Restriction map for
pFN21AE5673
(Circular) (Six-base) MAPSORT of: pFN21AE5673.seq Check: 6320 from: 1 to: 6334
pFN21AE5673 6334 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3677 1937
Size: 1740 4594
Acc65I G'GTAC_C
Cuts at: 2967 3655 2967
Size: 688 5646
AclI AA'CG_TT
Cuts at: 1563 2889 4461 4834 1563
Size: 1326 1572 373 3063
Fragments arranged by size:
3063 1572 1326 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3457 4397 5781 6049 1632
Size: 1825 940 1384 268 1917
Fragments arranged by size:
1917 1825 1384 940 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 5233 6210 1203
Size: 4030 977 1327
Fragments arranged by size:
4030 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 5063 5063
Size: 6334
AleI CACnn'nnGTG
Cuts at: 1166 2462 1166
Size: 1296 5038
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6334
AlwNI CAG_nnn'CTG
Cuts at: 2476 4077 5649 2476
Size: 1601 1572 3161
Fragments arranged by size:
3161 1601 1572
ApaLI G'TGCA_C
Cuts at: 4391 5547 4391
Size: 1156 5178
ApoI r'AATT_y
Cuts at: 1493 3093 3643 3869 4021 6130 1493
Size: 1600 550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1600 550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 3659 1870
Size: 73 1716 4545
Fragments arranged by size:
4545 1716 73
BamHI G'GATC_C
Cuts at: 3664 4031 3664
Size: 367 5967
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2559 2967 3655 5110 618
Size: 325 217 627 772 408 688 1455 1842
Fragments arranged by size:
1842 1455 772 688 627 408 325 217
BanII G_rGCy'C
Cuts at: 1391 2131 2259 3653 1391
Size: 740 128 1394 4072
Fragments arranged by size:
4072 1394 740 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5363
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4525 4559 1903
Size: 34 2588 34 3678
Fragments arranged by size:
3678 2588 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4226 5436 4226
Size: 1210 5124
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3014 4945 136
Size: 107 122 71 631 605 1342 1931 1525
Fragments arranged by size:
1931 1525 1342 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6329 6329
Size: 6334
BlpI GC'TnA_GC
Cuts at: 3751 3751
Size: 6334
Bme1580I G_kGCm'C
Cuts at: 1790 2564 3382 4395 5551 1790
Size: 774 818 1013 1156 2573
Fragments arranged by size:
2573 1156 1013 818 774
BmgBI CAC'GTC
Cuts at: 1318 3253 1318
Size: 1935 4399
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2909 4065 5023
Size: 416 53 83 399 24 1492 1156 958
Cuts at: 5023 442
Size: 1753
Fragments arranged by size:
1753 1492 1156 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2091 2453 2456 2975 4994 1239
Size: 852 362 3 519 2019 2579
Fragments arranged by size:
2579 2019 852 519 362 3
Bpu10I CC'TnA_GC
Cuts at: 3129 3129
Size: 6334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4459 5324 5622 4459
Size: 865 298 5171
Fragments arranged by size:
5171 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3038 4997 882
Size: 617 1539 1959 2219
Fragments arranged by size:
2219 1959 1539 617
BsaAI yAC'GTr
Cuts at: 493 1204 2113 4125 493
Size: 711 909 2012 2702
Fragments arranged by size:
2702 2012 909 711
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4141 4523 5158 275
Size: 53 83 186 936 2608 382 635 1451
Fragments arranged by size:
2608 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4767 5165 5439 5586 5997 4767
Size: 398 274 147 411 5104
Fragments arranged by size:
5104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6109
Fragments arranged by size:
6109 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 3165 1186
Size: 970 1009 4355
Fragments arranged by size:
4355 1009 970
BseYI C'CCAG_C
Cuts at: 3543 5537 6033 3543
Size: 1994 496 3844
Fragments arranged by size:
3844 1994 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3063 1163
Size: 573 1327 4434
Fragments arranged by size:
4434 1327 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3784 4545 5573 1540
Size: 275 186 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 2469 4395 4480 5551 2259
Size: 210 1926 85 1071 3042
Fragments arranged by size:
3042 1926 1071 210 85
BsmI GAATG_Cn'
Cuts at: 2346 2663 3055 3175 3845 3938 2346
Size: 317 392 120 670 93 4742
Fragments arranged by size:
4742 670 392 317 120 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2409 2409
Size: 6334
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2259 2469 2564 3382 3653 4395
Size: 399 341 128 210 95 818 271 742
Cuts at: 4395 4480 5551 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 818 742 399 341 271 210
128 95 85
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 2259 4223 5953 2259
Size: 1964 1730 2640
Fragments arranged by size:
2640 1964 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2709 3689 844
Size: 1865 980 3489
Fragments arranged by size:
3489 1865 980
BsrBI CCG'CTC
Cuts at: 1394 1823 4221 1394
Size: 429 2398 3507
Fragments arranged by size:
3507 2398 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4829 5003 65
Size: 1183 3581 174 1396
Fragments arranged by size:
3581 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4978 5165 1700
Size: 110 3168 187 2869
Fragments arranged by size:
3168 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2317 96
Size: 2221 4113
BssHII G'CGCG_C
Cuts at: 1925 2035 1925
Size: 110 6224
BssSI C'ACGA_G
Cuts at: 2162 4394 5406 2162
Size: 2232 1012 3090
Fragments arranged by size:
3090 2232 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3050 794
Size: 2256 4078
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstXI CCAn_nnnn'nTGG
Cuts at: 2817 2817
Size: 6334
BstYI r'GATC_y
Cuts at: 1972 2686 3664 4031 4416 4433 5885 5971 5983
Size: 714 978 367 385 17 1452 86 12
Cuts at: 5983 6329 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 978 714 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3508 3566 3508
Size: 58 6276
BtgI C'CryG_G
Cuts at: 513 1065 2004 3438 513
Size: 552 939 1434 3409
Fragments arranged by size:
3409 1434 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5595
Fragments arranged by size:
5595 683 56
BtsI GCAGTG_nn'
Cuts at: 2554 3850 4644 4664 2554
Size: 1296 794 20 4224
Fragments arranged by size:
4224 1296 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3090 3638 3090
Size: 548 5786
DraIII CAC_nnn'GTG
Cuts at: 3159 3159
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 809
Size: 507 4025 1802
Fragments arranged by size:
4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2487 2514 3015
Size: 54 1425 50 275 18 657 27 501
Cuts at: 3015 3781 4670 6299 8
Size: 766 889 1629 43
Fragments arranged by size:
1629 1425 889 766 657 501 275 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4264 1353
Size: 2911 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2043 4921 5295 5441 387
Size: 1064 138 454 2878 374 146 1280
Fragments arranged by size:
2878 1280 1064 454 374 146 138
EcoICRI GAG'CTC
Cuts at: 2257 2257
Size: 6334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2453 2456 2975 3457 4397 4994
Size: 393 459 362 3 519 482 940 597
Cuts at: 4994 5781 6049 1239
Size: 787 268 1524
Fragments arranged by size:
1524 940 787 597 519 482 459 393
362 268 3
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FspI TGC'GCA
Cuts at: 2016 4840 2016
Size: 2824 3510
FspAI rTGC'GCAy
Cuts at: 2016 2016
Size: 6334
HaeII r_GCGC'y
Cuts at: 1678 2476 3619 5481 1678
Size: 798 1143 1862 2531
Fragments arranged by size:
2531 1862 1143 798
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2040 2072 2186 2218 3411
Size: 32 135 32 468 32 114 32 1193
Cuts at: 3411 3443 3589 3621 4975 5007 5049 5081 1373
Size: 32 146 32 1354 32 42 32 2626
Fragments arranged by size:
2626 1354 1193 468 146 135 114 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2626 3678 3924 669
Size: 988 281 688 1052 246 3079
Fragments arranged by size:
3079 1052 988 688 281 246
HindIII A'AGCT_T
Cuts at: 748 2084 2862 748
Size: 1336 778 4220
Fragments arranged by size:
4220 1336 778
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KpnI G_GTAC'C
Cuts at: 2971 3659 2971
Size: 688 5646
MfeI C'AATT_G
Cuts at: 2847 3933 2847
Size: 1086 5248
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5448 5632 1305
Size: 4143 184 2007
Fragments arranged by size:
4143 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2489 2516 3017 10
Size: 54 1425 343 657 27 501 3327
Fragments arranged by size:
3327 1425 657 501 343 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 2547 3144 3371 4292 4651 4810
Size: 648 1296 85 597 227 921 359 159
Cuts at: 4810 518
Size: 2042
Fragments arranged by size:
2042 1296 921 648 597 359 227 159
85
MspA1I CmG'CkG
Cuts at: 1606 3750 4427 5575 5820 1606
Size: 2144 677 1148 245 2120
Fragments arranged by size:
2144 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4843
Fragments arranged by size:
4843 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 2015 2739 2801 3692 5237 1132
Size: 97 786 724 62 891 1545 2229
Fragments arranged by size:
2229 1545 891 786 724 97 62
PasI CC'CwG_GG
Cuts at: 1348 2122 3322 1348
Size: 774 1200 4360
Fragments arranged by size:
4360 1200 774
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2342 2570 2977 3159 1108
Size: 1234 228 407 182 4283
Fragments arranged by size:
4283 1234 407 228 182
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5916 5948 4403
Size: 32 1481 32 4789
Fragments arranged by size:
4789 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3002 3904 3002
Size: 902 5432
PstI C_TGCA'G
Cuts at: 830 2305 3686 830
Size: 1475 1381 3478
Fragments arranged by size:
3478 1475 1381
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
SacI G_AGCT'C
Cuts at: 2259 2259
Size: 6334
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 3478 4582 6294 3478
Size: 1104 1712 3518
Fragments arranged by size:
3518 1712 1104
SfcI C'TryA_G
Cuts at: 826 1045 2301 3682 4817 5498 5689 826
Size: 219 1256 1381 1135 681 191 1471
Fragments arranged by size:
1471 1381 1256 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 3661 1872
Size: 1789 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4438 5339 5601 820
Size: 197 926 2495 901 262 1553
Fragments arranged by size:
2495 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 2015 3692 1132
Size: 883 1677 3774
Fragments arranged by size:
3774 1677 883
SspI AAT'ATT
Cuts at: 5 52 2174 4258 5
Size: 47 2122 2084 2081
Fragments arranged by size:
2122 2084 2081 47
StuI AGG'CCT
Cuts at: 1517 2249 1517
Size: 732 5602
StyI C'CwwG_G
Cuts at: 513 1065 2004 2250 2555 3773 513
Size: 552 939 246 305 1218 3074
Fragments arranged by size:
3074 1218 939 552 305 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2928 4361 4378 4531 4716 5161 325
Size: 1255 1348 1433 17 153 185 445 1498
Fragments arranged by size:
1498 1433 1348 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2317 3349 3476
Size: 274 80 33 51 1443 340 1032 127
Cuts at: 3476 4580 6292 96
Size: 1104 1712 138
Fragments arranged by size:
1712 1443 1104 1032 340 274 138 127
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 6294 6326 3242
Size: 32 3020 32 3250
Fragments arranged by size:
3250 3020 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3025 3025
Size: 6334
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6334
XmaI C'CCGG_G
Cuts at: 1870 3659 1870
Size: 1789 4545
XmnI GAAnn'nnTTC
Cuts at: 4463 4463
Size: 6334
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I EcoNI EcoO109I EcoRV
FalI FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI