Restriction map for
pFN21AE5684
(Circular) (Six-base) MAPSORT of: pFN21AE5684.seq Check: 7643 from: 1 to: 6430
pFN21AE5684 6430 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3419 4240 278
Size: 53 83 186 936 1883 821 2468
Fragments arranged by size:
2468 1883 936 821 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3773 1937
Size: 1836 4594
Acc65I G'GTAC_C
Cuts at: 2676 3751 2676
Size: 1075 5355
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 2409 2577 4493 5877 6145 1632
Size: 527 250 168 1916 1384 268 1917
Fragments arranged by size:
1917 1916 1384 527 268 250 168
AfeI AGC'GCT
Cuts at: 3305 3305
Size: 6430
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 5329 6306 1203
Size: 4126 977 1327
Fragments arranged by size:
4126 1327 977
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6430
AlwNI CAG_nnn'CTG
Cuts at: 2182 4173 5745 2182
Size: 1991 1572 2867
Fragments arranged by size:
2867 1991 1572
ApaI G_GGCC'C
Cuts at: 2067 2225 3316 2067
Size: 158 1091 5181
Fragments arranged by size:
5181 1091 158
ApaLI G'TGCA_C
Cuts at: 3110 3188 4487 5643 3110
Size: 78 1299 1156 3897
Fragments arranged by size:
3897 1299 1156 78
ApoI r'AATT_y
Cuts at: 1493 3739 3965 4117 6226 1493
Size: 2246 226 152 2109 1697
Fragments arranged by size:
2246 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2585 2742 3115 3194 3307 3755 1870
Size: 73 642 157 373 79 113 448 4545
Fragments arranged by size:
4545 642 448 373 157 113 79 73
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2539 2573 2620 2676 2953
Size: 325 217 627 752 34 47 56 277
Cuts at: 2953 3125 3751 5206 618
Size: 172 626 1455 1842
Fragments arranged by size:
1842 1455 752 627 626 325 277 217
172 56 47 34
BanII G_rGCy'C
Cuts at: 1391 2067 2225 2242 2310 2507 2585 3122 3251
Size: 676 158 17 68 197 78 537 129
Cuts at: 3251 3316 3749 1391
Size: 65 433 4072
Fragments arranged by size:
4072 676 537 433 197 158 129 78
68 65 17
BbeI G_GCGC'C
Cuts at: 3129 3129
Size: 6430
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2649 928
Size: 971 750 4709
Fragments arranged by size:
4709 971 750
BbvCI CC'TCA_GC
Cuts at: 3317 3317
Size: 6430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3550 3584 4621 4655 1903
Size: 34 1613 34 1037 34 3678
Fragments arranged by size:
3678 1613 1037 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2747 3105 5041
Size: 107 122 71 631 605 1075 358 1936
Cuts at: 5041 136
Size: 1525
Fragments arranged by size:
1936 1525 1075 631 605 358 122 107
71
BglII A'GATC_T
Cuts at: 6425 6425
Size: 6430
BlpI GC'TnA_GC
Cuts at: 2484 3300 3847 2484
Size: 816 547 5067
Fragments arranged by size:
5067 816 547
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2225 2578 3114 3192 3316 4491 5647
Size: 277 158 353 536 78 124 1175 1156
Cuts at: 5647 1790
Size: 2573
Fragments arranged by size:
2573 1175 1156 536 353 277 158 124
78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6430
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4161 5119 442
Size: 416 53 83 399 24 2744 958 1753
Fragments arranged by size:
2744 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2028 2552 5090 1239
Size: 789 524 2538 2579
Fragments arranged by size:
2579 2538 789 524
Bpu10I CC'TnA_GC
Cuts at: 3317 3317
Size: 6430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2491 4555 5420 5718 2491
Size: 2064 865 298 3203
Fragments arranged by size:
3203 2064 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2582 2774 5093 882
Size: 617 1083 192 2319 2219
Fragments arranged by size:
2319 2219 1083 617 192
BsaAI yAC'GTr
Cuts at: 493 1204 2998 4221 493
Size: 711 1794 1223 2702
Fragments arranged by size:
2702 1794 1223 711
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3015 3126 3416 4237
Size: 53 83 186 936 1482 111 290 821
Cuts at: 4237 4619 5254 275
Size: 382 635 1451
Fragments arranged by size:
1482 1451 936 821 635 382 290 186
111 83 53
BsaWI w'CCGG_w
Cuts at: 4863 5261 5535 5682 6093 4863
Size: 398 274 147 411 5200
Fragments arranged by size:
5200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3146 3176 1178
Size: 30 165 30 1743 30 4432
Fragments arranged by size:
4432 1743 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3521 1186
Size: 2335 4095
BseYI C'CCAG_C
Cuts at: 2341 2691 2784 3018 3095 3243 3493 5633 6129
Size: 350 93 234 77 148 250 2140 496
Cuts at: 6129 2341
Size: 2642
Fragments arranged by size:
2642 2140 496 350 250 234 148 93
77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 3269 1163
Size: 573 471 1062 4324
Fragments arranged by size:
4324 1062 573 471
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2971 3880 4641 5669 1540
Size: 275 186 970 909 761 1028 2301
Fragments arranged by size:
2301 1028 970 909 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2507 3114 3192 4491 4576 5647 2507
Size: 607 78 1299 85 1071 3290
Fragments arranged by size:
3290 1299 1071 607 85 78
BsmI GAATG_Cn'
Cuts at: 2369 3941 4034 2369
Size: 1572 93 4765
Fragments arranged by size:
4765 1572 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2528 2818 3395 2528
Size: 290 577 5563
Fragments arranged by size:
5563 577 290
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2225 2242 2310 2507 2578 2585
Size: 399 277 158 17 68 197 71 7
Cuts at: 2585 3114 3122 3192 3251 3316 3749 4491 4576
Size: 529 8 70 59 65 433 742 85
Cuts at: 4576 5647 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 529 433 399 277 197
158 85 71 70 68 65 59 17
8 7
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 4319 6049 4319
Size: 1730 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 3288 3785 844
Size: 1502 942 497 3489
Fragments arranged by size:
3489 1502 942 497
BsrBI CCG'CTC
Cuts at: 1394 1823 4317 1394
Size: 429 2494 3507
Fragments arranged by size:
3507 2494 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2256 4925 5099 65
Size: 1183 1008 2669 174 1396
Fragments arranged by size:
2669 1396 1183 1008 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2950 3471 5074 5261 1700
Size: 110 1140 521 1603 187 2869
Fragments arranged by size:
2869 1603 1140 521 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 4490 5502 4490
Size: 1012 5418
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3522 794
Size: 2728 3702
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6430
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2680 2695 3038 3103 3361 2056
Size: 624 15 343 65 258 5125
Fragments arranged by size:
5125 624 343 258 65 15
BstYI r'GATC_y
Cuts at: 1972 3760 4127 4512 4529 5981 6067 6079 6425
Size: 1788 367 385 17 1452 86 12 346
Cuts at: 6425 1972
Size: 1977
Fragments arranged by size:
1977 1788 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2430 2430
Size: 6430
BtgI C'CryG_G
Cuts at: 513 1065 2004 2049 2269 2302 2751 2965 3535
Size: 552 939 45 220 33 449 214 570
Cuts at: 3535 513
Size: 3408
Fragments arranged by size:
3408 939 570 552 449 220 214 45
33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3010 3256 3529 487
Size: 683 56 1784 246 273 3388
Fragments arranged by size:
3388 1784 683 273 246 56
BtsI GCAGTG_nn'
Cuts at: 2353 3439 3946 4740 4760 2353
Size: 1086 507 794 20 4023
Fragments arranged by size:
4023 1086 794 507 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2810 5437 809
Size: 507 1494 2627 1802
Fragments arranged by size:
2627 1802 1494 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2326 2968 3040
Size: 54 1425 50 275 18 496 642 72
Cuts at: 3040 3571 3609 3877 4766 6395 8
Size: 531 38 268 889 1629 43
Fragments arranged by size:
1629 1425 889 642 531 496 275 268
72 54 50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 2968 3877 1537
Size: 275 1156 909 4090
Fragments arranged by size:
4090 1156 909 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2385 2553 4360 1353
Size: 1032 168 1807 3423
Fragments arranged by size:
3423 1807 1032 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5017 5391 5537 387
Size: 1064 138 3428 374 146 1280
Fragments arranged by size:
3428 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2505 2505
Size: 6430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2028 2159 2409 2552 2577 4493 5090
Size: 393 396 131 250 143 25 1916 597
Cuts at: 5090 5877 6145 1239
Size: 787 268 1524
Fragments arranged by size:
1916 1524 787 597 396 393 268 250
143 131 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2124 2124
Size: 6430
EcoO109I rG'GnC_Cy
Cuts at: 2039 2063 2092 2116 2132 2434 2475 2767 3148
Size: 24 29 24 16 302 41 292 381
Cuts at: 3148 3214 3293 3312 3313 2039
Size: 66 79 19 1 5156
Fragments arranged by size:
5156 381 302 292 79 66 41 29
24 24 19 16 1
EcoRI G'AATT_C
Cuts at: 3739 4117 3739
Size: 378 6052
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 3129 3307 5577 1678
Size: 1451 178 2270 2531
Fragments arranged by size:
2531 2270 1451 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2438 2470 2527 2559 3146
Size: 32 135 32 866 32 57 32 587
Cuts at: 3146 3178 3392 3424 5071 5103 5145 5177 1373
Size: 32 214 32 1647 32 42 32 2626
Fragments arranged by size:
2626 1647 866 587 214 135 57 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3774 4020 669
Size: 988 281 1836 246 3079
Fragments arranged by size:
3079 1836 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KasI G'GCGC_C
Cuts at: 3125 3125
Size: 6430
KpnI G_GTAC'C
Cuts at: 2680 3755 2680
Size: 1075 5355
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2129 2682 3189 5544 5728 1305
Size: 824 553 507 2355 184 2007
Fragments arranged by size:
2355 2007 824 553 507 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3042 3611 10
Size: 54 1425 343 1210 569 2829
Fragments arranged by size:
2829 1425 1210 569 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 2729 4388 4747 4906 518
Size: 648 912 651 1659 359 159 2042
Fragments arranged by size:
2042 1659 912 651 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2570 2695 2843 3392 3493 3683 3846 4523
Size: 964 125 148 549 101 190 163 677
Cuts at: 4523 5671 5916 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 964 677 549 245 190 163
148 125 101
NaeI GCC'GGC
Cuts at: 2952 2952
Size: 6430
NarI GG'CG_CC
Cuts at: 3126 3126
Size: 6430
NcoI C'CATG_G
Cuts at: 513 1065 2004 2049 2269 2751 513
Size: 552 939 45 220 482 4192
Fragments arranged by size:
4192 939 552 482 220 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NgoMIV G'CCGG_C
Cuts at: 2950 2950
Size: 6430
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 3788 5333 1132
Size: 97 2559 1545 2229
Fragments arranged by size:
2559 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2067 1348
Size: 719 5711
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2275 2570 1108
Size: 1167 295 4968
Fragments arranged by size:
4968 1167 295
PfoI T'CCnGG_A
Cuts at: 2649 2649
Size: 6430
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 6012 6044 4499
Size: 32 1481 32 4885
Fragments arranged by size:
4885 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2092 2116 2434 3148 2092
Size: 24 318 714 5374
Fragments arranged by size:
5374 714 318 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2063 2221 3312 2063
Size: 158 1091 5181
Fragments arranged by size:
5181 1091 158
PstI C_TGCA'G
Cuts at: 830 3527 3782 830
Size: 2697 255 3478
Fragments arranged by size:
3478 2697 255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2695 3493 3683 2695
Size: 798 190 5442
Fragments arranged by size:
5442 798 190
SacI G_AGCT'C
Cuts at: 2507 2507
Size: 6430
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SanDI GG'GwC_CC
Cuts at: 2116 2116
Size: 6430
SapI GCTCTTCn'nnn_
Cuts at: 2385 2553 2385
Size: 168 6262
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SexAI A'CCwGG_T
Cuts at: 3726 3726
Size: 6430
SfcI C'TryA_G
Cuts at: 826 1045 3061 3523 3778 4913 5594 5785 826
Size: 219 2016 462 255 1135 681 191 1471
Fragments arranged by size:
2016 1471 1135 681 462 255 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2747 2747
Size: 6430
SfoI GGC'GCC
Cuts at: 3127 3127
Size: 6430
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6430
SmaI CCC'GGG
Cuts at: 1872 2744 3757 1872
Size: 872 1013 4545
Fragments arranged by size:
4545 1013 872
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2506 3194 4534 5435 5697 820
Size: 197 926 563 688 1340 901 262 1553
Fragments arranged by size:
1553 1340 926 901 688 563 262 197
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3788 1132
Size: 2656 3774
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 3047 3650 1517
Size: 1530 603 4297
Fragments arranged by size:
4297 1530 603
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2269 2751 3869 513
Size: 552 939 45 220 482 1118 3074
Fragments arranged by size:
3074 1118 939 552 482 220 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2455 2705 4457 4474 4627 4812 5257
Size: 1255 875 250 1752 17 153 185 445
Cuts at: 5257 325
Size: 1498
Fragments arranged by size:
1752 1498 1255 875 445 250 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4676 6388 96
Size: 274 80 33 51 1443 2699 1712 138
Fragments arranged by size:
2699 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6390 6422 6390
Size: 32 6398
Tth111I GACn'n_nGTC
Cuts at: 1316 2657 2759 3406 1316
Size: 1341 102 647 4340
Fragments arranged by size:
4340 1341 647 102
XbaI T'CTAG_A
Cuts at: 2398 3766 2398
Size: 1368 5062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2621 2728 2797 3140 2621
Size: 107 69 343 5911
Fragments arranged by size:
5911 343 107 69
XhoI C'TCGA_G
Cuts at: 1943 3194 1943
Size: 1251 5179
XmaI C'CCGG_G
Cuts at: 1870 2742 3755 1870
Size: 872 1013 4545
Fragments arranged by size:
4545 1013 872
XmnI GAAnn'nnTTC
Cuts at: 4559 4559
Size: 6430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3417 4238 276
Size: 53 83 186 936 1883 821 2468
Fragments arranged by size:
2468 1883 936 821 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SrfI SwaI