(Circular) (Six-base) MAPSORT of: pFN21AE5726.seq Check: 6923 from: 1 to: 6838 pFN21AE5726 6838 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4648 278 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2832 3661 3740 4181 1937 Size: 895 829 79 441 4594 Fragments arranged by size: 4594 895 829 441 79 Acc65I G'GTAC_C Cuts at: 4159 4159 Size: 6838 AclI AA'CG_TT Cuts at: 1563 4965 5338 1563 Size: 3402 373 3063 Fragments arranged by size: 3402 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4901 6285 6553 1632 Size: 3269 1384 268 1917 Fragments arranged by size: 3269 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6641 AflIII A'CryG_T Cuts at: 1203 3494 5737 6714 1203 Size: 2291 2243 977 1327 Fragments arranged by size: 2291 2243 1327 977 AgeI A'CCGG_T Cuts at: 5669 5669 Size: 6838 AhdI GACnn_n'nnGTC Cuts at: 5567 5567 Size: 6838 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6838 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2719 3366 1184 Size: 1535 647 4656 Fragments arranged by size: 4656 1535 647 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4019 4051 4019 Size: 32 6806 AlwNI CAG_nnn'CTG Cuts at: 2442 4581 6153 2442 Size: 2139 1572 3127 Fragments arranged by size: 3127 2139 1572 ApaI G_GGCC'C Cuts at: 2341 2341 Size: 6838 ApaLI G'TGCA_C Cuts at: 4895 6051 4895 Size: 1156 5682 ApoI r'AATT_y Cuts at: 1493 2925 4147 4373 4525 6634 1493 Size: 1432 1222 226 152 2109 1697 Fragments arranged by size: 2109 1697 1432 1222 226 152 AseI AT'TA_AT Cuts at: 160 1032 3831 3969 5392 160 Size: 872 2799 138 1423 1606 Fragments arranged by size: 2799 1606 1423 872 138 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 AvaI C'yCGr_G Cuts at: 1870 1943 2334 4163 1870 Size: 73 391 1829 4545 Fragments arranged by size: 4545 1829 391 73 AvrII C'CTAG_G Cuts at: 3688 3688 Size: 6838 BamHI G'GATC_C Cuts at: 2450 2527 3312 4168 4535 2450 Size: 77 785 856 367 4753 Fragments arranged by size: 4753 856 785 367 77 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4159 5614 618 Size: 325 217 627 2372 1455 1842 Fragments arranged by size: 2372 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2202 2341 2364 4157 1391 Size: 811 139 23 1793 4072 Fragments arranged by size: 4072 1793 811 139 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2139 3000 3231 928 Size: 971 240 861 231 4535 Fragments arranged by size: 4535 971 861 240 231 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5029 5063 1903 Size: 34 3092 34 3678 Fragments arranged by size: 3678 3092 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4730 5940 4730 Size: 1210 5628 BclI T'GATC_A Cuts at: 1548 1548 Size: 6838 BfrBI ATG'CAT Cuts at: 2706 2706 Size: 6838 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5449 136 Size: 107 122 71 631 605 3777 1525 Fragments arranged by size: 3777 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6833 6833 Size: 6838 BlpI GC'TnA_GC Cuts at: 4255 4255 Size: 6838 Bme1580I G_kGCm'C Cuts at: 1790 2341 3936 4899 6055 1790 Size: 551 1595 963 1156 2573 Fragments arranged by size: 2573 1595 1156 963 551 BmgBI CAC'GTC Cuts at: 1318 2156 2223 3382 1318 Size: 838 67 1159 4774 Fragments arranged by size: 4774 1159 838 67 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4569 5527 442 Size: 416 53 83 399 24 3152 958 1753 Fragments arranged by size: 3152 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6838 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3291 3291 Size: 6838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2367 2576 5498 1239 Size: 959 169 209 2922 2579 Fragments arranged by size: 2922 2579 959 209 169 Bpu10I CC'TnA_GC Cuts at: 2539 2539 Size: 6838 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3776 4963 5828 6126 3776 Size: 1187 865 298 4488 Fragments arranged by size: 4488 1187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5501 882 Size: 617 4002 2219 Fragments arranged by size: 4002 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2716 4629 493 Size: 711 1512 1913 2702 Fragments arranged by size: 2702 1913 1512 711 BsaBI GATnn'nnATC Cuts at: 3311 6832 3311 Size: 3521 3317 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2144 4645 5027 5662 Size: 53 83 186 936 611 2501 382 635 Cuts at: 5662 275 Size: 1451 Fragments arranged by size: 2501 1451 936 635 611 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2060 5271 5669 5943 6090 6501 2060 Size: 3211 398 274 147 411 2397 Fragments arranged by size: 3211 2397 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 2549 2579 1178 Size: 30 165 30 867 30 249 30 5437 Fragments arranged by size: 5437 867 249 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2088 2102 2132 2180 2225 2278 3267 1186 Size: 902 14 30 48 45 53 989 4757 Fragments arranged by size: 4757 989 902 53 48 45 30 14 BseYI C'CCAG_C Cuts at: 2328 3212 3537 6041 6537 2328 Size: 884 325 2504 496 2629 Fragments arranged by size: 2629 2504 884 496 325 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6265 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4288 5049 6077 1540 Size: 275 186 2287 761 1028 2301 Fragments arranged by size: 2301 2287 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 3687 4899 4984 6055 2256 Size: 1431 1212 85 1071 3039 Fragments arranged by size: 3039 1431 1212 1071 85 BsmI GAATG_Cn' Cuts at: 4349 4442 4349 Size: 93 6745 BsmBI CGTCTCn'nnnn_ Cuts at: 2146 2146 Size: 6838 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2256 2341 2364 3687 3936 4157 Size: 399 412 54 85 23 1323 249 221 Cuts at: 4157 4899 4984 6055 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1323 1071 742 412 399 249 221 85 85 54 23 BspEI T'CCGG_A Cuts at: 2060 6501 2060 Size: 4441 2397 BspHI T'CATG_A Cuts at: 4727 6457 4727 Size: 1730 5108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4193 844 Size: 3349 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2343 4725 1394 Size: 429 520 2382 3507 Fragments arranged by size: 3507 2382 520 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5333 5507 65 Size: 1183 4085 174 1396 Fragments arranged by size: 4085 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5482 5669 1700 Size: 110 3672 187 2869 Fragments arranged by size: 3672 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6838 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6838 BssSI C'ACGA_G Cuts at: 4898 5910 4898 Size: 1012 5826 BstAPI GCAn_nnn'nTGC Cuts at: 794 3720 794 Size: 2926 3912 BstBI TT'CG_AA Cuts at: 5642 5642 Size: 6838 BstEII G'GTnAC_C Cuts at: 1184 2507 1184 Size: 1323 5515 BstYI r'GATC_y Cuts at: 1972 2055 2450 2462 2527 3312 4168 4535 4920 Size: 83 395 12 65 785 856 367 385 Cuts at: 4920 4937 6389 6475 6487 6833 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 856 785 395 385 367 346 86 83 65 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2085 2636 2085 Size: 551 6287 BtgI C'CryG_G Cuts at: 513 1065 2004 2429 3976 513 Size: 552 939 425 1547 3375 Fragments arranged by size: 3375 1547 939 552 425 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6099 Fragments arranged by size: 6099 683 56 BtsI GCAGTG_nn' Cuts at: 3703 3969 4354 5148 5168 3703 Size: 266 385 794 20 5373 Fragments arranged by size: 5373 794 385 266 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6838 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6803 DraI TTT'AAA Cuts at: 2608 4142 2608 Size: 1534 5304 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5845 809 Size: 507 4529 1802 Fragments arranged by size: 4529 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3994 4285 5174 Size: 54 1425 50 275 18 2164 291 889 Cuts at: 5174 6803 8 Size: 1629 43 Fragments arranged by size: 2164 1629 1425 889 291 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4285 1537 Size: 275 2473 4090 Fragments arranged by size: 4090 2473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4768 1353 Size: 3415 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5425 5799 5945 387 Size: 1064 138 3836 374 146 1280 Fragments arranged by size: 3836 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2367 2576 4901 5498 6285 6553 Size: 393 566 169 209 2325 597 787 268 Cuts at: 6553 1239 Size: 1524 Fragments arranged by size: 2325 1524 787 597 566 393 268 209 169 EcoO109I rG'GnC_Cy Cuts at: 2439 2576 2670 2439 Size: 137 94 6607 Fragments arranged by size: 6607 137 94 EcoRI G'AATT_C Cuts at: 4147 4525 4147 Size: 378 6460 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3970 3970 Size: 6838 FspI TGC'GCA Cuts at: 5344 5344 Size: 6838 HaeII r_GCGC'y Cuts at: 1678 5985 1678 Size: 4307 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2987 3019 3084 3116 5479 Size: 32 135 32 1415 32 65 32 2363 Cuts at: 5479 5511 5553 5585 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2363 1415 135 65 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4182 4428 669 Size: 988 281 2244 246 3079 Fragments arranged by size: 3079 2244 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6838 HpaI GTT'AAC Cuts at: 4428 4428 Size: 6838 KpnI G_GTAC'C Cuts at: 4163 4163 Size: 6838 MfeI C'AATT_G Cuts at: 4437 4437 Size: 6838 MluI A'CGCG_T Cuts at: 6714 6714 Size: 6838 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5952 6136 1305 Size: 4647 184 2007 Fragments arranged by size: 4647 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3996 10 Size: 54 1425 343 2164 2852 Fragments arranged by size: 2852 2164 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2799 4796 5155 5314 518 Size: 648 1633 1997 359 159 2042 Fragments arranged by size: 2042 1997 1633 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2052 2235 2431 4041 4254 4931 6079 6324 Size: 446 183 196 1610 213 677 1148 245 Cuts at: 6324 1606 Size: 2120 Fragments arranged by size: 2120 1610 1148 677 446 245 213 196 183 NcoI C'CATG_G Cuts at: 513 1065 2004 3976 513 Size: 552 939 1972 3375 Fragments arranged by size: 3375 1972 939 552 NdeI CA'TA_TG Cuts at: 387 2730 387 Size: 2343 4495 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6838 NotI GC'GGCC_GC Cuts at: 4285 4285 Size: 6838 NsiI A_TGCA'T Cuts at: 2708 2708 Size: 6838 NspI r_CATG'y Cuts at: 1132 1229 2804 3498 3809 4196 5741 1132 Size: 97 1575 694 311 387 1545 2229 Fragments arranged by size: 2229 1575 1545 694 387 311 97 PasI CC'CwG_GG Cuts at: 1348 2436 1348 Size: 1088 5750 PciI A'CATG_T Cuts at: 3494 5737 3494 Size: 2243 4595 PflMI CCAn_nnn'nTGG Cuts at: 1108 2442 1108 Size: 1334 5504 PmeI GTTT'AAAC Cuts at: 4142 4142 Size: 6838 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4907 4939 6420 6452 4907 Size: 32 1481 32 5293 Fragments arranged by size: 5293 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2576 2670 2576 Size: 94 6744 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6838 PsiI TTA'TAA Cuts at: 4408 4408 Size: 6838 PspOMI G'GGCC_C Cuts at: 2337 2337 Size: 6838 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4091 4123 4091 Size: 32 6806 PstI C_TGCA'G Cuts at: 830 4190 830 Size: 3360 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6838 PvuII CAG'CTG Cuts at: 4041 4041 Size: 6838 SacII CC_GC'GG Cuts at: 2432 2432 Size: 6838 SalI G'TCGA_C Cuts at: 1936 4180 1936 Size: 2244 4594 SanDI GG'GwC_CC Cuts at: 2576 2670 2576 Size: 94 6744 SbfI CC_TGCA'GG Cuts at: 4190 4190 Size: 6838 ScaI AGT'ACT Cuts at: 5086 6798 5086 Size: 1712 5126 SfcI C'TryA_G Cuts at: 826 1045 2829 3081 3662 4087 4186 5321 6002 Size: 219 1784 252 581 425 99 1135 681 Cuts at: 6002 6193 826 Size: 191 1471 Fragments arranged by size: 1784 1471 1135 681 581 425 252 219 191 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6838 SmaI CCC'GGG Cuts at: 1872 2336 4165 1872 Size: 464 1829 4545 Fragments arranged by size: 4545 1829 464 SmlI C'TyrA_G Cuts at: 820 1017 1943 3791 4942 5843 6105 820 Size: 197 926 1848 1151 901 262 1553 Fragments arranged by size: 1848 1553 1151 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4629 493 Size: 4136 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6838 SphI G_CATG'C Cuts at: 1132 4196 1132 Size: 3064 3774 SspI AAT'ATT Cuts at: 5 52 3375 4762 5 Size: 47 3323 1387 2081 Fragments arranged by size: 3323 2081 1387 47 StuI AGG'CCT Cuts at: 1517 2482 3087 1517 Size: 965 605 5268 Fragments arranged by size: 5268 965 605 StyI C'CwwG_G Cuts at: 513 1065 2004 3688 3852 3976 4062 4277 513 Size: 552 939 1684 164 124 86 215 3074 Fragments arranged by size: 3074 1684 939 552 215 164 124 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4865 4882 5035 5220 5665 325 Size: 1255 3285 17 153 185 445 1498 Fragments arranged by size: 3285 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3258 5084 6796 Size: 274 80 33 51 1443 1281 1826 1712 Cuts at: 6796 96 Size: 138 Fragments arranged by size: 1826 1712 1443 1281 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 6798 6830 2270 Size: 32 4496 32 2278 Fragments arranged by size: 4496 2278 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6838 XbaI T'CTAG_A Cuts at: 4126 4174 4126 Size: 48 6790 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3220 3220 Size: 6838 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6838 XmaI C'CCGG_G Cuts at: 1870 2334 4163 1870 Size: 464 1829 4545 Fragments arranged by size: 4545 1829 464 XmnI GAAnn'nnTTC Cuts at: 3170 4967 3170 Size: 1797 5041 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4646 276 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BbvCI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI