Restriction map for
pFN21AE5712
(Circular) (Six-base) MAPSORT of: pFN21AE5712.seq Check: 1952 from: 1 to: 6901
pFN21AE5712 6901 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2556 4711 278
Size: 53 83 186 936 1020 2155 2468
Fragments arranged by size:
2468 2155 1020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2949 3014 4244 1937
Size: 1012 65 1230 4594
Fragments arranged by size:
4594 1230 1012 65
Acc65I G'GTAC_C
Cuts at: 4222 4222
Size: 6901
AclI AA'CG_TT
Cuts at: 1563 5028 5401 1563
Size: 3465 373 3063
Fragments arranged by size:
3465 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2283 2606 4964 6348 6616 1632
Size: 651 323 2358 1384 268 1917
Fragments arranged by size:
2358 1917 1384 651 323 268
AfeI AGC'GCT
Cuts at: 2415 2415
Size: 6901
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6704
AflIII A'CryG_T
Cuts at: 1203 5800 6777 1203
Size: 4597 977 1327
Fragments arranged by size:
4597 1327 977
AgeI A'CCGG_T
Cuts at: 5732 5732
Size: 6901
AhdI GACnn_n'nnGTC
Cuts at: 5630 5630
Size: 6901
AleI CACnn'nnGTG
Cuts at: 1166 3185 1166
Size: 2019 4882
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2687 3110 3419 1184
Size: 1503 423 309 4666
Fragments arranged by size:
4666 1503 423 309
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3699 3731 3699
Size: 32 6869
AlwNI CAG_nnn'CTG
Cuts at: 2020 2246 2365 3166 3822 3924 4644 6216 2020
Size: 226 119 801 656 102 720 1572 2705
Fragments arranged by size:
2705 1572 801 720 656 226 119 102
ApaI G_GGCC'C
Cuts at: 2102 2345 2761 3534 2102
Size: 243 416 773 5469
Fragments arranged by size:
5469 773 416 243
ApaLI G'TGCA_C
Cuts at: 2141 4958 6114 2141
Size: 2817 1156 2928
Fragments arranged by size:
2928 2817 1156
ApoI r'AATT_y
Cuts at: 1493 4210 4436 4588 6697 1493
Size: 2717 226 152 2109 1697
Fragments arranged by size:
2717 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5455 160
Size: 872 4423 1606
Fragments arranged by size:
4423 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6901
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2268 4226 1870
Size: 73 90 235 1958 4545
Fragments arranged by size:
4545 1958 235 90 73
BamHI G'GATC_C
Cuts at: 4231 4598 4231
Size: 367 6534
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 4222 5677 618
Size: 325 217 627 516 1919 1455 1842
Fragments arranged by size:
1919 1842 1455 627 516 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 2102 2345 2761 2805 2886 2930 3375
Size: 643 68 243 416 44 81 44 445
Cuts at: 3375 3534 3869 4220 1391
Size: 159 335 351 4072
Fragments arranged by size:
4072 643 445 416 351 335 243 159
81 68 44 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2640 4001 928
Size: 971 741 1361 3828
Fragments arranged by size:
3828 1361 971 741
BbvCI CC'TCA_GC
Cuts at: 3938 3938
Size: 6901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5092 5126 1903
Size: 34 3155 34 3678
Fragments arranged by size:
3678 3155 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4793 6003 4793
Size: 1210 5691
BclI T'GATC_A
Cuts at: 1548 3843 1548
Size: 2295 4606
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3827 4096 5512
Size: 107 122 71 631 605 2155 269 1416
Cuts at: 5512 136
Size: 1525
Fragments arranged by size:
2155 1525 1416 631 605 269 122 107
71
BglII A'GATC_T
Cuts at: 6896 6896
Size: 6901
BlpI GC'TnA_GC
Cuts at: 2024 2081 2282 2924 3147 4318 2024
Size: 57 201 642 223 1171 4607
Fragments arranged by size:
4607 1171 642 223 201 57
Bme1580I G_kGCm'C
Cuts at: 1790 2102 2145 2345 2761 3194 3507 3534 4041
Size: 312 43 200 416 433 313 27 507
Cuts at: 4041 4072 4962 6118 1790
Size: 31 890 1156 2573
Fragments arranged by size:
2573 1156 890 507 433 416 313 312
200 43 31 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6901
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2647 3400 4632
Size: 416 53 83 399 24 1230 753 1232
Cuts at: 4632 5590 442
Size: 958 1753
Fragments arranged by size:
1753 1232 1230 958 753 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6901
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2905 3088 2905
Size: 183 6718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2352 2387 2438 2784 3234 3380 3471 3701
Size: 1113 35 51 346 450 146 91 230
Cuts at: 3701 5561 1239
Size: 1860 2579
Fragments arranged by size:
2579 1860 1113 450 346 230 146 91
51 35
Bpu10I CC'TnA_GC
Cuts at: 2063 3938 2063
Size: 1875 5026
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 2735 4058 5026 5891 6189 2378
Size: 357 1323 968 865 298 3090
Fragments arranged by size:
3090 1323 968 865 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 3803 5564 882
Size: 617 759 1545 1761 2219
Fragments arranged by size:
2219 1761 1545 759 617
BsaAI yAC'GTr
Cuts at: 493 1204 2857 4692 493
Size: 711 1653 1835 2702
Fragments arranged by size:
2702 1835 1653 711
BsaBI GATnn'nnATC
Cuts at: 3848 6895 3848
Size: 3047 3854
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2553 4708 5090 5725
Size: 53 83 186 936 1020 2155 382 635
Cuts at: 5725 275
Size: 1451
Fragments arranged by size:
2155 1451 1020 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2133 2385 2808 5334 5732 6006 6153 6564 2133
Size: 252 423 2526 398 274 147 411 2470
Fragments arranged by size:
2526 2470 423 411 398 274 252 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3699 3729 3839 3869 4133
Size: 30 165 30 2296 30 110 30 264
Cuts at: 4133 4163 1178
Size: 30 3916
Fragments arranged by size:
3916 2296 264 165 110 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2876 3377 1186
Size: 865 825 501 4710
Fragments arranged by size:
4710 865 825 501
BseYI C'CCAG_C
Cuts at: 3058 3284 3568 6104 6600 3058
Size: 226 284 2536 496 3359
Fragments arranged by size:
3359 2536 496 284 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3455 1163
Size: 573 1719 4609
Fragments arranged by size:
4609 1719 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4351 5112 6140 1540
Size: 275 186 2350 761 1028 2301
Fragments arranged by size:
2350 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2676 2886 3717 4176 4962 5047 6118 2145
Size: 531 210 831 459 786 85 1071 2928
Fragments arranged by size:
2928 1071 831 786 531 459 210 85
BsmI GAATG_Cn'
Cuts at: 4171 4412 4505 4171
Size: 241 93 6567
Fragments arranged by size:
6567 241 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2102 2145 2345 2676 2761 2805
Size: 399 244 68 43 200 331 85 44
Cuts at: 2805 2886 2930 3194 3375 3507 3534 3717 3869
Size: 81 44 264 181 132 27 183 152
Cuts at: 3869 4041 4072 4176 4220 4962 5047 6118 1391
Size: 172 31 104 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 331 264 244 200
183 181 172 152 132 104 85 85
81 68 44 44 44 43 31 27
BspEI T'CCGG_A
Cuts at: 6564 6564
Size: 6901
BspHI T'CATG_A
Cuts at: 4161 4790 6520 4161
Size: 629 1730 4542
Fragments arranged by size:
4542 1730 629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2254 2829 2961 4256 844
Size: 1410 575 132 1295 3489
Fragments arranged by size:
3489 1410 1295 575 132
BsrBI CCG'CTC
Cuts at: 1394 1823 4788 1394
Size: 429 2965 3507
Fragments arranged by size:
3507 2965 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3670 5396 5570 65
Size: 1183 2422 1726 174 1396
Fragments arranged by size:
2422 1726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3156 4029 5545 5732 1700
Size: 110 1346 873 1516 187 2869
Fragments arranged by size:
2869 1516 1346 873 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6901
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6901
BssSI C'ACGA_G
Cuts at: 4961 5973 4961
Size: 1012 5889
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2246 3924 794
Size: 1452 1678 3771
Fragments arranged by size:
3771 1678 1452
BstBI TT'CG_AA
Cuts at: 5705 5705
Size: 6901
BstEII G'GTnAC_C
Cuts at: 1184 2107 3022 1184
Size: 923 915 5063
Fragments arranged by size:
5063 923 915
BstXI CCAn_nnnn'nTGG
Cuts at: 2548 3794 2548
Size: 1246 5655
BstYI r'GATC_y
Cuts at: 1972 3078 4231 4598 4983 5000 6452 6538 6550
Size: 1106 1153 367 385 17 1452 86 12
Cuts at: 6550 6896 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1153 1106 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3015 3015
Size: 6901
Bsu36I CC'TnA_GG
Cuts at: 3987 3987
Size: 6901
BtgI C'CryG_G
Cuts at: 513 1065 2004 3507 3523 3624 3766 513
Size: 552 939 1503 16 101 142 3648
Fragments arranged by size:
3648 1503 939 552 142 101 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2580 3835 487
Size: 683 56 1354 1255 3553
Fragments arranged by size:
3553 1354 1255 683 56
BtsI GCAGTG_nn'
Cuts at: 3549 3708 3925 4417 5211 5231 3549
Size: 159 217 492 794 20 5219
Fragments arranged by size:
5219 794 492 217 159 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6901
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3613 3648 3726 3761 529
Size: 35 3049 35 78 35 3669
Fragments arranged by size:
3669 3049 78 35 35 35
DraI TTT'AAA
Cuts at: 4205 4205
Size: 6901
DraIII CAC_nnn'GTG
Cuts at: 2151 2151
Size: 6901
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5908 809
Size: 507 4592 1802
Fragments arranged by size:
4592 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 3634 4348
Size: 54 1425 50 275 18 295 1509 714
Cuts at: 4348 5237 6866 8
Size: 889 1629 43
Fragments arranged by size:
1629 1509 1425 889 714 295 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4348 1537
Size: 275 2536 4090
Fragments arranged by size:
4090 2536 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2346 2917 4831 1353
Size: 993 571 1914 3423
Fragments arranged by size:
3423 1914 993 571
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5488 5862 6008 387
Size: 1064 138 3899 374 146 1280
Fragments arranged by size:
3899 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2884 2884
Size: 6901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2352 2387 2438 2606 2784 3234
Size: 393 651 69 35 51 168 178 450
Cuts at: 3234 3380 3471 3701 4964 5561 6348 6616 1239
Size: 146 91 230 1263 597 787 268 1524
Fragments arranged by size:
1524 1263 787 651 597 450 393 268
230 178 168 146 91 69 51 35
EcoNI CCTnn'n_nnAGG
Cuts at: 3324 3362 3324
Size: 38 6863
EcoO109I rG'GnC_Cy
Cuts at: 2059 2164 2243 2342 2722 2758 3163 3256 3530
Size: 105 79 99 380 36 405 93 274
Cuts at: 3530 3531 3819 2059
Size: 1 288 5141
Fragments arranged by size:
5141 405 380 288 274 105 99 93
79 36 1
EcoRI G'AATT_C
Cuts at: 4210 4588 4210
Size: 378 6523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3607 3607
Size: 6901
FspI TGC'GCA
Cuts at: 2324 5407 2324
Size: 3083 3818
HaeII r_GCGC'y
Cuts at: 1678 2088 2417 2515 6048 1678
Size: 410 329 98 3533 2531
Fragments arranged by size:
3533 2531 410 329 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 2720 2752 2962
Size: 32 135 32 638 32 478 32 210
Cuts at: 2962 2994 4133 4165 5542 5574 5616 5648 1373
Size: 32 1139 32 1377 32 42 32 2626
Fragments arranged by size:
2626 1377 1139 638 478 210 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4245 4491 669
Size: 988 281 2307 246 3079
Fragments arranged by size:
3079 2307 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6901
HpaI GTT'AAC
Cuts at: 4491 4491
Size: 6901
KpnI G_GTAC'C
Cuts at: 4226 4226
Size: 6901
MfeI C'AATT_G
Cuts at: 3246 4500 3246
Size: 1254 5647
MluI A'CGCG_T
Cuts at: 6777 6777
Size: 6901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3320 6015 6199 1305
Size: 2015 2695 184 2007
Fragments arranged by size:
2695 2015 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3636 10
Size: 54 1425 343 1804 3275
Fragments arranged by size:
3275 1804 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2669 2708 3185 3401 3678 3732 4859
Size: 648 1503 39 477 216 277 54 1127
Cuts at: 4859 5218 5377 518
Size: 359 159 2042
Fragments arranged by size:
2042 1503 1127 648 477 359 277 216
159 54 39
MspA1I CmG'CkG
Cuts at: 1606 2995 3058 3116 3212 4317 4994 6142 6387
Size: 1389 63 58 96 1105 677 1148 245
Cuts at: 6387 1606
Size: 2120
Fragments arranged by size:
2120 1389 1148 1105 677 245 96 63
58
NaeI GCC'GGC
Cuts at: 3158 3158
Size: 6901
NcoI C'CATG_G
Cuts at: 513 1065 2004 3507 3766 513
Size: 552 939 1503 259 3648
Fragments arranged by size:
3648 1503 939 552 259
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6901
NgoMIV G'CCGG_C
Cuts at: 3156 3156
Size: 6901
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6901
NotI GC'GGCC_GC
Cuts at: 4348 4348
Size: 6901
NspI r_CATG'y
Cuts at: 1132 1229 4259 5804 1132
Size: 97 3030 1545 2229
Fragments arranged by size:
3030 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2345 1348
Size: 997 5904
PciI A'CATG_T
Cuts at: 5800 5800
Size: 6901
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2206 3130 1108
Size: 1098 924 4879
Fragments arranged by size:
4879 1098 924
PfoI T'CCnGG_A
Cuts at: 2364 3717 2364
Size: 1353 5548
PmeI GTTT'AAAC
Cuts at: 4205 4205
Size: 6901
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2345 2377 3699 3731 4970 5002 6483 6515 2345
Size: 32 1322 32 1239 32 1481 32 2731
Fragments arranged by size:
2731 1481 1322 1239 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2243 2722 3163 3256 2164
Size: 79 479 441 93 5809
Fragments arranged by size:
5809 479 441 93 79
PshAI GACnn'nnGTC
Cuts at: 1613 2937 1613
Size: 1324 5577
PsiI TTA'TAA
Cuts at: 4471 4471
Size: 6901
PspOMI G'GGCC_C
Cuts at: 2098 2341 2757 3530 2098
Size: 243 416 773 5469
Fragments arranged by size:
5469 773 416 243
PstI C_TGCA'G
Cuts at: 830 2251 2958 3089 3328 3474 4253 830
Size: 1421 707 131 239 146 779 3478
Fragments arranged by size:
3478 1421 779 707 239 146 131
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6901
PvuII CAG'CTG
Cuts at: 2995 3058 3116 3212 2995
Size: 63 58 96 6684
Fragments arranged by size:
6684 96 63 58
SacI G_AGCT'C
Cuts at: 2886 2886
Size: 6901
SalI G'TCGA_C
Cuts at: 1936 4243 1936
Size: 2307 4594
SanDI GG'GwC_CC
Cuts at: 2722 2722
Size: 6901
SbfI CC_TGCA'GG
Cuts at: 2251 4253 2251
Size: 2002 4899
ScaI AGT'ACT
Cuts at: 5149 6861 5149
Size: 1712 5189
SexAI A'CCwGG_T
Cuts at: 2205 2205
Size: 6901
SfcI C'TryA_G
Cuts at: 826 1045 2247 2954 3085 3324 3407 3470 3814
Size: 219 1202 707 131 239 83 63 344
Cuts at: 3814 4249 5384 6065 6256 826
Size: 435 1135 681 191 1471
Fragments arranged by size:
1471 1202 1135 707 681 435 344 239
219 191 131 83 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 3827 3827
Size: 6901
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6901
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6901
SmaI CCC'GGG
Cuts at: 1872 2270 4228 1872
Size: 398 1958 4545
Fragments arranged by size:
4545 1958 398
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 2714 4073 5005 5906 6168
Size: 197 926 450 321 1359 932 901 262
Cuts at: 6168 820
Size: 1553
Fragments arranged by size:
1553 1359 932 926 901 450 321 262
197
SnaBI TAC'GTA
Cuts at: 493 4692 493
Size: 4199 2702
SpeI A'CTAG_T
Cuts at: 152 3604 152
Size: 3452 3449
SphI G_CATG'C
Cuts at: 1132 4259 1132
Size: 3127 3774
SspI AAT'ATT
Cuts at: 5 52 4825 5
Size: 47 4773 2081
Fragments arranged by size:
4773 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6901
StyI C'CwwG_G
Cuts at: 513 1065 2004 2517 2547 3018 3507 3766 4340
Size: 552 939 513 30 471 489 259 574
Cuts at: 4340 513
Size: 3074
Fragments arranged by size:
3074 939 574 552 513 489 471 259
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3197 3298 3340 3746 4136 4928 4945
Size: 1255 1617 101 42 406 390 792 17
Cuts at: 4945 5098 5283 5728 325
Size: 153 185 445 1498
Fragments arranged by size:
1617 1498 1255 792 445 406 390 185
153 101 42 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5147 6859 96
Size: 274 80 33 51 1443 3170 1712 138
Fragments arranged by size:
3170 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6861 6893 6861
Size: 32 6869
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6901
XbaI T'CTAG_A
Cuts at: 4237 4237
Size: 6901
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6901
XmaI C'CCGG_G
Cuts at: 1870 2268 4226 1870
Size: 398 1958 4545
Fragments arranged by size:
4545 1958 398
XmnI GAAnn'nnTTC
Cuts at: 2360 5030 2360
Size: 2670 4231
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2554 4709 276
Size: 53 83 186 936 1020 2155 2468
Fragments arranged by size:
2468 2155 1020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SapI SfoI SrfI
SwaI XcmI