Restriction map for
pFN21AE5710
(Circular) (Six-base) MAPSORT of: pFN21AE5710.seq Check: 3138 from: 1 to: 7240
pFN21AE5710 7240 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5050 278
Size: 53 83 186 936 3514 2468
Fragments arranged by size:
3514 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4583 1937
Size: 2646 4594
Acc65I G'GTAC_C
Cuts at: 4561 4561
Size: 7240
AclI AA'CG_TT
Cuts at: 1563 2123 5367 5740 1563
Size: 560 3244 373 3063
Fragments arranged by size:
3244 3063 560 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3179 3698 3707 3830 5303 6687 6955 1632
Size: 1547 519 9 123 1473 1384 268 1917
Fragments arranged by size:
1917 1547 1473 1384 519 268 123 9
AflII C'TTAA_G
Cuts at: 820 1017 2306 820
Size: 197 1289 5754
Fragments arranged by size:
5754 1289 197
AflIII A'CryG_T
Cuts at: 1203 6139 7116 1203
Size: 4936 977 1327
Fragments arranged by size:
4936 1327 977
AgeI A'CCGG_T
Cuts at: 6071 6071
Size: 7240
AhdI GACnn_n'nnGTC
Cuts at: 4330 5969 4330
Size: 1639 5601
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7240
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7240
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4275 4307 4275
Size: 32 7208
AlwNI CAG_nnn'CTG
Cuts at: 3230 4983 6555 3230
Size: 1753 1572 3915
Fragments arranged by size:
3915 1753 1572
ApaI G_GGCC'C
Cuts at: 3980 3980
Size: 7240
ApaLI G'TGCA_C
Cuts at: 5297 6453 5297
Size: 1156 6084
ApoI r'AATT_y
Cuts at: 1493 2579 4549 4775 4927 7036 1493
Size: 1086 1970 226 152 2109 1697
Fragments arranged by size:
2109 1970 1697 1086 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5794 160
Size: 872 4762 1606
Fragments arranged by size:
4762 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7240
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 4533 4565 1870
Size: 73 96 2494 32 4545
Fragments arranged by size:
4545 2494 96 73 32
BamHI G'GATC_C
Cuts at: 3303 4570 4937 3303
Size: 1267 367 5606
Fragments arranged by size:
5606 1267 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 4561 6016 618
Size: 325 217 627 227 2547 1455 1842
Fragments arranged by size:
2547 1842 1455 627 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2040 2072 2295 3064 3414 3438 3807 3980
Size: 649 32 223 769 350 24 369 173
Cuts at: 3980 4559 1391
Size: 579 4072
Fragments arranged by size:
4072 769 649 579 369 350 223 173
32 24
BbeI G_GCGC'C
Cuts at: 2018 2018
Size: 7240
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3506 4485 928
Size: 971 1607 979 3683
Fragments arranged by size:
3683 1607 979 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5431 5465 1903
Size: 34 50 34 3410 34 3678
Fragments arranged by size:
3678 3410 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5132 6342 5132
Size: 1210 6030
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7240
BfrBI ATG'CAT
Cuts at: 2906 2906
Size: 7240
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2672 3272 4191
Size: 107 122 71 631 605 1000 600 919
Cuts at: 4191 5851 136
Size: 1660 1525
Fragments arranged by size:
1660 1525 1000 919 631 605 600 122
107 71
BglII A'GATC_T
Cuts at: 4221 7235 4221
Size: 3014 4226
BlpI GC'TnA_GC
Cuts at: 2565 4266 4657 2565
Size: 1701 391 5148
Fragments arranged by size:
5148 1701 391
Bme1580I G_kGCm'C
Cuts at: 1790 3400 3980 5301 6457 1790
Size: 1610 580 1321 1156 2573
Fragments arranged by size:
2573 1610 1321 1156 580
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7240
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4971 5929
Size: 416 53 83 399 24 1862 1692 958
Cuts at: 5929 442
Size: 1753
Fragments arranged by size:
1862 1753 1692 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7240
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2896 3090 2896
Size: 194 7046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2942 3470 3855 4217 5900 1239
Size: 1703 528 385 362 1683 2579
Fragments arranged by size:
2579 1703 1683 528 385 362
Bpu10I CC'TnA_GC
Cuts at: 2457 3065 3801 2457
Size: 608 736 5896
Fragments arranged by size:
5896 736 608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 2507 3026 5365 6230 6528 2165
Size: 342 519 2339 865 298 2877
Fragments arranged by size:
2877 2339 865 519 342 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5903 882
Size: 617 4404 2219
Fragments arranged by size:
4404 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5031 493
Size: 711 3827 2702
Fragments arranged by size:
3827 2702 711
BsaBI GATnn'nnATC
Cuts at: 7234 7234
Size: 7240
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 4121 5047 5429
Size: 53 83 186 936 482 2106 926 382
Cuts at: 5429 6064 275
Size: 635 1451
Fragments arranged by size:
2106 1451 936 926 635 482 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5673 6071 6345 6492 6903 5673
Size: 398 274 147 411 6010
Fragments arranged by size:
6010 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 1178
Size: 30 165 30 624 30 6361
Fragments arranged by size:
6361 624 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2447 2450 2618 2729 2732 2742 3029
Size: 1165 96 3 168 111 3 10 287
Cuts at: 3029 3197 4130 4457 1186
Size: 168 933 327 3969
Fragments arranged by size:
3969 1165 933 327 287 168 168 111
96 10 3 3
BseYI C'CCAG_C
Cuts at: 2159 3573 3877 6443 6939 2159
Size: 1414 304 2566 496 2460
Fragments arranged by size:
2566 2460 1414 496 304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2502 3532 1163
Size: 573 766 1030 4871
Fragments arranged by size:
4871 1030 766 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2992 4690 5451 6479 1540
Size: 275 186 991 1698 761 1028 2301
Fragments arranged by size:
2301 1698 1028 991 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2066 2181 2295 3807 5301 5386 6457 2066
Size: 115 114 1512 1494 85 1071 2849
Fragments arranged by size:
2849 1512 1494 1071 115 114 85
BsmI GAATG_Cn'
Cuts at: 4751 4844 4751
Size: 93 7147
BsmBI CGTCTCn'nnnn_
Cuts at: 3520 4112 3520
Size: 592 6648
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2066 2072 2181 2295 3064 3400
Size: 399 250 26 6 109 114 769 336
Cuts at: 3400 3414 3438 3807 3980 4559 5301 5386 6457
Size: 14 24 369 173 579 742 85 1071
Cuts at: 6457 1391
Size: 2174
Fragments arranged by size:
2174 1071 769 742 579 399 369 336
250 173 114 109 85 26 24 14
6
BspEI T'CCGG_A
Cuts at: 6903 6903
Size: 7240
BspHI T'CATG_A
Cuts at: 5129 6859 5129
Size: 1730 5510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4143 4161 4595 844
Size: 3299 18 434 3489
Fragments arranged by size:
3489 3299 434 18
BsrBI CCG'CTC
Cuts at: 1394 1823 2942 3187 5127 1394
Size: 429 1119 245 1940 3507
Fragments arranged by size:
3507 1940 1119 429 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 3048 5735 5909 65
Size: 1183 1800 2687 174 1396
Fragments arranged by size:
2687 1800 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2987 4062 4527 5884 6071 1700
Size: 110 1177 1075 465 1357 187 2869
Fragments arranged by size:
2869 1357 1177 1075 465 187 110
BsrGI T'GTAC_A
Cuts at: 96 2976 96
Size: 2880 4360
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7240
BssSI C'ACGA_G
Cuts at: 2065 5300 6312 2065
Size: 3235 1012 2993
Fragments arranged by size:
3235 2993 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2837 794
Size: 2043 5197
BstBI TT'CG_AA
Cuts at: 6044 6044
Size: 7240
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7240
BstXI CCAn_nnnn'nTGG
Cuts at: 3448 4321 4466 3448
Size: 873 145 6222
Fragments arranged by size:
6222 873 145
BstYI r'GATC_y
Cuts at: 1972 3303 4221 4570 4937 5322 5339 6791 6877
Size: 1331 918 349 367 385 17 1452 86
Cuts at: 6877 6889 7235 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1331 918 385 367 349 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2502 3819 2502
Size: 1317 5923
BtgI C'CryG_G
Cuts at: 513 1065 2365 4039 513
Size: 552 1300 1674 3714
Fragments arranged by size:
3714 1674 1300 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6501
Fragments arranged by size:
6501 683 56
BtsI GCAGTG_nn'
Cuts at: 3416 4756 5550 5570 3416
Size: 1340 794 20 5086
Fragments arranged by size:
5086 1340 794 20
ClaI AT'CG_AT
Cuts at: 1554 4464 1554
Size: 2910 4330
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7205
DraI TTT'AAA
Cuts at: 4544 4544
Size: 7240
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6247 809
Size: 507 4931 1802
Fragments arranged by size:
4931 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2989 3390 4687
Size: 54 1425 50 275 18 1159 401 1297
Cuts at: 4687 5576 7205 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1297 1159 889 401 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2989 4687 1537
Size: 275 1177 1698 4090
Fragments arranged by size:
4090 1698 1177 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2163 2277 2424 2538 2595 2706 3174
Size: 774 36 114 147 114 57 111 468
Cuts at: 3174 3989 5170 1353
Size: 815 1181 3423
Fragments arranged by size:
3423 1181 815 774 468 147 114 114
111 57 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2269 2885 3316 3511 3731 5827
Size: 1064 138 680 616 431 195 220 2096
Cuts at: 5827 6201 6347 387
Size: 374 146 1280
Fragments arranged by size:
2096 1280 1064 680 616 431 374 220
195 146 138
EcoICRI GAG'CTC
Cuts at: 2293 3805 2293
Size: 1512 5728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2942 3179 3470 3698 3707 3830 3855
Size: 393 1310 237 291 228 9 123 25
Cuts at: 3855 4217 5303 5900 6687 6955 1239
Size: 362 1086 597 787 268 1524
Fragments arranged by size:
1524 1310 1086 787 597 393 362 291
268 237 228 123 25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2411 2411
Size: 7240
EcoO109I rG'GnC_Cy
Cuts at: 3011 3796 3977 4504 3011
Size: 785 181 527 5747
Fragments arranged by size:
5747 785 527 181
EcoRI G'AATT_C
Cuts at: 4549 4927 4549
Size: 378 6862
EcoRV GAT'ATC
Cuts at: 3556 3556
Size: 7240
FspI TGC'GCA
Cuts at: 4109 5746 4109
Size: 1637 5603
HaeII r_GCGC'y
Cuts at: 1678 2018 2812 3817 6387 1678
Size: 340 794 1005 2570 2531
Fragments arranged by size:
2570 2531 1005 794 340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2401 2433 3770 3802 4316
Size: 32 135 32 829 32 1337 32 514
Cuts at: 4316 4348 5881 5913 5955 5987 1373
Size: 32 1533 32 42 32 2626
Fragments arranged by size:
2626 1533 1337 829 514 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4584 4830 669
Size: 988 281 2646 246 3079
Fragments arranged by size:
3079 2646 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7240
HpaI GTT'AAC
Cuts at: 4830 4830
Size: 7240
KasI G'GCGC_C
Cuts at: 2014 2014
Size: 7240
KpnI G_GTAC'C
Cuts at: 4565 4565
Size: 7240
MfeI C'AATT_G
Cuts at: 4420 4839 4420
Size: 419 6821
MluI A'CGCG_T
Cuts at: 7116 7116
Size: 7240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4479 6354 6538 1305
Size: 3174 1875 184 2007
Fragments arranged by size:
3174 2007 1875 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3392 10
Size: 54 1425 343 1560 3858
Fragments arranged by size:
3858 1560 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3086 3567 3950 5198 5557 5716 518
Size: 648 1920 481 383 1248 359 159 2042
Fragments arranged by size:
2042 1920 1248 648 481 383 359 159
MspA1I CmG'CkG
Cuts at: 1606 2367 2843 3205 3227 3281 3577 4105 4141
Size: 761 476 362 22 54 296 528 36
Cuts at: 4141 4429 4656 5333 6481 6726 1606
Size: 288 227 677 1148 245 2120
Fragments arranged by size:
2120 1148 761 677 528 476 362 296
288 245 227 54 36 22
NaeI GCC'GGC
Cuts at: 2989 4529 2989
Size: 1540 5700
NarI GG'CG_CC
Cuts at: 2015 2015
Size: 7240
NcoI C'CATG_G
Cuts at: 513 1065 4039 513
Size: 552 2974 3714
Fragments arranged by size:
3714 2974 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7240
NgoMIV G'CCGG_C
Cuts at: 2987 4527 2987
Size: 1540 5700
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7240
NotI GC'GGCC_GC
Cuts at: 4687 4687
Size: 7240
NsiI A_TGCA'T
Cuts at: 2908 2908
Size: 7240
NspI r_CATG'y
Cuts at: 1132 1229 3379 4598 6143 1132
Size: 97 2150 1219 1545 2229
Fragments arranged by size:
2229 2150 1545 1219 97
PasI CC'CwG_GG
Cuts at: 1348 4507 1348
Size: 3159 4081
PciI A'CATG_T
Cuts at: 6139 6139
Size: 7240
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7240
PmeI GTTT'AAAC
Cuts at: 4544 4544
Size: 7240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5309 5341 6822 6854 5309
Size: 32 1481 32 5695
Fragments arranged by size:
5695 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3011 3011
Size: 7240
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7240
PsiI TTA'TAA
Cuts at: 2627 4810 2627
Size: 2183 5057
PspOMI G'GGCC_C
Cuts at: 3976 3976
Size: 7240
PstI C_TGCA'G
Cuts at: 830 3286 4140 4592 830
Size: 2456 854 452 3478
Fragments arranged by size:
3478 2456 854 452
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7240
PvuII CAG'CTG
Cuts at: 2843 3205 3227 3281 4105 4429 2843
Size: 362 22 54 824 324 5654
Fragments arranged by size:
5654 824 362 324 54 22
SacI G_AGCT'C
Cuts at: 2295 3807 2295
Size: 1512 5728
SacII CC_GC'GG
Cuts at: 2368 2368
Size: 7240
SalI G'TCGA_C
Cuts at: 1936 4582 1936
Size: 2646 4594
SapI GCTCTTCn'nnn_
Cuts at: 2127 2127
Size: 7240
SbfI CC_TGCA'GG
Cuts at: 4592 4592
Size: 7240
ScaI AGT'ACT
Cuts at: 5488 7200 5488
Size: 1712 5528
SfcI C'TryA_G
Cuts at: 826 1045 3282 3589 4136 4588 5723 6404 6595
Size: 219 2237 307 547 452 1135 681 191
Cuts at: 6595 826
Size: 1471
Fragments arranged by size:
2237 1471 1135 681 547 452 307 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4191 4191
Size: 7240
SfoI GGC'GCC
Cuts at: 2016 2016
Size: 7240
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7240
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7240
SmaI CCC'GGG
Cuts at: 1872 4567 1872
Size: 2695 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2180 2306 2522 3041 5344 6245
Size: 197 926 237 126 216 519 2303 901
Cuts at: 6245 6507 820
Size: 262 1553
Fragments arranged by size:
2303 1553 926 901 519 262 237 216
197 126
SnaBI TAC'GTA
Cuts at: 493 5031 493
Size: 4538 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7240
SphI G_CATG'C
Cuts at: 1132 3379 4598 1132
Size: 2247 1219 3774
Fragments arranged by size:
3774 2247 1219
SspI AAT'ATT
Cuts at: 5 52 5164 5
Size: 47 5112 2081
Fragments arranged by size:
5112 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7240
StyI C'CwwG_G
Cuts at: 513 1065 2382 4039 4679 513
Size: 552 1317 1657 640 3074
Fragments arranged by size:
3074 1657 1317 640 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4423 4499 5267 5284 5437 5622 6067
Size: 1255 2843 76 768 17 153 185 445
Cuts at: 6067 325
Size: 1498
Fragments arranged by size:
2843 1498 1255 768 445 185 153 76
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2976 3825 5486
Size: 274 80 33 51 1443 999 849 1661
Cuts at: 5486 7198 96
Size: 1712 138
Fragments arranged by size:
1712 1661 1443 999 849 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2027 2059 3117 3149 3288 3320 7200 7232 2027
Size: 32 1058 32 139 32 3880 32 2035
Fragments arranged by size:
3880 2035 1058 139 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7240
XbaI T'CTAG_A
Cuts at: 4576 4576
Size: 7240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3445 3445
Size: 7240
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7240
XmaI C'CCGG_G
Cuts at: 1870 4565 1870
Size: 2695 4545
XmnI GAAnn'nnTTC
Cuts at: 5369 5369
Size: 7240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5048 276
Size: 53 83 186 936 3514 2468
Fragments arranged by size:
3514 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BsiWI BstZ17I
DraIII FalI FseI FspAI NruI PacI PfoI PmlI
PspXI PsrI RsrII SanDI SexAI SrfI SwaI