Restriction map for
pFN21AE5683
(Circular) (Six-base) MAPSORT of: pFN21AE5683.seq Check: 606 from: 1 to: 6361
pFN21AE5683 6361 bp
With 182 enzymes: SgfI *
June 11, 2014 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4171 278
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3704 1937
Size: 1767 4594
Acc65I G'GTAC_C
Cuts at: 3682 3682
Size: 6361
AclI AA'CG_TT
Cuts at: 1563 3551 4488 4861 1563
Size: 1988 937 373 3063
Fragments arranged by size:
3063 1988 937 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2389 2581 4424 5808 6076 1632
Size: 757 192 1843 1384 268 1917
Fragments arranged by size:
1917 1843 1384 757 268 192
AflII C'TTAA_G
Cuts at: 820 1017 2299 820
Size: 197 1282 4882
Fragments arranged by size:
4882 1282 197
AflIII A'CryG_T
Cuts at: 1203 2512 3333 5260 6237 1203
Size: 1309 821 1927 977 1327
Fragments arranged by size:
1927 1327 1309 977 821
AgeI A'CCGG_T
Cuts at: 5192 5192
Size: 6361
AhdI GACnn_n'nnGTC
Cuts at: 5090 5090
Size: 6361
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6361
AlwNI CAG_nnn'CTG
Cuts at: 4104 5676 4104
Size: 1572 4789
ApaLI G'TGCA_C
Cuts at: 4418 5574 4418
Size: 1156 5205
ApoI r'AATT_y
Cuts at: 1493 2912 3210 3670 3896 4048 6157 1493
Size: 1419 298 460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1419 460 298 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4915 160
Size: 872 3883 1606
Fragments arranged by size:
3883 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
AvaI C'yCGr_G
Cuts at: 1870 1943 3686 1870
Size: 73 1743 4545
Fragments arranged by size:
4545 1743 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2527 2560 2527
Size: 33 6328
BamHI G'GATC_C
Cuts at: 3691 4058 3691
Size: 367 5994
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 3682 5137 618
Size: 325 217 627 366 1529 1455 1842
Fragments arranged by size:
1842 1529 1455 627 366 325 217
BanII G_rGCy'C
Cuts at: 1391 3680 1391
Size: 2289 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3298 928
Size: 971 1399 3991
Fragments arranged by size:
3991 1399 971
BbvCI CC'TCA_GC
Cuts at: 2768 2768
Size: 6361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2947 2981 2991 3025 4552
Size: 34 50 34 926 34 10 34 1527
Cuts at: 4552 4586 1903
Size: 34 3678
Fragments arranged by size:
3678 1527 926 50 34 34 34 34
34 10
BciVI GTATCCnnnnn_n'
Cuts at: 2039 4253 5463 2039
Size: 2214 1210 2937
Fragments arranged by size:
2937 2214 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6361
BfrBI ATG'CAT
Cuts at: 2008 3563 2008
Size: 1555 4806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2162 4972 136
Size: 107 122 71 631 605 490 2810 1525
Fragments arranged by size:
2810 1525 631 605 490 122 107 71
BglII A'GATC_T
Cuts at: 6356 6356
Size: 6361
BlpI GC'TnA_GC
Cuts at: 2969 3778 2969
Size: 809 5552
Bme1580I G_kGCm'C
Cuts at: 1790 3398 4422 5578 1790
Size: 1608 1024 1156 2573
Fragments arranged by size:
2573 1608 1156 1024
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6361
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2615 4092 5050
Size: 416 53 83 399 24 1198 1477 958
Cuts at: 5050 442
Size: 1753
Fragments arranged by size:
1753 1477 1198 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6361
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2084 2084
Size: 6361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5021 1239
Size: 3782 2579
Bpu10I CC'TnA_GC
Cuts at: 2768 2768
Size: 6361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4486 5351 5649 4486
Size: 865 298 5198
Fragments arranged by size:
5198 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3260 5024 882
Size: 617 1761 1764 2219
Fragments arranged by size:
2219 1764 1761 617
BsaAI yAC'GTr
Cuts at: 493 1204 2515 4152 493
Size: 711 1311 1637 2702
Fragments arranged by size:
2702 1637 1311 711
BsaBI GATnn'nnATC
Cuts at: 2399 6355 2399
Size: 3956 2405
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4168 4550 5185 275
Size: 53 83 186 936 2635 382 635 1451
Fragments arranged by size:
2635 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4794 5192 5466 5613 6024 4794
Size: 398 274 147 411 5131
Fragments arranged by size:
5131 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2855 2885 1178
Size: 30 165 30 1452 30 4654
Fragments arranged by size:
4654 1452 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 3215 1186
Size: 874 1155 4332
Fragments arranged by size:
4332 1155 874
BseYI C'CCAG_C
Cuts at: 2659 3386 5564 6060 2659
Size: 727 2178 496 2960
Fragments arranged by size:
2960 2178 727 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5788
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3811 4572 5600 1540
Size: 275 186 1810 761 1028 2301
Fragments arranged by size:
2301 1810 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 2605 4422 4507 5578 2208
Size: 397 1817 85 1071 2991
Fragments arranged by size:
2991 1817 1071 397 85
BsmI GAATG_Cn'
Cuts at: 3872 3965 3872
Size: 93 6268
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2605 3398 3680 4422 4507 5578
Size: 399 418 397 793 282 742 85 1071
Cuts at: 5578 1391
Size: 2174
Fragments arranged by size:
2174 1071 793 742 418 399 397 282
85
BspEI T'CCGG_A
Cuts at: 6024 6024
Size: 6361
BspHI T'CATG_A
Cuts at: 4250 5980 4250
Size: 1730 4631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3716 844
Size: 2872 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4248 1394
Size: 429 2425 3507
Fragments arranged by size:
3507 2425 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3108 3174 4856 5030 65
Size: 1183 1860 66 1682 174 1396
Fragments arranged by size:
1860 1682 1396 1183 174 66
BsrFI r'CCGG_y
Cuts at: 1700 1810 5005 5192 1700
Size: 110 3195 187 2869
Fragments arranged by size:
3195 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2586 2626 2862 3093 96
Size: 2490 40 236 231 3364
Fragments arranged by size:
3364 2490 236 231 40
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6361
BssSI C'ACGA_G
Cuts at: 4421 5433 4421
Size: 1012 5349
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6361
BstBI TT'CG_AA
Cuts at: 5165 5165
Size: 6361
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6361
BstYI r'GATC_y
Cuts at: 1972 2287 2698 2757 3691 4058 4443 4460 5912
Size: 315 411 59 934 367 385 17 1452
Cuts at: 5912 5998 6010 6356 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 934 411 385 367 346 315
86 59 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2703 513
Size: 552 1638 4171
Fragments arranged by size:
4171 1638 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2254 487
Size: 683 56 1028 4594
Fragments arranged by size:
4594 1028 683 56
BtsI GCAGTG_nn'
Cuts at: 2779 3877 4671 4691 2779
Size: 1098 794 20 4449
Fragments arranged by size:
4449 1098 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6361
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2038 2073 3595 3630 529
Size: 35 1474 35 1522 35 3260
Fragments arranged by size:
3260 1522 1474 35 35 35
DraI TTT'AAA
Cuts at: 2739 3665 2739
Size: 926 5435
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2488 5368 809
Size: 507 1172 2880 1802
Fragments arranged by size:
2880 1802 1172 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3808 4697 6326
Size: 54 1425 50 275 18 1978 889 1629
Cuts at: 6326 8
Size: 43
Fragments arranged by size:
1978 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3808 1537
Size: 275 1996 4090
Fragments arranged by size:
4090 1996 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3200 3233 4291 1353
Size: 1847 33 1058 3423
Fragments arranged by size:
3423 1847 1058 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3637 4948 5322 5468 387
Size: 1064 138 2048 1311 374 146 1280
Fragments arranged by size:
2048 1311 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2389 2581 4424 5021 5808 6076 1239
Size: 393 757 192 1843 597 787 268 1524
Fragments arranged by size:
1843 1524 787 757 597 393 268 192
EcoO109I rG'GnC_Cy
Cuts at: 2471 2808 2471
Size: 337 6024
EcoRI G'AATT_C
Cuts at: 3670 4048 3670
Size: 378 5983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2402 2473 3094 2402
Size: 71 621 5669
Fragments arranged by size:
5669 621 71
FspI TGC'GCA
Cuts at: 4867 4867
Size: 6361
HaeII r_GCGC'y
Cuts at: 1678 5508 1678
Size: 3830 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2357 2389 2648 2680 3279
Size: 32 135 32 785 32 259 32 599
Cuts at: 3279 3311 5002 5034 5076 5108 1373
Size: 32 1691 32 42 32 2626
Fragments arranged by size:
2626 1691 785 599 259 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3237 3705 3951 669
Size: 988 281 1299 468 246 3079
Fragments arranged by size:
3079 1299 988 468 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6361
HpaI GTT'AAC
Cuts at: 3237 3951 3237
Size: 714 5647
KpnI G_GTAC'C
Cuts at: 3686 3686
Size: 6361
MfeI C'AATT_G
Cuts at: 2522 3366 3960 2522
Size: 844 594 4923
Fragments arranged by size:
4923 844 594
MluI A'CGCG_T
Cuts at: 6237 6237
Size: 6361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2559 2689 3044 3526 5475 5659 1305
Size: 1254 130 355 482 1949 184 2007
Fragments arranged by size:
2007 1949 1254 482 355 184 130
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4539
Fragments arranged by size:
4539 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4319 4678 4837 518
Size: 648 3153 359 159 2042
Fragments arranged by size:
3153 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 3279 3777 4454 5602 5847 1606
Size: 481 1192 498 677 1148 245 2120
Fragments arranged by size:
2120 1192 1148 677 498 481 245
NcoI C'CATG_G
Cuts at: 513 1065 2703 513
Size: 552 1638 4171
Fragments arranged by size:
4171 1638 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6361
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6361
NotI GC'GGCC_GC
Cuts at: 3808 3808
Size: 6361
NsiI A_TGCA'T
Cuts at: 2010 3565 2010
Size: 1555 4806
NspI r_CATG'y
Cuts at: 1132 1229 3337 3567 3719 5264 1132
Size: 97 2108 230 152 1545 2229
Fragments arranged by size:
2229 2108 1545 230 152 97
PasI CC'CwG_GG
Cuts at: 1348 2339 1348
Size: 991 5370
PciI A'CATG_T
Cuts at: 3333 5260 3333
Size: 1927 4434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6361
PfoI T'CCnGG_A
Cuts at: 2753 2753
Size: 6361
PmeI GTTT'AAAC
Cuts at: 3665 3665
Size: 6361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4430 4462 5943 5975 4430
Size: 32 1481 32 4816
Fragments arranged by size:
4816 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2471 2471
Size: 6361
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6361
PsiI TTA'TAA
Cuts at: 3495 3931 3495
Size: 436 5925
PstI C_TGCA'G
Cuts at: 830 3713 830
Size: 2883 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6361
PvuII CAG'CTG
Cuts at: 2087 3279 2087
Size: 1192 5169
SalI G'TCGA_C
Cuts at: 1936 3703 1936
Size: 1767 4594
SanDI GG'GwC_CC
Cuts at: 2471 2471
Size: 6361
SapI GCTCTTCn'nnn_
Cuts at: 3200 3200
Size: 6361
SbfI CC_TGCA'GG
Cuts at: 3713 3713
Size: 6361
ScaI AGT'ACT
Cuts at: 4609 6321 4609
Size: 1712 4649
SfcI C'TryA_G
Cuts at: 826 1045 3027 3709 4844 5525 5716 826
Size: 219 1982 682 1135 681 191 1471
Fragments arranged by size:
1982 1471 1135 682 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6361
SmaI CCC'GGG
Cuts at: 1872 3688 1872
Size: 1816 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2299 4465 5366 5628 820
Size: 197 926 356 2166 901 262 1553
Fragments arranged by size:
2166 1553 926 901 356 262 197
SnaBI TAC'GTA
Cuts at: 493 4152 493
Size: 3659 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6361
SphI G_CATG'C
Cuts at: 1132 3567 3719 1132
Size: 2435 152 3774
Fragments arranged by size:
3774 2435 152
SspI AAT'ATT
Cuts at: 5 52 4285 5
Size: 47 4233 2081
Fragments arranged by size:
4233 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6361
StyI C'CwwG_G
Cuts at: 513 1065 2074 2703 3800 513
Size: 552 1009 629 1097 3074
Fragments arranged by size:
3074 1097 1009 629 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2817 3378 3461 4388 4405 4558 4743
Size: 1255 1237 561 83 927 17 153 185
Cuts at: 4743 5188 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1237 927 561 445 185 153
83 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2586 2626 2862
Size: 274 80 33 51 1443 609 40 236
Cuts at: 2862 3093 4607 6319 96
Size: 231 1514 1712 138
Fragments arranged by size:
1712 1514 1443 609 274 236 231 138
80 51 40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2601 2633 6321 6353 2601
Size: 32 3688 32 2609
Fragments arranged by size:
3688 2609 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6361
XbaI T'CTAG_A
Cuts at: 3697 3697
Size: 6361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2933 3450 2933
Size: 517 5844
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6361
XmaI C'CCGG_G
Cuts at: 1870 3686 1870
Size: 1816 4545
XmnI GAAnn'nnTTC
Cuts at: 2456 4490 2456
Size: 2034 4327
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4169 276
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI
SfiI SfoI SrfI SwaI