Restriction map for
pFN21AE5708
(Circular) (Six-base) MAPSORT of: pFN21AE5708.seq Check: 7928 from: 1 to: 6916
pFN21AE5708 6916 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4726 278
Size: 53 83 186 936 3190 2468
Fragments arranged by size:
3190 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3003 3156 4111 4120 4144 4259 1937
Size: 1066 153 955 9 24 115 4594
Fragments arranged by size:
4594 1066 955 153 115 24 9
Acc65I G'GTAC_C
Cuts at: 4237 4237
Size: 6916
AclI AA'CG_TT
Cuts at: 1563 5043 5416 1563
Size: 3480 373 3063
Fragments arranged by size:
3480 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2247 2460 2973 3189 3289 3390 4979 6363
Size: 615 213 513 216 100 101 1589 1384
Cuts at: 6363 6631 1632
Size: 268 1917
Fragments arranged by size:
1917 1589 1384 615 513 268 216 213
101 100
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6719
AflIII A'CryG_T
Cuts at: 1203 2396 2860 3541 5815 6792 1203
Size: 1193 464 681 2274 977 1327
Fragments arranged by size:
2274 1327 1193 977 681 464
AgeI A'CCGG_T
Cuts at: 5747 5747
Size: 6916
AhdI GACnn_n'nnGTC
Cuts at: 2508 5645 2508
Size: 3137 3779
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6916
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6916
AlwNI CAG_nnn'CTG
Cuts at: 3658 4659 6231 3658
Size: 1001 1572 4343
Fragments arranged by size:
4343 1572 1001
ApaLI G'TGCA_C
Cuts at: 4973 6129 4973
Size: 1156 5760
ApoI r'AATT_y
Cuts at: 1493 2255 4225 4451 4603 6712 1493
Size: 762 1970 226 152 2109 1697
Fragments arranged by size:
2109 1970 1697 762 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2763 5470 160
Size: 872 1731 2707 1606
Fragments arranged by size:
2707 1731 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
AvaI C'yCGr_G
Cuts at: 1870 1943 4241 1870
Size: 73 2298 4545
Fragments arranged by size:
4545 2298 73
BamHI G'GATC_C
Cuts at: 4246 4613 4246
Size: 367 6549
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2112 4237 5692 618
Size: 325 217 627 325 2125 1455 1842
Fragments arranged by size:
2125 1842 1455 627 325 325 217
BanII G_rGCy'C
Cuts at: 1391 4235 1391
Size: 2844 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2961 4165 928
Size: 971 1062 1204 3679
Fragments arranged by size:
3679 1204 1062 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5107 5141 1903
Size: 34 50 34 3086 34 3678
Fragments arranged by size:
3678 3086 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3957 4808 6018 3957
Size: 851 1210 4855
Fragments arranged by size:
4855 1210 851
BclI T'GATC_A
Cuts at: 1548 3744 1548
Size: 2196 4720
BfrBI ATG'CAT
Cuts at: 3686 3686
Size: 6916
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4074 5527 136
Size: 107 122 71 631 605 2402 1453 1525
Fragments arranged by size:
2402 1525 1453 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2287 6911 2287
Size: 4624 2292
BlpI GC'TnA_GC
Cuts at: 4333 4333
Size: 6916
Bme1580I G_kGCm'C
Cuts at: 1790 4977 6133 1790
Size: 3187 1156 2573
Fragments arranged by size:
3187 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6916
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3244 4647 5605
Size: 416 53 83 399 24 1827 1403 958
Cuts at: 5605 442
Size: 1753
Fragments arranged by size:
1827 1753 1403 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5576 1239
Size: 4337 2579
Bpu10I CC'TnA_GC
Cuts at: 4135 4135
Size: 6916
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2526 5041 5906 6204 2526
Size: 2515 865 298 3238
Fragments arranged by size:
3238 2515 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2381 3123 3188 3606 5579 882
Size: 617 882 742 65 418 1973 2219
Fragments arranged by size:
2219 1973 882 742 617 418 65
BsaAI yAC'GTr
Cuts at: 493 1204 2399 4707 493
Size: 711 1195 2308 2702
Fragments arranged by size:
2702 2308 1195 711
BsaBI GATnn'nnATC
Cuts at: 6910 6910
Size: 6916
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4723 5105 5740 275
Size: 53 83 186 936 3190 382 635 1451
Fragments arranged by size:
3190 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5349 5747 6021 6168 6579 5349
Size: 398 274 147 411 5686
Fragments arranged by size:
5686 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6691
Fragments arranged by size:
6691 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2009 2072 2399 3387 3390 4054 1186
Size: 823 63 327 988 3 664 4048
Fragments arranged by size:
4048 988 823 664 327 63 3
BseYI C'CCAG_C
Cuts at: 6119 6615 6119
Size: 496 6420
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3696 1163
Size: 573 1960 4383
Fragments arranged by size:
4383 1960 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4102 4366 5127 6155 1540
Size: 275 186 2101 264 761 1028 2301
Fragments arranged by size:
2301 2101 1028 761 275 264 186
BsiHKAI G_wGCw'C
Cuts at: 4977 5062 6133 4977
Size: 85 1071 5760
Fragments arranged by size:
5760 1071 85
BsmI GAATG_Cn'
Cuts at: 3250 4427 4520 3250
Size: 1177 93 5646
Fragments arranged by size:
5646 1177 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4235 4977 5062 6133 1391
Size: 399 2445 742 85 1071 2174
Fragments arranged by size:
2445 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6579 6579
Size: 6916
BspHI T'CATG_A
Cuts at: 2929 4805 6535 2929
Size: 1876 1730 3310
Fragments arranged by size:
3310 1876 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4271 844
Size: 3427 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2634 4803 1394
Size: 429 811 2169 3507
Fragments arranged by size:
3507 2169 811 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3267 5411 5585 65
Size: 1183 2019 2144 174 1396
Fragments arranged by size:
2144 2019 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4068 4077 4101 5560 5747 1700
Size: 110 2258 9 24 1459 187 2869
Fragments arranged by size:
2869 2258 1459 187 110 24 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6916
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6916
BssSI C'ACGA_G
Cuts at: 4976 5988 4976
Size: 1012 5904
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3683 3692 794
Size: 2889 9 4018
Fragments arranged by size:
4018 2889 9
BstBI TT'CG_AA
Cuts at: 2773 5720 2773
Size: 2947 3969
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6916
BstXI CCAn_nnnn'nTGG
Cuts at: 3431 3713 3778 3431
Size: 282 65 6569
Fragments arranged by size:
6569 282 65
BstYI r'GATC_y
Cuts at: 1972 2287 2842 4246 4613 4998 5015 6467 6553
Size: 315 555 1404 367 385 17 1452 86
Cuts at: 6553 6565 6911 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1404 555 385 367 346 315
86 17 12
BstZ17I GTA'TAC
Cuts at: 3004 3004
Size: 6916
Bsu36I CC'TnA_GG
Cuts at: 3402 3402
Size: 6916
BtgI C'CryG_G
Cuts at: 513 1065 3352 513
Size: 552 2287 4077
Fragments arranged by size:
4077 2287 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3410 487
Size: 683 56 2184 3993
Fragments arranged by size:
3993 2184 683 56
BtsI GCAGTG_nn'
Cuts at: 3684 3858 4432 5226 5246 3684
Size: 174 574 794 20 5354
Fragments arranged by size:
5354 794 574 174 20
ClaI AT'CG_AT
Cuts at: 1554 4180 1554
Size: 2626 4290
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3069 3104 3324 3359 3723 3758 529
Size: 35 2505 35 220 35 364 35 3687
Fragments arranged by size:
3687 2505 364 220 35 35 35 35
DraI TTT'AAA
Cuts at: 3322 3538 4220 3322
Size: 216 682 6018
Fragments arranged by size:
6018 682 216
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4128 5923 809
Size: 507 2812 1795 1802
Fragments arranged by size:
2812 1802 1795 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2833 3099 3219
Size: 54 1425 50 275 18 1003 266 120
Cuts at: 3219 4099 4363 5252 6881 8
Size: 880 264 889 1629 43
Fragments arranged by size:
1629 1425 1003 889 880 275 266 264
120 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4099 4363 1537
Size: 275 2287 264 4090
Fragments arranged by size:
4090 2287 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 2436 3660 4846 1353
Size: 1083 1224 1186 3423
Fragments arranged by size:
3423 1224 1186 1083
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5503 5877 6023 387
Size: 1064 138 3914 374 146 1280
Fragments arranged by size:
3914 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2247 2460 2973 3189 3289 3390 4979
Size: 393 615 213 513 216 100 101 1589
Cuts at: 4979 5576 6363 6631 1239
Size: 597 787 268 1524
Fragments arranged by size:
1589 1524 787 615 597 513 393 268
216 213 101 100
EcoRI G'AATT_C
Cuts at: 2255 4225 4603 2255
Size: 1970 378 4568
Fragments arranged by size:
4568 1970 378
FspI TGC'GCA
Cuts at: 5422 5422
Size: 6916
HaeII r_GCGC'y
Cuts at: 1678 6063 1678
Size: 4385 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2243 2275 2494
Size: 32 135 32 548 32 91 32 219
Cuts at: 2494 2526 3398 3430 3729 3760 3761 3792 5557
Size: 32 872 32 299 31 1 31 1765
Cuts at: 5557 5589 5631 5663 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1765 872 548 299 219 135 91
42 32 32 32 32 32 32 32
32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3049 3376 3500 4121 4145 4260
Size: 988 281 1111 327 124 621 24 115
Cuts at: 4260 4506 669
Size: 246 3079
Fragments arranged by size:
3079 1111 988 621 327 281 246 124
115 24
HindIII A'AGCT_T
Cuts at: 748 2453 3724 748
Size: 1705 1271 3940
Fragments arranged by size:
3940 1705 1271
HpaI GTT'AAC
Cuts at: 4506 4506
Size: 6916
KpnI G_GTAC'C
Cuts at: 4241 4241
Size: 6916
MfeI C'AATT_G
Cuts at: 4515 4515
Size: 6916
MluI A'CGCG_T
Cuts at: 6792 6792
Size: 6916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2684 2776 3037 3664 3981 6030 6214 1305
Size: 1379 92 261 627 317 2049 184 2007
Fragments arranged by size:
2049 2007 1379 627 317 261 184 92
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3101 3221 10
Size: 54 1425 343 1269 120 3705
Fragments arranged by size:
3705 1425 1269 343 120 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2118 4874 5233 5392 518
Size: 648 952 2756 359 159 2042
Fragments arranged by size:
2756 2042 952 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3810 4332 5009 6157 6402 1606
Size: 2204 522 677 1148 245 2120
Fragments arranged by size:
2204 2120 1148 677 522 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6364
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6916
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6916
NotI GC'GGCC_GC
Cuts at: 4363 4363
Size: 6916
NsiI A_TGCA'T
Cuts at: 3688 3688
Size: 6916
NspI r_CATG'y
Cuts at: 1132 1229 2864 4274 5819 1132
Size: 97 1635 1410 1545 2229
Fragments arranged by size:
2229 1635 1545 1410 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6916
PciI A'CATG_T
Cuts at: 2860 5815 2860
Size: 2955 3961
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6916
PmeI GTTT'AAAC
Cuts at: 4220 4220
Size: 6916
PmlI CAC'GTG
Cuts at: 2399 2399
Size: 6916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4985 5017 6498 6530 4985
Size: 32 1481 32 5371
Fragments arranged by size:
5371 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6916
PsiI TTA'TAA
Cuts at: 4486 4486
Size: 6916
PstI C_TGCA'G
Cuts at: 830 2829 3697 4268 830
Size: 1999 868 571 3478
Fragments arranged by size:
3478 1999 868 571
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6916
PvuII CAG'CTG
Cuts at: 3810 3810
Size: 6916
SalI G'TCGA_C
Cuts at: 1936 4119 4143 4258 1936
Size: 2183 24 115 4594
Fragments arranged by size:
4594 2183 115 24
SbfI CC_TGCA'GG
Cuts at: 4268 4268
Size: 6916
ScaI AGT'ACT
Cuts at: 5164 6876 5164
Size: 1712 5204
SexAI A'CCwGG_T
Cuts at: 2803 3378 2803
Size: 575 6341
SfcI C'TryA_G
Cuts at: 826 1045 2825 3157 3693 4264 5399 6080 6271
Size: 219 1780 332 536 571 1135 681 191
Cuts at: 6271 826
Size: 1471
Fragments arranged by size:
1780 1471 1135 681 571 536 332 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4074 4074
Size: 6916
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6916
SmaI CCC'GGG
Cuts at: 1872 4243 1872
Size: 2371 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2541 5020 5921 6183 820
Size: 197 926 598 2479 901 262 1553
Fragments arranged by size:
2479 1553 926 901 598 262 197
SnaBI TAC'GTA
Cuts at: 493 4707 493
Size: 4214 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6916
SphI G_CATG'C
Cuts at: 1132 4274 1132
Size: 3142 3774
SspI AAT'ATT
Cuts at: 5 52 4004 4840 5
Size: 47 3952 836 2081
Fragments arranged by size:
3952 2081 836 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6916
StyI C'CwwG_G
Cuts at: 513 1065 2052 2181 4355 513
Size: 552 987 129 2174 3074
Fragments arranged by size:
3074 2174 987 552 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3884 4943 4960 5113 5298 5743 325
Size: 1255 2304 1059 17 153 185 445 1498
Fragments arranged by size:
2304 1498 1255 1059 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5162 6874 96
Size: 274 80 33 51 1443 3185 1712 138
Fragments arranged by size:
3185 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6876 6908 6876
Size: 32 6884
Tth111I GACn'n_nGTC
Cuts at: 1316 4116 1316
Size: 2800 4116
XbaI T'CTAG_A
Cuts at: 4252 4252
Size: 6916
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2828 3257 3601 2828
Size: 429 344 6143
Fragments arranged by size:
6143 429 344
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6916
XmaI C'CCGG_G
Cuts at: 1870 4241 1870
Size: 2371 4545
XmnI GAAnn'nnTTC
Cuts at: 5045 5045
Size: 6916
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4724 276
Size: 53 83 186 936 3190 2468
Fragments arranged by size:
3190 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfoI SrfI SwaI