Restriction map for
pFN21AE5715
(Circular) (Six-base) MAPSORT of: pFN21AE5715.seq Check: 9194 from: 1 to: 6598
pFN21AE5715 6598 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4408 278
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3941 1937
Size: 2004 4594
Acc65I G'GTAC_C
Cuts at: 3919 3919
Size: 6598
AclI AA'CG_TT
Cuts at: 1563 4725 5098 1563
Size: 3162 373 3063
Fragments arranged by size:
3162 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3186 4661 6045 6313 1632
Size: 1554 1475 1384 268 1917
Fragments arranged by size:
1917 1554 1475 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2624 2656 2831 820
Size: 197 1607 32 175 4587
Fragments arranged by size:
4587 1607 197 175 32
AflIII A'CryG_T
Cuts at: 1203 2166 5497 6474 1203
Size: 963 3331 977 1327
Fragments arranged by size:
3331 1327 977 963
AgeI A'CCGG_T
Cuts at: 3881 5429 3881
Size: 1548 5050
AhdI GACnn_n'nnGTC
Cuts at: 3456 5327 3456
Size: 1871 4727
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2748 3447 3609 1184
Size: 1564 699 162 4173
Fragments arranged by size:
4173 1564 699 162
AlwNI CAG_nnn'CTG
Cuts at: 2363 3476 4341 5913 2363
Size: 1113 865 1572 3048
Fragments arranged by size:
3048 1572 1113 865
ApaI G_GGCC'C
Cuts at: 2112 2652 2112
Size: 540 6058
ApaLI G'TGCA_C
Cuts at: 4655 5811 4655
Size: 1156 5442
ApoI r'AATT_y
Cuts at: 1493 2221 2593 2784 3907 4133 4285 6394 1493
Size: 728 372 191 1123 226 152 2109 1697
Fragments arranged by size:
2109 1697 1123 728 372 226 191 152
AseI AT'TA_AT
Cuts at: 160 1032 2334 5152 160
Size: 872 1302 2818 1606
Fragments arranged by size:
2818 1606 1302 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
AvaI C'yCGr_G
Cuts at: 1870 1943 3779 3923 1870
Size: 73 1836 144 4545
Fragments arranged by size:
4545 1836 144 73
BamHI G'GATC_C
Cuts at: 3928 4295 3928
Size: 367 6231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3308 3919 5374 618
Size: 325 217 627 1521 611 1455 1842
Fragments arranged by size:
1842 1521 1455 627 611 325 217
BanII G_rGCy'C
Cuts at: 1391 2112 2652 2772 2803 3917 1391
Size: 721 540 120 31 1114 4072
Fragments arranged by size:
4072 1114 721 540 120 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2610 928
Size: 971 711 4916
Fragments arranged by size:
4916 971 711
BbvCI CC'TCA_GC
Cuts at: 3549 3549
Size: 6598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2290 2324 4789 4823 1903
Size: 34 353 34 2465 34 3678
Fragments arranged by size:
3678 2465 353 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4490 5700 4490
Size: 1210 5388
BclI T'GATC_A
Cuts at: 1548 2142 1548
Size: 594 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5209 136
Size: 107 122 71 631 605 3537 1525
Fragments arranged by size:
3537 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6593 6593
Size: 6598
BlpI GC'TnA_GC
Cuts at: 3108 4015 3108
Size: 907 5691
Bme1580I G_kGCm'C
Cuts at: 1790 2112 2652 3183 4659 5815 1790
Size: 322 540 531 1476 1156 2573
Fragments arranged by size:
2573 1476 1156 540 531 322
BmgBI CAC'GTC
Cuts at: 1318 2068 3464 1318
Size: 750 1396 4452
Fragments arranged by size:
4452 1396 750
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3333 4329 5287
Size: 416 53 83 399 24 1916 996 958
Cuts at: 5287 442
Size: 1753
Fragments arranged by size:
1916 1753 996 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3616 3771 5258 1239
Size: 2377 155 1487 2579
Fragments arranged by size:
2579 2377 1487 155
Bpu10I CC'TnA_GC
Cuts at: 3303 3549 3303
Size: 246 6352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4723 5588 5886 4723
Size: 865 298 5435
Fragments arranged by size:
5435 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2268 3379 5261 882
Size: 617 769 1111 1882 2219
Fragments arranged by size:
2219 1882 1111 769 617
BsaAI yAC'GTr
Cuts at: 493 1204 2047 4389 493
Size: 711 843 2342 2702
Fragments arranged by size:
2702 2342 843 711
BsaBI GATnn'nnATC
Cuts at: 6592 6592
Size: 6598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4405 4787 5422 275
Size: 53 83 186 936 2872 382 635 1451
Fragments arranged by size:
2872 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2124 2934 3881 5031 5429 5703 5850 6261 2124
Size: 810 947 1150 398 274 147 411 2461
Fragments arranged by size:
2461 1150 947 810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6373
Fragments arranged by size:
6373 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 3016 3642 1186
Size: 960 870 626 4142
Fragments arranged by size:
4142 960 870 626
BseYI C'CCAG_C
Cuts at: 2012 2021 3722 5801 6297 2012
Size: 9 1701 2079 496 2313
Fragments arranged by size:
2313 2079 1701 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6025
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4048 4809 5837 1540
Size: 275 186 2047 761 1028 2301
Fragments arranged by size:
2301 2047 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2772 2803 3196 3361 4659 4744 5815 2772
Size: 31 393 165 1298 85 1071 3555
Fragments arranged by size:
3555 1298 1071 393 165 85 31
BsmI GAATG_Cn'
Cuts at: 2490 4109 4202 2490
Size: 1619 93 4886
Fragments arranged by size:
4886 1619 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2936 2936
Size: 6598
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2652 2772 2803 3183 3196 3361
Size: 399 322 540 120 31 380 13 165
Cuts at: 3361 3917 4659 4744 5815 1391
Size: 556 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 556 540 399 380 322
165 120 85 31 13
BspEI T'CCGG_A
Cuts at: 2124 2934 6261 2124
Size: 810 3327 2461
Fragments arranged by size:
3327 2461 810
BspHI T'CATG_A
Cuts at: 3213 4487 6217 3213
Size: 1274 1730 3594
Fragments arranged by size:
3594 1730 1274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3953 844
Size: 3109 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4485 1394
Size: 429 2662 3507
Fragments arranged by size:
3507 2662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5093 5267 65
Size: 1183 3845 174 1396
Fragments arranged by size:
3845 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3881 5242 5429 1700
Size: 110 2071 1361 187 2869
Fragments arranged by size:
2869 2071 1361 187 110
BsrGI T'GTAC_A
Cuts at: 96 2431 3604 96
Size: 2335 1173 3090
Fragments arranged by size:
3090 2335 1173
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6598
BssSI C'ACGA_G
Cuts at: 3408 4658 5670 3408
Size: 1250 1012 4336
Fragments arranged by size:
4336 1250 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3476 794
Size: 2682 3916
BstBI TT'CG_AA
Cuts at: 2284 5402 2284
Size: 3118 3480
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6598
BstYI r'GATC_y
Cuts at: 1972 2127 2629 3928 4295 4680 4697 6149 6235
Size: 155 502 1299 367 385 17 1452 86
Cuts at: 6235 6247 6593 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1299 502 385 367 346 155
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3515 513
Size: 552 2450 3596
Fragments arranged by size:
3596 2450 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2093 487
Size: 683 56 867 4992
Fragments arranged by size:
4992 867 683 56
BtsI GCAGTG_nn'
Cuts at: 4114 4908 4928 4114
Size: 794 20 5784
Fragments arranged by size:
5784 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6563
DraI TTT'AAA
Cuts at: 3902 3902
Size: 6598
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5605 809
Size: 507 4289 1802
Fragments arranged by size:
4289 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3078 4045 4934
Size: 54 1425 50 275 18 1248 967 889
Cuts at: 4934 6563 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1248 967 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4045 1537
Size: 275 2233 4090
Fragments arranged by size:
4090 2233 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3114 4528 1353
Size: 1761 1414 3423
Fragments arranged by size:
3423 1761 1414
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3338 5185 5559 5705 387
Size: 1064 138 1749 1847 374 146 1280
Fragments arranged by size:
1847 1749 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2770 2801 2770
Size: 31 6567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3186 3616 3771 4661 5258 6045 6313
Size: 393 1554 430 155 890 597 787 268
Cuts at: 6313 1239
Size: 1524
Fragments arranged by size:
1554 1524 890 787 597 430 393 268
155
EcoNI CCTnn'n_nnAGG
Cuts at: 3201 3201
Size: 6598
EcoO109I rG'GnC_Cy
Cuts at: 2053 2108 2053
Size: 55 6543
EcoRI G'AATT_C
Cuts at: 3907 4285 3907
Size: 378 6220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2567 2650 2567
Size: 83 6515
FspI TGC'GCA
Cuts at: 3506 5104 3506
Size: 1598 5000
FspAI rTGC'GCAy
Cuts at: 3506 3506
Size: 6598
HaeII r_GCGC'y
Cuts at: 1678 2623 3127 5745 1678
Size: 945 504 2618 2531
Fragments arranged by size:
2618 2531 945 504
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3442 3474 5239 5271 5313
Size: 32 135 32 1870 32 1765 32 42
Cuts at: 5313 5345 1373
Size: 32 2626
Fragments arranged by size:
2626 1870 1765 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3420 3871 3942 4188 669
Size: 988 281 1482 451 71 246 3079
Fragments arranged by size:
3079 1482 988 451 281 246 71
HindIII A'AGCT_T
Cuts at: 748 2713 748
Size: 1965 4633
HpaI GTT'AAC
Cuts at: 3871 4188 3871
Size: 317 6281
KpnI G_GTAC'C
Cuts at: 3923 3923
Size: 6598
MfeI C'AATT_G
Cuts at: 4197 4197
Size: 6598
MluI A'CGCG_T
Cuts at: 2166 6474 2166
Size: 4308 2290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2184 2494 3726 3914 5712 5896 1305
Size: 879 310 1232 188 1798 184 2007
Fragments arranged by size:
2007 1798 1232 879 310 188 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3080 10
Size: 54 1425 343 1248 3528
Fragments arranged by size:
3528 1425 1248 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2730 3561 3737 4556 4915 5074 518
Size: 648 1564 831 176 819 359 159 2042
Fragments arranged by size:
2042 1564 831 819 648 359 176 159
MspA1I CmG'CkG
Cuts at: 1606 2151 2456 3221 3473 4014 4691 5839 6084
Size: 545 305 765 252 541 677 1148 245
Cuts at: 6084 1606
Size: 2120
Fragments arranged by size:
2120 1148 765 677 545 541 305 252
245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6046
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6598
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6598
NotI GC'GGCC_GC
Cuts at: 4045 4045
Size: 6598
NspI r_CATG'y
Cuts at: 1132 1229 2207 3742 3956 5501 1132
Size: 97 978 1535 214 1545 2229
Fragments arranged by size:
2229 1545 1535 978 214 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6598
PciI A'CATG_T
Cuts at: 5497 5497
Size: 6598
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6598
PmeI GTTT'AAAC
Cuts at: 3902 3902
Size: 6598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4667 4699 6180 6212 4667
Size: 32 1481 32 5053
Fragments arranged by size:
5053 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2053 2053
Size: 6598
PshAI GACnn'nnGTC
Cuts at: 1613 3221 1613
Size: 1608 4990
PsiI TTA'TAA
Cuts at: 2234 4168 2234
Size: 1934 4664
PspOMI G'GGCC_C
Cuts at: 2108 2648 2108
Size: 540 6058
PspXI vC'TCGA_Gb
Cuts at: 3779 3779
Size: 6598
PstI C_TGCA'G
Cuts at: 830 2135 3472 3490 3950 830
Size: 1305 1337 18 460 3478
Fragments arranged by size:
3478 1337 1305 460 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6598
PvuII CAG'CTG
Cuts at: 2456 3221 3473 2456
Size: 765 252 5581
Fragments arranged by size:
5581 765 252
SacI G_AGCT'C
Cuts at: 2772 2803 2772
Size: 31 6567
SalI G'TCGA_C
Cuts at: 1936 3940 1936
Size: 2004 4594
SapI GCTCTTCn'nnn_
Cuts at: 3114 3114
Size: 6598
SbfI CC_TGCA'GG
Cuts at: 3950 3950
Size: 6598
ScaI AGT'ACT
Cuts at: 3379 4846 6558 3379
Size: 1467 1712 3419
Fragments arranged by size:
3419 1712 1467
SexAI A'CCwGG_T
Cuts at: 2877 2877
Size: 6598
SfcI C'TryA_G
Cuts at: 826 1045 2131 2745 3468 3486 3819 3946 5081
Size: 219 1086 614 723 18 333 127 1135
Cuts at: 5081 5762 5953 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1086 723 681 614 333 219
191 127 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6598
SmaI CCC'GGG
Cuts at: 1872 3925 1872
Size: 2053 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2624 2656 2831 3779 4702 5603
Size: 197 926 681 32 175 948 923 901
Cuts at: 5603 5865 820
Size: 262 1553
Fragments arranged by size:
1553 948 926 923 901 681 262 197
175 32
SnaBI TAC'GTA
Cuts at: 493 4389 493
Size: 3896 2702
SpeI A'CTAG_T
Cuts at: 152 2966 152
Size: 2814 3784
SphI G_CATG'C
Cuts at: 1132 2207 3956 1132
Size: 1075 1749 3774
Fragments arranged by size:
3774 1749 1075
SspI AAT'ATT
Cuts at: 5 52 4522 5
Size: 47 4470 2081
Fragments arranged by size:
4470 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6598
StyI C'CwwG_G
Cuts at: 513 1065 2415 4037 513
Size: 552 1350 1622 3074
Fragments arranged by size:
3074 1622 1350 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2690 4625 4642 4795 4980 5425 325
Size: 1255 1110 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1255 1110 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2431 3377 3604
Size: 274 80 33 51 1443 454 946 227
Cuts at: 3604 3826 4844 6556 96
Size: 222 1018 1712 138
Fragments arranged by size:
1712 1443 1018 946 454 274 227 222
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6558 6590 6558
Size: 32 6566
Tth111I GACn'n_nGTC
Cuts at: 1316 3285 1316
Size: 1969 4629
XbaI T'CTAG_A
Cuts at: 3934 3934
Size: 6598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3291 3291
Size: 6598
XhoI C'TCGA_G
Cuts at: 1943 3779 1943
Size: 1836 4762
XmaI C'CCGG_G
Cuts at: 1870 3923 1870
Size: 2053 4545
XmnI GAAnn'nnTTC
Cuts at: 4727 4727
Size: 6598
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4406 276
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI