Restriction map for
pFN21AE5717
(Circular) (Six-base) MAPSORT of: pFN21AE5717.seq Check: 6333 from: 1 to: 6676
pFN21AE5717 6676 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4486 278
Size: 53 83 186 936 2950 2468
Fragments arranged by size:
2950 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4019 1937
Size: 2082 4594
Acc65I G'GTAC_C
Cuts at: 3542 3997 3542
Size: 455 6221
AclI AA'CG_TT
Cuts at: 1563 4803 5176 1563
Size: 3240 373 3063
Fragments arranged by size:
3240 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2330 4739 6123 6391 1632
Size: 698 2409 1384 268 1917
Fragments arranged by size:
2409 1917 1384 698 268
AfeI AGC'GCT
Cuts at: 2600 2600
Size: 6676
AflII C'TTAA_G
Cuts at: 820 1017 3133 820
Size: 197 2116 4363
Fragments arranged by size:
4363 2116 197
AflIII A'CryG_T
Cuts at: 1203 2217 5575 6552 1203
Size: 1014 3358 977 1327
Fragments arranged by size:
3358 1327 1014 977
AgeI A'CCGG_T
Cuts at: 5507 5507
Size: 6676
AhdI GACnn_n'nnGTC
Cuts at: 2134 5405 2134
Size: 3271 3405
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6676
AlwNI CAG_nnn'CTG
Cuts at: 2199 2404 2611 3590 3650 3770 3830 4419 5991
Size: 205 207 979 60 120 60 589 1572
Cuts at: 5991 2199
Size: 2884
Fragments arranged by size:
2884 1572 979 589 207 205 120 60
60
ApaI G_GGCC'C
Cuts at: 2868 3039 3534 2868
Size: 171 495 6010
Fragments arranged by size:
6010 495 171
ApaLI G'TGCA_C
Cuts at: 2816 4733 5889 2816
Size: 1917 1156 3603
Fragments arranged by size:
3603 1917 1156
ApoI r'AATT_y
Cuts at: 1493 3241 3985 4211 4363 6472 1493
Size: 1748 744 226 152 2109 1697
Fragments arranged by size:
2109 1748 1697 744 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5230 160
Size: 872 4198 1606
Fragments arranged by size:
4198 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
AvaI C'yCGr_G
Cuts at: 1870 1943 2678 2915 2951 4001 1870
Size: 73 735 237 36 1050 4545
Fragments arranged by size:
4545 1050 735 237 73 36
BamHI G'GATC_C
Cuts at: 2541 4006 4373 2541
Size: 1465 367 4844
Fragments arranged by size:
4844 1465 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2692 3428 3471 3542 3997
Size: 325 217 627 905 736 43 71 455
Cuts at: 3997 5452 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 905 736 627 455 325 217
71 43
BanII G_rGCy'C
Cuts at: 1391 2523 2868 2924 3039 3060 3132 3534 3557
Size: 1132 345 56 115 21 72 402 23
Cuts at: 3557 3617 3677 3737 3797 3857 3995 1391
Size: 60 60 60 60 60 138 4072
Fragments arranged by size:
4072 1132 402 345 138 115 72 60
60 60 60 60 56 23 21
BbeI G_GCGC'C
Cuts at: 2696 2696
Size: 6676
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3945 928
Size: 971 2046 3659
Fragments arranged by size:
3659 2046 971
BbvCI CC'TCA_GC
Cuts at: 3260 3260
Size: 6676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2033 2067 4867 4901 1903
Size: 34 96 34 2800 34 3678
Fragments arranged by size:
3678 2800 96 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4568 5778 4568
Size: 1210 5466
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6676
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3356 5287 136
Size: 107 122 71 631 605 1684 1931 1525
Fragments arranged by size:
1931 1684 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6671 6671
Size: 6676
BlpI GC'TnA_GC
Cuts at: 3170 4093 3170
Size: 923 5753
Bme1580I G_kGCm'C
Cuts at: 1790 2570 2764 2820 2868 3039 3259 3433 3474
Size: 780 194 56 48 171 220 174 41
Cuts at: 3474 3534 4737 5893 1790
Size: 60 1203 1156 2573
Fragments arranged by size:
2573 1203 1156 780 220 194 174 171
60 56 48 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6676
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4407 5365 442
Size: 416 53 83 399 24 2990 958 1753
Fragments arranged by size:
2990 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2570 2735 2908 3098 3185 5336 1239
Size: 1331 165 173 190 87 2151 2579
Fragments arranged by size:
2579 2151 1331 190 173 165 87
Bpu10I CC'TnA_GC
Cuts at: 2456 3260 2456
Size: 804 5872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2396 2639 4801 5666 5964 2396
Size: 243 2162 865 298 3108
Fragments arranged by size:
3108 2162 865 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3557 3617 3677 3737 3797 3857 5339
Size: 617 2058 60 60 60 60 60 1482
Cuts at: 5339 882
Size: 2219
Fragments arranged by size:
2219 2058 1482 617 60 60 60 60
60
BsaAI yAC'GTr
Cuts at: 493 1204 4467 493
Size: 711 3263 2702
Fragments arranged by size:
3263 2702 711
BsaBI GATnn'nnATC
Cuts at: 6670 6670
Size: 6676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2693 4483 4865 5500
Size: 53 83 186 936 1160 1790 382 635
Cuts at: 5500 275
Size: 1451
Fragments arranged by size:
1790 1451 1160 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2242 5109 5507 5781 5928 6339 2242
Size: 2867 398 274 147 411 2579
Fragments arranged by size:
2867 2579 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 2372 2402 3339
Size: 30 165 30 606 30 333 30 937
Cuts at: 3339 3369 1178
Size: 30 4485
Fragments arranged by size:
4485 937 606 333 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2060 2147 2156 2732 2951 3097 3521
Size: 865 9 87 9 576 219 146 424
Cuts at: 3521 1186
Size: 4341
Fragments arranged by size:
4341 865 576 424 219 146 87 9
9
BseYI C'CCAG_C
Cuts at: 2162 2527 2662 2671 2894 3039 3398 3575 3580
Size: 365 135 9 223 145 359 177 5
Cuts at: 3580 3640 3695 3760 3875 5879 6375 2162
Size: 60 55 65 115 2004 496 2463
Fragments arranged by size:
2463 2004 496 365 359 223 177 145
135 115 65 60 55 9 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3344 1163
Size: 573 1608 4495
Fragments arranged by size:
4495 1608 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4126 4887 5915 1540
Size: 275 186 2125 761 1028 2301
Fragments arranged by size:
2301 2125 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2523 2584 2685 2820 2835 4737 4822 5893 2523
Size: 61 101 135 15 1902 85 1071 3306
Fragments arranged by size:
3306 1902 1071 135 101 85 61 15
BsmI GAATG_Cn'
Cuts at: 4187 4280 4187
Size: 93 6583
BsmBI CGTCTCn'nnnn_
Cuts at: 2714 2714
Size: 6676
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2523 2570 2584 2685 2764 2820 2835
Size: 399 733 47 14 101 79 56 15
Cuts at: 2835 2868 2924 3039 3060 3132 3259 3433 3474
Size: 33 56 115 21 72 127 174 41
Cuts at: 3474 3534 3557 3617 3677 3737 3797 3857 3995
Size: 60 23 60 60 60 60 60 138
Cuts at: 3995 4737 4822 5893 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 733 399 174 138 127
115 101 85 79 72 60 60 60
60 60 60 56 56 47 41 33
23 21 15 14
BspEI T'CCGG_A
Cuts at: 2242 6339 2242
Size: 4097 2579
BspHI T'CATG_A
Cuts at: 4565 6295 4565
Size: 1730 4946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3598 3658 3778 3838 4031 844
Size: 2754 60 120 60 193 3489
Fragments arranged by size:
3489 2754 193 120 60 60
BsrBI CCG'CTC
Cuts at: 1394 1823 2500 2914 4563 1394
Size: 429 677 414 1649 3507
Fragments arranged by size:
3507 1649 677 429 414
BsrDI GCAATG_nn'
Cuts at: 65 1248 2272 5171 5345 65
Size: 1183 1024 2899 174 1396
Fragments arranged by size:
2899 1396 1183 1024 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 3174 5320 5507 1700
Size: 110 286 1078 2146 187 2869
Fragments arranged by size:
2869 2146 1078 286 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6676
BssSI C'ACGA_G
Cuts at: 2209 2966 4736 5748 2209
Size: 757 1770 1012 3137
Fragments arranged by size:
3137 1770 1012 757
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3590 3650 3770 3830 794
Size: 2796 60 120 60 3640
Fragments arranged by size:
3640 2796 120 60 60
BstBI TT'CG_AA
Cuts at: 5480 5480
Size: 6676
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6676
BstXI CCAn_nnnn'nTGG
Cuts at: 2932 2932
Size: 6676
BstYI r'GATC_y
Cuts at: 1972 2245 2304 2490 2541 4006 4373 4758 4775
Size: 273 59 186 51 1465 367 385 17
Cuts at: 4775 6227 6313 6325 6671 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1465 1452 385 367 346 273 186
86 59 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 3275 3275
Size: 6676
BtgI C'CryG_G
Cuts at: 513 1065 2004 2147 3044 3109 513
Size: 552 939 143 897 65 4080
Fragments arranged by size:
4080 939 897 552 143 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5937
Fragments arranged by size:
5937 683 56
BtsI GCAGTG_nn'
Cuts at: 3327 4192 4986 5006 3327
Size: 865 794 20 4997
Fragments arranged by size:
4997 865 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6676
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6641
DraI TTT'AAA
Cuts at: 2476 3980 2476
Size: 1504 5172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5683 809
Size: 507 4367 1802
Fragments arranged by size:
4367 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3357 3417 4123
Size: 54 1425 50 275 18 1527 60 706
Cuts at: 4123 5012 6641 8
Size: 889 1629 43
Fragments arranged by size:
1629 1527 1425 889 706 275 60 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4123 1537
Size: 275 2311 4090
Fragments arranged by size:
4090 2311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3149 4606 1353
Size: 1796 1457 3423
Fragments arranged by size:
3423 1796 1457
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5263 5637 5783 387
Size: 1064 138 3674 374 146 1280
Fragments arranged by size:
3674 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2521 2521
Size: 6676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2570 2735 2908 3098 3185 4739
Size: 393 698 240 165 173 190 87 1554
Cuts at: 4739 5336 6123 6391 1239
Size: 597 787 268 1524
Fragments arranged by size:
1554 1524 787 698 597 393 268 240
190 173 165 87
EcoNI CCTnn'n_nnAGG
Cuts at: 2375 3263 2375
Size: 888 5788
EcoO109I rG'GnC_Cy
Cuts at: 2864 2865 2956 3035 3036 3071 3203 3285 3307
Size: 1 91 79 1 35 132 82 22
Cuts at: 3307 3453 3530 3572 3587 3632 3647 3692 3707
Size: 146 77 42 15 45 15 45 15
Cuts at: 3707 3752 3767 3812 3827 3872 3887 2864
Size: 45 15 45 15 45 15 5653
Fragments arranged by size:
5653 146 132 91 82 79 77 45
45 45 45 45 42 35 22 15
15 15 15 15 15 1 1
EcoRI G'AATT_C
Cuts at: 3985 4363 3985
Size: 378 6298
FspI TGC'GCA
Cuts at: 5182 5182
Size: 6676
HaeII r_GCGC'y
Cuts at: 1678 2602 2696 2806 5823 1678
Size: 924 94 110 3017 2531
Fragments arranged by size:
3017 2531 924 110 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2120 2152 2372
Size: 32 135 32 437 32 79 32 220
Cuts at: 2372 2404 3602 3634 3662 3694 3722 3754 3782
Size: 32 1198 32 28 32 28 32 28
Cuts at: 3782 3814 3842 3874 3935 3967 5317 5349 5391
Size: 32 28 32 61 32 1350 32 42
Cuts at: 5391 5423 1373
Size: 32 2626
Fragments arranged by size:
2626 1350 1198 437 220 135 79 61
42 32 32 32 32 32 32 32
32 32 32 32 32 32 28 28
28 28
HincII GTy'rAC
Cuts at: 669 1657 1938 4020 4266 669
Size: 988 281 2082 246 3079
Fragments arranged by size:
3079 2082 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6676
HpaI GTT'AAC
Cuts at: 4266 4266
Size: 6676
KasI G'GCGC_C
Cuts at: 2692 2692
Size: 6676
KpnI G_GTAC'C
Cuts at: 3546 4001 3546
Size: 455 6221
MfeI C'AATT_G
Cuts at: 4275 4275
Size: 6676
MluI A'CGCG_T
Cuts at: 6552 6552
Size: 6676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 2966 5790 5974 1305
Size: 813 848 2824 184 2007
Fragments arranged by size:
2824 2007 848 813 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3419 10
Size: 54 1425 343 1587 3267
Fragments arranged by size:
3267 1587 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4634 4993 5152 518
Size: 648 3468 359 159 2042
Fragments arranged by size:
3468 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2149 2698 2812 2847 4092 4769 5917 6162
Size: 543 549 114 35 1245 677 1148 245
Cuts at: 6162 1606
Size: 2120
Fragments arranged by size:
2120 1245 1148 677 549 543 245 114
35
NaeI GCC'GGC
Cuts at: 2098 3176 2098
Size: 1078 5598
NarI GG'CG_CC
Cuts at: 2693 2693
Size: 6676
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5185
Fragments arranged by size:
5185 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6676
NgoMIV G'CCGG_C
Cuts at: 2096 3174 2096
Size: 1078 5598
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6676
NotI GC'GGCC_GC
Cuts at: 4123 4123
Size: 6676
NspI r_CATG'y
Cuts at: 1132 1229 2221 2441 4034 5579 1132
Size: 97 992 220 1593 1545 2229
Fragments arranged by size:
2229 1593 1545 992 220 97
PasI CC'CwG_GG
Cuts at: 1348 2980 3311 3343 3548 1348
Size: 1632 331 32 205 4476
Fragments arranged by size:
4476 1632 331 205 32
PciI A'CATG_T
Cuts at: 2217 5575 2217
Size: 3358 3318
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2199 3295 3343 3439 1108
Size: 1091 1096 48 96 4345
Fragments arranged by size:
4345 1096 1091 96 48
PfoI T'CCnGG_A
Cuts at: 2493 2712 2924 2493
Size: 219 212 6245
Fragments arranged by size:
6245 219 212
PmeI GTTT'AAAC
Cuts at: 3980 3980
Size: 6676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4745 4777 6258 6290 4745
Size: 32 1481 32 5131
Fragments arranged by size:
5131 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2956 3071 3285 3307 3453 3587 3647 3707 3767
Size: 115 214 22 146 134 60 60 60
Cuts at: 3767 3827 3887 2956
Size: 60 60 5745
Fragments arranged by size:
5745 214 146 134 115 60 60 60
60 60 22
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6676
PsiI TTA'TAA
Cuts at: 4246 4246
Size: 6676
PspOMI G'GGCC_C
Cuts at: 2864 3035 3530 2864
Size: 171 495 6010
Fragments arranged by size:
6010 495 171
PspXI vC'TCGA_Gb
Cuts at: 2678 2915 2678
Size: 237 6439
PstI C_TGCA'G
Cuts at: 830 3595 3655 3775 3835 4028 830
Size: 2765 60 120 60 193 3478
Fragments arranged by size:
3478 2765 193 120 60 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6676
PvuII CAG'CTG
Cuts at: 2812 2812
Size: 6676
SacI G_AGCT'C
Cuts at: 2523 2523
Size: 6676
SacII CC_GC'GG
Cuts at: 2150 2150
Size: 6676
SalI G'TCGA_C
Cuts at: 1936 4018 1936
Size: 2082 4594
SanDI GG'GwC_CC
Cuts at: 2956 3071 3285 3453 2956
Size: 115 214 168 6179
Fragments arranged by size:
6179 214 168 115
SbfI CC_TGCA'GG
Cuts at: 3595 3655 3775 3835 4028 3595
Size: 60 120 60 193 6243
Fragments arranged by size:
6243 193 120 60 60
ScaI AGT'ACT
Cuts at: 4924 6636 4924
Size: 1712 4964
SfcI C'TryA_G
Cuts at: 826 1045 2293 3591 3651 3771 3831 4024 5159
Size: 219 1248 1298 60 120 60 193 1135
Cuts at: 5159 5840 6031 826
Size: 681 191 1471
Fragments arranged by size:
1471 1298 1248 1135 681 219 193 191
120 60 60
SfoI GGC'GCC
Cuts at: 2694 2694
Size: 6676
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
SgrAI Cr'CCGG_yG
Cuts at: 1700 2096 1700
Size: 396 6280
SmaI CCC'GGG
Cuts at: 1872 2953 4003 1872
Size: 1081 1050 4545
Fragments arranged by size:
4545 1081 1050
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2375 2654 2678 2915 3133 4780
Size: 197 926 432 279 24 237 218 1647
Cuts at: 4780 5681 5943 820
Size: 901 262 1553
Fragments arranged by size:
1647 1553 926 901 432 279 262 237
218 197 24
SnaBI TAC'GTA
Cuts at: 493 4467 493
Size: 3974 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6676
SphI G_CATG'C
Cuts at: 1132 2441 4034 1132
Size: 1309 1593 3774
Fragments arranged by size:
3774 1593 1309
SspI AAT'ATT
Cuts at: 5 52 4600 5
Size: 47 4548 2081
Fragments arranged by size:
4548 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6676
StyI C'CwwG_G
Cuts at: 513 1065 2004 3438 3477 3909 4115 513
Size: 552 939 1434 39 432 206 3074
Fragments arranged by size:
3074 1434 939 552 432 206 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2571 2654 2993 4703 4720 4873 5058
Size: 1255 991 83 339 1710 17 153 185
Cuts at: 5058 5503 325
Size: 445 1498
Fragments arranged by size:
1710 1498 1255 991 445 339 185 153
83 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4922 6634 96
Size: 274 80 33 51 1443 2945 1712 138
Fragments arranged by size:
2945 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3339 3371 6636 6668 3339
Size: 32 3265 32 3347
Fragments arranged by size:
3347 3265 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2188 1316
Size: 872 5804
XbaI T'CTAG_A
Cuts at: 4012 4012
Size: 6676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2430 2430
Size: 6676
XhoI C'TCGA_G
Cuts at: 1943 2678 2915 1943
Size: 735 237 5704
Fragments arranged by size:
5704 735 237
XmaI C'CCGG_G
Cuts at: 1870 2951 4001 1870
Size: 1081 1050 4545
Fragments arranged by size:
4545 1081 1050
XmnI GAAnn'nnTTC
Cuts at: 2600 4805 2600
Size: 2205 4471
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4484 276
Size: 53 83 186 936 2950 2468
Fragments arranged by size:
2950 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PsrI RsrII SapI SexAI SfiI SrfI
SwaI