Restriction map for
pFN21AE5678
(Circular) (Six-base) MAPSORT of: pFN21AE5678.seq Check: 2235 from: 1 to: 6118
pFN21AE5678 6118 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3370 3370
Size: 6118
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3461 1937
Size: 1524 4594
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2879 3298 4181 5565 5833 1632
Size: 1247 419 883 1384 268 1917
Fragments arranged by size:
1917 1384 1247 883 419 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 5017 5994 1203
Size: 3814 977 1327
Fragments arranged by size:
3814 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 2677 4847 2677
Size: 2170 3948
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6118
AlwNI CAG_nnn'CTG
Cuts at: 2086 2587 3037 3861 5433 2086
Size: 501 450 824 1572 2771
Fragments arranged by size:
2771 1572 824 501 450
ApaI G_GGCC'C
Cuts at: 2293 2293
Size: 6118
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2157 3427 3653 3805 5914 1493
Size: 664 1270 226 152 2109 1697
Fragments arranged by size:
2109 1697 1270 664 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 3443 1870
Size: 73 1500 4545
Fragments arranged by size:
4545 1500 73
AvrII C'CTAG_G
Cuts at: 2285 2683 2285
Size: 398 5720
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3104 3439 4894 618
Size: 325 217 627 1317 335 1455 1842
Fragments arranged by size:
1842 1455 1317 627 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2293 3437 1391
Size: 902 1144 4072
Fragments arranged by size:
4072 1144 902
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2708 4010 5220 2708
Size: 1302 1210 3606
Fragments arranged by size:
3606 1302 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4729 136
Size: 107 122 71 631 605 3057 1525
Fragments arranged by size:
3057 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2820 6113 2820
Size: 3293 2825
BlpI GC'TnA_GC
Cuts at: 2955 3535 2955
Size: 580 5538
Bme1580I G_kGCm'C
Cuts at: 1790 2293 4179 5335 1790
Size: 503 1886 1156 2573
Fragments arranged by size:
2573 1886 1156 503
BmgBI CAC'GTC
Cuts at: 1318 3402 1318
Size: 2084 4034
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2489 2548 3849
Size: 416 53 83 399 24 1072 59 1301
Cuts at: 3849 4807 442
Size: 958 1753
Fragments arranged by size:
1753 1301 1072 958 416 399 83 59
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2609 3240 4778 1239
Size: 1370 631 1538 2579
Fragments arranged by size:
2579 1538 1370 631
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2121 2435 4243 5108 5406 2121
Size: 314 1808 865 298 2833
Fragments arranged by size:
2833 1808 865 314 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2439 4781 882
Size: 617 940 2342 2219
Fragments arranged by size:
2342 2219 940 617
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2645 3925 4307 4942
Size: 53 83 186 936 1112 1280 382 635
Cuts at: 4942 275
Size: 1451
Fragments arranged by size:
1451 1280 1112 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2869 4551 4949 5223 5370 5781 2869
Size: 1682 398 274 147 411 3206
Fragments arranged by size:
3206 1682 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5893
Fragments arranged by size:
5893 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2888 1186
Size: 841 861 4416
Fragments arranged by size:
4416 861 841
BseYI C'CCAG_C
Cuts at: 2086 5321 5817 2086
Size: 3235 496 2387
Fragments arranged by size:
3235 2387 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 3044 1163
Size: 573 447 861 4237
Fragments arranged by size:
4237 861 573 447
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4179 4264 5335 4179
Size: 85 1071 4962
Fragments arranged by size:
4962 1071 85
BsmI GAATG_Cn'
Cuts at: 3287 3629 3722 3287
Size: 342 93 5683
Fragments arranged by size:
5683 342 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2293 3437 4179 4264 5335 1391
Size: 399 503 1144 742 85 1071 2174
Fragments arranged by size:
2174 1144 1071 742 503 399 85
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 2778 4007 5737 2778
Size: 1229 1730 3159
Fragments arranged by size:
3159 1730 1229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3370 3473 844
Size: 2526 103 3489
Fragments arranged by size:
3489 2526 103
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2433 4613 4787 65
Size: 1183 1185 2180 174 1396
Fragments arranged by size:
2180 1396 1185 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2355 4762 4949 1700
Size: 110 545 2407 187 2869
Fragments arranged by size:
2869 2407 545 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2981 794
Size: 2187 3931
