Restriction map for
pFN21AE5701
(Circular) (Six-base) MAPSORT of: pFN21AE5701.seq Check: 6213 from: 1 to: 6949
pFN21AE5701 6949 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4759 278
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2808 3930 4292 1937
Size: 871 1122 362 4594
Fragments arranged by size:
4594 1122 871 362
Acc65I G'GTAC_C
Cuts at: 4270 4270
Size: 6949
AclI AA'CG_TT
Cuts at: 1563 5076 5449 1563
Size: 3513 373 3063
Fragments arranged by size:
3513 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2385 2423 3689 3752 4196 5012 6396 6664
Size: 753 38 1266 63 444 816 1384 268
Cuts at: 6664 1632
Size: 1917
Fragments arranged by size:
1917 1384 1266 816 753 444 268 63
38
AfeI AGC'GCT
Cuts at: 2948 3360 2948
Size: 412 6537
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6752
AflIII A'CryG_T
Cuts at: 1203 5848 6825 1203
Size: 4645 977 1327
Fragments arranged by size:
4645 1327 977
AgeI A'CCGG_T
Cuts at: 5780 5780
Size: 6949
AhdI GACnn_n'nnGTC
Cuts at: 5678 5678
Size: 6949
AleI CACnn'nnGTG
Cuts at: 1166 3096 3917 1166
Size: 1930 821 4198
Fragments arranged by size:
4198 1930 821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6949
AlwNI CAG_nnn'CTG
Cuts at: 3631 4692 6264 3631
Size: 1061 1572 4316
Fragments arranged by size:
4316 1572 1061
ApaI G_GGCC'C
Cuts at: 2323 2323
Size: 6949
ApaLI G'TGCA_C
Cuts at: 5006 6162 5006
Size: 1156 5793
ApoI r'AATT_y
Cuts at: 1493 2261 3782 4258 4484 4636 6745 1493
Size: 768 1521 476 226 152 2109 1697
Fragments arranged by size:
2109 1697 1521 768 476 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5503 160
Size: 872 4471 1606
Fragments arranged by size:
4471 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
AvaI C'yCGr_G
Cuts at: 1870 1943 2721 3743 3810 3872 4274 1870
Size: 73 778 1022 67 62 402 4545
Fragments arranged by size:
4545 1022 778 402 73 67 62
AvrII C'CTAG_G
Cuts at: 3070 3070
Size: 6949
BamHI G'GATC_C
Cuts at: 2437 4279 4646 2437
Size: 1842 367 4740
Fragments arranged by size:
4740 1842 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2863 3062 4270 5725 618
Size: 325 217 627 1076 199 1208 1455 1842
Fragments arranged by size:
1842 1455 1208 1076 627 325 217 199
BanII G_rGCy'C
Cuts at: 1391 2323 4152 4268 1391
Size: 932 1829 116 4072
Fragments arranged by size:
4072 1829 932 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3503 3900 928
Size: 971 1604 397 3977
Fragments arranged by size:
3977 1604 971 397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5140 5174 1903
Size: 34 3203 34 3678
Fragments arranged by size:
3678 3203 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4841 6051 4841
Size: 1210 5739
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6949
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5560 136
Size: 107 122 71 631 605 3888 1525
Fragments arranged by size:
3888 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3672 6944 3672
Size: 3272 3677
BlpI GC'TnA_GC
Cuts at: 3842 4151 4366 3842
Size: 309 215 6425
Fragments arranged by size:
6425 309 215
Bme1580I G_kGCm'C
Cuts at: 1790 2205 2323 3011 5010 6166 1790
Size: 415 118 688 1999 1156 2573
Fragments arranged by size:
2573 1999 1156 688 415 118
BmgBI CAC'GTC
Cuts at: 1318 2157 1318
Size: 839 6110
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2047 4059 4680
Size: 416 53 83 399 24 630 2012 621
Cuts at: 4680 5638 442
Size: 958 1753
Fragments arranged by size:
2012 1753 958 630 621 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6949
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2965 3715 2863
Size: 102 750 6097
Fragments arranged by size:
6097 750 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2691 3322 3656 5609 1239
Size: 1452 631 334 1953 2579
Fragments arranged by size:
2579 1953 1452 631 334
Bpu10I CC'TnA_GC
Cuts at: 2915 4194 2915
Size: 1279 5670
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2121 2254 2522 5074 5939 6237 2121
Size: 133 268 2552 865 298 2833
Fragments arranged by size:
2833 2552 865 298 268 133
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2423 5612 882
Size: 617 924 3189 2219
Fragments arranged by size:
3189 2219 924 617
BsaAI yAC'GTr
Cuts at: 493 1204 4740 493
Size: 711 3536 2702
Fragments arranged by size:
3536 2702 711
BsaBI GATnn'nnATC
Cuts at: 6943 6943
Size: 6949
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4756 5138 5773 275
Size: 53 83 186 936 3223 382 635 1451
Fragments arranged by size:
3223 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3111 5382 5780 6054 6201 6612 3111
Size: 2271 398 274 147 411 3448
Fragments arranged by size:
3448 2271 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2523 2553 4082 4112 1178
Size: 30 165 30 1120 30 1529 30 4015
Fragments arranged by size:
4015 1529 1120 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3995 4016 1186
Size: 2809 21 4119
Fragments arranged by size:
4119 2809 21
BseYI C'CCAG_C
Cuts at: 2069 2076 3171 6152 6648 2069
Size: 7 1095 2981 496 2370
Fragments arranged by size:
2981 2370 1095 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2066 2501 2534 3173 1163
Size: 573 330 435 33 639 4939
Fragments arranged by size:
4939 639 573 435 330 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3717 4399 5160 6188 1540
Size: 275 186 1716 682 761 1028 2301
Fragments arranged by size:
2301 1716 1028 761 682 275 186
BsiHKAI G_wGCw'C
Cuts at: 3050 4152 5010 5095 6166 3050
Size: 1102 858 85 1071 3833
Fragments arranged by size:
3833 1102 1071 858 85
BsmI GAATG_Cn'
Cuts at: 4460 4553 4460
Size: 93 6856
BsmBI CGTCTCn'nnnn_
Cuts at: 3961 3961
Size: 6949
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2323 3011 3050 4152 4268 5010
Size: 399 415 118 688 39 1102 116 742
Cuts at: 5010 5095 6166 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1102 1071 742 688 415 399 118
116 85 39
BspEI T'CCGG_A
Cuts at: 6612 6612
Size: 6949
BspHI T'CATG_A
Cuts at: 4838 6568 4838
Size: 1730 5219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4304 844
Size: 3460 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4836 1394
Size: 429 3013 3507
Fragments arranged by size:
3507 3013 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3207 5444 5618 65
Size: 1183 1959 2237 174 1396
Fragments arranged by size:
2237 1959 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 5593 5780 1700
Size: 110 227 3556 187 2869
Fragments arranged by size:
3556 2869 227 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6949
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6949
BssSI C'ACGA_G
Cuts at: 3313 5009 6021 3313
Size: 1696 1012 4241
Fragments arranged by size:
4241 1696 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2479 794
Size: 1685 5264
BstBI TT'CG_AA
Cuts at: 5753 5753
Size: 6949
BstEII G'GTnAC_C
Cuts at: 1184 2909 1184
Size: 1725 5224
BstYI r'GATC_y
Cuts at: 1972 2437 3672 4279 4646 5031 5048 6500 6586
Size: 465 1235 607 367 385 17 1452 86
Cuts at: 6586 6598 6944 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1235 607 465 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2868 2868
Size: 6949
BtgI C'CryG_G
Cuts at: 513 1065 2004 2433 3054 3348 513
Size: 552 939 429 621 294 4114
Fragments arranged by size:
4114 939 621 552 429 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3628 3861 487
Size: 683 56 2402 233 3575
Fragments arranged by size:
3575 2402 683 233 56
BtsI GCAGTG_nn'
Cuts at: 2484 4465 5259 5279 2484
Size: 1981 794 20 4154
Fragments arranged by size:
4154 1981 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6914
DraI TTT'AAA
Cuts at: 4253 4253
Size: 6949
DraIII CAC_nnn'GTG
Cuts at: 2479 2479
Size: 6949
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3173 5956 809
Size: 507 1857 2783 1802
Fragments arranged by size:
2783 1857 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3002 3051 4044
Size: 54 1425 50 275 18 1172 49 993
Cuts at: 4044 4396 5285 6914 8
Size: 352 889 1629 43
Fragments arranged by size:
1629 1425 1172 993 889 352 275 54
50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4396 1537
Size: 275 2584 4090
Fragments arranged by size:
4090 2584 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2233 2351 3546 3608 3684 3972 3980 4190
Size: 880 118 1195 62 76 288 8 210
Cuts at: 4190 4879 1353
Size: 689 3423
Fragments arranged by size:
3423 1195 880 689 288 210 118 76
62 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5536 5910 6056 387
Size: 1064 138 3947 374 146 1280
Fragments arranged by size:
3947 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4150 4150
Size: 6949
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2385 2423 2691 3322 3656 3689 3752
Size: 393 753 38 268 631 334 33 63
Cuts at: 3752 4196 5012 5609 6396 6664 1239
Size: 444 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 753 631 597 444 393
334 268 268 63 38 33
EcoO109I rG'GnC_Cy
Cuts at: 2073 2211 2319 3722 2073
Size: 138 108 1403 5300
Fragments arranged by size:
5300 1403 138 108
EcoRI G'AATT_C
Cuts at: 3782 4258 4636 3782
Size: 476 378 6095
Fragments arranged by size:
6095 476 378
EcoRV GAT'ATC
Cuts at: 2230 3409 2230
Size: 1179 5770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 3428 4201 2272
Size: 1156 773 5020
Fragments arranged by size:
5020 1156 773
FspI TGC'GCA
Cuts at: 5455 5455
Size: 6949
HaeII r_GCGC'y
Cuts at: 1678 2950 3362 6096 1678
Size: 1272 412 2734 2531
Fragments arranged by size:
2734 2531 1272 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2422 2454 2523 2555 2570
Size: 32 135 32 850 32 69 32 15
Cuts at: 2570 2602 3699 3731 4082 4114 5590 5622 5664
Size: 32 1097 32 351 32 1476 32 42
Cuts at: 5664 5696 1373
Size: 32 2626
Fragments arranged by size:
2626 1476 1097 850 351 135 69 42
32 32 32 32 32 32 32 32
32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2542 3427 3532 4293 4539 669
Size: 988 281 604 885 105 761 246 3079
Fragments arranged by size:
3079 988 885 761 604 281 246 105
HindIII A'AGCT_T
Cuts at: 748 4240 748
Size: 3492 3457
HpaI GTT'AAC
Cuts at: 4539 4539
Size: 6949
KpnI G_GTAC'C
Cuts at: 4274 4274
Size: 6949
MfeI C'AATT_G
Cuts at: 4548 4548
Size: 6949
MluI A'CGCG_T
Cuts at: 6825 6825
Size: 6949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2857 3126 3697 6063 6247 1305
Size: 1552 269 571 2366 184 2007
Fragments arranged by size:
2366 2007 1552 571 269 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4046 10
Size: 54 1425 343 2214 2913
Fragments arranged by size:
2913 2214 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2850 3096 3129 3545 3662 3755 3917
Size: 648 1684 246 33 416 117 93 162
Cuts at: 3917 4907 5266 5425 518
Size: 990 359 159 2042
Fragments arranged by size:
2042 1684 990 648 416 359 246 162
159 117 93 33
MspA1I CmG'CkG
Cuts at: 1606 2547 4365 5042 6190 6435 1606
Size: 941 1818 677 1148 245 2120
Fragments arranged by size:
2120 1818 1148 941 677 245
NaeI GCC'GGC
Cuts at: 2039 2039
Size: 6949
NcoI C'CATG_G
Cuts at: 513 1065 2004 3054 3348 513
Size: 552 939 1050 294 4114
Fragments arranged by size:
4114 1050 939 552 294
NdeI CA'TA_TG
Cuts at: 387 3663 387
Size: 3276 3673
NgoMIV G'CCGG_C
Cuts at: 2037 2037
Size: 6949
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6949
NotI GC'GGCC_GC
Cuts at: 4396 4396
Size: 6949
NspI r_CATG'y
Cuts at: 1132 1229 4307 5852 1132
Size: 97 3078 1545 2229
Fragments arranged by size:
3078 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3703 1348
Size: 2355 4594
PciI A'CATG_T
Cuts at: 5848 5848
Size: 6949
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2658 1108
Size: 1550 5399
PmeI GTTT'AAAC
Cuts at: 4253 4253
Size: 6949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5018 5050 6531 6563 5018
Size: 32 1481 32 5404
Fragments arranged by size:
5404 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2073 2211 2073
Size: 138 6811
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6949
PsiI TTA'TAA
Cuts at: 4519 4519
Size: 6949
PspOMI G'GGCC_C
Cuts at: 2319 2319
Size: 6949
PstI C_TGCA'G
Cuts at: 830 2929 3840 4224 4301 830
Size: 2099 911 384 77 3478
Fragments arranged by size:
3478 2099 911 384 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6949
PvuII CAG'CTG
Cuts at: 2547 2547
Size: 6949
SacI G_AGCT'C
Cuts at: 4152 4152
Size: 6949
SalI G'TCGA_C
Cuts at: 1936 4291 1936
Size: 2355 4594
SanDI GG'GwC_CC
Cuts at: 2073 2073
Size: 6949
SapI GCTCTTCn'nnn_
Cuts at: 4190 4190
Size: 6949
SbfI CC_TGCA'GG
Cuts at: 4301 4301
Size: 6949
ScaI AGT'ACT
Cuts at: 5197 6909 5197
Size: 1712 5237
SfcI C'TryA_G
Cuts at: 826 1045 2925 3836 4220 4297 5432 6113 6304
Size: 219 1880 911 384 77 1135 681 191
Cuts at: 6304 826
Size: 1471
Fragments arranged by size:
1880 1471 1135 911 681 384 219 191
77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6949
SmaI CCC'GGG
Cuts at: 1872 2723 4276 1872
Size: 851 1553 4545
Fragments arranged by size:
4545 1553 851
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2136 2233 2501 3743 5053 5954
Size: 197 926 193 97 268 1242 1310 901
Cuts at: 5954 6216 820
Size: 262 1553
Fragments arranged by size:
1553 1310 1242 926 901 268 262 197
193 97
SnaBI TAC'GTA
Cuts at: 493 4740 493
Size: 4247 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6949
SphI G_CATG'C
Cuts at: 1132 4307 1132
Size: 3175 3774
SspI AAT'ATT
Cuts at: 5 52 4873 5
Size: 47 4821 2081
Fragments arranged by size:
4821 2081 47
StuI AGG'CCT
Cuts at: 1517 2362 2978 3152 1517
Size: 845 616 174 5314
Fragments arranged by size:
5314 845 616 174
StyI C'CwwG_G
Cuts at: 513 1065 2004 2574 3011 3054 3070 3348 3564
Size: 552 939 570 437 43 16 278 216
Cuts at: 3564 4388 513
Size: 824 3074
Fragments arranged by size:
3074 939 824 570 552 437 278 216
43 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3674 4976 4993 5146 5331 5776 325
Size: 1255 2094 1302 17 153 185 445 1498
Fragments arranged by size:
2094 1498 1302 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5195 6907 96
Size: 274 80 33 51 1443 3218 1712 138
Fragments arranged by size:
3218 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2195 2227 6909 6941 2195
Size: 32 4682 32 2203
Fragments arranged by size:
4682 2203 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6949
XbaI T'CTAG_A
Cuts at: 4285 4285
Size: 6949
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3346 4132 3346
Size: 786 6163
XhoI C'TCGA_G
Cuts at: 1943 3743 1943
Size: 1800 5149
XmaI C'CCGG_G
Cuts at: 1870 2721 4274 1870
Size: 851 1553 4545
Fragments arranged by size:
4545 1553 851
XmnI GAAnn'nnTTC
Cuts at: 2466 4184 5078 2466
Size: 1718 894 4337
Fragments arranged by size:
4337 1718 894
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4757 276
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI BfrBI BsiWI
BstXI BstZ17I EcoNI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI