Restriction map for
pFN21AE5694
(Circular) (Six-base) MAPSORT of: pFN21AE5694.seq Check: 3711 from: 1 to: 6325
pFN21AE5694 6325 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2302 2821 3668 1937
Size: 365 519 847 4594
Fragments arranged by size:
4594 847 519 365
Acc65I G'GTAC_C
Cuts at: 3646 3646
Size: 6325
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2463 2751 3140 4388 5772 6040 1632
Size: 831 288 389 1248 1384 268 1917
Fragments arranged by size:
1917 1384 1248 831 389 288 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6128
AflIII A'CryG_T
Cuts at: 1203 5224 6201 1203
Size: 4021 977 1327
Fragments arranged by size:
4021 1327 977
AgeI A'CCGG_T
Cuts at: 5156 5156
Size: 6325
AhdI GACnn_n'nnGTC
Cuts at: 5054 5054
Size: 6325
AleI CACnn'nnGTG
Cuts at: 1166 2462 3398 1166
Size: 1296 936 4093
Fragments arranged by size:
4093 1296 936
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6325
AlwNI CAG_nnn'CTG
Cuts at: 3025 3050 3200 4068 5640 3025
Size: 25 150 868 1572 3710
Fragments arranged by size:
3710 1572 868 150 25
ApaLI G'TGCA_C
Cuts at: 3149 4382 5538 3149
Size: 1233 1156 3936
Fragments arranged by size:
3936 1233 1156
ApoI r'AATT_y
Cuts at: 1493 3634 3860 4012 6121 1493
Size: 2141 226 152 2109 1697
Fragments arranged by size:
2141 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2243 2243
Size: 6325
AseI AT'TA_AT
Cuts at: 160 1032 4879 160
Size: 872 3847 1606
Fragments arranged by size:
3847 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 2173 2616 2640 3345 3378 3650 1870
Size: 73 230 443 24 705 33 272 4545
Fragments arranged by size:
4545 705 443 272 230 73 33 24
BamHI G'GATC_C
Cuts at: 3655 4022 3655
Size: 367 5958
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2350 3487 3494 3646
Size: 325 217 627 272 291 1137 7 152
Cuts at: 3646 5101 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1137 627 325 291 272 217
152 7
BanII G_rGCy'C
Cuts at: 1391 2409 2800 3344 3644 1391
Size: 1018 391 544 300 4072
Fragments arranged by size:
4072 1018 544 391 300
BbeI G_GCGC'C
Cuts at: 2063 2354 2063
Size: 291 6034
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2961 3569 928
Size: 971 1062 608 3684
Fragments arranged by size:
3684 1062 971 608
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2164 2198 4516 4550 1903
Size: 34 227 34 2318 34 3678
Fragments arranged by size:
3678 2318 227 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4217 5427 4217
Size: 1210 5115
BclI T'GATC_A
Cuts at: 1548 2826 1548
Size: 1278 5047
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2026 4936 136
Size: 107 122 71 631 605 354 2910 1525
Fragments arranged by size:
2910 1525 631 605 354 122 107 71
BglII A'GATC_T
Cuts at: 6320 6320
Size: 6325
BlpI GC'TnA_GC
Cuts at: 3742 3742
Size: 6325
Bme1580I G_kGCm'C
Cuts at: 1790 3153 4386 5542 1790
Size: 1363 1233 1156 2573
Fragments arranged by size:
2573 1363 1233 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6325
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2114 2264 4056
Size: 416 53 83 399 24 697 150 1792
Cuts at: 4056 5014 442
Size: 958 1753
Fragments arranged by size:
1792 1753 958 697 416 399 150 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2282 1056
Size: 1226 5099
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 6325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 2735 2775 2855 3208 4985 1239
Size: 1184 312 40 80 353 1777 2579
Fragments arranged by size:
2579 1777 1184 353 312 80 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2271 3143 4450 5315 5613 2271
Size: 872 1307 865 298 2983
Fragments arranged by size:
2983 1307 872 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4988 882
Size: 617 3489 2219
Fragments arranged by size:
3489 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4116 493
Size: 711 2912 2702
Fragments arranged by size:
2912 2702 711
BsaBI GATnn'nnATC
Cuts at: 6319 6319
Size: 6325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 2351 4132 4514
Size: 53 83 186 936 527 291 1781 382
Cuts at: 4514 5149 275
Size: 635 1451
Fragments arranged by size:
1781 1451 936 635 527 382 291 186
83 53
BsaWI w'CCGG_w
Cuts at: 4758 5156 5430 5577 5988 4758
Size: 398 274 147 411 5095
Fragments arranged by size:
5095 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2309 2339 1178
Size: 30 165 30 906 30 5164
Fragments arranged by size:
5164 906 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6325
BseYI C'CCAG_C
Cuts at: 2941 3241 5528 6024 2941
Size: 300 2287 496 3242
Fragments arranged by size:
3242 2287 496 300
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 2486 3346 1163
Size: 573 666 84 860 4142
Fragments arranged by size:
4142 860 666 573 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2021 2225 2500 3775 4536 5564
Size: 275 186 20 204 275 1275 761 1028
Cuts at: 5564 1540
Size: 2301
Fragments arranged by size:
2301 1275 1028 761 275 275 204 186
20
BsiHKAI G_wGCw'C
Cuts at: 3153 3344 4386 4471 5542 3153
Size: 191 1042 85 1071 3936
Fragments arranged by size:
3936 1071 1042 191 85
BsmI GAATG_Cn'
Cuts at: 3836 3929 3836
Size: 93 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2409 2800 3153 3344 3644 4386 4471
Size: 399 619 391 353 191 300 742 85
Cuts at: 4471 5542 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 619 399 391 353 300
191 85
BspEI T'CCGG_A
Cuts at: 5988 5988
Size: 6325
BspHI T'CATG_A
Cuts at: 4214 5944 4214
Size: 1730 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3680 844
Size: 2836 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2688 4212 1394
Size: 429 865 1524 3507
Fragments arranged by size:
3507 1524 865 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4820 4994 65
Size: 1183 3572 174 1396
Fragments arranged by size:
3572 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 2104 2179 2239 2525 4969 5156
Size: 110 210 84 75 60 286 2444 187
Cuts at: 5156 1700
Size: 2869
Fragments arranged by size:
2869 2444 286 210 187 110 84 75
60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6325
BssHII G'CGCG_C
Cuts at: 1925 2243 2269 2271 2307 2335 2337 1925
Size: 318 26 2 36 28 2 5913
Fragments arranged by size:
5913 318 36 28 26 2 2
BssSI C'ACGA_G
Cuts at: 2039 4385 5397 2039
Size: 2346 1012 2967
Fragments arranged by size:
2967 2346 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6325
BstBI TT'CG_AA
Cuts at: 5129 5129
Size: 6325
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6325
BstYI r'GATC_y
Cuts at: 1972 3655 4022 4407 4424 5876 5962 5974 6320
Size: 1683 367 385 17 1452 86 12 346
Cuts at: 6320 1972
Size: 1977
Fragments arranged by size:
1977 1683 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3193 3278 3193
Size: 85 6240
BtgI C'CryG_G
Cuts at: 513 1065 2004 2725 513
Size: 552 939 721 4113
Fragments arranged by size:
4113 939 721 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5586
Fragments arranged by size:
5586 683 56
BtsI GCAGTG_nn'
Cuts at: 2536 2873 3841 4635 4655 2536
Size: 337 968 794 20 4206
Fragments arranged by size:
4206 968 794 337 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6290
DraI TTT'AAA
Cuts at: 3629 3629
Size: 6325
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2310 2829 5332 809
Size: 507 994 519 2503 1802
Fragments arranged by size:
2503 1802 994 519 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2018 2222 2497
Size: 54 1425 50 275 18 188 204 275
Cuts at: 2497 2784 3138 3772 4661 6290 8
Size: 287 354 634 889 1629 43
Fragments arranged by size:
1629 1425 889 634 354 287 275 275
204 188 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2018 2222 2497 3772 1537
Size: 275 206 204 275 1275 4090
Fragments arranged by size:
4090 1275 275 275 206 204
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 2915 3332 4255 1353
Size: 938 624 417 923 3423
Fragments arranged by size:
3423 938 923 624 417
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2179 4912 5286 5432 387
Size: 1064 138 590 2733 374 146 1280
Fragments arranged by size:
2733 1280 1064 590 374 146 138
EcoICRI GAG'CTC
Cuts at: 3342 3342
Size: 6325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 2463 2735 2751 2775 2855 3140
Size: 393 791 40 272 16 24 80 285
Cuts at: 3140 3208 4388 4985 5772 6040 1239
Size: 68 1180 597 787 268 1524
Fragments arranged by size:
1524 1180 791 787 597 393 285 272
268 80 68 40 24 16
EcoNI CCTnn'n_nnAGG
Cuts at: 3307 3307
Size: 6325
EcoO109I rG'GnC_Cy
Cuts at: 2071 2710 2809 3382 2071
Size: 639 99 573 5014
Fragments arranged by size:
5014 639 573 99
EcoRI G'AATT_C
Cuts at: 3634 4012 3634
Size: 378 5947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 6325
FspI TGC'GCA
Cuts at: 2114 4831 2114
Size: 2717 3608
FspAI rTGC'GCAy
Cuts at: 2114 2114
Size: 6325
HaeII r_GCGC'y
Cuts at: 1678 2063 2354 5472 1678
Size: 385 291 3118 2531
Fragments arranged by size:
3118 2531 385 291
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 2309 2341 2610
Size: 32 135 32 486 32 219 32 269
Cuts at: 2610 2642 3359 3391 4966 4998 5040 5072 1373
Size: 32 717 32 1575 32 42 32 2626
Fragments arranged by size:
2626 1575 717 486 269 219 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2303 2822 3669 3915 669
Size: 988 281 365 519 847 246 3079
Fragments arranged by size:
3079 988 847 519 365 281 246
HindIII A'AGCT_T
Cuts at: 748 2887 748
Size: 2139 4186
HpaI GTT'AAC
Cuts at: 3915 3915
Size: 6325
KasI G'GCGC_C
Cuts at: 2059 2350 2059
Size: 291 6034
KpnI G_GTAC'C
Cuts at: 3650 3650
Size: 6325
MfeI C'AATT_G
Cuts at: 3924 3924
Size: 6325
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 2838 3295 5439 5623 1305
Size: 816 717 457 2144 184 2007
Fragments arranged by size:
2144 2007 816 717 457 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2786 10
Size: 54 1425 343 954 3549
Fragments arranged by size:
3549 1425 954 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2462 3398 4283 4642 4801 518
Size: 648 954 342 936 885 359 159 2042
Fragments arranged by size:
2042 954 936 885 648 359 342 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2149 2203 2357 2560 2752 2945 3741
Size: 528 15 54 154 203 192 193 796
Cuts at: 3741 4418 5566 5811 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 796 677 528 245 203 193
192 154 54 15
NaeI GCC'GGC
Cuts at: 2241 2241
Size: 6325
NarI GG'CG_CC
Cuts at: 2060 2351 2060
Size: 291 6034
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4834
Fragments arranged by size:
4834 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6325
NgoMIV G'CCGG_C
Cuts at: 2239 2239
Size: 6325
NheI G'CTAG_C
Cuts at: 1052 2278 1052
Size: 1226 5099
NotI GC'GGCC_GC
Cuts at: 2497 3772 2497
Size: 1275 5050
NspI r_CATG'y
Cuts at: 1132 1229 2734 3683 5228 1132
Size: 97 1505 949 1545 2229
Fragments arranged by size:
2229 1545 1505 949 97
PasI CC'CwG_GG
Cuts at: 1348 3522 1348
Size: 2174 4151
PciI A'CATG_T
Cuts at: 5224 5224
Size: 6325
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2680 1108
Size: 1572 4753
PfoI T'CCnGG_A
Cuts at: 2892 3580 2892
Size: 688 5637
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4394 4426 5907 5939 4394
Size: 32 1481 32 4780
Fragments arranged by size:
4780 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2710 2710
Size: 6325
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6325
PsiI TTA'TAA
Cuts at: 3895 3895
Size: 6325
PstI C_TGCA'G
Cuts at: 830 2751 3677 830
Size: 1921 926 3478
Fragments arranged by size:
3478 1921 926
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
PvuII CAG'CTG
Cuts at: 2752 2945 2752
Size: 193 6132
RsrII CG'GwC_CG
Cuts at: 2080 2198 2080
Size: 118 6207
SacI G_AGCT'C
Cuts at: 3344 3344
Size: 6325
SalI G'TCGA_C
Cuts at: 1936 2301 2820 3667 1936
Size: 365 519 847 4594
Fragments arranged by size:
4594 847 519 365
SapI GCTCTTCn'nnn_
Cuts at: 2915 3332 2915
Size: 417 5908
SbfI CC_TGCA'GG
Cuts at: 3677 3677
Size: 6325
ScaI AGT'ACT
Cuts at: 4573 6285 4573
Size: 1712 4613
SfcI C'TryA_G
Cuts at: 826 1045 2129 2426 2747 3673 4808 5489 5680
Size: 219 1084 297 321 926 1135 681 191
Cuts at: 5680 826
Size: 1471
Fragments arranged by size:
1471 1135 1084 926 681 321 297 219
191
SfoI GGC'GCC
Cuts at: 2061 2352 2061
Size: 291 6034
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6325
SmaI CCC'GGG
Cuts at: 1872 3652 1872
Size: 1780 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2286 3158 4429 5330 5592 820
Size: 197 926 343 872 1271 901 262 1553
Fragments arranged by size:
1553 1271 926 901 872 343 262 197
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6325
SphI G_CATG'C
Cuts at: 1132 2734 3683 1132
Size: 1602 949 3774
Fragments arranged by size:
3774 1602 949
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 2622 1517
Size: 1105 5220
StyI C'CwwG_G
Cuts at: 513 1065 2004 2679 2691 3764 513
Size: 552 939 675 12 1073 3074
Fragments arranged by size:
3074 1073 939 675 552 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4352 4369 4522 4707 5152 325
Size: 1255 2772 17 153 185 445 1498
Fragments arranged by size:
2772 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4571 6283 96
Size: 274 80 33 51 1443 2594 1712 138
Fragments arranged by size:
2594 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6285 6317 6285
Size: 32 6293
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6325
XbaI T'CTAG_A
Cuts at: 3661 3661
Size: 6325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2799 2799
Size: 6325
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6325
XmaI C'CCGG_G
Cuts at: 1870 3650 1870
Size: 1780 4545
XmnI GAAnn'nnTTC
Cuts at: 2448 4454 2448
Size: 2006 4319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI
NruI NsiI PacI PmlI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SrfI SwaI