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstXI CCAn_nnnn'nTGG
Cuts at: 3146 3146
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 2820 3034 3448 3815 4200 4217 5669 5755
Size: 848 214 414 367 385 17 1452 86
Cuts at: 5755 5767 6113 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 848 414 385 367 346 214
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5566
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 2982 3634 4428 4448 2982
Size: 652 794 20 4652
Fragments arranged by size:
4652 794 652 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DraIII CAC_nnn'GTG
Cuts at: 3067 3067
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2832 3015 3565
Size: 54 1425 50 275 18 1002 183 550
Cuts at: 3565 4454 6083 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1002 889 550 275 183 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2802 3083 3384 4048 1353
Size: 1449 281 301 664 3423
Fragments arranged by size:
3423 1449 664 301 281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2609 2879 3240 3298 4181 4778 5565
Size: 393 977 270 361 58 883 597 787
Cuts at: 5565 5833 1239
Size: 268 1524
Fragments arranged by size:
1524 977 883 787 597 393 361 270
268 58
EcoO109I rG'GnC_Cy
Cuts at: 2289 2929 2289
Size: 640 5478
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
FspI TGC'GCA
Cuts at: 2439 4624 2439
Size: 2185 3933
HaeII r_GCGC'y
Cuts at: 1678 5265 1678
Size: 3587 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2663 2695 2871 2903 4759
Size: 32 135 32 1091 32 176 32 1856
Cuts at: 4759 4791 4833 4865 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1856 1091 176 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3708 669
Size: 988 281 1524 246 3079
Fragments arranged by size:
3079 1524 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6118
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 2071 3717 2071
Size: 1646 4472
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 5232 5416 1305
Size: 684 3243 184 2007
Fragments arranged by size:
3243 2007 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2834 3017 10
Size: 54 1425 343 1002 183 3111
Fragments arranged by size:
3111 1425 1002 343 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3138 3144 4076 4435 4594 518
Size: 648 1972 6 932 359 159 2042
Fragments arranged by size:
2042 1972 932 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2959 3318 3534 4211 5359 5604 1606
Size: 1353 359 216 677 1148 245 2120
Fragments arranged by size:
2120 1353 1148 677 359 245 216
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5566
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 3476 5021 1132
Size: 97 2247 1545 2229
Fragments arranged by size:
2247 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2587 1108
Size: 1479 4639
PfoI T'CCnGG_A
Cuts at: 3030 3030
Size: 6118
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PspOMI G'GGCC_C
Cuts at: 2289 2289
Size: 6118
PstI C_TGCA'G
Cuts at: 830 3309 3470 830
Size: 2479 161 3478
Fragments arranged by size:
3478 2479 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 2959 3318 2959
Size: 359 5759
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SapI GCTCTTCn'nnn_
Cuts at: 3384 3384
Size: 6118
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SfcI C'TryA_G
Cuts at: 826 1045 3305 3466 4601 5282 5473 826
Size: 219 2260 161 1135 681 191 1471
Fragments arranged by size:
2260 1471 1135 681 219 191 161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2136 2450 4222 5123 5385 820
Size: 197 926 193 314 1772 901 262 1553
Fragments arranged by size:
1772 1553 926 901 314 262 197 193
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6118
StyI C'CwwG_G
Cuts at: 513 1065 2285 2533 2683 3171 3557 513
Size: 552 1220 248 150 488 386 3074
Fragments arranged by size:
3074 1220 552 488 386 248 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2235 4145 4162 4315 4500 4945 325
Size: 1255 655 1910 17 153 185 445 1498
Fragments arranged by size:
1910 1498 1255 655 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4364 6076 96
Size: 274 80 33 51 1443 2387 1712 138
Fragments arranged by size:
2387 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3313 3345 6078 6110 3313
Size: 32 2733 32 3321
Fragments arranged by size:
3321 2733 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3122 1316
Size: 1806 4312
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2808 2929 3169 2808
Size: 121 240 5757
Fragments arranged by size:
5757 240 121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 4247 4247
Size: 6118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI