Restriction map for
pFN21AE5718
(Circular) (Six-base) MAPSORT of: pFN21AE5718.seq Check: 4556 from: 1 to: 7618
pFN21AE5718 7618 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5428 278
Size: 53 83 186 936 3892 2468
Fragments arranged by size:
3892 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4316 4961 1937
Size: 2379 645 4594
Fragments arranged by size:
4594 2379 645
Acc65I G'GTAC_C
Cuts at: 4939 4939
Size: 7618
AclI AA'CG_TT
Cuts at: 1563 5745 6118 1563
Size: 4182 373 3063
Fragments arranged by size:
4182 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2360 2753 2908 3153 4751 5681 7065
Size: 464 264 393 155 245 1598 930 1384
Cuts at: 7065 7333 1632
Size: 268 1917
Fragments arranged by size:
1917 1598 1384 930 464 393 268 264
245 155
AflII C'TTAA_G
Cuts at: 820 1017 3556 820
Size: 197 2539 4882
Fragments arranged by size:
4882 2539 197
AflIII A'CryG_T
Cuts at: 1203 6517 7494 1203
Size: 5314 977 1327
Fragments arranged by size:
5314 1327 977
AgeI A'CCGG_T
Cuts at: 6449 6449
Size: 7618
AhdI GACnn_n'nnGTC
Cuts at: 2016 6347 2016
Size: 4331 3287
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7618
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7618
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3246 3278 4082 4114 3246
Size: 32 804 32 6750
Fragments arranged by size:
6750 804 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3778 3800 5361 6933 3778
Size: 22 1561 1572 4463
Fragments arranged by size:
4463 1572 1561 22
ApaLI G'TGCA_C
Cuts at: 5675 6831 5675
Size: 1156 6462
ApoI r'AATT_y
Cuts at: 1493 2114 2493 2874 2883 3298 3581 4927 5153
Size: 621 379 381 9 415 283 1346 226
Cuts at: 5153 5305 7414 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1346 621 415 381 379 283
226 152 9
AseI AT'TA_AT
Cuts at: 160 1032 6172 160
Size: 872 5140 1606
Fragments arranged by size:
5140 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7618
AvaI C'yCGr_G
Cuts at: 1870 1943 4943 1870
Size: 73 3000 4545
Fragments arranged by size:
4545 3000 73
BamHI G'GATC_C
Cuts at: 4079 4526 4948 5315 4079
Size: 447 422 367 6382
Fragments arranged by size:
6382 447 422 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3292 3695 4939 6394 618
Size: 325 217 627 1505 403 1244 1455 1842
Fragments arranged by size:
1842 1505 1455 1244 627 403 325 217
BanII G_rGCy'C
Cuts at: 1391 2521 2739 4326 4937 1391
Size: 1130 218 1587 611 4072
Fragments arranged by size:
4072 1587 1130 611 218
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5809 5843 1903
Size: 34 50 34 3788 34 3678
Fragments arranged by size:
3788 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2979 5510 6720 2979
Size: 2531 1210 3877
Fragments arranged by size:
3877 2531 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7618
BfrBI ATG'CAT
Cuts at: 3807 3807
Size: 7618
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4046 6229 136
Size: 107 122 71 631 605 2374 2183 1525
Fragments arranged by size:
2374 2183 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3010 3840 4249 7613 3010
Size: 830 409 3364 3015
Fragments arranged by size:
3364 3015 830 409
BlpI GC'TnA_GC
Cuts at: 4776 5035 4776
Size: 259 7359
Bme1580I G_kGCm'C
Cuts at: 1790 3700 5679 6835 1790
Size: 1910 1979 1156 2573
Fragments arranged by size:
2573 1979 1910 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7618
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3835 5349 6307
Size: 416 53 83 399 24 2418 1514 958
Cuts at: 6307 442
Size: 1753
Fragments arranged by size:
2418 1753 1514 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7618
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3873 3873
Size: 7618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2900 3641 6278 1239
Size: 1661 741 2637 2579
Fragments arranged by size:
2637 2579 1661 741
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3178 4274 5743 6608 6906 3178
Size: 1096 1469 865 298 3890
Fragments arranged by size:
3890 1469 1096 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6281 882
Size: 617 4782 2219
Fragments arranged by size:
4782 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5409 493
Size: 711 4205 2702
Fragments arranged by size:
4205 2702 711
BsaBI GATnn'nnATC
Cuts at: 7612 7612
Size: 7618
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5425 5807 6442 275
Size: 53 83 186 936 3892 382 635 1451
Fragments arranged by size:
3892 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4076 6051 6449 6723 6870 7281 4076
Size: 1975 398 274 147 411 4413
Fragments arranged by size:
4413 1975 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2283 2313 2619 2649 3617
Size: 30 165 30 880 30 306 30 968
Cuts at: 3617 3647 4082 4112 1178
Size: 30 435 30 4684
Fragments arranged by size:
4684 968 880 435 306 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2163 3638 3876 4106 4109 4382 4883 1186
Size: 977 1475 238 230 3 273 501 3921
Fragments arranged by size:
3921 1475 977 501 273 238 230 3
BseYI C'CCAG_C
Cuts at: 2462 6821 7317 2462
Size: 4359 496 2763
Fragments arranged by size:
4359 2763 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 3408 1163
Size: 573 312 1360 5373
Fragments arranged by size:
5373 1360 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5068 5829 6857 1540
Size: 275 186 3067 761 1028 2301
Fragments arranged by size:
3067 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4326 5679 5764 6835 4326
Size: 1353 85 1071 5109
Fragments arranged by size:
5109 1353 1071 85
BsmI GAATG_Cn'
Cuts at: 2097 3809 4063 4300 5129 5222 2097
Size: 1712 254 237 829 93 4493
Fragments arranged by size:
4493 1712 829 254 237 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3678 3678
Size: 7618
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2521 2739 3700 4326 4937 5679 5764
Size: 399 731 218 961 626 611 742 85
Cuts at: 5764 6835 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 961 742 731 626 611 399
218 85
BspEI T'CCGG_A
Cuts at: 7281 7281
Size: 7618
BspHI T'CATG_A
Cuts at: 2089 5507 7237 2089
Size: 3418 1730 2470
Fragments arranged by size:
3418 2470 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 4973 844
Size: 1235 2894 3489
Fragments arranged by size:
3489 2894 1235
BsrBI CCG'CTC
Cuts at: 1394 1823 2616 5505 1394
Size: 429 793 2889 3507
Fragments arranged by size:
3507 2889 793 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2129 6113 6287 65
Size: 1183 881 3984 174 1396
Fragments arranged by size:
3984 1396 1183 881 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6262 6449 1700
Size: 110 4452 187 2869
Fragments arranged by size:
4452 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7618
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7618
BssSI C'ACGA_G
Cuts at: 2354 3788 5678 6690 2354
Size: 1434 1890 1012 3282
Fragments arranged by size:
3282 1890 1434 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7618
BstBI TT'CG_AA
Cuts at: 2887 6422 2887
Size: 3535 4083
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7618
BstXI CCAn_nnnn'nTGG
Cuts at: 3040 3040
Size: 7618
BstYI r'GATC_y
Cuts at: 1972 3010 3840 4079 4249 4526 4948 5315 5700
Size: 1038 830 239 170 277 422 367 385
Cuts at: 5700 5717 7169 7255 7267 7613 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1038 830 422 385 367 346
277 239 170 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2138 2157 2416 2593 4035 513
Size: 552 1073 19 259 177 1442 4096
Fragments arranged by size:
4096 1442 1073 552 259 177 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3253 487
Size: 683 56 2027 4852
Fragments arranged by size:
4852 2027 683 56
BtsI GCAGTG_nn'
Cuts at: 2212 5134 5928 5948 2212
Size: 2922 794 20 3882
Fragments arranged by size:
3882 2922 794 20
ClaI AT'CG_AT
Cuts at: 1554 2943 1554
Size: 1389 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2342 2377 529
Size: 35 1778 35 5770
Fragments arranged by size:
5770 1778 35 35
DraI TTT'AAA
Cuts at: 2343 4922 2343
Size: 2579 5039
DraIII CAC_nnn'GTG
Cuts at: 3289 3289
Size: 7618
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2803 6625 809
Size: 507 1487 3822 1802
Fragments arranged by size:
3822 1802 1487 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3816 4038 5065
Size: 54 1425 50 275 18 1986 222 1027
Cuts at: 5065 5954 7583 8
Size: 889 1629 43
Fragments arranged by size:
1986 1629 1425 1027 889 275 222 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5065 1537
Size: 275 3253 4090
Fragments arranged by size:
4090 3253 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 2808 3129 3596 4331 4727 5548 1353
Size: 897 558 321 467 735 396 821 3423
Fragments arranged by size:
3423 897 821 735 558 467 396 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6205 6579 6725 387
Size: 1064 138 4616 374 146 1280
Fragments arranged by size:
4616 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4324 4324
Size: 7618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2360 2753 2900 2908 3153 3641
Size: 393 464 264 393 147 8 245 488
Cuts at: 3641 4751 5681 6278 7065 7333 1239
Size: 1110 930 597 787 268 1524
Fragments arranged by size:
1524 1110 930 787 597 488 464 393
393 268 264 245 147 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2075 2075
Size: 7618
EcoO109I rG'GnC_Cy
Cuts at: 4879 4879
Size: 7618
EcoRI G'AATT_C
Cuts at: 2114 2493 2874 3581 4927 5305 2114
Size: 379 381 707 1346 378 4427
Fragments arranged by size:
4427 1346 707 381 379 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4796 4796
Size: 7618
FspI TGC'GCA
Cuts at: 2939 6124 2939
Size: 3185 4433
FspAI rTGC'GCAy
Cuts at: 2939 2939
Size: 7618
HaeII r_GCGC'y
Cuts at: 1678 6765 1678
Size: 5087 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2469 2501 2590
Size: 32 135 32 430 32 435 32 89
Cuts at: 2590 2622 2807 2839 2945 2977 3206 3238 3443
Size: 32 185 32 106 32 229 32 205
Cuts at: 3443 3475 3941 3973 4562 4594 6259 6291 6333
Size: 32 466 32 589 32 1665 32 42
Cuts at: 6333 6365 1373
Size: 32 2626
Fragments arranged by size:
2626 1665 589 466 435 430 229 205
185 135 106 89 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 2958 4962 5208 669
Size: 988 281 568 452 2004 246 3079
Fragments arranged by size:
3079 2004 988 568 452 281 246
HindIII A'AGCT_T
Cuts at: 748 2529 748
Size: 1781 5837
HpaI GTT'AAC
Cuts at: 5208 5208
Size: 7618
KpnI G_GTAC'C
Cuts at: 4943 4943
Size: 7618
MfeI C'AATT_G
Cuts at: 2232 3639 5217 2232
Size: 1407 1578 4633
Fragments arranged by size:
4633 1578 1407
MluI A'CGCG_T
Cuts at: 7494 7494
Size: 7618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6732 6916 1305
Size: 5427 184 2007
Fragments arranged by size:
5427 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3818 4040 10
Size: 54 1425 343 1986 222 3588
Fragments arranged by size:
3588 1986 1425 343 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2628 3038 5576 5935 6094 518
Size: 648 1462 410 2538 359 159 2042
Fragments arranged by size:
2538 2042 1462 648 410 359 159
MspA1I CmG'CkG
Cuts at: 1606 2195 5034 5711 6859 7104 1606
Size: 589 2839 677 1148 245 2120
Fragments arranged by size:
2839 2120 1148 677 589 245
NcoI C'CATG_G
Cuts at: 513 1065 2157 2416 4035 513
Size: 552 1092 259 1619 4096
Fragments arranged by size:
4096 1619 1092 552 259
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7618
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7618
NotI GC'GGCC_GC
Cuts at: 5065 5065
Size: 7618
NsiI A_TGCA'T
Cuts at: 3809 3809
Size: 7618
NspI r_CATG'y
Cuts at: 1132 1229 3370 4339 4976 6521 1132
Size: 97 2141 969 637 1545 2229
Fragments arranged by size:
2229 2141 1545 969 637 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7618
PciI A'CATG_T
Cuts at: 6517 6517
Size: 7618
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3825 4439 1108
Size: 2717 614 4287
Fragments arranged by size:
4287 2717 614
PfoI T'CCnGG_A
Cuts at: 2916 3915 4890 2916
Size: 999 975 5644
Fragments arranged by size:
5644 999 975
PmeI GTTT'AAAC
Cuts at: 4922 4922
Size: 7618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 5687 5719 7200 7232 4082
Size: 32 1573 32 1481 32 4468
Fragments arranged by size:
4468 1573 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4879 4879
Size: 7618
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7618
PsiI TTA'TAA
Cuts at: 5188 5188
Size: 7618
PstI C_TGCA'G
Cuts at: 830 4970 830
Size: 4140 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7618
PvuII CAG'CTG
Cuts at: 2195 2195
Size: 7618
SacI G_AGCT'C
Cuts at: 4326 4326
Size: 7618
SalI G'TCGA_C
Cuts at: 1936 4960 1936
Size: 3024 4594
SapI GCTCTTCn'nnn_
Cuts at: 3596 4331 3596
Size: 735 6883
SbfI CC_TGCA'GG
Cuts at: 4970 4970
Size: 7618
ScaI AGT'ACT
Cuts at: 5866 7578 5866
Size: 1712 5906
SfcI C'TryA_G
Cuts at: 826 1045 2745 4966 6101 6782 6973 826
Size: 219 1700 2221 1135 681 191 1471
Fragments arranged by size:
2221 1700 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4046 4046
Size: 7618
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7618
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7618
SmaI CCC'GGG
Cuts at: 1872 4945 1872
Size: 3073 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3157 3556 4253 5722 6623 6885
Size: 197 926 1214 399 697 1469 901 262
Cuts at: 6885 820
Size: 1553
Fragments arranged by size:
1553 1469 1214 926 901 697 399 262
197
SnaBI TAC'GTA
Cuts at: 493 5409 493
Size: 4916 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7618
SphI G_CATG'C
Cuts at: 1132 4976 1132
Size: 3844 3774
SspI AAT'ATT
Cuts at: 5 52 5542 5
Size: 47 5490 2081
Fragments arranged by size:
5490 2081 47
StuI AGG'CCT
Cuts at: 1517 3866 4631 1517
Size: 2349 765 4504
Fragments arranged by size:
4504 2349 765
StyI C'CwwG_G
Cuts at: 513 1065 2157 2416 2861 4035 4433 5057 513
Size: 552 1092 259 445 1174 398 624 3074
Fragments arranged by size:
3074 1174 1092 624 552 445 398 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 5645 5662 5815 6000 6445 325
Size: 1255 2088 1977 17 153 185 445 1498
Fragments arranged by size:
2088 1977 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4065 4174 4338
Size: 274 80 33 51 1443 2088 109 164
Cuts at: 4338 5864 7576 96
Size: 1526 1712 138
Fragments arranged by size:
2088 1712 1526 1443 274 164 138 109
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2283 2315 2970 3002 3617 3649 4435 4467 7578
Size: 32 655 32 615 32 786 32 3111
Cuts at: 7578 7610 2283
Size: 32 2291
Fragments arranged by size:
3111 2291 786 655 615 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7618
XbaI T'CTAG_A
Cuts at: 4864 4954 4864
Size: 90 7528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3114 3775 3967 3114
Size: 661 192 6765
Fragments arranged by size:
6765 661 192
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7618
XmaI C'CCGG_G
Cuts at: 1870 4943 1870
Size: 3073 4545
XmnI GAAnn'nnTTC
Cuts at: 2439 4159 4707 5747 2439
Size: 1720 548 1040 4310
Fragments arranged by size:
4310 1720 1040 548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5426 276
Size: 53 83 186 936 3892 2468
Fragments arranged by size:
3892 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I Bsu36I EcoRV FseI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfoI SrfI SwaI