Restriction map for
pFN21AE5720
(Circular) (Six-base) MAPSORT of: pFN21AE5720.seq Check: 6824 from: 1 to: 6553
pFN21AE5720 6553 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4363 278
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2466 3896 1937
Size: 529 1430 4594
Fragments arranged by size:
4594 1430 529
Acc65I G'GTAC_C
Cuts at: 3874 3874
Size: 6553
AclI AA'CG_TT
Cuts at: 1563 3429 4680 5053 1563
Size: 1866 1251 373 3063
Fragments arranged by size:
3063 1866 1251 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4616 6000 6268 1632
Size: 2984 1384 268 1917
Fragments arranged by size:
2984 1917 1384 268
AfeI AGC'GCT
Cuts at: 2283 2283
Size: 6553
AflII C'TTAA_G
Cuts at: 820 1017 3271 3524 820
Size: 197 2254 253 3849
Fragments arranged by size:
3849 2254 253 197
AflIII A'CryG_T
Cuts at: 1203 5452 6429 1203
Size: 4249 977 1327
Fragments arranged by size:
4249 1327 977
AgeI A'CCGG_T
Cuts at: 5384 5384
Size: 6553
AhdI GACnn_n'nnGTC
Cuts at: 5282 5282
Size: 6553
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6553
AlwNI CAG_nnn'CTG
Cuts at: 4296 5868 4296
Size: 1572 4981
ApaLI G'TGCA_C
Cuts at: 2050 4610 5766 2050
Size: 2560 1156 2837
Fragments arranged by size:
2837 2560 1156
ApoI r'AATT_y
Cuts at: 1493 2080 2719 3862 4088 4240 6349 1493
Size: 587 639 1143 226 152 2109 1697
Fragments arranged by size:
2109 1697 1143 639 587 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5107 160
Size: 872 4075 1606
Fragments arranged by size:
4075 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
AvaI C'yCGr_G
Cuts at: 1870 1943 3878 1870
Size: 73 1935 4545
Fragments arranged by size:
4545 1935 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2345 2378 3259 3292 2345
Size: 33 881 33 5606
Fragments arranged by size:
5606 881 33 33
BamHI G'GATC_C
Cuts at: 3127 3883 4250 3127
Size: 756 367 5430
Fragments arranged by size:
5430 756 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3874 5329 618
Size: 325 217 627 2087 1455 1842
Fragments arranged by size:
2087 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2241 3872 1391
Size: 850 1631 4072
Fragments arranged by size:
4072 1631 850
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2016 928
Size: 971 117 5465
Fragments arranged by size:
5465 971 117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4744 4778 1903
Size: 34 2807 34 3678
Fragments arranged by size:
3678 2807 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2373 4445 5655 2373
Size: 2072 1210 3271
Fragments arranged by size:
3271 2072 1210
BclI T'GATC_A
Cuts at: 1548 3223 1548
Size: 1675 4878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5164 136
Size: 107 122 71 631 605 3492 1525
Fragments arranged by size:
3492 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2947 6548 2947
Size: 3601 2952
BlpI GC'TnA_GC
Cuts at: 3970 3970
Size: 6553
Bme1580I G_kGCm'C
Cuts at: 1790 2054 4614 5770 1790
Size: 264 2560 1156 2573
Fragments arranged by size:
2573 2560 1156 264
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6553
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2307 4284 5242
Size: 416 53 83 399 24 890 1977 958
Cuts at: 5242 442
Size: 1753
Fragments arranged by size:
1977 1753 958 890 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2130 2292 3059 3811 5213 1239
Size: 891 162 767 752 1402 2579
Fragments arranged by size:
2579 1402 891 767 752 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4678 5543 5841 4678
Size: 865 298 5390
Fragments arranged by size:
5390 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5216 882
Size: 617 3717 2219
Fragments arranged by size:
3717 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4344 493
Size: 711 3140 2702
Fragments arranged by size:
3140 2702 711
BsaBI GATnn'nnATC
Cuts at: 6547 6547
Size: 6553
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4360 4742 5377 275
Size: 53 83 186 936 2827 382 635 1451
Fragments arranged by size:
2827 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4986 5384 5658 5805 6216 4986
Size: 398 274 147 411 5323
Fragments arranged by size:
5323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3032 3062 1178
Size: 30 165 30 1629 30 4669
Fragments arranged by size:
4669 1629 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3230 3366 1186
Size: 2044 136 4373
Fragments arranged by size:
4373 2044 136
BseYI C'CCAG_C
Cuts at: 3595 5756 6252 3595
Size: 2161 496 3896
Fragments arranged by size:
3896 2161 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5980
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4003 4764 5792 1540
Size: 275 186 2002 761 1028 2301
Fragments arranged by size:
2301 2002 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2054 4614 4699 5770 2054
Size: 2560 85 1071 2837
Fragments arranged by size:
2837 2560 1071 85
BsmI GAATG_Cn'
Cuts at: 4064 4157 4064
Size: 93 6460
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2241 3872 4614 4699 5770 1391
Size: 399 264 187 1631 742 85 1071 2174
Fragments arranged by size:
2174 1631 1071 742 399 264 187 85
BspEI T'CCGG_A
Cuts at: 6216 6216
Size: 6553
BspHI T'CATG_A
Cuts at: 4442 6172 4442
Size: 1730 4823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3908 844
Size: 3064 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4440 1394
Size: 429 2617 3507
Fragments arranged by size:
3507 2617 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5048 5222 65
Size: 1183 3800 174 1396
Fragments arranged by size:
3800 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5197 5384 1700
Size: 110 3387 187 2869
Fragments arranged by size:
3387 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6553
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6553
BssSI C'ACGA_G
Cuts at: 4613 5625 4613
Size: 1012 5541
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3320 3781 794
Size: 2526 461 3566
Fragments arranged by size:
3566 2526 461
BstBI TT'CG_AA
Cuts at: 5357 5357
Size: 6553
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6553
BstXI CCAn_nnnn'nTGG
Cuts at: 2092 2092
Size: 6553
BstYI r'GATC_y
Cuts at: 1972 2947 3127 3883 4250 4635 4652 6104 6190
Size: 975 180 756 367 385 17 1452 86
Cuts at: 6190 6202 6548 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 975 756 385 367 346 180
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3135 513
Size: 552 939 1131 3931
Fragments arranged by size:
3931 1131 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5814
Fragments arranged by size:
5814 683 56
BtsI GCAGTG_nn'
Cuts at: 2257 3089 4069 4863 4883 2257
Size: 832 980 794 20 3927
Fragments arranged by size:
3927 980 832 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6553
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6518
DraI TTT'AAA
Cuts at: 2025 3857 2025
Size: 1832 4721
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2212 5560 809
Size: 507 896 3348 1802
Fragments arranged by size:
3348 1802 896 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2764 4000 4889
Size: 54 1425 50 275 18 934 1236 889
Cuts at: 4889 6518 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1236 934 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4000 1537
Size: 275 2188 4090
Fragments arranged by size:
4090 2188 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2223 2854 2989 3388 4483 1353
Size: 870 631 135 399 1095 3423
Fragments arranged by size:
3423 1095 870 631 399 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5140 5514 5660 387
Size: 1064 138 3551 374 146 1280
Fragments arranged by size:
3551 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2292 3059 3811 4616 5213 6000
Size: 393 498 162 767 752 805 597 787
Cuts at: 6000 6268 1239
Size: 268 1524
Fragments arranged by size:
1524 805 787 767 752 597 498 393
268 162
EcoNI CCTnn'n_nnAGG
Cuts at: 3790 3790
Size: 6553
EcoO109I rG'GnC_Cy
Cuts at: 2104 2104
Size: 6553
EcoRI G'AATT_C
Cuts at: 2080 3862 4240 2080
Size: 1782 378 4393
Fragments arranged by size:
4393 1782 378
EcoRV GAT'ATC
Cuts at: 3798 3798
Size: 6553
FspI TGC'GCA
Cuts at: 5059 5059
Size: 6553
HaeII r_GCGC'y
Cuts at: 1678 2285 5700 1678
Size: 607 3415 2531
Fragments arranged by size:
3415 2531 607
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2732 2764 3163 3195 3233
Size: 32 135 32 1160 32 399 32 38
Cuts at: 3233 3265 3478 3510 3787 3819 5194 5226 5268
Size: 32 213 32 277 32 1375 32 42
Cuts at: 5268 5300 1373
Size: 32 2626
Fragments arranged by size:
2626 1375 1160 399 277 213 135 42
38 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3361 3897 4143 669
Size: 988 281 1423 536 246 3079
Fragments arranged by size:
3079 1423 988 536 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6553
HpaI GTT'AAC
Cuts at: 4143 4143
Size: 6553
KpnI G_GTAC'C
Cuts at: 3878 3878
Size: 6553
MfeI C'AATT_G
Cuts at: 4152 4152
Size: 6553
MluI A'CGCG_T
Cuts at: 6429 6429
Size: 6553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2110 3412 5667 5851 1305
Size: 805 1302 2255 184 2007
Fragments arranged by size:
2255 2007 1302 805 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2766 10
Size: 54 1425 343 934 3797
Fragments arranged by size:
3797 1425 934 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3237 4511 4870 5029 518
Size: 648 2071 1274 359 159 2042
Fragments arranged by size:
2071 2042 1274 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2359 3311 3969 4646 5794 6039 1606
Size: 753 952 658 677 1148 245 2120
Fragments arranged by size:
2120 1148 952 753 677 658 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3135 513
Size: 552 939 1131 3931
Fragments arranged by size:
3931 1131 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6553
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6553
NotI GC'GGCC_GC
Cuts at: 4000 4000
Size: 6553
NspI r_CATG'y
Cuts at: 1132 1229 3784 3911 5456 1132
Size: 97 2555 127 1545 2229
Fragments arranged by size:
2555 2229 1545 127 97
PasI CC'CwG_GG
Cuts at: 1348 2606 1348
Size: 1258 5295
PciI A'CATG_T
Cuts at: 5452 5452
Size: 6553
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3835 1108
Size: 2727 3826
PmeI GTTT'AAAC
Cuts at: 3857 3857
Size: 6553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4622 4654 6135 6167 4622
Size: 32 1481 32 5008
Fragments arranged by size:
5008 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2104
Size: 6553
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6553
PsiI TTA'TAA
Cuts at: 4123 4123
Size: 6553
PstI C_TGCA'G
Cuts at: 830 3905 830
Size: 3075 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6553
PvuII CAG'CTG
Cuts at: 2359 3311 2359
Size: 952 5601
SalI G'TCGA_C
Cuts at: 1936 3895 1936
Size: 1959 4594
SbfI CC_TGCA'GG
Cuts at: 3905 3905
Size: 6553
ScaI AGT'ACT
Cuts at: 4801 6513 4801
Size: 1712 4841
SfcI C'TryA_G
Cuts at: 826 1045 2323 2497 2965 3280 3470 3619 3901
Size: 219 1278 174 468 315 190 149 282
Cuts at: 3901 5036 5717 5908 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1278 1135 681 468 315 282 219
191 190 174 149
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6553
SmaI CCC'GGG
Cuts at: 1872 3880 1872
Size: 2008 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3271 3524 4657 5558 5820 820
Size: 197 926 1328 253 1133 901 262 1553
Fragments arranged by size:
1553 1328 1133 926 901 262 253 197
SnaBI TAC'GTA
Cuts at: 493 4344 493
Size: 3851 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6553
SphI G_CATG'C
Cuts at: 1132 3911 1132
Size: 2779 3774
SspI AAT'ATT
Cuts at: 5 52 3652 4477 5
Size: 47 3600 825 2081
Fragments arranged by size:
3600 2081 825 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6553
StyI C'CwwG_G
Cuts at: 513 1065 2004 3135 3148 3442 3992 513
Size: 552 939 1131 13 294 550 3074
Fragments arranged by size:
3074 1131 939 552 550 294 13
SwaI ATTT'AAAT
Cuts at: 2025 2025
Size: 6553
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2510 2953 4580 4597 4750 4935 5380
Size: 1255 930 443 1627 17 153 185 445
Cuts at: 5380 325
Size: 1498
Fragments arranged by size:
1627 1498 1255 930 445 443 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4799 6511 96
Size: 274 80 33 51 1443 2822 1712 138
Fragments arranged by size:
2822 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6513 6545 6513
Size: 32 6521
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6553
XbaI T'CTAG_A
Cuts at: 3889 3889
Size: 6553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2515 2515
Size: 6553
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6553
XmaI C'CCGG_G
Cuts at: 1870 3878 1870
Size: 2008 4545
XmnI GAAnn'nnTTC
Cuts at: 4682 4682
Size: 6553
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4361 276
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI