Restriction map for
pFN21AE5722
(Circular) (Six-base) MAPSORT of: pFN21AE5722.seq Check: 8600 from: 1 to: 7315
pFN21AE5722 7315 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5125 278
Size: 53 83 186 936 3589 2468
Fragments arranged by size:
3589 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4321 4658 1937
Size: 2384 337 4594
Fragments arranged by size:
4594 2384 337
Acc65I G'GTAC_C
Cuts at: 4636 4636
Size: 7315
AclI AA'CG_TT
Cuts at: 1563 5442 5815 1563
Size: 3879 373 3063
Fragments arranged by size:
3879 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 2534 2602 3531 5378 6762 7030 1632
Size: 593 309 68 929 1847 1384 268 1917
Fragments arranged by size:
1917 1847 1384 929 593 309 268 68
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7118
AflIII A'CryG_T
Cuts at: 1203 6214 7191 1203
Size: 5011 977 1327
Fragments arranged by size:
5011 1327 977
AgeI A'CCGG_T
Cuts at: 6146 6146
Size: 7315
AhdI GACnn_n'nnGTC
Cuts at: 2438 6044 2438
Size: 3606 3709
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3471 1184
Size: 2287 5028
AlwNI CAG_nnn'CTG
Cuts at: 3458 5058 6630 3458
Size: 1600 1572 4143
Fragments arranged by size:
4143 1600 1572
ApaI G_GGCC'C
Cuts at: 4068 4068
Size: 7315
ApaLI G'TGCA_C
Cuts at: 2025 5372 6528 2025
Size: 3347 1156 2812
Fragments arranged by size:
3347 2812 1156
ApoI r'AATT_y
Cuts at: 1493 2288 2317 3926 4103 4115 4490 4624 4850
Size: 795 29 1609 177 12 375 134 226
Cuts at: 4850 5002 7111 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1609 795 375 226 177 152
134 29 12
AseI AT'TA_AT
Cuts at: 160 1032 5869 160
Size: 872 4837 1606
Fragments arranged by size:
4837 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
AvaI C'yCGr_G
Cuts at: 1870 1943 4640 1870
Size: 73 2697 4545
Fragments arranged by size:
4545 2697 73
AvrII C'CTAG_G
Cuts at: 3154 3154
Size: 7315
BamHI G'GATC_C
Cuts at: 3590 4645 5012 3590
Size: 1055 367 5893
Fragments arranged by size:
5893 1055 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4636 6091 618
Size: 325 217 627 2849 1455 1842
Fragments arranged by size:
2849 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3387 3581 4068 4634 1391
Size: 1996 194 487 566 4072
Fragments arranged by size:
4072 1996 566 487 194
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2560 3842 3999 928
Size: 971 661 1282 157 4244
Fragments arranged by size:
4244 1282 971 661 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5506 5540 1903
Size: 34 3569 34 3678
Fragments arranged by size:
3678 3569 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5207 6417 5207
Size: 1210 6105
BclI T'GATC_A
Cuts at: 1548 2080 1548
Size: 532 6783
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5926 136
Size: 107 122 71 631 605 4254 1525
Fragments arranged by size:
4254 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7310 7310
Size: 7315
BlpI GC'TnA_GC
Cuts at: 4732 4732
Size: 7315
Bme1580I G_kGCm'C
Cuts at: 1790 2029 3829 4068 5376 6532 1790
Size: 239 1800 239 1308 1156 2573
Fragments arranged by size:
2573 1800 1308 1156 239 239
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7315
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5046 6004 442
Size: 416 53 83 399 24 3629 958 1753
Fragments arranged by size:
3629 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7315
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3722
Size: 7315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2676 2958 3371 3782 3882 5975 1239
Size: 1088 349 282 413 411 100 2093 2579
Fragments arranged by size:
2579 2093 1088 413 411 349 282 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5440 6305 6603 5440
Size: 865 298 6152
Fragments arranged by size:
6152 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2564 3390 4407 5978 882
Size: 617 1065 826 1017 1571 2219
Fragments arranged by size:
2219 1571 1065 1017 826 617
BsaAI yAC'GTr
Cuts at: 493 1204 5106 493
Size: 711 3902 2702
Fragments arranged by size:
3902 2702 711
BsaBI GATnn'nnATC
Cuts at: 7309 7309
Size: 7315
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5122 5504 6139 275
Size: 53 83 186 936 3589 382 635 1451
Fragments arranged by size:
3589 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2031 3034 3587 5748 6146 6420 6567 6978 2031
Size: 1003 553 2161 398 274 147 411 2368
Fragments arranged by size:
2368 2161 1003 553 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3016 3046 3269 3299 1178
Size: 30 165 30 1613 30 223 30 5194
Fragments arranged by size:
5194 1613 223 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2190 2709 3116 3408 3791 1186
Size: 1004 519 407 292 383 4710
Fragments arranged by size:
4710 1004 519 407 383 292
BseYI C'CCAG_C
Cuts at: 2342 3794 4365 6518 7014 2342
Size: 1452 571 2153 496 2643
Fragments arranged by size:
2643 2153 1452 571 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2685 3260 3444 4301 1163
Size: 573 949 575 184 857 4177
Fragments arranged by size:
4177 949 857 575 573 184
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4049 4765 5526 6554 1540
Size: 275 186 2048 716 761 1028 2301
Fragments arranged by size:
2301 2048 1028 761 716 275 186
BsiHKAI G_wGCw'C
Cuts at: 2029 3387 3581 4230 5376 5461 6532 2029
Size: 1358 194 649 1146 85 1071 2812
Fragments arranged by size:
2812 1358 1146 1071 649 194 85
BsmI GAATG_Cn'
Cuts at: 4826 4919 4826
Size: 93 7222
BsmBI CGTCTCn'nnnn_
Cuts at: 3247 3247
Size: 7315
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 3387 3581 3829 4068 4230 4634
Size: 399 239 1358 194 248 239 162 404
Cuts at: 4634 5376 5461 6532 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1358 1071 742 404 399 248 239
239 194 162 85
BspEI T'CCGG_A
Cuts at: 2031 3034 3587 6978 2031
Size: 1003 553 3391 2368
Fragments arranged by size:
3391 2368 1003 553
BspHI T'CATG_A
Cuts at: 5204 6934 5204
Size: 1730 5585
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4670 844
Size: 3826 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5202 1394
Size: 429 3379 3507
Fragments arranged by size:
3507 3379 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4377 5810 5984 65
Size: 1183 3129 1433 174 1396
Fragments arranged by size:
3129 1433 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4048 4244 5959 6146 1700
Size: 110 2238 196 1715 187 2869
Fragments arranged by size:
2869 2238 1715 196 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7315
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7315
BssSI C'ACGA_G
Cuts at: 2126 3443 5375 6387 2126
Size: 1317 1932 1012 3054
Fragments arranged by size:
3054 1932 1317 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3458 794
Size: 2664 4651
BstBI TT'CG_AA
Cuts at: 3875 6119 3875
Size: 2244 5071
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7315
BstXI CCAn_nnnn'nTGG
Cuts at: 3295 4405 3295
Size: 1110 6205
BstYI r'GATC_y
Cuts at: 1972 3402 3590 4645 5012 5397 5414 6866 6952
Size: 1430 188 1055 367 385 17 1452 86
Cuts at: 6952 6964 7310 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1430 1055 385 367 346 188
86 17 12
BstZ17I GTA'TAC
Cuts at: 4322 4322
Size: 7315
BtgI C'CryG_G
Cuts at: 513 1065 2170 3430 513
Size: 552 1105 1260 4398
Fragments arranged by size:
4398 1260 1105 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6576
Fragments arranged by size:
6576 683 56
BtsI GCAGTG_nn'
Cuts at: 4831 5625 5645 4831
Size: 794 20 6501
Fragments arranged by size:
6501 794 20
ClaI AT'CG_AT
Cuts at: 1554 2053 1554
Size: 499 6816
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3643 3678 529
Size: 35 3079 35 4166
Fragments arranged by size:
4166 3079 35 35
DraI TTT'AAA
Cuts at: 2826 3691 4619 2826
Size: 865 928 5522
Fragments arranged by size:
5522 928 865
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6322 809
Size: 507 5006 1802
Fragments arranged by size:
5006 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4046 4246 4762
Size: 54 1425 50 275 18 2216 200 516
Cuts at: 4762 5651 7280 8
Size: 889 1629 43
Fragments arranged by size:
2216 1629 1425 889 516 275 200 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4046 4762 1537
Size: 275 2234 716 4090
Fragments arranged by size:
4090 2234 716 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2775 4563 5245 1353
Size: 1422 1788 682 3423
Fragments arranged by size:
3423 1788 1422 682
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5902 6276 6422 387
Size: 1064 138 4313 374 146 1280
Fragments arranged by size:
4313 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3385 3579 3385
Size: 194 7121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2327 2534 2602 2676 2958 3371
Size: 393 593 102 207 68 74 282 413
Cuts at: 3371 3531 3782 3882 5378 5975 6762 7030 1239
Size: 160 251 100 1496 597 787 268 1524
Fragments arranged by size:
1524 1496 787 597 593 413 393 282
268 251 207 160 102 100 74 68
EcoNI CCTnn'n_nnAGG
Cuts at: 3981 3981
Size: 7315
EcoO109I rG'GnC_Cy
Cuts at: 2600 4064 2600
Size: 1464 5851
EcoRI G'AATT_C
Cuts at: 2317 4103 4624 5002 2317
Size: 1786 521 378 4630
Fragments arranged by size:
4630 1786 521 378
EcoRV GAT'ATC
Cuts at: 2057 4098 4219 2057
Size: 2041 121 5153
Fragments arranged by size:
5153 2041 121
FspI TGC'GCA
Cuts at: 5821 5821
Size: 7315
HaeII r_GCGC'y
Cuts at: 1678 6462 1678
Size: 4784 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2341 2373 2424 2456 3169
Size: 32 135 32 769 32 51 32 713
Cuts at: 3169 3201 3387 3419 4082 4114 5956 5988 6030
Size: 32 186 32 663 32 1842 32 42
Cuts at: 6030 6062 1373
Size: 32 2626
Fragments arranged by size:
2626 1842 769 713 663 186 135 51
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2233 3013 4659 4905 669
Size: 988 281 295 780 1646 246 3079
Fragments arranged by size:
3079 1646 988 780 295 281 246
HindIII A'AGCT_T
Cuts at: 748 3118 4556 748
Size: 2370 1438 3507
Fragments arranged by size:
3507 2370 1438
HpaI GTT'AAC
Cuts at: 4905 4905
Size: 7315
KpnI G_GTAC'C
Cuts at: 4640 4640
Size: 7315
MfeI C'AATT_G
Cuts at: 4914 4914
Size: 7315
MluI A'CGCG_T
Cuts at: 7191 7191
Size: 7315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3256 6429 6613 1305
Size: 1951 3173 184 2007
Fragments arranged by size:
3173 2007 1951 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5493
Fragments arranged by size:
5493 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 2852 5273 5632 5791 518
Size: 648 1009 677 2421 359 159 2042
Fragments arranged by size:
2421 2042 1009 677 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3455 4731 5408 6556 6801 1606
Size: 1849 1276 677 1148 245 2120
Fragments arranged by size:
2120 1849 1276 1148 677 245
NaeI GCC'GGC
Cuts at: 4246 4246
Size: 7315
NcoI C'CATG_G
Cuts at: 513 1065 2170 513
Size: 552 1105 5658
Fragments arranged by size:
5658 1105 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7315
NgoMIV G'CCGG_C
Cuts at: 4244 4244
Size: 7315
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7315
NotI GC'GGCC_GC
Cuts at: 4762 4762
Size: 7315
NspI r_CATG'y
Cuts at: 1132 1229 2019 2135 3007 4673 6218 1132
Size: 97 790 116 872 1666 1545 2229
Fragments arranged by size:
2229 1666 1545 872 790 116 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7315
PciI A'CATG_T
Cuts at: 6214 6214
Size: 7315
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7315
PfoI T'CCnGG_A
Cuts at: 2653 2974 2653
Size: 321 6994
PmeI GTTT'AAAC
Cuts at: 4619 4619
Size: 7315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5384 5416 6897 6929 5384
Size: 32 1481 32 5770
Fragments arranged by size:
5770 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7315
PsiI TTA'TAA
Cuts at: 4885 4885
Size: 7315
PspOMI G'GGCC_C
Cuts at: 4064 4064
Size: 7315
PstI C_TGCA'G
Cuts at: 830 2419 4667 830
Size: 1589 2248 3478
Fragments arranged by size:
3478 2248 1589
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7315
PvuII CAG'CTG
Cuts at: 3455 3455
Size: 7315
SacI G_AGCT'C
Cuts at: 3387 3581 3387
Size: 194 7121
SalI G'TCGA_C
Cuts at: 1936 4657 1936
Size: 2721 4594
SapI GCTCTTCn'nnn_
Cuts at: 4563 4563
Size: 7315
SbfI CC_TGCA'GG
Cuts at: 4667 4667
Size: 7315
ScaI AGT'ACT
Cuts at: 5563 7275 5563
Size: 1712 5603
SfcI C'TryA_G
Cuts at: 826 1045 2415 2755 4663 5798 6479 6670 826
Size: 219 1370 340 1908 1135 681 191 1471
Fragments arranged by size:
1908 1471 1370 1135 681 340 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7315
SmaI CCC'GGG
Cuts at: 1872 4642 1872
Size: 2770 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5419 6320 6582 820
Size: 197 926 3476 901 262 1553
Fragments arranged by size:
3476 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5106 493
Size: 4613 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7315
SphI G_CATG'C
Cuts at: 1132 4673 1132
Size: 3541 3774
SspI AAT'ATT
Cuts at: 5 52 2796 4502 5239 5
Size: 47 2744 1706 737 2081
Fragments arranged by size:
2744 2081 1706 737 47
StuI AGG'CCT
Cuts at: 1517 3031 3159 1517
Size: 1514 128 5673
Fragments arranged by size:
5673 1514 128
StyI C'CwwG_G
Cuts at: 513 1065 2170 3154 4519 4754 513
Size: 552 1105 984 1365 235 3074
Fragments arranged by size:
3074 1365 1105 984 552 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2980 5342 5359 5512 5697 6142 325
Size: 1255 1400 2362 17 153 185 445 1498
Fragments arranged by size:
2362 1498 1400 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4251 5561 7273
Size: 274 80 33 51 1443 2274 1310 1712
Cuts at: 7273 96
Size: 138
Fragments arranged by size:
2274 1712 1443 1310 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3016 3048 3269 3301 3468 3500 3912 3944 4263
Size: 32 221 32 167 32 412 32 319
Cuts at: 4263 4295 7275 7307 3016
Size: 32 2980 32 3024
Fragments arranged by size:
3024 2980 412 319 221 167 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7315
XbaI T'CTAG_A
Cuts at: 4651 4651
Size: 7315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2651 2921 4204 2651
Size: 270 1283 5762
Fragments arranged by size:
5762 1283 270
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7315
XmaI C'CCGG_G
Cuts at: 1870 4640 1870
Size: 2770 4545
XmnI GAAnn'nnTTC
Cuts at: 5444 5444
Size: 7315
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5123 276
Size: 53 83 186 936 3589 2468
Fragments arranged by size:
3589 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI Bsu36I DraIII FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI