Restriction map for
pFN21AE5674
(Circular) (Six-base) MAPSORT of: pFN21AE5674.seq Check: 2933 from: 1 to: 6373
pFN21AE5674 6373 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4183 278
Size: 53 83 186 936 2647 2468
Fragments arranged by size:
2647 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3166 3716 1937
Size: 1229 550 4594
Fragments arranged by size:
4594 1229 550
Acc65I G'GTAC_C
Cuts at: 3694 3694
Size: 6373
AclI AA'CG_TT
Cuts at: 1563 4500 4873 1563
Size: 2937 373 3063
Fragments arranged by size:
3063 2937 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2531 2690 4436 5820 6088 1632
Size: 899 159 1746 1384 268 1917
Fragments arranged by size:
1917 1746 1384 899 268 159
AflII C'TTAA_G
Cuts at: 820 1017 2465 820
Size: 197 1448 4728
Fragments arranged by size:
4728 1448 197
AflIII A'CryG_T
Cuts at: 1203 3424 5272 6249 1203
Size: 2221 1848 977 1327
Fragments arranged by size:
2221 1848 1327 977
AgeI A'CCGG_T
Cuts at: 5204 5204
Size: 6373
AhdI GACnn_n'nnGTC
Cuts at: 5102 5102
Size: 6373
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3484 1184
Size: 2300 4073
AlwNI CAG_nnn'CTG
Cuts at: 2965 4116 5688 2965
Size: 1151 1572 3650
Fragments arranged by size:
3650 1572 1151
ApaLI G'TGCA_C
Cuts at: 4430 5586 4430
Size: 1156 5217
ApoI r'AATT_y
Cuts at: 1493 2081 2440 2450 2921 2982 3682 3908 4060
Size: 588 359 10 471 61 700 226 152
Cuts at: 4060 6169 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 700 588 471 359 226 152
61 10
AseI AT'TA_AT
Cuts at: 160 1032 2822 4927 160
Size: 872 1790 2105 1606
Fragments arranged by size:
2105 1790 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
AvaI C'yCGr_G
Cuts at: 1870 1943 3656 3698 1870
Size: 73 1713 42 4545
Fragments arranged by size:
4545 1713 73 42
BamHI G'GATC_C
Cuts at: 3703 4070 3703
Size: 367 6006
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3694 5149 618
Size: 325 217 627 1907 1455 1842
Fragments arranged by size:
1907 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2279 3692 1391
Size: 888 1413 4072
Fragments arranged by size:
4072 1413 888
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2519 3609 928
Size: 971 620 1090 3692
Fragments arranged by size:
3692 1090 971 620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4564 4598 1903
Size: 34 2627 34 3678
Fragments arranged by size:
3678 2627 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4265 5475 4265
Size: 1210 5163
BclI T'GATC_A
Cuts at: 1548 3030 3234 1548
Size: 1482 204 4687
Fragments arranged by size:
4687 1482 204
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4984 136
Size: 107 122 71 631 605 3312 1525
Fragments arranged by size:
3312 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2551 6368 2551
Size: 3817 2556
BlpI GC'TnA_GC
Cuts at: 3790 3790
Size: 6373
Bme1580I G_kGCm'C
Cuts at: 1790 4434 5590 1790
Size: 2644 1156 2573
Fragments arranged by size:
2644 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2316 1318
Size: 998 5375
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2282 2933 4104
Size: 416 53 83 399 24 865 651 1171
Cuts at: 4104 5062 442
Size: 958 1753
Fragments arranged by size:
1753 1171 958 865 651 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3636 1056
Size: 2580 3793
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2122 2181 3690 5033 1239
Size: 883 59 1509 1343 2579
Fragments arranged by size:
2579 1509 1343 883 59
Bpu10I CC'TnA_GC
Cuts at: 2013 2429 2013
Size: 416 5957
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4498 5363 5661 4498
Size: 865 298 5210
Fragments arranged by size:
5210 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5036 882
Size: 617 3537 2219
Fragments arranged by size:
3537 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4164 493
Size: 711 2960 2702
Fragments arranged by size:
2960 2702 711
BsaBI GATnn'nnATC
Cuts at: 6367 6367
Size: 6373
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4180 4562 5197 275
Size: 53 83 186 936 2647 382 635 1451
Fragments arranged by size:
2647 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2410 4806 5204 5478 5625 6036 2410
Size: 2396 398 274 147 411 2747
Fragments arranged by size:
2747 2396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2638 2668 2701 2731 1178
Size: 30 165 30 1235 30 33 30 4820
Fragments arranged by size:
4820 1235 165 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2860 3135 1186
Size: 856 818 275 4424
Fragments arranged by size:
4424 856 818 275
BseYI C'CCAG_C
Cuts at: 2928 3571 5576 6072 2928
Size: 643 2005 496 3229
Fragments arranged by size:
3229 2005 643 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 2959 3431 3454 1163
Size: 573 624 599 472 23 4082
Fragments arranged by size:
4082 624 599 573 472 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3823 4584 5612 1540
Size: 275 186 1822 761 1028 2301
Fragments arranged by size:
2301 1822 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4434 4519 5590 4434
Size: 85 1071 5217
Fragments arranged by size:
5217 1071 85
BsmI GAATG_Cn'
Cuts at: 3884 3977 3884
Size: 93 6280
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2279 3692 4434 4519 5590 1391
Size: 399 489 1413 742 85 1071 2174
Fragments arranged by size:
2174 1413 1071 742 489 399 85
BspEI T'CCGG_A
Cuts at: 2410 6036 2410
Size: 3626 2747
BspHI T'CATG_A
Cuts at: 4262 5992 4262
Size: 1730 4643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3630 3728 844
Size: 2786 98 3489
Fragments arranged by size:
3489 2786 98
BsrBI CCG'CTC
Cuts at: 1394 1823 4260 1394
Size: 429 2437 3507
Fragments arranged by size:
3507 2437 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2195 2238 3483 3585 4868 5042 65
Size: 1183 947 43 1245 102 1283 174 1396
Fragments arranged by size:
1396 1283 1245 1183 947 174 102 43
BsrFI r'CCGG_y
Cuts at: 1700 1810 5017 5204 1700
Size: 110 3207 187 2869
Fragments arranged by size:
3207 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2419 2799 96
Size: 2323 380 3670
Fragments arranged by size:
3670 2323 380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6373
BssSI C'ACGA_G
Cuts at: 4433 5445 4433
Size: 1012 5361
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2591 794
Size: 1797 4576
BstBI TT'CG_AA
Cuts at: 5177 5177
Size: 6373
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6373
BstYI r'GATC_y
Cuts at: 1972 2347 2551 3665 3703 4070 4455 4472 5924
Size: 375 204 1114 38 367 385 17 1452
Cuts at: 5924 6010 6022 6368 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1114 385 375 367 346 204
86 38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2386 513
Size: 552 1321 4500
Fragments arranged by size:
4500 1321 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5634
Fragments arranged by size:
5634 683 56
BtsI GCAGTG_nn'
Cuts at: 3118 3889 4683 4703 3118
Size: 771 794 20 4788
Fragments arranged by size:
4788 794 771 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6373
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6338
DraI TTT'AAA
Cuts at: 3677 3677
Size: 6373
DraIII CAC_nnn'GTG
Cuts at: 3247 3376 3247
Size: 129 6244
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5380 809
Size: 507 4064 1802
Fragments arranged by size:
4064 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3157 3208 3820
Size: 54 1425 50 275 18 1327 51 612
Cuts at: 3820 4709 6338 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1327 889 612 275 54 51
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3820 1537
Size: 275 2008 4090
Fragments arranged by size:
4090 2008 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 3108 3153 4303 1353
Size: 782 973 45 1150 3423
Fragments arranged by size:
3423 1150 973 782 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4960 5334 5480 387
Size: 1064 138 3371 374 146 1280
Fragments arranged by size:
3371 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2181 2531 2690 3690 4436 5033
Size: 393 490 59 350 159 1000 746 597
Cuts at: 5033 5820 6088 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1000 787 746 597 490 393 350
268 159 59
EcoNI CCTnn'n_nnAGG
Cuts at: 3134 3134
Size: 6373
EcoO109I rG'GnC_Cy
Cuts at: 2470 3055 2470
Size: 585 5788
EcoRI G'AATT_C
Cuts at: 2982 3682 4060 2982
Size: 700 378 5295
Fragments arranged by size:
5295 700 378
FspI TGC'GCA
Cuts at: 4879 4879
Size: 6373
HaeII r_GCGC'y
Cuts at: 1678 5520 1678
Size: 3842 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2041 2073 2638 2670 2701
Size: 32 135 32 469 32 565 32 31
Cuts at: 2701 2733 3650 3682 5014 5046 5088 5120 1373
Size: 32 917 32 1332 32 42 32 2626
Fragments arranged by size:
2626 1332 917 565 469 135 42 32
32 32 32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2845 3717 3963 669
Size: 988 281 907 872 246 3079
Fragments arranged by size:
3079 988 907 872 281 246
HindIII A'AGCT_T
Cuts at: 748 2117 748
Size: 1369 5004
HpaI GTT'AAC
Cuts at: 3963 3963
Size: 6373
KpnI G_GTAC'C
Cuts at: 3698 3698
Size: 6373
MfeI C'AATT_G
Cuts at: 2778 2811 3972 2778
Size: 33 1161 5179
Fragments arranged by size:
5179 1161 33
MluI A'CGCG_T
Cuts at: 6249 6249
Size: 6373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3435 5487 5671 1305
Size: 2130 2052 184 2007
Fragments arranged by size:
2130 2052 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4551
Fragments arranged by size:
4551 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2286 2622 3518 4331 4690 4849 518
Size: 648 1120 336 896 813 359 159 2042
Fragments arranged by size:
2042 1120 896 813 648 359 336 159
MspA1I CmG'CkG
Cuts at: 1606 2184 3789 4466 5614 5859 1606
Size: 578 1605 677 1148 245 2120
Fragments arranged by size:
2120 1605 1148 677 578 245
NcoI C'CATG_G
Cuts at: 513 1065 2386 513
Size: 552 1321 4500
Fragments arranged by size:
4500 1321 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6373
NheI G'CTAG_C
Cuts at: 1052 3632 1052
Size: 2580 3793
NotI GC'GGCC_GC
Cuts at: 3820 3820
Size: 6373
NspI r_CATG'y
Cuts at: 1132 1229 3428 3731 5276 1132
Size: 97 2199 303 1545 2229
Fragments arranged by size:
2229 2199 1545 303 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6373
PciI A'CATG_T
Cuts at: 3424 5272 3424
Size: 1848 4525
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2648 1108
Size: 1540 4833
PfoI T'CCnGG_A
Cuts at: 2061 2061
Size: 6373
PmeI GTTT'AAAC
Cuts at: 3677 3677
Size: 6373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2940 2972 4442 4474 5955 5987 2940
Size: 32 1470 32 1481 32 3326
Fragments arranged by size:
3326 1481 1470 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3055 3055
Size: 6373
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6373
PsiI TTA'TAA
Cuts at: 3943 3943
Size: 6373
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2038 2070 2657 2689 2038
Size: 32 587 32 5722
Fragments arranged by size:
5722 587 32 32
PstI C_TGCA'G
Cuts at: 830 2356 2970 3725 830
Size: 1526 614 755 3478
Fragments arranged by size:
3478 1526 755 614
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6373
PvuII CAG'CTG
Cuts at: 2184 2184
Size: 6373
SalI G'TCGA_C
Cuts at: 1936 3715 1936
Size: 1779 4594
SapI GCTCTTCn'nnn_
Cuts at: 3108 3108
Size: 6373
SbfI CC_TGCA'GG
Cuts at: 2356 3725 2356
Size: 1369 5004
ScaI AGT'ACT
Cuts at: 2675 4621 6333 2675
Size: 1946 1712 2715
Fragments arranged by size:
2715 1946 1712
SfcI C'TryA_G
Cuts at: 826 1045 2352 2966 3167 3721 4856 5537 5728
Size: 219 1307 614 201 554 1135 681 191
Cuts at: 5728 826
Size: 1471
Fragments arranged by size:
1471 1307 1135 681 614 554 219 201
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6373
SmaI CCC'GGG
Cuts at: 1872 3700 1872
Size: 1828 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2465 4477 5378 5640 820
Size: 197 926 522 2012 901 262 1553
Fragments arranged by size:
2012 1553 926 901 522 262 197
SnaBI TAC'GTA
Cuts at: 493 4164 493
Size: 3671 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6373
SphI G_CATG'C
Cuts at: 1132 3731 1132
Size: 2599 3774
SspI AAT'ATT
Cuts at: 5 52 4297 5
Size: 47 4245 2081
Fragments arranged by size:
4245 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6373
StyI C'CwwG_G
Cuts at: 513 1065 2386 2601 2642 3812 513
Size: 552 1321 215 41 1170 3074
Fragments arranged by size:
3074 1321 1170 552 215 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4400 4417 4570 4755 5200 325
Size: 1255 2820 17 153 185 445 1498
Fragments arranged by size:
2820 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2419 2523 2574
Size: 274 80 33 51 1443 442 104 51
Cuts at: 2574 2673 2799 4619 6331 96
Size: 99 126 1820 1712 138
Fragments arranged by size:
1820 1712 1443 442 274 138 126 104
99 80 51 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2968 3000 6333 6365 2968
Size: 32 3333 32 2976
Fragments arranged by size:
3333 2976 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6373
XbaI T'CTAG_A
Cuts at: 2945 3709 2945
Size: 764 5609
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6373
XmaI C'CCGG_G
Cuts at: 1870 3698 1870
Size: 1828 4545
XmnI GAAnn'nnTTC
Cuts at: 3555 4502 3555
Size: 947 5426
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4181 276
Size: 53 83 186 936 2647 2468
Fragments arranged by size:
2647 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI