Restriction map for
pFN21AE5676
(Circular) (Six-base) MAPSORT of: pFN21AE5676.seq Check: 2984 from: 1 to: 6442
pFN21AE5676 6442 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4252 278
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3785 1937
Size: 1848 4594
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2385 2931 3135 4505 5889 6157 1632
Size: 753 546 204 1370 1384 268 1917
Fragments arranged by size:
1917 1384 1370 753 546 268 204
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6245
AflIII A'CryG_T
Cuts at: 1203 2333 2416 2879 5341 6318 1203
Size: 1130 83 463 2462 977 1327
Fragments arranged by size:
2462 1327 1130 977 463 83
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 5171 5171
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1166 3441 1166
Size: 2275 4167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6442
AlwNI CAG_nnn'CTG
Cuts at: 2627 4185 5757 2627
Size: 1558 1572 3312
Fragments arranged by size:
3312 1572 1558
ApaLI G'TGCA_C
Cuts at: 4499 5655 4499
Size: 1156 5286
ApoI r'AATT_y
Cuts at: 1493 2179 2272 3263 3751 3977 4129 6238 1493
Size: 686 93 991 488 226 152 2109 1697
Fragments arranged by size:
2109 1697 991 686 488 226 152 93
AseI AT'TA_AT
Cuts at: 160 1032 4996 160
Size: 872 3964 1606
Fragments arranged by size:
3964 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 3767 1870
Size: 73 1824 4545
Fragments arranged by size:
4545 1824 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3171 3204 3171
Size: 33 6409
BamHI G'GATC_C
Cuts at: 3772 4139 3772
Size: 367 6075
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3763 5218 618
Size: 325 217 627 1976 1455 1842
Fragments arranged by size:
1976 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 3761 1391
Size: 646 1724 4072
Fragments arranged by size:
4072 1724 646
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2391 2703 2729 928
Size: 971 492 312 26 4641
Fragments arranged by size:
4641 971 492 312 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2684 2718 4633 4667 1903
Size: 34 747 34 1915 34 3678
Fragments arranged by size:
3678 1915 747 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3212 4334 5544 3212
Size: 1122 1210 4110
Fragments arranged by size:
4110 1210 1122
BclI T'GATC_A
Cuts at: 1548 3274 1548
Size: 1726 4716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5053 136
Size: 107 122 71 631 605 3381 1525
Fragments arranged by size:
3381 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2488 2673 3481 6437 2488
Size: 185 808 2956 2493
Fragments arranged by size:
2956 2493 808 185
BlpI GC'TnA_GC
Cuts at: 2027 3859 2027
Size: 1832 4610
Bme1580I G_kGCm'C
Cuts at: 1790 2848 4503 5659 1790
Size: 1058 1655 1156 2573
Fragments arranged by size:
2573 1655 1156 1058
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6442
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2725 3054 4173
Size: 416 53 83 399 24 1308 329 1119
Cuts at: 4173 5131 442
Size: 958 1753
Fragments arranged by size:
1753 1308 1119 958 416 399 329 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2414 3444 5102 1239
Size: 1175 1030 1658 2579
Fragments arranged by size:
2579 1658 1175 1030
Bpu10I CC'TnA_GC
Cuts at: 2620 2620
Size: 6442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4567 5432 5730 4567
Size: 865 298 5279
Fragments arranged by size:
5279 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5105 882
Size: 617 3606 2219
Fragments arranged by size:
3606 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4233 493
Size: 711 3029 2702
Fragments arranged by size:
3029 2702 711
BsaBI GATnn'nnATC
Cuts at: 3243 6436 3243
Size: 3193 3249
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4249 4631 5266 275
Size: 53 83 186 936 2716 382 635 1451
Fragments arranged by size:
2716 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2092 4875 5273 5547 5694 6105 2092
Size: 2783 398 274 147 411 2429
Fragments arranged by size:
2783 2429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6217
Fragments arranged by size:
6217 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2828 1186
Size: 1642 4800
BseYI C'CCAG_C
Cuts at: 5645 6141 5645
Size: 496 5946
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5869
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3892 4653 5681 1540
Size: 275 186 1891 761 1028 2301
Fragments arranged by size:
2301 1891 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2520 2621 4503 4588 5659 2520
Size: 101 1882 85 1071 3303
Fragments arranged by size:
3303 1882 1071 101 85
BsmI GAATG_Cn'
Cuts at: 2984 3953 4046 2984
Size: 969 93 5380
Fragments arranged by size:
5380 969 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2520 2621 2848 3761 4503 4588
Size: 399 247 483 101 227 913 742 85
Cuts at: 4588 5659 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 913 742 483 399 247 227
101 85
BspEI T'CCGG_A
Cuts at: 2092 6105 2092
Size: 4013 2429
BspHI T'CATG_A
Cuts at: 4331 6061 4331
Size: 1730 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3797 844
Size: 2953 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4329 1394
Size: 429 2506 3507
Fragments arranged by size:
3507 2506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3007 3252 3406 4937 5111 65
Size: 1183 1759 245 154 1531 174 1396
Fragments arranged by size:
1759 1531 1396 1183 245 174 154
BsrFI r'CCGG_y
Cuts at: 1700 1810 2894 5086 5273 1700
Size: 110 1084 2192 187 2869
Fragments arranged by size:
2869 2192 1084 187 110
BsrGI T'GTAC_A
Cuts at: 96 3113 96
Size: 3017 3425
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 4502 5514 4502
Size: 1012 5430
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6442
BstBI TT'CG_AA
Cuts at: 2694 5246 2694
Size: 2552 3890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6442
BstXI CCAn_nnnn'nTGG
Cuts at: 2536 3594 2536
Size: 1058 5384
BstYI r'GATC_y
Cuts at: 1972 2488 2673 2758 3072 3481 3772 4139 4524
Size: 516 185 85 314 409 291 367 385
Cuts at: 4524 4541 5993 6079 6091 6437 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 516 409 385 367 346 314
291 185 86 85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5890
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3720 487
Size: 683 56 2494 3209
Fragments arranged by size:
3209 2494 683 56
BtsI GCAGTG_nn'
Cuts at: 2787 3958 4752 4772 2787
Size: 1171 794 20 4457
Fragments arranged by size:
4457 1171 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2298 2333 3278 3313 529
Size: 35 1734 35 945 35 3658
Fragments arranged by size:
3658 1734 945 35 35 35
DraI TTT'AAA
Cuts at: 2500 2890 3746 2500
Size: 390 856 5196
Fragments arranged by size:
5196 856 390
DraIII CAC_nnn'GTG
Cuts at: 3228 3230 3228
Size: 2 6440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5449 809
Size: 507 4133 1802
Fragments arranged by size:
4133 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3596 3889 4778
Size: 54 1425 50 275 18 1766 293 889
Cuts at: 4778 6407 8
Size: 1629 43
Fragments arranged by size:
1766 1629 1425 889 293 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3889 1537
Size: 275 2077 4090
Fragments arranged by size:
4090 2077 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4372 1353
Size: 3019 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5029 5403 5549 387
Size: 1064 138 3440 374 146 1280
Fragments arranged by size:
3440 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2385 2414 2931 3135 3444 4505 5102
Size: 393 753 29 517 204 309 1061 597
Cuts at: 5102 5889 6157 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1061 787 753 597 517 393 309
268 204 29
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 3559 5589 1678
Size: 1881 2030 2531
Fragments arranged by size:
2531 2030 1881
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2465 2497 3034 3066 5083
Size: 32 135 32 893 32 537 32 2017
Cuts at: 5083 5115 5157 5189 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2017 893 537 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3786 4032 669
Size: 988 281 1848 246 3079
Fragments arranged by size:
3079 1848 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6442
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5556 5740 1305
Size: 4251 184 2007
Fragments arranged by size:
4251 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3598 10
Size: 54 1425 343 1766 2854
Fragments arranged by size:
2854 1766 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2661 3054 3257 3441 4400 4759 4918
Size: 648 1495 393 203 184 959 359 159
Cuts at: 4918 518
Size: 2042
Fragments arranged by size:
2042 1495 959 648 393 359 203 184
159
MspA1I CmG'CkG
Cuts at: 1606 2031 3858 4535 5683 5928 1606
Size: 425 1827 677 1148 245 2120
Fragments arranged by size:
2120 1827 1148 677 425 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5890
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6442
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NspI r_CATG'y
Cuts at: 1132 1229 2337 2420 2865 2883 3800 5345 1132
Size: 97 1108 83 445 18 917 1545 2229
Fragments arranged by size:
2229 1545 1108 917 445 97 83 18
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6442
PciI A'CATG_T
Cuts at: 2333 2416 2879 5341 2333
Size: 83 463 2462 3434
Fragments arranged by size:
3434 2462 463 83
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6442
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 6024 6056 4511
Size: 32 1481 32 4897
Fragments arranged by size:
4897 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6442
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PstI C_TGCA'G
Cuts at: 830 2690 3794 830
Size: 1860 1104 3478
Fragments arranged by size:
3478 1860 1104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SbfI CC_TGCA'GG
Cuts at: 3794 3794
Size: 6442
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SfcI C'TryA_G
Cuts at: 826 1045 2357 2686 3439 3525 3790 4925 5606
Size: 219 1312 329 753 86 265 1135 681
Cuts at: 5606 5797 826
Size: 191 1471
Fragments arranged by size:
1471 1312 1135 753 681 329 265 219
191 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 3769 1872
Size: 1897 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4546 5447 5709 820
Size: 197 926 2603 901 262 1553
Fragments arranged by size:
2603 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6442
SphI G_CATG'C
Cuts at: 1132 3800 1132
Size: 2668 3774
SspI AAT'ATT
Cuts at: 5 52 2996 4366 5
Size: 47 2944 1370 2081
Fragments arranged by size:
2944 2081 1370 47
StuI AGG'CCT
Cuts at: 1517 2965 1517
Size: 1448 4994
StyI C'CwwG_G
Cuts at: 513 1065 2287 3881 513
Size: 552 1222 1594 3074
Fragments arranged by size:
3074 1594 1222 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4469 4486 4639 4824 5269 325
Size: 1255 2889 17 153 185 445 1498
Fragments arranged by size:
2889 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2336 3113 3383
Size: 274 80 33 51 1443 359 777 270
Cuts at: 3383 3637 4688 6400 96
Size: 254 1051 1712 138
Fragments arranged by size:
1712 1443 1051 777 359 274 270 254
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6402 6434 6402
Size: 32 6410
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6442
XbaI T'CTAG_A
Cuts at: 3778 3778
Size: 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 3767 1870
Size: 1897 4545
XmnI GAAnn'nnTTC
Cuts at: 2652 4571 2652
Size: 1919 4523
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4250 276
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI