Restriction map for
pFN21AE5707
(Circular) (Six-base) MAPSORT of: pFN21AE5707.seq Check: 709 from: 1 to: 6667
pFN21AE5707 6667 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4477 278
Size: 53 83 186 936 2941 2468
Fragments arranged by size:
2941 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3424 4010 1937
Size: 1487 586 4594
Fragments arranged by size:
4594 1487 586
Acc65I G'GTAC_C
Cuts at: 3988 3988
Size: 6667
AclI AA'CG_TT
Cuts at: 1563 2745 4794 5167 1563
Size: 1182 2049 373 3063
Fragments arranged by size:
3063 2049 1182 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2268 2513 2558 2837 3855 4730 6114 6382
Size: 636 245 45 279 1018 875 1384 268
Cuts at: 6382 1632
Size: 1917
Fragments arranged by size:
1917 1384 1018 875 636 279 268 245
45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6470
AflIII A'CryG_T
Cuts at: 1203 5566 6543 1203
Size: 4363 977 1327
Fragments arranged by size:
4363 1327 977
AgeI A'CCGG_T
Cuts at: 5498 5498
Size: 6667
AhdI GACnn_n'nnGTC
Cuts at: 5396 5396
Size: 6667
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6667
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3078 1184
Size: 1894 4773
AlwNI CAG_nnn'CTG
Cuts at: 4410 5982 4410
Size: 1572 5095
ApaLI G'TGCA_C
Cuts at: 4724 5880 4724
Size: 1156 5511
ApoI r'AATT_y
Cuts at: 1493 2304 2723 3332 3953 3976 4202 4354 6463
Size: 811 419 609 621 23 226 152 2109
Cuts at: 6463 1493
Size: 1697
Fragments arranged by size:
2109 1697 811 621 609 419 226 152
23
AseI AT'TA_AT
Cuts at: 160 1032 3246 5221 160
Size: 872 2214 1975 1606
Fragments arranged by size:
2214 1975 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
AvaI C'yCGr_G
Cuts at: 1870 1943 3408 3992 1870
Size: 73 1465 584 4545
Fragments arranged by size:
4545 1465 584 73
BamHI G'GATC_C
Cuts at: 3997 4364 3997
Size: 367 6300
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3827 3988 5443 618
Size: 325 217 627 2040 161 1455 1842
Fragments arranged by size:
2040 1842 1455 627 325 217 161
BanII G_rGCy'C
Cuts at: 1391 2064 3986 1391
Size: 673 1922 4072
Fragments arranged by size:
4072 1922 673
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3794 928
Size: 971 1895 3801
Fragments arranged by size:
3801 1895 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2288 2322 3162 3196 4858 4892 1903
Size: 34 351 34 840 34 1662 34 3678
Fragments arranged by size:
3678 1662 840 351 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4559 5769 4559
Size: 1210 5457
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6667
BfrBI ATG'CAT
Cuts at: 3065 3065
Size: 6667
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5278 136
Size: 107 122 71 631 605 337 3269 1525
Fragments arranged by size:
3269 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6662 6662
Size: 6667
BlpI GC'TnA_GC
Cuts at: 2058 4084 2058
Size: 2026 4641
Bme1580I G_kGCm'C
Cuts at: 1790 4728 5884 1790
Size: 2938 1156 2573
Fragments arranged by size:
2938 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6667
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2424 2743 2796
Size: 416 53 83 399 24 1007 319 53
Cuts at: 2796 4398 5356 442
Size: 1602 958 1753
Fragments arranged by size:
1753 1602 1007 958 416 399 319 83
53 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2717 3290 3381 5327 1239
Size: 1478 573 91 1946 2579
Fragments arranged by size:
2579 1946 1478 573 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4792 5657 5955 4792
Size: 865 298 5504
Fragments arranged by size:
5504 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2075 5330 882
Size: 617 576 3255 2219
Fragments arranged by size:
3255 2219 617 576
BsaAI yAC'GTr
Cuts at: 493 1204 4458 493
Size: 711 3254 2702
Fragments arranged by size:
3254 2702 711
BsaBI GATnn'nnATC
Cuts at: 6661 6661
Size: 6667
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 4474 4856 5491
Size: 53 83 186 936 509 2432 382 635
Cuts at: 5491 275
Size: 1451
Fragments arranged by size:
2432 1451 936 635 509 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2166 2599 5100 5498 5772 5919 6330 2166
Size: 433 2501 398 274 147 411 2503
Fragments arranged by size:
2503 2501 433 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2570 2600 1178
Size: 30 165 30 1167 30 5245
Fragments arranged by size:
5245 1167 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2020 2226 3879 1186
Size: 834 206 1653 3974
Fragments arranged by size:
3974 1653 834 206
BseYI C'CCAG_C
Cuts at: 2400 3894 5870 6366 2400
Size: 1494 1976 496 2701
Fragments arranged by size:
2701 1976 1494 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2773 3038 1163
Size: 573 1037 265 4792
Fragments arranged by size:
4792 1037 573 265
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2052 4117 4878 5906 1540
Size: 275 186 15 36 2065 761 1028 2301
Fragments arranged by size:
2301 2065 1028 761 275 186 36 15
BsiHKAI G_wGCw'C
Cuts at: 2064 4728 4813 5884 2064
Size: 2664 85 1071 2847
Fragments arranged by size:
2847 2664 1071 85
BsmI GAATG_Cn'
Cuts at: 4178 4271 4178
Size: 93 6574
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 3986 4728 4813 5884 1391
Size: 399 274 1922 742 85 1071 2174
Fragments arranged by size:
2174 1922 1071 742 399 274 85
BspEI T'CCGG_A
Cuts at: 2166 6330 2166
Size: 4164 2503
BspHI T'CATG_A
Cuts at: 4556 6286 4556
Size: 1730 4937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2395 4022 844
Size: 1551 1627 3489
Fragments arranged by size:
3489 1627 1551
BsrBI CCG'CTC
Cuts at: 1394 1823 4554 1394
Size: 429 2731 3507
Fragments arranged by size:
3507 2731 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5162 5336 65
Size: 1183 3914 174 1396
Fragments arranged by size:
3914 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5311 5498 1700
Size: 110 3501 187 2869
Fragments arranged by size:
3501 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6667
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6667
BssSI C'ACGA_G
Cuts at: 4727 5739 4727
Size: 1012 5655
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6667
BstBI TT'CG_AA
Cuts at: 5471 5471
Size: 6667
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6667
BstYI r'GATC_y
Cuts at: 1972 2159 2476 3997 4364 4749 4766 6218 6304
Size: 187 317 1521 367 385 17 1452 86
Cuts at: 6304 6316 6662 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1521 1452 385 367 346 317 187
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2032 2032
Size: 6667
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 513
Size: 552 939 48 5128
Fragments arranged by size:
5128 939 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5928
Fragments arranged by size:
5928 683 56
BtsI GCAGTG_nn'
Cuts at: 4183 4977 4997 4183
Size: 794 20 5853
Fragments arranged by size:
5853 794 20
ClaI AT'CG_AT
Cuts at: 1554 2875 1554
Size: 1321 5346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2746 2781 2817 2852 529
Size: 35 2182 35 36 35 4344
Fragments arranged by size:
4344 2182 36 35 35 35
DraI TTT'AAA
Cuts at: 3971 3971
Size: 6667
DraIII CAC_nnn'GTG
Cuts at: 2737 2737
Size: 6667
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5674 809
Size: 507 4358 1802
Fragments arranged by size:
4358 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2049 4114
Size: 54 1425 50 275 18 183 36 2065
Cuts at: 4114 5003 6632 8
Size: 889 1629 43
Fragments arranged by size:
2065 1629 1425 889 275 183 54 50
43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 2049 4114 1537
Size: 275 201 36 2065 4090
Fragments arranged by size:
4090 2065 275 201 36
EarI CTCTTCn'nnn_
Cuts at: 1353 2241 2577 3186 4597 1353
Size: 888 336 609 1411 3423
Fragments arranged by size:
3423 1411 888 609 336
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5254 5628 5774 387
Size: 1064 138 3665 374 146 1280
Fragments arranged by size:
3665 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2062 2062
Size: 6667
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2268 2513 2558 2717 2837 3290 3381
Size: 393 636 245 45 159 120 453 91
Cuts at: 3381 3855 4730 5327 6114 6382 1239
Size: 474 875 597 787 268 1524
Fragments arranged by size:
1524 875 787 636 597 474 453 393
268 245 159 120 91 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2208 2208
Size: 6667
EcoRI G'AATT_C
Cuts at: 3976 4354 3976
Size: 378 6289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3248 3248
Size: 6667
FspI TGC'GCA
Cuts at: 5173 5173
Size: 6667
HaeII r_GCGC'y
Cuts at: 1678 5814 1678
Size: 4136 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 3662 3694 5308
Size: 32 135 32 896 32 1162 32 1614
Cuts at: 5308 5340 5382 5414 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1614 1162 896 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4011 4257 669
Size: 988 281 2073 246 3079
Fragments arranged by size:
3079 2073 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2280 3119 3910 748
Size: 1532 839 791 3505
Fragments arranged by size:
3505 1532 839 791
HpaI GTT'AAC
Cuts at: 4257 4257
Size: 6667
KpnI G_GTAC'C
Cuts at: 3992 3992
Size: 6667
MfeI C'AATT_G
Cuts at: 2944 4266 2944
Size: 1322 5345
MluI A'CGCG_T
Cuts at: 6543 6543
Size: 6667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2559 5781 5965 1305
Size: 1254 3222 184 2007
Fragments arranged by size:
3222 2007 1254 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4845
Fragments arranged by size:
4845 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2850 3060 3810 4625 4984 5143 518
Size: 648 1684 210 750 815 359 159 2042
Fragments arranged by size:
2042 1684 815 750 648 359 210 159
MspA1I CmG'CkG
Cuts at: 1606 2296 3046 3898 4083 4760 5908 6153 1606
Size: 690 750 852 185 677 1148 245 2120
Fragments arranged by size:
2120 1148 852 750 690 677 245 185
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5176
Fragments arranged by size:
5176 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6667
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6667
NotI GC'GGCC_GC
Cuts at: 2013 4114 2013
Size: 2101 4566
NsiI A_TGCA'T
Cuts at: 3067 3067
Size: 6667
NspI r_CATG'y
Cuts at: 1132 1229 2549 3069 4025 5570 1132
Size: 97 1320 520 956 1545 2229
Fragments arranged by size:
2229 1545 1320 956 520 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6667
PciI A'CATG_T
Cuts at: 5566 5566
Size: 6667
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6667
PfoI T'CCnGG_A
Cuts at: 3659 3659
Size: 6667
PmeI GTTT'AAAC
Cuts at: 3971 3971
Size: 6667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4736 4768 6249 6281 4736
Size: 32 1481 32 5122
Fragments arranged by size:
5122 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3023 1613
Size: 1410 5257
PsiI TTA'TAA
Cuts at: 3950 4237 3950
Size: 287 6380
PstI C_TGCA'G
Cuts at: 830 2392 4019 830
Size: 1562 1627 3478
Fragments arranged by size:
3478 1627 1562
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6667
PvuII CAG'CTG
Cuts at: 2296 3898 2296
Size: 1602 5065
SacI G_AGCT'C
Cuts at: 2064 2064
Size: 6667
SalI G'TCGA_C
Cuts at: 1936 4009 1936
Size: 2073 4594
SapI GCTCTTCn'nnn_
Cuts at: 2577 2577
Size: 6667
SbfI CC_TGCA'GG
Cuts at: 4019 4019
Size: 6667
ScaI AGT'ACT
Cuts at: 4915 6627 4915
Size: 1712 4955
SfcI C'TryA_G
Cuts at: 826 1045 2388 2970 3425 4015 5150 5831 6022
Size: 219 1343 582 455 590 1135 681 191
Cuts at: 6022 826
Size: 1471
Fragments arranged by size:
1471 1343 1135 681 590 582 455 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6667
SmaI CCC'GGG
Cuts at: 1872 3994 1872
Size: 2122 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4771 5672 5934 820
Size: 197 926 2828 901 262 1553
Fragments arranged by size:
2828 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4458 493
Size: 3965 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6667
SphI G_CATG'C
Cuts at: 1132 4025 1132
Size: 2893 3774
SspI AAT'ATT
Cuts at: 5 52 3499 4591 5
Size: 47 3447 1092 2081
Fragments arranged by size:
3447 2081 1092 47
StuI AGG'CCT
Cuts at: 1517 2096 1517
Size: 579 6088
StyI C'CwwG_G
Cuts at: 513 1065 2004 4106 513
Size: 552 939 2102 3074
Fragments arranged by size:
3074 2102 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2725 3908 4694 4711 4864 5049 5494
Size: 1255 1145 1183 786 17 153 185 445
Cuts at: 5494 325
Size: 1498
Fragments arranged by size:
1498 1255 1183 1145 786 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4913 6625 96
Size: 274 80 33 51 1443 2936 1712 138
Fragments arranged by size:
2936 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6627 6659 6627
Size: 32 6635
Tth111I GACn'n_nGTC
Cuts at: 1316 2776 3011 1316
Size: 1460 235 4972
Fragments arranged by size:
4972 1460 235
XbaI T'CTAG_A
Cuts at: 2518 2780 4003 2518
Size: 262 1223 5182
Fragments arranged by size:
5182 1223 262
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6667
XmaI C'CCGG_G
Cuts at: 1870 3992 1870
Size: 2122 4545
XmnI GAAnn'nnTTC
Cuts at: 2526 2727 4796 2526
Size: 201 2069 4397
Fragments arranged by size:
4397 2069 201
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4475 276
Size: 53 83 186 936 2941 2468
Fragments arranged by size:
2941 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI