Restriction map for
pFN21AE5726
(Circular) (Six-base) MAPSORT of: pFN21AE5726.seq Check: 6923 from: 1 to: 6838
pFN21AE5726 6838 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2832 3661 3740 4181 1937
Size: 895 829 79 441 4594
Fragments arranged by size:
4594 895 829 441 79
Acc65I G'GTAC_C
Cuts at: 4159 4159
Size: 6838
AclI AA'CG_TT
Cuts at: 1563 4965 5338 1563
Size: 3402 373 3063
Fragments arranged by size:
3402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4901 6285 6553 1632
Size: 3269 1384 268 1917
Fragments arranged by size:
3269 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6641
AflIII A'CryG_T
Cuts at: 1203 3494 5737 6714 1203
Size: 2291 2243 977 1327
Fragments arranged by size:
2291 2243 1327 977
AgeI A'CCGG_T
Cuts at: 5669 5669
Size: 6838
AhdI GACnn_n'nnGTC
Cuts at: 5567 5567
Size: 6838
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6838
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2719 3366 1184
Size: 1535 647 4656
Fragments arranged by size:
4656 1535 647
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 6806
AlwNI CAG_nnn'CTG
Cuts at: 2442 4581 6153 2442
Size: 2139 1572 3127
Fragments arranged by size:
3127 2139 1572
ApaI G_GGCC'C
Cuts at: 2341 2341
Size: 6838
ApaLI G'TGCA_C
Cuts at: 4895 6051 4895
Size: 1156 5682
ApoI r'AATT_y
Cuts at: 1493 2925 4147 4373 4525 6634 1493
Size: 1432 1222 226 152 2109 1697
Fragments arranged by size:
2109 1697 1432 1222 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3831 3969 5392 160
Size: 872 2799 138 1423 1606
Fragments arranged by size:
2799 1606 1423 872 138
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 2334 4163 1870
Size: 73 391 1829 4545
Fragments arranged by size:
4545 1829 391 73
AvrII C'CTAG_G
Cuts at: 3688 3688
Size: 6838
BamHI G'GATC_C
Cuts at: 2450 2527 3312 4168 4535 2450
Size: 77 785 856 367 4753
Fragments arranged by size:
4753 856 785 367 77
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4159 5614 618
Size: 325 217 627 2372 1455 1842
Fragments arranged by size:
2372 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2202 2341 2364 4157 1391
Size: 811 139 23 1793 4072
Fragments arranged by size:
4072 1793 811 139 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 3000 3231 928
Size: 971 240 861 231 4535
Fragments arranged by size:
4535 971 861 240 231
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5029 5063 1903
Size: 34 3092 34 3678
Fragments arranged by size:
3678 3092 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4730 5940 4730
Size: 1210 5628
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6838
BfrBI ATG'CAT
Cuts at: 2706 2706
Size: 6838
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5449 136
Size: 107 122 71 631 605 3777 1525
Fragments arranged by size:
3777 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6833 6833
Size: 6838
BlpI GC'TnA_GC
Cuts at: 4255 4255
Size: 6838
Bme1580I G_kGCm'C
Cuts at: 1790 2341 3936 4899 6055 1790
Size: 551 1595 963 1156 2573
Fragments arranged by size:
2573 1595 1156 963 551
BmgBI CAC'GTC
Cuts at: 1318 2156 2223 3382 1318
Size: 838 67 1159 4774
Fragments arranged by size:
4774 1159 838 67
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4569 5527 442
Size: 416 53 83 399 24 3152 958 1753
Fragments arranged by size:
3152 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6838
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3291 3291
Size: 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2367 2576 5498 1239
Size: 959 169 209 2922 2579
Fragments arranged by size:
2922 2579 959 209 169
Bpu10I CC'TnA_GC
Cuts at: 2539 2539
Size: 6838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3776 4963 5828 6126 3776
Size: 1187 865 298 4488
Fragments arranged by size:
4488 1187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5501 882
Size: 617 4002 2219
Fragments arranged by size:
4002 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2716 4629 493
Size: 711 1512 1913 2702
Fragments arranged by size:
2702 1913 1512 711
BsaBI GATnn'nnATC
Cuts at: 3311 6832 3311
Size: 3521 3317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2144 4645 5027 5662
Size: 53 83 186 936 611 2501 382 635
Cuts at: 5662 275
Size: 1451
Fragments arranged by size:
2501 1451 936 635 611 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2060 5271 5669 5943 6090 6501 2060
Size: 3211 398 274 147 411 2397
Fragments arranged by size:
3211 2397 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 2549 2579 1178
Size: 30 165 30 867 30 249 30 5437
Fragments arranged by size:
5437 867 249 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2088 2102 2132 2180 2225 2278 3267 1186
Size: 902 14 30 48 45 53 989 4757
Fragments arranged by size:
4757 989 902 53 48 45 30 14
BseYI C'CCAG_C
Cuts at: 2328 3212 3537 6041 6537 2328
Size: 884 325 2504 496 2629
Fragments arranged by size:
2629 2504 884 496 325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6265
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4288 5049 6077 1540
Size: 275 186 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 3687 4899 4984 6055 2256
Size: 1431 1212 85 1071 3039
Fragments arranged by size:
3039 1431 1212 1071 85
BsmI GAATG_Cn'
Cuts at: 4349 4442 4349
Size: 93 6745
BsmBI CGTCTCn'nnnn_
Cuts at: 2146 2146
Size: 6838
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2256 2341 2364 3687 3936 4157
Size: 399 412 54 85 23 1323 249 221
Cuts at: 4157 4899 4984 6055 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1323 1071 742 412 399 249 221
85 85 54 23
BspEI T'CCGG_A
Cuts at: 2060 6501 2060
Size: 4441 2397
BspHI T'CATG_A
Cuts at: 4727 6457 4727
Size: 1730 5108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4193 844
Size: 3349 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2343 4725 1394
Size: 429 520 2382 3507
Fragments arranged by size:
3507 2382 520 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5333 5507 65
Size: 1183 4085 174 1396
Fragments arranged by size:
4085 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5482 5669 1700
Size: 110 3672 187 2869
Fragments arranged by size:
3672 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6838
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6838
BssSI C'ACGA_G
Cuts at: 4898 5910 4898
Size: 1012 5826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3720 794
Size: 2926 3912
BstBI TT'CG_AA
Cuts at: 5642 5642
Size: 6838
BstEII G'GTnAC_C
Cuts at: 1184 2507 1184
Size: 1323 5515
BstYI r'GATC_y
Cuts at: 1972 2055 2450 2462 2527 3312 4168 4535 4920
Size: 83 395 12 65 785 856 367 385
Cuts at: 4920 4937 6389 6475 6487 6833 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 856 785 395 385 367 346
86 83 65 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 2085 2636 2085
Size: 551 6287
BtgI C'CryG_G
Cuts at: 513 1065 2004 2429 3976 513
Size: 552 939 425 1547 3375
Fragments arranged by size:
3375 1547 939 552 425
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6099
Fragments arranged by size:
6099 683 56
BtsI GCAGTG_nn'
Cuts at: 3703 3969 4354 5148 5168 3703
Size: 266 385 794 20 5373
Fragments arranged by size:
5373 794 385 266 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6803
DraI TTT'AAA
Cuts at: 2608 4142 2608
Size: 1534 5304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5845 809
Size: 507 4529 1802
Fragments arranged by size:
4529 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3994 4285 5174
Size: 54 1425 50 275 18 2164 291 889
Cuts at: 5174 6803 8
Size: 1629 43
Fragments arranged by size:
2164 1629 1425 889 291 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4285 1537
Size: 275 2473 4090
Fragments arranged by size:
4090 2473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4768 1353
Size: 3415 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5425 5799 5945 387
Size: 1064 138 3836 374 146 1280
Fragments arranged by size:
3836 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2367 2576 4901 5498 6285 6553
Size: 393 566 169 209 2325 597 787 268
Cuts at: 6553 1239
Size: 1524
Fragments arranged by size:
2325 1524 787 597 566 393 268 209
169
EcoO109I rG'GnC_Cy
Cuts at: 2439 2576 2670 2439
Size: 137 94 6607
Fragments arranged by size:
6607 137 94
EcoRI G'AATT_C
Cuts at: 4147 4525 4147
Size: 378 6460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3970 3970
Size: 6838
FspI TGC'GCA
Cuts at: 5344 5344
Size: 6838
HaeII r_GCGC'y
Cuts at: 1678 5985 1678
Size: 4307 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2987 3019 3084 3116 5479
Size: 32 135 32 1415 32 65 32 2363
Cuts at: 5479 5511 5553 5585 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2363 1415 135 65 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4182 4428 669
Size: 988 281 2244 246 3079
Fragments arranged by size:
3079 2244 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6838
HpaI GTT'AAC
Cuts at: 4428 4428
Size: 6838
KpnI G_GTAC'C
Cuts at: 4163 4163
Size: 6838
MfeI C'AATT_G
Cuts at: 4437 4437
Size: 6838
MluI A'CGCG_T
Cuts at: 6714 6714
Size: 6838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5952 6136 1305
Size: 4647 184 2007
Fragments arranged by size:
4647 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3996 10
Size: 54 1425 343 2164 2852
Fragments arranged by size:
2852 2164 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2799 4796 5155 5314 518
Size: 648 1633 1997 359 159 2042
Fragments arranged by size:
2042 1997 1633 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2235 2431 4041 4254 4931 6079 6324
Size: 446 183 196 1610 213 677 1148 245
Cuts at: 6324 1606
Size: 2120
Fragments arranged by size:
2120 1610 1148 677 446 245 213 196
183
NcoI C'CATG_G
Cuts at: 513 1065 2004 3976 513
Size: 552 939 1972 3375
Fragments arranged by size:
3375 1972 939 552
NdeI CA'TA_TG
Cuts at: 387 2730 387
Size: 2343 4495
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6838
NotI GC'GGCC_GC
Cuts at: 4285 4285
Size: 6838
NsiI A_TGCA'T
Cuts at: 2708 2708
Size: 6838
NspI r_CATG'y
Cuts at: 1132 1229 2804 3498 3809 4196 5741 1132
Size: 97 1575 694 311 387 1545 2229
Fragments arranged by size:
2229 1575 1545 694 387 311 97
PasI CC'CwG_GG
Cuts at: 1348 2436 1348
Size: 1088 5750
PciI A'CATG_T
Cuts at: 3494 5737 3494
Size: 2243 4595
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2442 1108
Size: 1334 5504
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 6420 6452 4907
Size: 32 1481 32 5293
Fragments arranged by size:
5293 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2576 2670 2576
Size: 94 6744
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6838
PsiI TTA'TAA
Cuts at: 4408 4408
Size: 6838
PspOMI G'GGCC_C
Cuts at: 2337 2337
Size: 6838
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4091 4123 4091
Size: 32 6806
PstI C_TGCA'G
Cuts at: 830 4190 830
Size: 3360 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
PvuII CAG'CTG
Cuts at: 4041 4041
Size: 6838
SacII CC_GC'GG
Cuts at: 2432 2432
Size: 6838
SalI G'TCGA_C
Cuts at: 1936 4180 1936
Size: 2244 4594
SanDI GG'GwC_CC
Cuts at: 2576 2670 2576
Size: 94 6744
SbfI CC_TGCA'GG
Cuts at: 4190 4190
Size: 6838
ScaI AGT'ACT
Cuts at: 5086 6798 5086
Size: 1712 5126
SfcI C'TryA_G
Cuts at: 826 1045 2829 3081 3662 4087 4186 5321 6002
Size: 219 1784 252 581 425 99 1135 681
Cuts at: 6002 6193 826
Size: 191 1471
Fragments arranged by size:
1784 1471 1135 681 581 425 252 219
191 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6838
SmaI CCC'GGG
Cuts at: 1872 2336 4165 1872
Size: 464 1829 4545
Fragments arranged by size:
4545 1829 464
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3791 4942 5843 6105 820
Size: 197 926 1848 1151 901 262 1553
Fragments arranged by size:
1848 1553 1151 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4629 493
Size: 4136 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6838
SphI G_CATG'C
Cuts at: 1132 4196 1132
Size: 3064 3774
SspI AAT'ATT
Cuts at: 5 52 3375 4762 5
Size: 47 3323 1387 2081
Fragments arranged by size:
3323 2081 1387 47
StuI AGG'CCT
Cuts at: 1517 2482 3087 1517
Size: 965 605 5268
Fragments arranged by size:
5268 965 605
StyI C'CwwG_G
Cuts at: 513 1065 2004 3688 3852 3976 4062 4277 513
Size: 552 939 1684 164 124 86 215 3074
Fragments arranged by size:
3074 1684 939 552 215 164 124 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4865 4882 5035 5220 5665 325
Size: 1255 3285 17 153 185 445 1498
Fragments arranged by size:
3285 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3258 5084 6796
Size: 274 80 33 51 1443 1281 1826 1712
Cuts at: 6796 96
Size: 138
Fragments arranged by size:
1826 1712 1443 1281 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 6798 6830 2270
Size: 32 4496 32 2278
Fragments arranged by size:
4496 2278 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6838
XbaI T'CTAG_A
Cuts at: 4126 4174 4126
Size: 48 6790
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3220 3220
Size: 6838
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6838
XmaI C'CCGG_G
Cuts at: 1870 2334 4163 1870
Size: 464 1829 4545
Fragments arranged by size:
4545 1829 464
XmnI GAAnn'nnTTC
Cuts at: 3170 4967 3170
Size: 1797 5041
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BsiWI BstXI
BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI
RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI