Restriction map for
pFN21AE5704
(Circular) (Six-base) MAPSORT of: pFN21AE5704.seq Check: 1512 from: 1 to: 6691
pFN21AE5704 6691 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4501 278
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2675 2767 4034 1937
Size: 738 92 1267 4594
Fragments arranged by size:
4594 1267 738 92
Acc65I G'GTAC_C
Cuts at: 4012 4012
Size: 6691
AclI AA'CG_TT
Cuts at: 1563 4818 5191 1563
Size: 3255 373 3063
Fragments arranged by size:
3255 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2567 2991 4754 6138 6406 1632
Size: 935 424 1763 1384 268 1917
Fragments arranged by size:
1917 1763 1384 935 424 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6494
AflIII A'CryG_T
Cuts at: 1203 5590 6567 1203
Size: 4387 977 1327
Fragments arranged by size:
4387 1327 977
AgeI A'CCGG_T
Cuts at: 5522 5522
Size: 6691
AhdI GACnn_n'nnGTC
Cuts at: 5420 5420
Size: 6691
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6691
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6691
AlwNI CAG_nnn'CTG
Cuts at: 3806 4434 6006 3806
Size: 628 1572 4491
Fragments arranged by size:
4491 1572 628
ApaLI G'TGCA_C
Cuts at: 4748 5904 4748
Size: 1156 5535
ApoI r'AATT_y
Cuts at: 1493 2618 2954 3212 3264 3826 3935 4000 4226
Size: 1125 336 258 52 562 109 65 226
Cuts at: 4226 4378 6487 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1125 562 336 258 226 152
109 65 52
AseI AT'TA_AT
Cuts at: 160 1032 5245 160
Size: 872 4213 1606
Fragments arranged by size:
4213 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
AvaI C'yCGr_G
Cuts at: 1870 1943 4016 1870
Size: 73 2073 4545
Fragments arranged by size:
4545 2073 73
BamHI G'GATC_C
Cuts at: 4021 4388 4021
Size: 367 6324
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4012 5467 618
Size: 325 217 627 2225 1455 1842
Fragments arranged by size:
2225 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2698 4010 1391
Size: 1307 1312 4072
Fragments arranged by size:
4072 1312 1307
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5720
BbvCI CC'TCA_GC
Cuts at: 3288 3288
Size: 6691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4882 4916 1903
Size: 34 2945 34 3678
Fragments arranged by size:
3678 2945 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3612 4583 5793 3612
Size: 971 1210 4510
Fragments arranged by size:
4510 1210 971
BclI T'GATC_A
Cuts at: 1548 2706 1548
Size: 1158 5533
BfrBI ATG'CAT
Cuts at: 3565 3565
Size: 6691
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5302 136
Size: 107 122 71 631 605 3630 1525
Fragments arranged by size:
3630 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6686 6686
Size: 6691
BlpI GC'TnA_GC
Cuts at: 4108 4108
Size: 6691
Bme1580I G_kGCm'C
Cuts at: 1790 2758 3241 4752 5908 1790
Size: 968 483 1511 1156 2573
Fragments arranged by size:
2573 1511 1156 968 483
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6691
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2768 3150 4422
Size: 416 53 83 399 24 1351 382 1272
Cuts at: 4422 5380 442
Size: 958 1753
Fragments arranged by size:
1753 1351 1272 958 416 399 382 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3656 1056
Size: 2600 4091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3302 5351 1239
Size: 2063 2049 2579
Fragments arranged by size:
2579 2063 2049
Bpu10I CC'TnA_GC
Cuts at: 3288 3756 3288
Size: 468 6223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3693 4816 5681 5979 3693
Size: 1123 865 298 4405
Fragments arranged by size:
4405 1123 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5354 882
Size: 617 3855 2219
Fragments arranged by size:
3855 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4482 493
Size: 711 3278 2702
Fragments arranged by size:
3278 2702 711
BsaBI GATnn'nnATC
Cuts at: 6685 6685
Size: 6691
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4498 4880 5515 275
Size: 53 83 186 936 2965 382 635 1451
Fragments arranged by size:
2965 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2908 5124 5522 5796 5943 6354 2908
Size: 2216 398 274 147 411 3245
Fragments arranged by size:
3245 2216 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2634 2664 3136 3166 3339
Size: 30 165 30 1231 30 472 30 173
Cuts at: 3339 3369 1178
Size: 30 4500
Fragments arranged by size:
4500 1231 472 173 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3276 3769 1186
Size: 2090 493 4108
Fragments arranged by size:
4108 2090 493
BseYI C'CCAG_C
Cuts at: 5894 6390 5894
Size: 496 6195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6118
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2142 2146 4141 4902 5930 1540
Size: 275 186 141 4 1995 761 1028 2301
Fragments arranged by size:
2301 1995 1028 761 275 186 141 4
BsiHKAI G_wGCw'C
Cuts at: 4752 4837 5908 4752
Size: 85 1071 5535
Fragments arranged by size:
5535 1071 85
BsmI GAATG_Cn'
Cuts at: 3565 4202 4295 3565
Size: 637 93 5961
Fragments arranged by size:
5961 637 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3675 3675
Size: 6691
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2698 2758 3241 4010 4752 4837 5908
Size: 399 908 60 483 769 742 85 1071
Cuts at: 5908 1391
Size: 2174
Fragments arranged by size:
2174 1071 908 769 742 483 399 85
60
BspEI T'CCGG_A
Cuts at: 2908 6354 2908
Size: 3446 3245
BspHI T'CATG_A
Cuts at: 4580 6310 4580
Size: 1730 4961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4046 844
Size: 3202 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2057 4578 1394
Size: 429 234 2521 3507
Fragments arranged by size:
3507 2521 429 234
BsrDI GCAATG_nn'
Cuts at: 65 1248 2875 3819 5186 5360 65
Size: 1183 1627 944 1367 174 1396
Fragments arranged by size:
1627 1396 1367 1183 944 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 5335 5522 1700
Size: 110 283 3242 187 2869
Fragments arranged by size:
3242 2869 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6691
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6691
BssSI C'ACGA_G
Cuts at: 2204 4751 5763 2204
Size: 2547 1012 3132
Fragments arranged by size:
3132 2547 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3541 794
Size: 2747 3944
BstBI TT'CG_AA
Cuts at: 5495 5495
Size: 6691
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6691
BstYI r'GATC_y
Cuts at: 1972 2542 2905 4021 4388 4773 4790 6242 6328
Size: 570 363 1116 367 385 17 1452 86
Cuts at: 6328 6340 6686 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1116 570 385 367 363 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2676 2676
Size: 6691
BtgI C'CryG_G
Cuts at: 513 1065 2118 513
Size: 552 1053 5086
Fragments arranged by size:
5086 1053 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5952
Fragments arranged by size:
5952 683 56
BtsI GCAGTG_nn'
Cuts at: 2611 4207 5001 5021 2611
Size: 1596 794 20 4281
Fragments arranged by size:
4281 1596 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6656
DraI TTT'AAA
Cuts at: 2953 3995 2953
Size: 1042 5649
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5698 809
Size: 507 4382 1802
Fragments arranged by size:
4382 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2175 4138
Size: 54 1425 50 275 18 313 32 1963
Cuts at: 4138 5027 6656 8
Size: 889 1629 43
Fragments arranged by size:
1963 1629 1425 889 313 275 54 50
43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2143 4138 1537
Size: 275 331 1995 4090
Fragments arranged by size:
4090 1995 331 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2160 2799 3018 3909 4621 1353
Size: 807 639 219 891 712 3423
Fragments arranged by size:
3423 891 807 712 639 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2067 5278 5652 5798 387
Size: 1064 138 478 3211 374 146 1280
Fragments arranged by size:
3211 1280 1064 478 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2567 2991 3302 4754 5351 6138 6406
Size: 393 935 424 311 1452 597 787 268
Cuts at: 6406 1239
Size: 1524
Fragments arranged by size:
1524 1452 935 787 597 424 393 311
268
EcoO109I rG'GnC_Cy
Cuts at: 3281 3281
Size: 6691
EcoRI G'AATT_C
Cuts at: 3212 4000 4378 3212
Size: 788 378 5525
Fragments arranged by size:
5525 788 378
EcoRV GAT'ATC
Cuts at: 2749 2749
Size: 6691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3982 3982
Size: 6691
FspI TGC'GCA
Cuts at: 5197 5197
Size: 6691
HaeII r_GCGC'y
Cuts at: 1678 5838 1678
Size: 4160 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2754 2786 3640 3672 3884
Size: 32 135 32 1182 32 854 32 212
Cuts at: 3884 3916 5332 5364 5406 5438 1373
Size: 32 1416 32 42 32 2626
Fragments arranged by size:
2626 1416 1182 854 212 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3909 4035 4281 669
Size: 988 281 1971 126 246 3079
Fragments arranged by size:
3079 1971 988 281 246 126
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6691
HpaI GTT'AAC
Cuts at: 3909 4281 3909
Size: 372 6319
KpnI G_GTAC'C
Cuts at: 4016 4016
Size: 6691
MfeI C'AATT_G
Cuts at: 3105 4290 3105
Size: 1185 5506
MluI A'CGCG_T
Cuts at: 6567 6567
Size: 6691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2013 3006 3742 5805 5989 1305
Size: 708 993 736 2063 184 2007
Fragments arranged by size:
2063 2007 993 736 708 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4869
Fragments arranged by size:
4869 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2761 4649 5008 5167 518
Size: 648 1595 1888 359 159 2042
Fragments arranged by size:
2042 1888 1595 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2046 3044 4107 4784 5932 6177 1606
Size: 440 998 1063 677 1148 245 2120
Fragments arranged by size:
2120 1148 1063 998 677 440 245
NaeI GCC'GGC
Cuts at: 2095 2095
Size: 6691
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6139
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6691
NgoMIV G'CCGG_C
Cuts at: 2093 2093
Size: 6691
NheI G'CTAG_C
Cuts at: 1052 3652 1052
Size: 2600 4091
NotI GC'GGCC_GC
Cuts at: 4138 4138
Size: 6691
NsiI A_TGCA'T
Cuts at: 3567 3567
Size: 6691
NspI r_CATG'y
Cuts at: 1132 1229 2263 3524 4049 5594 1132
Size: 97 1034 1261 525 1545 2229
Fragments arranged by size:
2229 1545 1261 1034 525 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6691
PciI A'CATG_T
Cuts at: 5590 5590
Size: 6691
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6691
PmeI GTTT'AAAC
Cuts at: 3995 3995
Size: 6691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4760 4792 6273 6305 4760
Size: 32 1481 32 5146
Fragments arranged by size:
5146 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3281 3281
Size: 6691
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6691
PsiI TTA'TAA
Cuts at: 4261 4261
Size: 6691
PstI C_TGCA'G
Cuts at: 830 4043 830
Size: 3213 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6691
PvuII CAG'CTG
Cuts at: 3044 3044
Size: 6691
SalI G'TCGA_C
Cuts at: 1936 4033 1936
Size: 2097 4594
SapI GCTCTTCn'nnn_
Cuts at: 3018 3018
Size: 6691
SbfI CC_TGCA'GG
Cuts at: 4043 4043
Size: 6691
ScaI AGT'ACT
Cuts at: 3315 4939 6651 3315
Size: 1624 1712 3355
Fragments arranged by size:
3355 1712 1624
SexAI A'CCwGG_T
Cuts at: 2151 2151
Size: 6691
SfcI C'TryA_G
Cuts at: 826 1045 2441 3326 3784 4039 5174 5855 6046
Size: 219 1396 885 458 255 1135 681 191
Cuts at: 6046 826
Size: 1471
Fragments arranged by size:
1471 1396 1135 885 681 458 255 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6691
SmaI CCC'GGG
Cuts at: 1872 4018 1872
Size: 2146 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3708 4795 5696 5958 820
Size: 197 926 1765 1087 901 262 1553
Fragments arranged by size:
1765 1553 1087 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4482 493
Size: 3989 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6691
SphI G_CATG'C
Cuts at: 1132 4049 1132
Size: 2917 3774
SspI AAT'ATT
Cuts at: 5 52 2326 2399 2560 4615 5
Size: 47 2274 73 161 2055 2081
Fragments arranged by size:
2274 2081 2055 161 73 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6691
StyI C'CwwG_G
Cuts at: 513 1065 2739 4130 513
Size: 552 1674 1391 3074
Fragments arranged by size:
3074 1674 1391 552
SwaI ATTT'AAAT
Cuts at: 2953 2953
Size: 6691
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4718 4735 4888 5073 5518 325
Size: 1255 3138 17 153 185 445 1498
Fragments arranged by size:
3138 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2587 3313 4937
Size: 274 80 33 51 1443 610 726 1624
Cuts at: 4937 6649 96
Size: 1712 138
Fragments arranged by size:
1712 1624 1443 726 610 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2403 2435 6651 6683 2403
Size: 32 4216 32 2411
Fragments arranged by size:
4216 2411 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6691
XbaI T'CTAG_A
Cuts at: 4027 4027
Size: 6691
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6691
XmaI C'CCGG_G
Cuts at: 1870 4016 1870
Size: 2146 4545
XmnI GAAnn'nnTTC
Cuts at: 2012 4820 2012
Size: 2808 3883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4499 276
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI FseI
FspAI KasI NarI NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI
SrfI XcmI