 1754 817 413 253 222 187 110
72 70 42 17
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6826
BssHII G'CGCG_C
Cuts at: 1925 3223 3306 1925
Size: 1298 83 5445
Fragments arranged by size:
5445 1298 83
BssSI C'ACGA_G
Cuts at: 4886 5898 4886
Size: 1012 5814
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6826
BstBI TT'CG_AA
Cuts at: 5630 5630
Size: 6826
BstEII G'GTnAC_C
Cuts at: 1184 3056 1184
Size: 1872 4954
BstXI CCAn_nnnn'nTGG
Cuts at: 2736 2736
Size: 6826
BstYI r'GATC_y
Cuts at: 1972 2280 3771 4036 4156 4523 4908 4925 6377
Size: 308 1491 265 120 367 385 17 1452
Cuts at: 6377 6463 6475 6821 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1491 1452 385 367 346 308 265
120 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6087
Fragments arranged by size:
6087 683 56
BtsI GCAGTG_nn'
Cuts at: 2479 4342 5136 5156 2479
Size: 1863 794 20 4149
Fragments arranged by size:
4149 1863 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6826
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6791
DraI TTT'AAA
Cuts at: 4130 4130
Size: 6826
DraIII CAC_nnn'GTG
Cuts at: 2485 2485
Size: 6826
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4064 5833 809
Size: 507 2748 1769 1802
Fragments arranged by size:
2748 1802 1769 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2712 2919
Size: 54 1425 50 275 18 231 651 207
Cuts at: 2919 3426 3516 3549 3648 3718 3967 4273 5162
Size: 507 90 33 99 70 249 306 889
Cuts at: 5162 6791 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 651 507 306 275 249
231 207 99 90 70 54 50 43
33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2061 2919 3516 3648 4273 1537
Size: 275 249 858 597 132 625 4090
Fragments arranged by size:
4090 858 625 597 275 249 132
EarI CTCTTCn'nnn_
Cuts at: 1353 2704 3189 4756 1353
Size: 1351 485 1567 3423
Fragments arranged by size:
3423 1567 1351 485
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3193 5413 5787 5933 387
Size: 1064 138 1604 2220 374 146 1280
Fragments arranged by size:
2220 1604 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2452 3484 3494 3959 4889 5486
Size: 393 587 233 1032 10 465 930 597
Cuts at: 5486 6273 6541 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1032 930 787 597 587 465 393
268 233 10
EcoO109I rG'GnC_Cy
Cuts at: 3115 3156 3157 3934 3115
Size: 41 1 777 6007
Fragments arranged by size:
6007 777 41 1
EcoRI G'AATT_C
Cuts at: 4135 4513 4135
Size: 378 6448
FspI TGC'GCA
Cuts at: 2153 3267 5332 2153
Size: 1114 2065 3647
Fragments arranged by size:
3647 2065 1114
FspAI rTGC'GCAy
Cuts at: 2153 2153
Size: 6826
HaeII r_GCGC'y
Cuts at: 1678 2027 2413 2511 2547 2594 3019 3304 5973
Size: 349 386 98 36 47 425 285 2669
Cuts at: 5973 1678
Size: 2531
Fragments arranged by size:
2669 2531 425 386 349 285 98 47
36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2265 2297 2371 2403 3527
Size: 32 135 32 693 32 74 32 1124
Cuts at: 3527 3559 5467 5499 5541 5573 1373
Size: 32 1908 32 42 32 2626
Fragments arranged by size:
2626 1908 1124 693 135 74 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4170 4416 669
Size: 988 281 2232 246 3079
Fragments arranged by size:
3079 2232 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6826
HpaI GTT'AAC
Cuts at: 4416 4416
Size: 6826
KasI G'GCGC_C
Cuts at: 2507 2543 2590 3015 2507
Size: 36 47 425 6318
Fragments arranged by size:
6318 425 47 36
KpnI G_GTAC'C
Cuts at: 2094 4151 2094
Size: 2057 4769
MfeI C'AATT_G
Cuts at: 4425 4425
Size: 6826
MluI A'CGCG_T
Cuts at: 6702 6702
Size: 6826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2408 5940 6124 1305
Size: 1103 3532 184 2007
Fragments arranged by size:
3532 2007 1103 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3551 3969 10
Size: 54 1425 343 1719 418 2867
Fragments arranged by size:
2867 1719 1425 418 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 3808 4784 5143 5302 518
Size: 648 870 1772 976 359 159 2042
Fragments arranged by size:
2042 1772 976 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2493 2555 2767 3271 3504 3515 3589 3687
Size: 887 62 212 504 233 11 74 98
Cuts at: 3687 3696 3897 4242 4919 6067 6312 1606
Size: 9 201 345 677 1148 245 2120
Fragments arranged by size:
2120 1148 887 677 504 345 245 233
212 201 98 74 62 11 9
NaeI GCC'GGC
Cuts at: 2065 2899 2971 3384 2065
Size: 834 72 413 5507
Fragments arranged by size:
5507 834 413 72
NarI GG'CG_CC
Cuts at: 2508 2544 2591 3016 2508
Size: 36 47 425 6318
Fragments arranged by size:
6318 425 47 36
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6274
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6826
NgoMIV G'CCGG_C
Cuts at: 2063 2897 2969 3382 2063
Size: 834 72 413 5507
Fragments arranged by size:
5507 834 413 72
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6826
NotI GC'GGCC_GC
Cuts at: 2919 3516 4273 2919
Size: 597 757 5472
Fragments arranged by size:
5472 757 597
NspI r_CATG'y
Cuts at: 1132 1229 3781 3950 4184 5729 1132
Size: 97 2552 169 234 1545 2229
Fragments arranged by size:
2552 2229 1545 234 169 97
PasI CC'CwG_GG
Cuts at: 1348 2641 1348
Size: 1293 5533
PciI A'CATG_T
Cuts at: 3946 5725 3946
Size: 1779 5047
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2641 1108
Size: 1533 5293
PmeI GTTT'AAAC
Cuts at: 4130 4130
Size: 6826
PmlI CAC'GTG
Cuts at: 3217 3217
Size: 6826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4895 4927 6408 6440 4895
Size: 32 1481 32 5281
Fragments arranged by size:
5281 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3115 3934 3115
Size: 819 6007
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6826
PsiI TTA'TAA
Cuts at: 4396 4396
Size: 6826
PspOMI G'GGCC_C
Cuts at: 2644 3156 2644
Size: 512 6314
PspXI vC'TCGA_Gb
Cuts at: 3296 3296
Size: 6826
PstI C_TGCA'G
Cuts at: 830 3514 4178 830
Size: 2684 664 3478
Fragments arranged by size:
3478 2684 664
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6826
PvuII CAG'CTG
Cuts at: 2767 3271 3589 2767
Size: 504 318 6004
Fragments arranged by size:
6004 504 318
RsrII CG'GwC_CG
Cuts at: 2078 2078
Size: 6826
SalI G'TCGA_C
Cuts at: 1936 4168 1936
Size: 2232 4594
SanDI GG'GwC_CC
Cuts at: 3115 3115
Size: 6826
SbfI CC_TGCA'GG
Cuts at: 4178 4178
Size: 6826
ScaI AGT'ACT
Cuts at: 5074 6786 5074
Size: 1712 5114
SfcI C'TryA_G
Cuts at: 826 1045 3510 4174 5309 5990 6181 826
Size: 219 2465 664 1135 681 191 1471
Fragments arranged by size:
2465 1471 1135 681 664 219 191
SfoI GGC'GCC
Cuts at: 2509 2545 2592 3017 2509
Size: 36 47 425 6318
Fragments arranged by size:
6318 425 47 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6826
SmaI CCC'GGG
Cuts at: 1872 2134 3022 3327 4002 4153 1872
Size: 262 888 305 675 151 4545
Fragments arranged by size:
4545 888 675 305 262 151
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2459 3296 3767 3806 4930 5831
Size: 197 926 516 837 471 39 1124 901
Cuts at: 5831 6093 820
Size: 262 1553
Fragments arranged by size:
1553 1124 926 901 837 516 471 262
197 39
SnaBI TAC'GTA
Cuts at: 493 4617 493
Size: 4124 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6826
SphI G_CATG'C
Cuts at: 1132 4184 1132
Size: 3052 3774
SrfI GCCC'GGGC
Cuts at: 2134 2134
Size: 6826
SspI AAT'ATT
Cuts at: 5 52 4750 5
Size: 47 4698 2081
Fragments arranged by size:
4698 2081 47
StuI AGG'CCT
Cuts at: 1517 2663 2696 1517
Size: 1146 33 5647
Fragments arranged by size:
5647 1146 33
StyI C'CwwG_G
Cuts at: 513 1065 2346 2539 4265 513
Size: 552 1281 193 1726 3074
Fragments arranged by size:
3074 1726 1281 552 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2558 3576 4853 4870 5023 5208 5653
Size: 1255 978 1018 1277 17 153 185 445
Cuts at: 5653 325
Size: 1498
Fragments arranged by size:
1498 1277 1255 1018 978 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3747 5072 6784
Size: 274 80 33 51 1443 1770 1325 1712
Cuts at: 6784 96
Size: 138
Fragments arranged by size:
1770 1712 1443 1325 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2365 2397 6786 6818 2365
Size: 32 4389 32 2373
Fragments arranged by size:
4389 2373 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6826
XbaI T'CTAG_A
Cuts at: 4162 4162
Size: 6826
XhoI C'TCGA_G
Cuts at: 1943 3296 1943
Size: 1353 5473
XmaI C'CCGG_G
Cuts at: 1870 2132 3020 3325 4000 4151 1870
Size: 262 888 305 675 151 4545
Fragments arranged by size:
4545 888 675 305 262 151
XmnI GAAnn'nnTTC
Cuts at: 4955 4955
Size: 6826
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2305 4066 4634 276
Size: 53 83 186 936 771 1761 568 2468
Fragments arranged by size:
2468 1761 936 771 568 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI NruI NsiI
PacI PfoI PsrI SacI SacII SapI SexAI SfiI
SwaI XcmI