Restriction map for
pFN21AE5721
(Circular) (Six-base) MAPSORT of: pFN21AE5721.seq Check: 1135 from: 1 to: 6775
pFN21AE5721 6775 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4585 278
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3003 3054 3071 3501 3826 3906 4118 1937
Size: 1066 51 17 430 325 80 212 4594
Fragments arranged by size:
4594 1066 430 325 212 80 51 17
Acc65I G'GTAC_C
Cuts at: 4096 4096
Size: 6775
AclI AA'CG_TT
Cuts at: 1563 3408 4902 5275 1563
Size: 1845 1494 373 3063
Fragments arranged by size:
3063 1845 1494 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2181 2881 4838 6222 6490 1632
Size: 549 700 1957 1384 268 1917
Fragments arranged by size:
1957 1917 1384 700 549 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6578
AflIII A'CryG_T
Cuts at: 1203 5674 6651 1203
Size: 4471 977 1327
Fragments arranged by size:
4471 1327 977
AgeI A'CCGG_T
Cuts at: 5606 5606
Size: 6775
AhdI GACnn_n'nnGTC
Cuts at: 5504 5504
Size: 6775
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6775
AlwNI CAG_nnn'CTG
Cuts at: 2251 3967 4518 6090 2251
Size: 1716 551 1572 2936
Fragments arranged by size:
2936 1716 1572 551
ApaI G_GGCC'C
Cuts at: 4062 4062
Size: 6775
ApaLI G'TGCA_C
Cuts at: 4832 5988 4832
Size: 1156 5619
ApoI r'AATT_y
Cuts at: 1493 3026 3230 3995 4022 4084 4310 4462 6571
Size: 1533 204 765 27 62 226 152 2109
Cuts at: 6571 1493
Size: 1697
Fragments arranged by size:
2109 1697 1533 765 226 204 152 62
27
AseI AT'TA_AT
Cuts at: 160 1032 3015 5329 160
Size: 872 1983 2314 1606
Fragments arranged by size:
2314 1983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
AvaI C'yCGr_G
Cuts at: 1870 1943 2485 4100 1870
Size: 73 542 1615 4545
Fragments arranged by size:
4545 1615 542 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2985 3018 2985
Size: 33 6742
BamHI G'GATC_C
Cuts at: 4105 4472 4105
Size: 367 6408
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2258 4096 5551 618
Size: 325 217 627 471 1838 1455 1842
Fragments arranged by size:
1842 1838 1455 627 471 325 217
BanII G_rGCy'C
Cuts at: 1391 4062 4094 1391
Size: 2671 32 4072
Fragments arranged by size:
4072 2671 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5804
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3616 3650 4966 5000 1903
Size: 34 1679 34 1316 34 3678
Fragments arranged by size:
3678 1679 1316 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4667 5877 4667
Size: 1210 5565
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6775
BfrBI ATG'CAT
Cuts at: 2948 3268 2948
Size: 320 6455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5386 136
Size: 107 122 71 631 605 3714 1525
Fragments arranged by size:
3714 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3868 6770 3868
Size: 2902 3873
BlpI GC'TnA_GC
Cuts at: 4192 4192
Size: 6775
Bme1580I G_kGCm'C
Cuts at: 1790 4062 4836 5992 1790
Size: 2272 774 1156 2573
Fragments arranged by size:
2573 2272 1156 774
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6775
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4506 5464 442
Size: 416 53 83 399 24 3089 958 1753
Fragments arranged by size:
3089 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3531 4065 5435 1239
Size: 2292 534 1370 2579
Fragments arranged by size:
2579 2292 1370 534
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4900 5765 6063 4900
Size: 865 298 5612
Fragments arranged by size:
5612 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5438 882
Size: 617 3939 2219
Fragments arranged by size:
3939 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2620 3052 4566 493
Size: 711 1416 432 1514 2702
Fragments arranged by size:
2702 1514 1416 711 432
BsaBI GATnn'nnATC
Cuts at: 6769 6769
Size: 6775
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4582 4964 5599 275
Size: 53 83 186 936 3049 382 635 1451
Fragments arranged by size:
3049 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5208 5606 5880 6027 6438 5208
Size: 398 274 147 411 5545
Fragments arranged by size:
5545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3091 3121 1178
Size: 30 165 30 1688 30 4832
Fragments arranged by size:
4832 1688 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 2468 1186
Size: 1255 27 5493
Fragments arranged by size:
5493 1255 27
BseYI C'CCAG_C
Cuts at: 5978 6474 5978
Size: 496 6279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6202
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4225 4986 6014 1540
Size: 275 186 2224 761 1028 2301
Fragments arranged by size:
2301 2224 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4836 4921 5992 4836
Size: 85 1071 5619
Fragments arranged by size:
5619 1071 85
BsmI GAATG_Cn'
Cuts at: 2950 3220 3648 4286 4379 2950
Size: 270 428 638 93 5346
Fragments arranged by size:
5346 638 428 270 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4062 4094 4836 4921 5992 1391
Size: 399 2272 32 742 85 1071 2174
Fragments arranged by size:
2272 2174 1071 742 399 85 32
BspEI T'CCGG_A
Cuts at: 6438 6438
Size: 6775
BspHI T'CATG_A
Cuts at: 4664 6394 4664
Size: 1730 5045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 4130 844
Size: 1415 1871 3489
Fragments arranged by size:
3489 1871 1415
BsrBI CCG'CTC
Cuts at: 1394 1823 4049 4662 1394
Size: 429 2226 613 3507
Fragments arranged by size:
3507 2226 613 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2262 3840 5270 5444 65
Size: 1183 1014 1578 1430 174 1396
Fragments arranged by size:
1578 1430 1396 1183 1014 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5419 5606 1700
Size: 110 3609 187 2869
Fragments arranged by size:
3609 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2535 3552 96
Size: 2439 1017 3319
Fragments arranged by size:
3319 2439 1017
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6775
BssSI C'ACGA_G
Cuts at: 4835 5847 4835
Size: 1012 5763
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2251 3265 3398 794
Size: 1457 1014 133 4171
Fragments arranged by size:
4171 1457 1014 133
BstBI TT'CG_AA
Cuts at: 3274 5579 3274
Size: 2305 4470
BstEII G'GTnAC_C
Cuts at: 1184 2669 1184
Size: 1485 5290
BstYI r'GATC_y
Cuts at: 1972 3832 3868 4105 4472 4857 4874 6326 6412
Size: 1860 36 237 367 385 17 1452 86
Cuts at: 6412 6424 6770 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1860 1452 385 367 346 237 86
36 17 12
BstZ17I GTA'TAC
Cuts at: 3055 3055
Size: 6775
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3636 487
Size: 683 56 2410 3626
Fragments arranged by size:
3626 2410 683 56
BtsI GCAGTG_nn'
Cuts at: 3862 4291 5085 5105 3862
Size: 429 794 20 5532
Fragments arranged by size:
5532 794 429 20
ClaI AT'CG_AT
Cuts at: 1554 2643 1554
Size: 1089 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6740
DraI TTT'AAA
Cuts at: 2743 4079 2743
Size: 1336 5439
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5782 809
Size: 507 4466 1802
Fragments arranged by size:
4466 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3031 3690 4222
Size: 54 1425 50 275 18 1201 659 532
Cuts at: 4222 5111 6740 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1201 889 659 532 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4222 1537
Size: 275 2410 4090
Fragments arranged by size:
4090 2410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2336 2445 3786 3870 4705 1353
Size: 983 109 1341 84 835 3423
Fragments arranged by size:
3423 1341 983 835 109 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5362 5736 5882 387
Size: 1064 138 3773 374 146 1280
Fragments arranged by size:
3773 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2881 3531 4065 4838 5435 6222
Size: 393 549 700 650 534 773 597 787
Cuts at: 6222 6490 1239
Size: 268 1524
Fragments arranged by size:
1524 787 773 700 650 597 549 534
393 268
EcoO109I rG'GnC_Cy
Cuts at: 4040 4040
Size: 6775
EcoRI G'AATT_C
Cuts at: 4084 4462 4084
Size: 378 6397
EcoRV GAT'ATC
Cuts at: 3538 3538
Size: 6775
FspI TGC'GCA
Cuts at: 5281 5281
Size: 6775
HaeII r_GCGC'y
Cuts at: 1678 5922 1678
Size: 4244 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2606 2638 2741
Size: 32 135 32 563 32 439 32 103
Cuts at: 2741 2773 2869 2901 3935 3967 5416 5448 5490
Size: 32 96 32 1034 32 1449 32 42
Cuts at: 5490 5522 1373
Size: 32 2626
Fragments arranged by size:
2626 1449 1034 563 439 135 103 96
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4119 4365 669
Size: 988 281 2181 246 3079
Fragments arranged by size:
3079 2181 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6775
HpaI GTT'AAC
Cuts at: 4365 4365
Size: 6775
KpnI G_GTAC'C
Cuts at: 4100 4100
Size: 6775
MfeI C'AATT_G
Cuts at: 4374 4374
Size: 6775
MluI A'CGCG_T
Cuts at: 6651 6651
Size: 6775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2754 2931 3231 5889 6073 1305
Size: 1449 177 300 2658 184 2007
Fragments arranged by size:
2658 2007 1449 300 184 177
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4953
Fragments arranged by size:
4953 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2943 4733 5092 5251 518
Size: 648 1777 1790 359 159 2042
Fragments arranged by size:
2042 1790 1777 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4191 4868 6016 6261 1606
Size: 2585 677 1148 245 2120
Fragments arranged by size:
2585 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6223
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6775
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6775
NotI GC'GGCC_GC
Cuts at: 4222 4222
Size: 6775
NsiI A_TGCA'T
Cuts at: 2950 3270 2950
Size: 320 6455
NspI r_CATG'y
Cuts at: 1132 1229 2121 2568 2952 4133 5678 1132
Size: 97 892 447 384 1181 1545 2229
Fragments arranged by size:
2229 1545 1181 892 447 384 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6775
PciI A'CATG_T
Cuts at: 5674 5674
Size: 6775
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6775
PfoI T'CCnGG_A
Cuts at: 3508 3508
Size: 6775
PmeI GTTT'AAAC
Cuts at: 4079 4079
Size: 6775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2694 2726 4844 4876 6357 6389 2694
Size: 32 2118 32 1481 32 3080
Fragments arranged by size:
3080 2118 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4040 4040
Size: 6775
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6775
PsiI TTA'TAA
Cuts at: 2208 4345 2208
Size: 2137 4638
PspOMI G'GGCC_C
Cuts at: 4058 4058
Size: 6775
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3178 3210 3178
Size: 32 6743
PstI C_TGCA'G
Cuts at: 830 4127 830
Size: 3297 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6775
SalI G'TCGA_C
Cuts at: 1936 4117 1936
Size: 2181 4594
SapI GCTCTTCn'nnn_
Cuts at: 2336 2336
Size: 6775
SbfI CC_TGCA'GG
Cuts at: 4127 4127
Size: 6775
ScaI AGT'ACT
Cuts at: 5023 6735 5023
Size: 1712 5063
SexAI A'CCwGG_T
Cuts at: 3920 3920
Size: 6775
SfcI C'TryA_G
Cuts at: 826 1045 3502 4071 4123 5258 5939 6130 826
Size: 219 2457 569 52 1135 681 191 1471
Fragments arranged by size:
2457 1471 1135 681 569 219 191 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6775
SmaI CCC'GGG
Cuts at: 1872 4102 1872
Size: 2230 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4879 5780 6042 820
Size: 197 926 2936 901 262 1553
Fragments arranged by size:
2936 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2620 3052 4566 493
Size: 2127 432 1514 2702
Fragments arranged by size:
2702 2127 1514 432
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6775
SphI G_CATG'C
Cuts at: 1132 2121 4133 1132
Size: 989 2012 3774
Fragments arranged by size:
3774 2012 989
SspI AAT'ATT
Cuts at: 5 52 2029 2600 3697 4699 5
Size: 47 1977 571 1097 1002 2081
Fragments arranged by size:
2081 1977 1097 1002 571 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6775
StyI C'CwwG_G
Cuts at: 513 1065 3984 4214 513
Size: 552 2919 230 3074
Fragments arranged by size:
3074 2919 552 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4802 4819 4972 5157 5602 325
Size: 1255 3222 17 153 185 445 1498
Fragments arranged by size:
3222 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2535 3552 5021
Size: 274 80 33 51 1443 558 1017 1469
Cuts at: 5021 6733 96
Size: 1712 138
Fragments arranged by size:
1712 1469 1443 1017 558 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2935 2967 6735 6767 2935
Size: 32 3768 32 2943
Fragments arranged by size:
3768 2943 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2630 1316
Size: 1314 5461
XbaI T'CTAG_A
Cuts at: 4111 4111
Size: 6775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3784 3784
Size: 6775
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6775
XmaI C'CCGG_G
Cuts at: 1870 4100 1870
Size: 2230 4545
XmnI GAAnn'nnTTC
Cuts at: 4904 4904
Size: 6775
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4583 276
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspXI PvuII RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI