Restriction map for
pFN21AE5703
(Circular) (Six-base) MAPSORT of: pFN21AE5703.seq Check: 8184 from: 1 to: 6646
pFN21AE5703 6646 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3122 4456 278
Size: 53 83 186 936 1586 1334 2468
Fragments arranged by size:
2468 1586 1334 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2874 3989 1937
Size: 937 1115 4594
Fragments arranged by size:
4594 1115 937
Acc65I G'GTAC_C
Cuts at: 3516 3967 3516
Size: 451 6195
AclI AA'CG_TT
Cuts at: 1563 4773 5146 1563
Size: 3210 373 3063
Fragments arranged by size:
3210 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2914 3290 3353 3624 3767 3800 3845 4709
Size: 1282 376 63 271 143 33 45 864
Cuts at: 4709 6093 6361 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1282 864 376 271 268 143
63 45 33
AfeI AGC'GCT
Cuts at: 2049 3374 2049
Size: 1325 5321
AflII C'TTAA_G
Cuts at: 820 1017 2915 820
Size: 197 1898 4551
Fragments arranged by size:
4551 1898 197
AflIII A'CryG_T
Cuts at: 1203 5545 6522 1203
Size: 4342 977 1327
Fragments arranged by size:
4342 1327 977
AgeI A'CCGG_T
Cuts at: 5477 5477
Size: 6646
AhdI GACnn_n'nnGTC
Cuts at: 5375 5375
Size: 6646
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6646
AlwNI CAG_nnn'CTG
Cuts at: 2096 2293 2864 3241 3823 4389 5961 2096
Size: 197 571 377 582 566 1572 2781
Fragments arranged by size:
2781 1572 582 571 566 377 197
ApaI G_GGCC'C
Cuts at: 2240 3422 3431 2240
Size: 1182 9 5455
Fragments arranged by size:
5455 1182 9
ApaLI G'TGCA_C
Cuts at: 3392 4703 5859 3392
Size: 1311 1156 4179
Fragments arranged by size:
4179 1311 1156
ApoI r'AATT_y
Cuts at: 1493 2414 3955 4181 4333 6442 1493
Size: 921 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 921 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5200 160
Size: 872 4168 1606
Fragments arranged by size:
4168 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
AvaI C'yCGr_G
Cuts at: 1870 1943 2233 2309 2474 2502 2652 3656 3844
Size: 73 290 76 165 28 150 1004 188
Cuts at: 3844 3971 1870
Size: 127 4545
Fragments arranged by size:
4545 1004 290 188 165 150 127 76
73 28
BamHI G'GATC_C
Cuts at: 3492 3976 4343 3492
Size: 484 367 5795
Fragments arranged by size:
5795 484 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2317 2832 3516 3967 5422
Size: 325 217 627 530 515 684 451 1455
Cuts at: 5422 618
Size: 1842
Fragments arranged by size:
1842 1455 684 627 530 515 451 325
217
BanII G_rGCy'C
Cuts at: 1391 2240 3020 3291 3422 3431 3509 3965 1391
Size: 849 780 271 131 9 78 456 4072
Fragments arranged by size:
4072 849 780 456 271 131 78 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3574 928
Size: 971 1675 4000
Fragments arranged by size:
4000 1675 971
BbvCI CC'TCA_GC
Cuts at: 2421 2421
Size: 6646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4837 4871 1903
Size: 34 50 34 2816 34 3678
Fragments arranged by size:
3678 2816 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4538 5748 4538
Size: 1210 5436
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6646
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2711 3426 5257
Size: 107 122 71 631 605 1039 715 1831
Cuts at: 5257 136
Size: 1525
Fragments arranged by size:
1831 1525 1039 715 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3477 6641 3477
Size: 3164 3482
BlpI GC'TnA_GC
Cuts at: 2009 3508 4063 2009
Size: 1499 555 4592
Fragments arranged by size:
4592 1499 555
Bme1580I G_kGCm'C
Cuts at: 1790 2240 2320 2599 2835 3396 3422 3431 4707
Size: 450 80 279 236 561 26 9 1276
Cuts at: 4707 5863 1790
Size: 1156 2573
Fragments arranged by size:
2573 1276 1156 561 450 279 236 80
26 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6646
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2555 2702 3801
Size: 416 53 83 399 24 1138 147 1099
Cuts at: 3801 4377 5335 442
Size: 576 958 1753
Fragments arranged by size:
1753 1138 1099 958 576 416 399 147
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6646
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2066 2066
Size: 6646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2511 3077 3121 3725 5306 1239
Size: 1272 566 44 604 1581 2579
Fragments arranged by size:
2579 1581 1272 604 566 44
Bpu10I CC'TnA_GC
Cuts at: 2421 3290 3300 2421
Size: 869 10 5767
Fragments arranged by size:
5767 869 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4771 5636 5934 4771
Size: 865 298 5483
Fragments arranged by size:
5483 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2499 3539 3645 3929 5309 882
Size: 617 1000 1040 106 284 1380 2219
Fragments arranged by size:
2219 1380 1040 1000 617 284 106
BsaAI yAC'GTr
Cuts at: 493 1204 4437 493
Size: 711 3233 2702
Fragments arranged by size:
3233 2702 711
BsaBI GATnn'nnATC
Cuts at: 2390 6640 2390
Size: 4250 2396
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3119 3763 4453 4835
Size: 53 83 186 936 1586 644 690 382
Cuts at: 4835 5470 275
Size: 635 1451
Fragments arranged by size:
1586 1451 936 690 644 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5079 5477 5751 5898 6309 5079
Size: 398 274 147 411 5416
Fragments arranged by size:
5416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2203 2233 1178
Size: 30 165 30 800 30 5591
Fragments arranged by size:
5591 800 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2438 2462 3098 3176 1186
Size: 973 279 24 636 78 4656
Fragments arranged by size:
4656 973 636 279 78 24
BseYI C'CCAG_C
Cuts at: 2021 2207 2369 2701 3026 3184 3255 3646 5849
Size: 186 162 332 325 158 71 391 2203
Cuts at: 5849 6345 2021
Size: 496 2322
Fragments arranged by size:
2322 2203 496 391 332 325 186 162
158 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3164 1163
Size: 573 1428 4645
Fragments arranged by size:
4645 1428 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2142 2394 4096 4857 5885 1540
Size: 275 186 141 252 1702 761 1028 2301
Fragments arranged by size:
2301 1702 1028 761 275 252 186 141
BsiHKAI G_wGCw'C
Cuts at: 3020 3291 3396 4707 4792 5863 3020
Size: 271 105 1311 85 1071 3803
Fragments arranged by size:
3803 1311 1071 271 105 85
BsmI GAATG_Cn'
Cuts at: 2758 4157 4250 2758
Size: 1399 93 5154
Fragments arranged by size:
5154 1399 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2129 3110 2129
Size: 981 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2320 2599 2835 3020 3291 3396
Size: 399 450 80 279 236 185 271 105
Cuts at: 3396 3422 3431 3509 3965 4707 4792 5863 1391
Size: 26 9 78 456 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 456 450 399 279 271
236 185 105 85 80 78 26 9
BspEI T'CCGG_A
Cuts at: 6309 6309
Size: 6646
BspHI T'CATG_A
Cuts at: 4535 6265 4535
Size: 1730 4916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3099 3122 3258 4001 844
Size: 2255 23 136 743 3489
Fragments arranged by size:
3489 2255 743 136 23
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 2018 3860 4533 1394
Size: 429 190 5 1842 673 3507
Fragments arranged by size:
3507 1842 673 429 190 5
BsrDI GCAATG_nn'
Cuts at: 65 1248 2691 5141 5315 65
Size: 1183 1443 2450 174 1396
Fragments arranged by size:
2450 1443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3339 5290 5477 1700
Size: 110 1529 1951 187 2869
Fragments arranged by size:
2869 1951 1529 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6646
BssSI C'ACGA_G
Cuts at: 4706 5718 4706
Size: 1012 5634
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2293 794
Size: 1499 5147
BstBI TT'CG_AA
Cuts at: 5450 5450
Size: 6646
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6646
BstXI CCAn_nnnn'nTGG
Cuts at: 2642 2642
Size: 6646
BstYI r'GATC_y
Cuts at: 1972 3147 3477 3492 3708 3976 4343 4728 4745
Size: 1175 330 15 216 268 367 385 17
Cuts at: 4745 6197 6283 6295 6641 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1175 385 367 346 330 268
216 86 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 3423 3423
Size: 6646
BtgI C'CryG_G
Cuts at: 513 1065 2572 3011 3483 3609 3907 513
Size: 552 1507 439 472 126 298 3252
Fragments arranged by size:
3252 1507 552 472 439 298 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2787 487
Size: 683 56 1561 4346
Fragments arranged by size:
4346 1561 683 56
BtsI GCAGTG_nn'
Cuts at: 2300 2941 3055 3379 4162 4956 4976 2300
Size: 641 114 324 783 794 20 3970
Fragments arranged by size:
3970 794 783 641 324 114 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2914 2949 529
Size: 35 2350 35 4226
Fragments arranged by size:
4226 2350 35 35
DraI TTT'AAA
Cuts at: 3950 3950
Size: 6646
DraIII CAC_nnn'GTG
Cuts at: 2680 2680
Size: 6646
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3220 5653 809
Size: 507 1904 2433 1802
Fragments arranged by size:
2433 1904 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2511 2793
Size: 54 1425 50 275 18 531 150 282
Cuts at: 2793 2893 4093 4982 6611 8
Size: 100 1200 889 1629 43
Fragments arranged by size:
1629 1425 1200 889 531 282 275 150
100 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4093 1537
Size: 275 2281 4090
Fragments arranged by size:
4090 2281 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 2277 4576 1353
Size: 911 13 2299 3423
Fragments arranged by size:
3423 2299 911 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2055 2938 5233 5607 5753 387
Size: 1064 138 466 883 2295 374 146 1280
Fragments arranged by size:
2295 1280 1064 883 466 374 146 138
EcoICRI GAG'CTC
Cuts at: 3018 3289 3018
Size: 271 6375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2511 2914 3077 3121 3290 3353 3624
Size: 393 879 403 163 44 169 63 271
Cuts at: 3624 3725 3767 3800 3845 4709 5306 6093 6361
Size: 101 42 33 45 864 597 787 268
Cuts at: 6361 1239
Size: 1524
Fragments arranged by size:
1524 879 864 787 597 403 393 271
268 169 163 101 63 45 44 42
33
EcoNI CCTnn'n_nnAGG
Cuts at: 2633 2633
Size: 6646
EcoO109I rG'GnC_Cy
Cuts at: 2237 2567 3418 3419 3427 3592 2237
Size: 330 851 1 8 165 5291
Fragments arranged by size:
5291 851 330 165 8 1
EcoRI G'AATT_C
Cuts at: 3955 4333 3955
Size: 378 6268
FspI TGC'GCA
Cuts at: 2582 5152 2582
Size: 2570 4076
HaeII r_GCGC'y
Cuts at: 1678 2051 3376 5793 1678
Size: 373 1325 2417 2531
Fragments arranged by size:
2531 2417 1325 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2656 2688 5287 5319 5361
Size: 32 135 32 1084 32 2599 32 42
Cuts at: 5361 5393 1373
Size: 32 2626
Fragments arranged by size:
2626 2599 1084 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3990 4236 669
Size: 988 281 2052 246 3079
Fragments arranged by size:
3079 2052 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6646
HpaI GTT'AAC
Cuts at: 4236 4236
Size: 6646
KpnI G_GTAC'C
Cuts at: 3520 3971 3520
Size: 451 6195
MfeI C'AATT_G
Cuts at: 4245 4245
Size: 6646
MluI A'CGCG_T
Cuts at: 6522 6522
Size: 6646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5760 5944 1305
Size: 4455 184 2007
Fragments arranged by size:
4455 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 2513 2795 2895 10
Size: 54 1425 343 531 150 282 100 3761
Fragments arranged by size:
3761 1425 531 343 282 150 100 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4604 4963 5122 518
Size: 648 3438 359 159 2042
Fragments arranged by size:
3438 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2103 2176 2293 2696 3013 3184 3238 4062
Size: 497 73 117 403 317 171 54 824
Cuts at: 4062 4739 5887 6132 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 824 677 497 403 317 245
171 117 73 54
NaeI GCC'GGC
Cuts at: 3341 3341
Size: 6646
NcoI C'CATG_G
Cuts at: 513 1065 3483 3609 513
Size: 552 2418 126 3550
Fragments arranged by size:
3550 2418 552 126
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6646
NgoMIV G'CCGG_C
Cuts at: 3339 3339
Size: 6646
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6646
NotI GC'GGCC_GC
Cuts at: 4093 4093
Size: 6646
NspI r_CATG'y
Cuts at: 1132 1229 2914 3400 4004 5549 1132
Size: 97 1685 486 604 1545 2229
Fragments arranged by size:
2229 1685 1545 604 486 97
PasI CC'CwG_GG
Cuts at: 1348 3261 3409 1348
Size: 1913 148 4585
Fragments arranged by size:
4585 1913 148
PciI A'CATG_T
Cuts at: 5545 5545
Size: 6646
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3184 1108
Size: 2076 4570
PmeI GTTT'AAAC
Cuts at: 3950 3950
Size: 6646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 6228 6260 4715
Size: 32 1481 32 5101
Fragments arranged by size:
5101 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6646
PsiI TTA'TAA
Cuts at: 4216 4216
Size: 6646
PspOMI G'GGCC_C
Cuts at: 2236 3418 3427 2236
Size: 1182 9 5455
Fragments arranged by size:
5455 1182 9
PstI C_TGCA'G
Cuts at: 830 3998 830
Size: 3168 3478
PvuI CG_AT'CG
Cuts at: 2001 2142 2394 2001
Size: 141 252 6253
Fragments arranged by size:
6253 252 141
PvuII CAG'CTG
Cuts at: 2103 3184 2103
Size: 1081 5565
SacI G_AGCT'C
Cuts at: 3020 3291 3020
Size: 271 6375
SacII CC_GC'GG
Cuts at: 3014 3014
Size: 6646
SalI G'TCGA_C
Cuts at: 1936 3988 1936
Size: 2052 4594
SapI GCTCTTCn'nnn_
Cuts at: 2277 2277
Size: 6646
SbfI CC_TGCA'GG
Cuts at: 3998 3998
Size: 6646
ScaI AGT'ACT
Cuts at: 4894 6606 4894
Size: 1712 4934
SexAI A'CCwGG_T
Cuts at: 2676 2676
Size: 6646
SfcI C'TryA_G
Cuts at: 826 1045 3322 3994 5129 5810 6001 826
Size: 219 2277 672 1135 681 191 1471
Fragments arranged by size:
2277 1471 1135 681 672 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3426 3426
Size: 6646
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6646
SmaI CCC'GGG
Cuts at: 1872 2654 3973 1872
Size: 782 1319 4545
Fragments arranged by size:
4545 1319 782
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2915 4750 5651 5913 820
Size: 197 926 972 1835 901 262 1553
Fragments arranged by size:
1835 1553 972 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4437 493
Size: 3944 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6646
SphI G_CATG'C
Cuts at: 1132 4004 1132
Size: 2872 3774
SspI AAT'ATT
Cuts at: 5 52 4570 5
Size: 47 4518 2081
Fragments arranged by size:
4518 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6646
StyI C'CwwG_G
Cuts at: 513 1065 2439 3483 3609 4085 513
Size: 552 1374 1044 126 476 3074
Fragments arranged by size:
3074 1374 1044 552 476 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3401 4673 4690 4843 5028 5473 325
Size: 1255 1821 1272 17 153 185 445 1498
Fragments arranged by size:
1821 1498 1272 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3531 4892 6604
Size: 274 80 33 51 1443 1554 1361 1712
Cuts at: 6604 96
Size: 138
Fragments arranged by size:
1712 1554 1443 1361 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2203 2235 6606 6638 2203
Size: 32 4371 32 2211
Fragments arranged by size:
4371 2211 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6646
XbaI T'CTAG_A
Cuts at: 3982 3982
Size: 6646
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6646
XmaI C'CCGG_G
Cuts at: 1870 2652 3971 1870
Size: 782 1319 4545
Fragments arranged by size:
4545 1319 782
XmnI GAAnn'nnTTC
Cuts at: 4775 4775
Size: 6646
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3120 4454 276
Size: 53 83 186 936 1586 1334 2468
Fragments arranged by size:
2468 1586 1334 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SanDI SfoI SrfI SwaI XcmI