Restriction map for
pFN21AE5711
(Circular) (Six-base) MAPSORT of: pFN21AE5711.seq Check: 3090 from: 1 to: 7120
pFN21AE5711 7120 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4930 278
Size: 53 83 186 936 3394 2468
Fragments arranged by size:
3394 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2350 2682 2925 4463 1937
Size: 413 332 243 1538 4594
Fragments arranged by size:
4594 1538 413 332 243
Acc65I G'GTAC_C
Cuts at: 4441 4441
Size: 7120
AclI AA'CG_TT
Cuts at: 1563 4406 5247 5620 1563
Size: 2843 841 373 3063
Fragments arranged by size:
3063 2843 841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2184 2852 5183 6567 6835 1632
Size: 552 668 2331 1384 268 1917
Fragments arranged by size:
2331 1917 1384 668 552 268
AflII C'TTAA_G
Cuts at: 820 1017 3877 820
Size: 197 2860 4063
Fragments arranged by size:
4063 2860 197
AflIII A'CryG_T
Cuts at: 1203 3273 3666 6019 6996 1203
Size: 2070 393 2353 977 1327
Fragments arranged by size:
2353 2070 1327 977 393
AgeI A'CCGG_T
Cuts at: 5951 5951
Size: 7120
AhdI GACnn_n'nnGTC
Cuts at: 5849 5849
Size: 7120
AleI CACnn'nnGTG
Cuts at: 1166 2396 2900 1166
Size: 1230 504 5386
Fragments arranged by size:
5386 1230 504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7120
AlwNI CAG_nnn'CTG
Cuts at: 2038 2211 2435 2542 3045 3159 3997 4088 4863
Size: 173 224 107 503 114 838 91 775
Cuts at: 4863 6435 2038
Size: 1572 2723
Fragments arranged by size:
2723 1572 838 775 503 224 173 114
107 91
ApaI G_GGCC'C
Cuts at: 2495 2721 2866 3617 2495
Size: 226 145 751 5998
Fragments arranged by size:
5998 751 226 145
ApaLI G'TGCA_C
Cuts at: 5177 6333 5177
Size: 1156 5964
ApoI r'AATT_y
Cuts at: 1493 4121 4429 4655 4807 6916 1493
Size: 2628 308 226 152 2109 1697
Fragments arranged by size:
2628 2109 1697 308 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5674 160
Size: 872 4642 1606
Fragments arranged by size:
4642 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7120
AvaI C'yCGr_G
Cuts at: 1870 1943 2898 3230 3566 4445 1870
Size: 73 955 332 336 879 4545
Fragments arranged by size:
4545 955 879 336 332 73
BamHI G'GATC_C
Cuts at: 2831 4450 4817 2831
Size: 1619 367 5134
Fragments arranged by size:
5134 1619 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2990 4441 5896 618
Size: 325 217 627 1203 1451 1455 1842
Fragments arranged by size:
1842 1455 1451 1203 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2412 2495 2721 2866 3126 3617 3918 4439
Size: 1021 83 226 145 260 491 301 521
Cuts at: 4439 1391
Size: 4072
Fragments arranged by size:
4072 1021 521 491 301 260 226 145
83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2652 3079 3631 3640 928
Size: 971 753 427 552 9 4408
Fragments arranged by size:
4408 971 753 552 427 9
BbvCI CC'TCA_GC
Cuts at: 3149 3149
Size: 7120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5311 5345 1903
Size: 34 3374 34 3678
Fragments arranged by size:
3678 3374 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5012 6222 5012
Size: 1210 5910
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7120
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4068 5731 136
Size: 107 122 71 631 605 2396 1663 1525
Fragments arranged by size:
2396 1663 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7115 7115
Size: 7120
BlpI GC'TnA_GC
Cuts at: 4537 4537
Size: 7120
Bme1580I G_kGCm'C
Cuts at: 1790 2495 2721 2866 2897 3617 5181 6337 1790
Size: 705 226 145 31 720 1564 1156 2573
Fragments arranged by size:
2573 1564 1156 720 705 226 145 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7120
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2788 3055 3896
Size: 416 53 83 399 24 1371 267 841
Cuts at: 3896 4851 5809 442
Size: 955 958 1753
Fragments arranged by size:
1753 1371 958 955 841 416 399 267
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2110 2126 2359 2457 2673 3140 4019 4175
Size: 871 16 233 98 216 467 879 156
Cuts at: 4175 4238 5780 1239
Size: 63 1542 2579
Fragments arranged by size:
2579 1542 879 871 467 233 216 156
98 63 16
Bpu10I CC'TnA_GC
Cuts at: 3149 3149
Size: 7120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5245 6110 6408 5245
Size: 865 298 5957
Fragments arranged by size:
5957 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5783 882
Size: 617 4284 2219
Fragments arranged by size:
4284 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3276 4911 493
Size: 711 2072 1635 2702
Fragments arranged by size:
2702 2072 1635 711
BsaBI GATnn'nnATC
Cuts at: 3951 7114 3951
Size: 3163 3957
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4927 5309 5944 275
Size: 53 83 186 936 3394 382 635 1451
Fragments arranged by size:
3394 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3555 5553 5951 6225 6372 6783 3555
Size: 1998 398 274 147 411 3892
Fragments arranged by size:
3892 1998 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2864 2894 3839 3869 4092
Size: 30 165 30 1461 30 945 30 223
Cuts at: 4092 4122 1178
Size: 30 4176
Fragments arranged by size:
4176 1461 945 223 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3394 3725 4388 4393 4429 1186
Size: 2208 331 663 5 36 3877
Fragments arranged by size:
3877 2208 663 331 36 5
BseYI C'CCAG_C
Cuts at: 2047 2587 3396 4104 4291 6323 6819 2047
Size: 540 809 708 187 2032 496 2348
Fragments arranged by size:
2348 2032 809 708 540 496 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2101 2868 3839 1163
Size: 573 365 767 971 4444
Fragments arranged by size:
4444 971 767 573 365
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4570 5331 6359 1540
Size: 275 186 2569 761 1028 2301
Fragments arranged by size:
2569 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2412 3126 3660 3918 5181 5266 6337 2412
Size: 714 534 258 1263 85 1071 3195
Fragments arranged by size:
3195 1263 1071 714 534 258 85
BsmI GAATG_Cn'
Cuts at: 2014 3334 4631 4724 2014
Size: 1320 1297 93 4410
Fragments arranged by size:
4410 1320 1297 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2174 2174
Size: 7120
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2412 2495 2721 2866 2897 3126 3617
Size: 399 622 83 226 145 31 229 491
Cuts at: 3617 3660 3918 4439 5181 5266 6337 1391
Size: 43 258 521 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 622 521 491 399 258
229 226 145 85 83 43 31
BspEI T'CCGG_A
Cuts at: 3555 6783 3555
Size: 3228 3892
BspHI T'CATG_A
Cuts at: 5009 6739 5009
Size: 1730 5390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3501 4475 844
Size: 2657 974 3489
Fragments arranged by size:
3489 2657 974
BsrBI CCG'CTC
Cuts at: 1394 1823 5007 1394
Size: 429 3184 3507
Fragments arranged by size:
3507 3184 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2629 5615 5789 65
Size: 1183 1381 2986 174 1396
Fragments arranged by size:
2986 1396 1381 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3135 5764 5951 1700
Size: 110 1325 2629 187 2869
Fragments arranged by size:
2869 2629 1325 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7120
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7120
BssSI C'ACGA_G
Cuts at: 5180 6192 5180
Size: 1012 6108
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2038 794
Size: 1244 5876
BstBI TT'CG_AA
Cuts at: 5924 5924
Size: 7120
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7120
BstXI CCAn_nnnn'nTGG
Cuts at: 4311 4311
Size: 7120
BstYI r'GATC_y
Cuts at: 1972 2831 3114 3849 4450 4817 5202 5219 6671
Size: 859 283 735 601 367 385 17 1452
Cuts at: 6671 6757 6769 7115 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 859 735 601 385 367 346
283 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2025 2306 2957 2025
Size: 281 651 6188
Fragments arranged by size:
6188 651 281
BtgI C'CryG_G
Cuts at: 513 1065 2548 4390 513
Size: 552 1483 1842 3243
Fragments arranged by size:
3243 1842 1483 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6381
Fragments arranged by size:
6381 683 56
BtsI GCAGTG_nn'
Cuts at: 4636 5430 5450 4636
Size: 794 20 6306
Fragments arranged by size:
6306 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7085
DraI TTT'AAA
Cuts at: 4424 4424
Size: 7120
DraIII CAC_nnn'GTG
Cuts at: 2736 2736
Size: 7120
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2200 2438 2921 3179 6127 809
Size: 507 884 238 483 258 2948 1802
Fragments arranged by size:
2948 1802 884 507 483 258 238
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3675 3921 4567
Size: 54 1425 50 275 18 1845 246 646
Cuts at: 4567 5456 7085 8
Size: 889 1629 43
Fragments arranged by size:
1845 1629 1425 889 646 275 246 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4567 1537
Size: 275 2755 4090
Fragments arranged by size:
4090 2755 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2417 3105 3347 3999 4353 5050 1353
Size: 1064 688 242 652 354 697 3423
Fragments arranged by size:
3423 1064 697 688 652 354 242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2483 5707 6081 6227 387
Size: 1064 138 894 3224 374 146 1280
Fragments arranged by size:
3224 1280 1064 894 374 146 138
EcoICRI GAG'CTC
Cuts at: 2410 3124 3916 2410
Size: 714 792 5614
Fragments arranged by size:
5614 792 714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2126 2184 2359 2457 2673 2852
Size: 393 478 16 58 175 98 216 179
Cuts at: 2852 3140 4019 4175 4238 5183 5780 6567 6835
Size: 288 879 156 63 945 597 787 268
Cuts at: 6835 1239
Size: 1524
Fragments arranged by size:
1524 945 879 787 597 478 393 288
268 216 179 175 156 98 63 58
16
EcoNI CCTnn'n_nnAGG
Cuts at: 2870 3758 2870
Size: 888 6232
EcoO109I rG'GnC_Cy
Cuts at: 2135 2193 2491 2718 2863 3504 3614 3826 3994
Size: 58 298 227 145 641 110 212 168
Cuts at: 3994 2135
Size: 5261
Fragments arranged by size:
5261 641 298 227 212 168 145 110
58
EcoRI G'AATT_C
Cuts at: 4121 4429 4807 4121
Size: 308 378 6434
Fragments arranged by size:
6434 378 308
FspI TGC'GCA
Cuts at: 5626 5626
Size: 7120
HaeII r_GCGC'y
Cuts at: 1678 6267 1678
Size: 4589 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2669 2701 2979 3011 5761
Size: 32 135 32 1097 32 278 32 2750
Cuts at: 5761 5793 5835 5867 1373
Size: 32 42 32 2626
Fragments arranged by size:
2750 2626 1097 278 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3337 4464 4710 669
Size: 988 281 1399 1127 246 3079
Fragments arranged by size:
3079 1399 1127 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7120
HpaI GTT'AAC
Cuts at: 4710 4710
Size: 7120
KpnI G_GTAC'C
Cuts at: 4445 4445
Size: 7120
MfeI C'AATT_G
Cuts at: 4719 4719
Size: 7120
MluI A'CGCG_T
Cuts at: 6996 6996
Size: 7120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4427 6234 6418 1305
Size: 3122 1807 184 2007
Fragments arranged by size:
3122 2007 1807 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3677 10
Size: 54 1425 343 1845 3453
Fragments arranged by size:
3453 1845 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 2900 5078 5437 5596 518
Size: 648 1230 504 2178 359 159 2042
Fragments arranged by size:
2178 2042 1230 648 504 359 159
MspA1I CmG'CkG
Cuts at: 1606 2208 2273 2802 3420 4153 4295 4536 5213
Size: 602 65 529 618 733 142 241 677
Cuts at: 5213 6361 6606 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 733 677 618 602 529 245
241 142 65
NcoI C'CATG_G
Cuts at: 513 1065 2548 513
Size: 552 1483 5085
Fragments arranged by size:
5085 1483 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7120
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7120
NotI GC'GGCC_GC
Cuts at: 4567 4567
Size: 7120
NspI r_CATG'y
Cuts at: 1132 1229 2584 3670 4132 4478 6023 1132
Size: 97 1355 1086 462 346 1545 2229
Fragments arranged by size:
2229 1545 1355 1086 462 346 97
PasI CC'CwG_GG
Cuts at: 1348 2488 4311 1348
Size: 1140 1823 4157
Fragments arranged by size:
4157 1823 1140
PciI A'CATG_T
Cuts at: 3666 6019 3666
Size: 2353 4767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2542 1108
Size: 1434 5686
PfoI T'CCnGG_A
Cuts at: 2652 2827 3117 4164 2652
Size: 175 290 1047 5608
Fragments arranged by size:
5608 1047 290 175
PmeI GTTT'AAAC
Cuts at: 4424 4424
Size: 7120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5189 5221 6702 6734 5189
Size: 32 1481 32 5575
Fragments arranged by size:
5575 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2193 3504 3826 3994 2193
Size: 1311 322 168 5319
Fragments arranged by size:
5319 1311 322 168
PshAI GACnn'nnGTC
Cuts at: 1613 2346 1613
Size: 733 6387
PsiI TTA'TAA
Cuts at: 4690 4690
Size: 7120
PspOMI G'GGCC_C
Cuts at: 2491 2717 2862 3613 2491
Size: 226 145 751 5998
Fragments arranged by size:
5998 751 226 145
PspXI vC'TCGA_Gb
Cuts at: 2898 3230 2898
Size: 332 6788
PstI C_TGCA'G
Cuts at: 830 3264 3834 4152 4472 830
Size: 2434 570 318 320 3478
Fragments arranged by size:
3478 2434 570 320 318
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7120
PvuII CAG'CTG
Cuts at: 2208 2273 4153 4295 2208
Size: 65 1880 142 5033
Fragments arranged by size:
5033 1880 142 65
SacI G_AGCT'C
Cuts at: 2412 3126 3918 2412
Size: 714 792 5614
Fragments arranged by size:
5614 792 714
SalI G'TCGA_C
Cuts at: 1936 4462 1936
Size: 2526 4594
SanDI GG'GwC_CC
Cuts at: 3504 3504
Size: 7120
SapI GCTCTTCn'nnn_
Cuts at: 2417 2417
Size: 7120
SbfI CC_TGCA'GG
Cuts at: 3834 4472 3834
Size: 638 6482
ScaI AGT'ACT
Cuts at: 5368 7080 5368
Size: 1712 5408
SexAI A'CCwGG_T
Cuts at: 3174 3174
Size: 7120
SfcI C'TryA_G
Cuts at: 826 1045 2077 2797 2982 3260 3830 4148 4194
Size: 219 1032 720 185 278 570 318 46
Cuts at: 4194 4468 5603 6284 6475 826
Size: 274 1135 681 191 1471
Fragments arranged by size:
1471 1135 1032 720 681 570 318 278
274 219 191 185 46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7120
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7120
SmaI CCC'GGG
Cuts at: 1872 4447 1872
Size: 2575 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2898 3230 3877 5224 6125 6387
Size: 197 926 955 332 647 1347 901 262
Cuts at: 6387 820
Size: 1553
Fragments arranged by size:
1553 1347 955 926 901 647 332 262
197
SnaBI TAC'GTA
Cuts at: 493 4911 493
Size: 4418 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7120
SphI G_CATG'C
Cuts at: 1132 4478 1132
Size: 3346 3774
SspI AAT'ATT
Cuts at: 5 52 5044 5
Size: 47 4992 2081
Fragments arranged by size:
4992 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7120
StyI C'CwwG_G
Cuts at: 513 1065 2548 3426 4559 513
Size: 552 1483 878 1133 3074
Fragments arranged by size:
3074 1483 1133 878 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5147 5164 5317 5502 5947 325
Size: 1255 3567 17 153 185 445 1498
Fragments arranged by size:
3567 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3315 3369 5366
Size: 274 80 33 51 1443 1338 54 1997
Cuts at: 5366 7078 96
Size: 1712 138
Fragments arranged by size:
1997 1712 1443 1338 274 138 80 54
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2864 2896 7080 7112 2864
Size: 32 4184 32 2872
Fragments arranged by size:
4184 2872 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3789 1316
Size: 2473 4647
XbaI T'CTAG_A
Cuts at: 4456 4456
Size: 7120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2461 3202 3433 3607 2011
Size: 450 741 231 174 5524
Fragments arranged by size:
5524 741 450 231 174
XhoI C'TCGA_G
Cuts at: 1943 2898 3230 1943
Size: 955 332 5833
Fragments arranged by size:
5833 955 332
XmaI C'CCGG_G
Cuts at: 1870 4445 1870
Size: 2575 4545
XmnI GAAnn'nnTTC
Cuts at: 3668 5249 3668
Size: 1581 5539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4928 276
Size: 53 83 186 936 3394 2468
Fragments arranged by size:
3394 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI
RsrII SacII SfiI SfoI SrfI SwaI